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Kırşehir İlinden Toplanan Morchella esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu ve Antimikrobiyal Aktivitelerinin Belirlenmesi

Year 2021, Volume: 7 Issue: 1, 48 - 59, 20.03.2021

Elif Sevim , Ali Sevim

https://doi.org/10.28979/jarnas.890330
Cited By: 1

Abstract

Doğal yenilebilir mantarların Dünya’nın birçok ülkesinde geleneksel anlamda besin ve ilaç olarak kullanılıldığı uzun zamandan beri bilinen bir konudur. Morchella esculenta (L.) Pers (kuzu göbeği) tıbbi kullanım ve besin değeri açısından son derece değerli bulunmaktadır. Günümüze kadar yapılan birçok bilimsel çalışmaya göre makro fungusların antiviral, antibakteriyal, antiprotozoal ve antifungalözellik gösteren çeşitli kimyasal bileşiklere sahip olduğu bilinmektedir. Bu çalışmada Kırşehir ili ve ilçelerinden toplanan Kuzu Göbeği mantarlarının 18S rRNA ve ITS gen bölgeleri çoğaltılarak moleküler olarak tanımlamaları gerçekleştirilmiştir. Ayrıca, bu mantarlarının etanol, metanol ve sulu ekstraklarının antimikrobiyal etkileri çeşitli mikroorganizmalar üzerinde agar kuyucuk yöntemi ile araştırılmıştır. Son olarak, etkili bulunan ekstraktların Minimal İnhibisyon Konsantrasyon (MIC, MIK) değerleri Broth Dilüsyon metodu kullanılarak belirlenmiştir. Yapılan çalışmalar sonucunda en etkili ekstraktın etanol ekstraktı olduğu ve Pseudomonas aeruginosa ATCC43288 üzerine oldukça etkili olduğu tespit edilmiştir. Aynı zamanda etanol ve metanol ekstraktlarının Candida albicans ATCC60193 üzerinde de etkili olduğubelirlenmiştir. Elde edilen verilere göre, Kırşehir ilinden toplanan Morchella esculenta mantarının etanol ve metanol ekstraktlarının gram pozitif, gram negatif ve mayalara karşı etkili olduğu tespit edilmiştir. Sonuç olarak, M. esculenta mantarının antibakteriyel ve antifungal potansiyeli bakımından dikkate değer olduğu görülmektedir.

Keywords

Antimikrobiyal Aktivite Kuzu Göbeği Moleküler Karakterizasyon Morchella esculenta (L.) Pers

