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Year 2018, , 778 - 778, 18.08.2018

Denis Rousseau

https://doi.org/10.37212/jcnos.609964

Abstract

References

  • Li S, Bouzar C, Cottet-Rousselle C, Zagotta I, Lamarche F, Wabitsch M, Tokarska-Schlattner M, Fischer-Posovszky P, Schlattner U, Rousseau D.Biochim Biophys Acta. 2016 Jun;1857(6):643-52. doi: 10.1016/j.bbabio.2016.03.009. Epub 2016 Mar 8.

Western-blot, PCR and immunofluorescence analysis in mitochondrial biogenesis studies

Year 2018, , 778 - 778, 18.08.2018

Denis Rousseau

https://doi.org/10.37212/jcnos.609964

Abstract

Mitochondria are providing an essential amount of energy to the cell, to achieve in homeostasis, metabolic increases, proliferation and differentiation processes. Also, mitochondrial deficiencies have severe or lethal impacts on cell viability. Among the 3000 proteins involved in mitochondrial activities, ATAD3 is a major one as essential for mitochondrial biogenesis, vital as early as embryonic implantation. In order to see its  impact at animal level, we have used ATAD3+/- mice to investigate its role in running training and in high calorie diet. We found here that ATAD3 expression level avoids running capacity improvement and has a strong effect on weight increase, underlying its important role in mitochondrial mass regulations. Prior to this presentation we will emphasize on the potential of Western-blot, PCR and immunofluorescence analysis in biomedical researches.

Keywords

Mitochondria ATAD3 Western-blot PCR immunofluorescence

References

  • Li S, Bouzar C, Cottet-Rousselle C, Zagotta I, Lamarche F, Wabitsch M, Tokarska-Schlattner M, Fischer-Posovszky P, Schlattner U, Rousseau D.Biochim Biophys Acta. 2016 Jun;1857(6):643-52. doi: 10.1016/j.bbabio.2016.03.009. Epub 2016 Mar 8.
There are 1 citations in total.

Details

Primary Language English
Subjects Clinical Sciences
Journal Section Original Articles
Authors

Denis Rousseau This is me

Publication Date August 18, 2018
Published in Issue Year 2018

Cite

APA Rousseau, D. (2018). Western-blot, PCR and immunofluorescence analysis in mitochondrial biogenesis studies. Journal of Cellular Neuroscience and Oxidative Stress, 10(3), 778-778. https://doi.org/10.37212/jcnos.609964
AMA Rousseau D. Western-blot, PCR and immunofluorescence analysis in mitochondrial biogenesis studies. J Cell Neurosci Oxid Stress. August 2018;10(3):778-778. doi:10.37212/jcnos.609964
Chicago Rousseau, Denis. “Western-Blot, PCR and Immunofluorescence Analysis in Mitochondrial Biogenesis Studies”. Journal of Cellular Neuroscience and Oxidative Stress 10, no. 3 (August 2018): 778-78. https://doi.org/10.37212/jcnos.609964.
EndNote Rousseau D (August 1, 2018) Western-blot, PCR and immunofluorescence analysis in mitochondrial biogenesis studies. Journal of Cellular Neuroscience and Oxidative Stress 10 3 778–778.
IEEE D. Rousseau, “Western-blot, PCR and immunofluorescence analysis in mitochondrial biogenesis studies”, J Cell Neurosci Oxid Stress, vol. 10, no. 3, pp. 778–778, 2018, doi: 10.37212/jcnos.609964.
ISNAD Rousseau, Denis. “Western-Blot, PCR and Immunofluorescence Analysis in Mitochondrial Biogenesis Studies”. Journal of Cellular Neuroscience and Oxidative Stress 10/3 (August 2018), 778-778. https://doi.org/10.37212/jcnos.609964.
JAMA Rousseau D. Western-blot, PCR and immunofluorescence analysis in mitochondrial biogenesis studies. J Cell Neurosci Oxid Stress. 2018;10:778–778.
MLA Rousseau, Denis. “Western-Blot, PCR and Immunofluorescence Analysis in Mitochondrial Biogenesis Studies”. Journal of Cellular Neuroscience and Oxidative Stress, vol. 10, no. 3, 2018, pp. 778-, doi:10.37212/jcnos.609964.
Vancouver Rousseau D. Western-blot, PCR and immunofluorescence analysis in mitochondrial biogenesis studies. J Cell Neurosci Oxid Stress. 2018;10(3):778-.