The Application of Lipoic Acid in Experimentally Induced Tympanosclerosis in Rats

Year 2020, Volume: 10 Issue: 1, 70 - 74, 31.03.2020

Fatih Turan , İlyas Sayar , İsmail Salcan

https://doi.org/10.16899/jcm.634978

Abstract

Abstract

Background/Aims:Tympanosclerosis
is an abnormal tissue reaction resulting in sclerotic and hyalinization changes
in the tympanic membrane and middle ear. 
We hypothesized that alpha lipoic acid, an antioxidant, may represent a
simple and effective form of treatment in inflammattion-related
tympanosclerosis. The purpose of the study was to investigate the effects
of alpha lipoic acid on experimentally induced inflammattion-related tympanosclerosis
in rats.

Methods:Twenty
albino Wistar rats were randomly divided into two equal groups. The first
(study) group was administered 50 mg/kg alpha lipoic acid by the intraperitoneal
route once daily for 7 days. The second group was administered the same saline
solution intraperitoneally once daily for 7 days. Inflammation, fibrosis and
lamina propria thickness were assessed at the subsequent histopathological
examination.

Results:In
the study group, significantly less middle ear inflammation and fibrosis
developed and the tympanic membrane lamina propria was thinner compared to the control
group. 

Conclusions: Alpha
lipoic acid was found capable of preventing experimentally induced inflammattion-related
tympanosclerosis with its antioxidant characteristics.

Keywords

alpha lipoic acid, inflammation, fibrosis, fibrosis

Supporting Institution

Erzincan University Committee for Scientific Research Projects.

Project Number

SAG-A-140613-0033

Ratlarda Deneyse Olarak Oluşturulan İnfilamasyonla İlişkili Timpanoskleroza Alfa Lipoik Asidin Etkisi

Abstract

Amaç: Timpanoskleroz timpanik membran ve orta kulak mukozasının sklerotik ve hiyalinize değişikliği ile giden anormal bir doku reaksiyonudur. Bir antioksidan olan alfa lipoik asidin, inflamasyonla ile ilişkili timpanosklerozda basit ve etkili bir tedavi biçimini temsil edebileceğini varsaydık. Çalışmanın amacı, alfa lipoik asidin sıçanlarda deneysel olarak indüklenen infilamasyon ile ilşkili timpanoskleroz üzerindeki etkilerini araştırmaktı.
Materyal Metod: Çalışmamızda 20 adet Wistar albino sıçan kullanıldı. Sıçanlar rastgele olarak onarlı iki gruba ayrıldı. 1. gruba ALA 50 mg/kg yedi gün boyunca günde bir defa intraperitoneal uygulandı. 2. gruba salin aynı miktarda yedi gün boyunca günde bir defa intraperitoneal uygulandı. Histopatolojik olarak değerlendirmemizde inflamasyon, fibrozis ve lamina propria kalınlığı değerlendirildi.
Bulgular: Orta kulakta enflamasyon, fibrozis ve timpanik membran lamina propria kalınlığı değerlendirildiğinde, çalışma grubunda kontrol gurubuna göre daha az olduğu değerlendirildi.
Sonuç: Antiokstidan özellik gösteren alfa lipoik asit’in deneysel olarak oluşturulmuş inflamasyonla ile ilişkili timpanosklerozu önleyebileceği görüldü.

Keywords

Alfa-Lipoik asit, inflamasyon, fibroz

Project Number

SAG-A-140613-0033

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