Supporting Institution

Kırşehir Ahi Evran Üniversitesi, Bilimsel Araştırma Projeleri Birimi

Project Number

FEF.A4.19.007

References

  • Acar, İ. ve Uzun, Y. (2017). An Interesting Half-Free Morel Record for Turkish Mycobiota (Morchella po-puliphila M. Kuo, M.C. Carter & J.D. Moore). The Journal of Fungus, 8(2), 125-8. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/357091
  • Acay, H. (2018). Yenilebilen Yabani Mantar Morchella esculenta (L.) Pers.’nın Besinsel Kalitesi ve Biyoak-tif Özelliklerinin Değerlendirilmesi. The Journal of Fungus, 9(2), 95-105. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/560149
  • Ajmal, M., Akram, A., Ara, A., Akhund, S. ve Nayyar, B. G. (2015). Morchella esculenta: An edible and health beneficial mushroom. Pakistan Journal of Food Science, 25(2), 71-8.
  • Baati, T., Horcajada, P., Gref, R., Couvreur, P. Ve Serre, C. (2011). Quantification of fumaric acid in liver, spleen and urine by high-performance liquid chromatography coupled to photodiode-array detection. Journal of Pharmaceutical and Biomedical Analysis, 56(4), 758-762. https://doi.org/10.1016/j.jpba.2011.07.011
  • Bekçi, H., Altınsoy, B., Sarıkaya, S., Onbaşılı, D. ve Yuvalı Çelik, G. (2011). Kastamonu Yöresinden Topla-nan Bazı Makrofungusların Antimikrobiyal Aktivitesi. Kastamonu Üniversitesi Orman Fakültesi Der-gisi, 11(2), 187-190. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/159627
  • Canlı, K., Benek, A., Şenturan, M., Akata, I. ve Altuner, E. M. (2019). In vitro Antimicrobial Activity of Morchella esculenta and Trametes versicolor. The Journal of Fungus, Aralık10(Özel Sayı), 28-33. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/900186
  • Duncan, C., Pugh, N., Pasco, D. S. ve Ross, S.A. (2002). Isolation of a galactomannan that enhances mac-rophage activation from the edible fungus Morchella esculenta. Journal of Agricultural and Food Chemistry, 50(20), 5683-5. https://doi.org/10.1021/jf020267c
  • Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium, 41, 95-8. Erişim adresi: https://www.academia.edu/2034992
  • Halliwell, B. (2011). Free radicals and antioxidants – quo vadis? Trends in Pharmacological Sciences, 32(3), 125-130. https://doi.org/10.1016/j.tips.2010.12.002
  • Halliwell, B. (2012). Free radicals and antioxidants: updating a personal view. Nutrition Reviews, 70(5), 257-265. https://doi.org/10.1111/j.1753-4887.2012.00476.x
  • Hamayun, M., Khan, M. A. ve Begum, S. (2003). Marketing of medicinal plants of Utror-Gabral Valleys, Swat, Pakistan. Journal of Ethnobotanical Leaflets, 2003(1), 13-22. Erişim adresi: https://www.researchgate.net/publication/38285163
  • Heleno, S.A., Stojkovic, D., Barros, L., Glamoclija, J., Sokovic, M., Martins, A., Queiroz, M. J. R. P. Ve Ferreira, I. C. F. R. (2013). A comparative study of chemical composition, antioxidant and antimicro-bial properties of Morchella esculenta (L.) Pers. from Portugal and Serbia. Food Research Internatio-nal, 51(1), 236-243. https://doi.org/10.1016/j.foodres.2012.12.020
  • Kalyoncu, F., Oskay, M. ve Kalmış, E. (2010). Bazı Yabani Makrofungus Misellerinin Antimikrobiyal Akti-vitelerinin Belirlenmesi. The Journal of Fungus, 1(1), 1-8. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/181923
  • Lindequist, U., Niedermeyer, T. H. J. ve Jülich, W. D. (2005). The pharmacological potential of mushrooms. Evidence-Based Complementary and Alternative Medicine, 2(3), 285–299. https://doi.org/10.1093/ecam/neh107
  • Magaldi, S., Essayag, S. M., Hartung de Capriles, C., Perez, C., Colella, M. T., Olaizola, C. ve Ontiveros, Y. (2004). Well diffusion for antifungal susceptibility testing. International Journal of Infectious Diseases, 8(1), 39-45. https://doi.org/10.1016/j.ijid.2003.03.002
  • Meng, F., Zhou, B., Lin, R., Jia, L., Liu, X., Deng, P., Fan, K., Wang, G., Wang, L. ve Zhang, J. (2010). Extraction optimization and in vivo antioxidant activities of exopolysaccharide by Morchella esculenta SO-01. Bioresource Technology, 101(12), 4564-9. https://doi.org/10.1016/j.biortech.2010.01.113
  • Roman, M. P. G., Mantilla, N. B., Florez, S. A. C., De Mandal, S., Passari, A. K., Ruiz-Villafan, B. ve Sanchez, S. (2020). Antimicrobial and Antioxidant Potential of Wild Edible Mushrooms. In An Intro-duction to Mushroom. IntechOpen; pp:1-18. Erişim adresi: https://www.intechopen.com/books/an-introduction-to-mushroom/antimicrobial-and-antioxidant-potential-of-wild-edible-mushrooms
  • Sayers, E. W., Cavanaugh, M., Clark, K., Ostell, J., Pruitt, K. D. ve Karsch-Mizrachi, I. (2020). GenBank. Nucleic Acids Research, 48, D84–D86. https://doi.org/10.1093/nar/gkz956
  • Shameem, N., Kamili, N. A., Ahmad, M., Masoodi, F.A. ve Parray, J.A. (2017). Antimicrobial activity of crude fractions and morel compounds from wild edible mushrooms of North western Himalaya. Mic-robial Pathogenesis, 105, 356-360. https://doi.org/10.1016/j.micpath.2017.03.005
  • Taşkın, H. ve Büyükalaca, S. (2012). Kuzu Göbeği (Morchella) Mantarı. Bahçe, 41(1), 25-36. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/39699
  • Venturini, M. E., Rivera, C. Z., Gonzales, C. ve Blanco, D. (2008). Antimicrobial activity of extracts of edib-le wild and cultivated mushrooms against foodborne bacterial strains. Journal of Food Protection, 71(8), 1701-6. https://doi.org/10.4315/0362-028X-71.8.1701
  • White, T.J., Bruns, T., Lee, S. ve Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications. Academic Press Inc., San Diego. Erişim adresi: https://www.researchgate.net/publication/262687766

