Effects of alpha lipoic acid on TRPV1 cation channel in dorsal root ganglion of diabetes-induced rats

Year 2018, , 796 - 796, 18.08.2018

Yener Yazğan

https://doi.org/10.37212/jcnos.610159

Abstract

Diabetes is a common chronic metabolic disease worldwide. It causes diabetes, tissue degenerations, cell death and associated functional disability. Diabetes also causes the development of common diseases such as cardiovascular and nervous diseases. The most important pathophysiological mechanisms that are accepted in describing diabetic degenerative processes are oxidative stress damage (El-Refaei et al. 2014). Neuropathic pain is induced by several factors including inflammation, excessive Ca2+ influx, oxidative stress and tissue degeneration. Inflammatory reactions in destroying neurons lead to the activation of the pain molecular pathway. In addition, destruction cell membranes induce excessive Ca2+ entry in the neurons, because the Ca2+ concentration is about 10.000 times higher in out of the neurons than in the inside of the neuron. Overload Ca2+ influx into cytosol and it leads to excessive production of ROS in the neurons. Alpha lipoic acid (ALA) is an important member of the thiol cycle because it is containing the sulfur groups. It is powerful antioxidant substance and it can prevent the complications of diabetes (Ghibu et al. 2009). Transient receptor potential (TRP) channels have six subfamilies and 28 members in human. Most of these channels are responsible in dorsal root ganglia (DRG) neurons for the Ca2+ permeation especially in neuronal cells. Expression level of the TRPV1 channels is high in the DRG neurons and they show oxidative stress dependent activation. The TRPV1 channel expression levels in the DRG increased in different types of pain. Forty female rats were divided into four groups: First group was used as control. Second group used as diabetic. Third and fourth groups received ALA and STZ+ALA, respectively. Diabetes was induced using a single dose of intraperitoneal STZ. On 14th day of DRG samples were freshly taken from all animals. In plate reader analyses, we observed modulator role of ALA on apoptosis, caspase 3, caspase 9, mitochondrial depolarization and cytosolic ROS production values on TRPV1 channel in the DRG neurons. In addition, we observed modulator role of ALA on intracellular Ca2+ concentration through inhibition of TRPV1 in neuropathic pain-induced rats. In conclusion, in our diabetes experimental model, oxidative stress are involved in the Ca2+ entry-induced neuronal death, and modulation of this channel activity by ALA pretreatment may account for their neuroprotective activity against apoptosis.

Keywords

Apoptosis, Oxidative stress, Alpha lipoic acid, Diabetes

References

• El-Refaei MF, Abduljawad SH, Alghamdi AH. 2014. Alternative medicine in diabetes - Role of angiogenesis, oxidative stress, and chronic inflammation. Rev Diabet Stud. Fall- Winter;11:231-44.
• Ghibu S, Richard C, Vergely C, Zeller M, Cottin Y, Rochette L. 2009. Antioxidant properties of an endogenous thiol: Alpha-lipoic acid, useful in the prevention of cardiovascular diseases. J Cardiovasc Pharmacol. 54:391-8.