Levels of leukocyte oxidative DNA damage (8-OHdG), serum coenzyme Q10 and lipid peroxidation in the formation attacks of patients with multiple sclerosis

Year 2017, , 601 - 607, 12.08.2017

Erdem Çokluk Aysel Milanlıoğlu Zübeyir Huyut , Vedat Çilingir Hamit Hakan Alp , Mehmet Nuri Aydın Mehmet Ramazan Şekeroğlu , Ragıp Balahoroğlu

https://doi.org/10.37212/jcnos.334360

Cited By: 1

Abstract

Multiple sclerosis (MS) is a demyelinating disease
of the nervous system. Evidence about oxidative stress plays an important role
in the pathogenesis of MS is increasing day by day. In our study, we aimed to
investigate the effect of oxidative DNA damage and oxidative stress in the
pathogenesis of MS disease. Blood samples were obtained from during an attack
(Group 1), between attacks (Group 2) of MS patients (20 male and 10 female) and
30 healthy volunteers (Group 3). Malondialdehyde (MDA) levels as indicator of
oxidized lipids were detected using fluorescence dedector with high pressure
liquid chromatograph (HPLC). DNA was extracted from leukocytes of control and
patients with MS and then we measured 8-hydroxy- 2' -deoxyguanosine (8-OHdG) and deoxyguanosin (dG) by using HPLC method with electrochemical and
UV detector, respectively. Measurement of oxidized coenzyme Q10 (CoQ10) and
reduced CoQ (CoQ10H) was performed by using UV detector with HPLC method. Serum
MDA level of group 1 was significantly higher than those in group 2 and group 3
(p< 0.001). 8- OHdG/106 dG ratio
of group 1 was significantly higher than those in group 2 and group 3 (p<
0.001). CoQ10/CoQ10H rates of group 1 were significantly increased compared
with group 2 and group 3 (p<0.001). In conclusion, we observed that
oxidative DNA damage, lipid and mitochondria oxidative damage were high in
blood of patients with MS. It seems that oxidative stress acts a play role the
pathogenesis of MS patients as well as induces attacks.

Keywords

Coenzyme Q10; Lipid peroxidation; Oxidative DNA damage; Multiple sclerosis

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