Molecular Characterization And Determination Of Antimicrobial Activities Of Morchella Esculenta (L.) Pers (Lamb Belly) Collected From Kirşehir

Year 2021, Volume: 7 Issue: 1, 48 - 59, 20.03.2021

Elif Sevim , Ali Sevim

https://doi.org/10.28979/jarnas.890330
Cited By: 1

Abstract

It has been known for a long time that natural edible mushrooms have been used traditionally as food and medicine in many countries of the world. Morchella esculenta (L.) Pers (belly) is extremely valuable in terms of medicinal use and nutritional value. According to many scientific studies performed to date, macro fungi are known to have various chemical compounds showing antiviral, antibacterial, antiprotozoal, and antifungal properties. In this study, lamb belly mushrooms collected from Kırşehir province and its districts were molecularly identified using amplification of 18S rRNA and ITS gene regions. In addition, the antimicrobial effects of ethanol, methanol and aqueous extracts of these mushrooms were investigated by using the agar well method against various microorgan-isms. Finally, Minimal Inhibition Concentration (MIC, MIK) values of the effective extracts were determined using the Broth Dilution method. As a result of the studies, it has been determined that the most effective extract is ethanol extract and it is highly effective against Pseudomonas aeruginosa ATCC43288. It has also been determined that ethanol and methanol extracts are effective against Candida albicans ATCC60193. According to the data obtained, it was determined that the ethanol and methanol extracts of Morchella esculenta mushrooms collected from Kırşehir province were effective against gram positive, gram negative and yeast. Consequently, M. esculenta mushroom appears to be notable for its antibacterial and antifungal potential.

Keywords

Antimicrobial Activity Lamb Belly Molecular Characterization Morchella esculenta (L.) Pers

Project Number

FEF.A4.19.007

References

  • Acar, İ. ve Uzun, Y. (2017). An Interesting Half-Free Morel Record for Turkish Mycobiota (Morchella po-puliphila M. Kuo, M.C. Carter & J.D. Moore). The Journal of Fungus, 8(2), 125-8. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/357091
  • Acay, H. (2018). Yenilebilen Yabani Mantar Morchella esculenta (L.) Pers.’nın Besinsel Kalitesi ve Biyoak-tif Özelliklerinin Değerlendirilmesi. The Journal of Fungus, 9(2), 95-105. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/560149
  • Ajmal, M., Akram, A., Ara, A., Akhund, S. ve Nayyar, B. G. (2015). Morchella esculenta: An edible and health beneficial mushroom. Pakistan Journal of Food Science, 25(2), 71-8.
  • Baati, T., Horcajada, P., Gref, R., Couvreur, P. Ve Serre, C. (2011). Quantification of fumaric acid in liver, spleen and urine by high-performance liquid chromatography coupled to photodiode-array detection. Journal of Pharmaceutical and Biomedical Analysis, 56(4), 758-762. https://doi.org/10.1016/j.jpba.2011.07.011
  • Bekçi, H., Altınsoy, B., Sarıkaya, S., Onbaşılı, D. ve Yuvalı Çelik, G. (2011). Kastamonu Yöresinden Topla-nan Bazı Makrofungusların Antimikrobiyal Aktivitesi. Kastamonu Üniversitesi Orman Fakültesi Der-gisi, 11(2), 187-190. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/159627
  • Canlı, K., Benek, A., Şenturan, M., Akata, I. ve Altuner, E. M. (2019). In vitro Antimicrobial Activity of Morchella esculenta and Trametes versicolor. The Journal of Fungus, Aralık10(Özel Sayı), 28-33. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/900186
  • Duncan, C., Pugh, N., Pasco, D. S. ve Ross, S.A. (2002). Isolation of a galactomannan that enhances mac-rophage activation from the edible fungus Morchella esculenta. Journal of Agricultural and Food Chemistry, 50(20), 5683-5. https://doi.org/10.1021/jf020267c
  • Hall, T.A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium, 41, 95-8. Erişim adresi: https://www.academia.edu/2034992
  • Halliwell, B. (2011). Free radicals and antioxidants – quo vadis? Trends in Pharmacological Sciences, 32(3), 125-130. https://doi.org/10.1016/j.tips.2010.12.002
  • Halliwell, B. (2012). Free radicals and antioxidants: updating a personal view. Nutrition Reviews, 70(5), 257-265. https://doi.org/10.1111/j.1753-4887.2012.00476.x
  • Hamayun, M., Khan, M. A. ve Begum, S. (2003). Marketing of medicinal plants of Utror-Gabral Valleys, Swat, Pakistan. Journal of Ethnobotanical Leaflets, 2003(1), 13-22. Erişim adresi: https://www.researchgate.net/publication/38285163
  • Heleno, S.A., Stojkovic, D., Barros, L., Glamoclija, J., Sokovic, M., Martins, A., Queiroz, M. J. R. P. Ve Ferreira, I. C. F. R. (2013). A comparative study of chemical composition, antioxidant and antimicro-bial properties of Morchella esculenta (L.) Pers. from Portugal and Serbia. Food Research Internatio-nal, 51(1), 236-243. https://doi.org/10.1016/j.foodres.2012.12.020
  • Kalyoncu, F., Oskay, M. ve Kalmış, E. (2010). Bazı Yabani Makrofungus Misellerinin Antimikrobiyal Akti-vitelerinin Belirlenmesi. The Journal of Fungus, 1(1), 1-8. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/181923
  • Lindequist, U., Niedermeyer, T. H. J. ve Jülich, W. D. (2005). The pharmacological potential of mushrooms. Evidence-Based Complementary and Alternative Medicine, 2(3), 285–299. https://doi.org/10.1093/ecam/neh107
  • Magaldi, S., Essayag, S. M., Hartung de Capriles, C., Perez, C., Colella, M. T., Olaizola, C. ve Ontiveros, Y. (2004). Well diffusion for antifungal susceptibility testing. International Journal of Infectious Diseases, 8(1), 39-45. https://doi.org/10.1016/j.ijid.2003.03.002
  • Meng, F., Zhou, B., Lin, R., Jia, L., Liu, X., Deng, P., Fan, K., Wang, G., Wang, L. ve Zhang, J. (2010). Extraction optimization and in vivo antioxidant activities of exopolysaccharide by Morchella esculenta SO-01. Bioresource Technology, 101(12), 4564-9. https://doi.org/10.1016/j.biortech.2010.01.113
  • Roman, M. P. G., Mantilla, N. B., Florez, S. A. C., De Mandal, S., Passari, A. K., Ruiz-Villafan, B. ve Sanchez, S. (2020). Antimicrobial and Antioxidant Potential of Wild Edible Mushrooms. In An Intro-duction to Mushroom. IntechOpen; pp:1-18. Erişim adresi: https://www.intechopen.com/books/an-introduction-to-mushroom/antimicrobial-and-antioxidant-potential-of-wild-edible-mushrooms
  • Sayers, E. W., Cavanaugh, M., Clark, K., Ostell, J., Pruitt, K. D. ve Karsch-Mizrachi, I. (2020). GenBank. Nucleic Acids Research, 48, D84–D86. https://doi.org/10.1093/nar/gkz956
  • Shameem, N., Kamili, N. A., Ahmad, M., Masoodi, F.A. ve Parray, J.A. (2017). Antimicrobial activity of crude fractions and morel compounds from wild edible mushrooms of North western Himalaya. Mic-robial Pathogenesis, 105, 356-360. https://doi.org/10.1016/j.micpath.2017.03.005
  • Taşkın, H. ve Büyükalaca, S. (2012). Kuzu Göbeği (Morchella) Mantarı. Bahçe, 41(1), 25-36. Erişim adresi: https://dergipark.org.tr/tr/download/article-file/39699
  • Venturini, M. E., Rivera, C. Z., Gonzales, C. ve Blanco, D. (2008). Antimicrobial activity of extracts of edib-le wild and cultivated mushrooms against foodborne bacterial strains. Journal of Food Protection, 71(8), 1701-6. https://doi.org/10.4315/0362-028X-71.8.1701
  • White, T.J., Bruns, T., Lee, S. ve Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications. Academic Press Inc., San Diego. Erişim adresi: https://www.researchgate.net/publication/262687766
There are 22 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Elif Sevim 0000-0002-6455-1333

Ali Sevim 0000-0003-2472-599X

Project Number FEF.A4.19.007
Publication Date March 20, 2021
Submission Date October 10, 2020
Published in Issue Year 2021 Volume: 7 Issue: 1

Cite

APA Sevim, E., & Sevim, A. (2021). Kırşehir İlinden Toplanan Morchella esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu ve Antimikrobiyal Aktivitelerinin Belirlenmesi. Journal of Advanced Research in Natural and Applied Sciences, 7(1), 48-59. https://doi.org/10.28979/jarnas.890330
AMA Sevim E, Sevim A. Kırşehir İlinden Toplanan Morchella esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu ve Antimikrobiyal Aktivitelerinin Belirlenmesi. JARNAS. March 2021;7(1):48-59. doi:10.28979/jarnas.890330
Chicago Sevim, Elif, and Ali Sevim. “Kırşehir İlinden Toplanan Morchella Esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu Ve Antimikrobiyal Aktivitelerinin Belirlenmesi”. Journal of Advanced Research in Natural and Applied Sciences 7, no. 1 (March 2021): 48-59. https://doi.org/10.28979/jarnas.890330.
EndNote Sevim E, Sevim A (March 1, 2021) Kırşehir İlinden Toplanan Morchella esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu ve Antimikrobiyal Aktivitelerinin Belirlenmesi. Journal of Advanced Research in Natural and Applied Sciences 7 1 48–59.
IEEE E. Sevim and A. Sevim, “Kırşehir İlinden Toplanan Morchella esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu ve Antimikrobiyal Aktivitelerinin Belirlenmesi”, JARNAS, vol. 7, no. 1, pp. 48–59, 2021, doi: 10.28979/jarnas.890330.
ISNAD Sevim, Elif - Sevim, Ali. “Kırşehir İlinden Toplanan Morchella Esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu Ve Antimikrobiyal Aktivitelerinin Belirlenmesi”. Journal of Advanced Research in Natural and Applied Sciences 7/1 (March 2021), 48-59. https://doi.org/10.28979/jarnas.890330.
JAMA Sevim E, Sevim A. Kırşehir İlinden Toplanan Morchella esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu ve Antimikrobiyal Aktivitelerinin Belirlenmesi. JARNAS. 2021;7:48–59.
MLA Sevim, Elif and Ali Sevim. “Kırşehir İlinden Toplanan Morchella Esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu Ve Antimikrobiyal Aktivitelerinin Belirlenmesi”. Journal of Advanced Research in Natural and Applied Sciences, vol. 7, no. 1, 2021, pp. 48-59, doi:10.28979/jarnas.890330.
Vancouver Sevim E, Sevim A. Kırşehir İlinden Toplanan Morchella esculenta (L.) Pers (Kuzu Göbeği) Mantarlarının Moleküler Karakterizasyonu ve Antimikrobiyal Aktivitelerinin Belirlenmesi. JARNAS. 2021;7(1):48-59.

Cited By

Determination of Antioxidant and Antimicrobial Effects of Trametes versicolor (L.) Lloyd Extracts in Different Solvents
Turkish Journal of Science and Technology
https://doi.org/10.55525/tjst.1076245
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