Effects of Different Doses of Curcumin on Apoptosis, Mitochondrial Oxidative Stress and Calcium Ion Influx in DBRG Glioblastoma Cells

Year 2017, Volume: 9 Issue: 2, 617 - 629, 30.08.2017

Ahmi Öz , Ömer Çelik , İshak Suat Övey

https://doi.org/10.37212/jcnos.330858

Cited By: 14

Abstract

Transient Receptor
Potential (TRP) channels superfamily has mostly calcium ion (Ca²⁺)
permeable non-selective cation channels. Transient receptor potential
melastatin subfamily 2 (TRPM2) is widely expressed in central nervous system. Intracellular
Ca²⁺ concentration ([Ca²⁺]_i) may change depend
on TRPM2 and TRPM8 activations from extracellular liquid to cytosol. Curcumin
as natural antioxidant shows phenolic structure, synthesized by Curcuma longa L. (Indian saffron, turmeric),
has powerful non-enzymatically antioxidant effects. Low dose curcumin treatment
can decrease calcium signaling via
TRPM2 channel inhibition and prevent elevation of [Ca²⁺]_i levels.
Hence, we investigated effects of four different concentrations (5, 10, 25
and 50 µM) of curcumin on apoptosis and cell viability (MTT), reactive oxygen
species (ROS) production, mitochondrial membrane potential levels, caspase 3
and caspase 9 values in DBRG glioblastoma cells.

            We
found that curcumin reduces cell viability by concentration dependent manner.
It was also observed that curcumin induces apoptosis via caspase 3 and 9
related pathways. However, it was not found any direct relationship between the
effect of increased concentrations of curcumin and inhibition or activation of TRPM2
mediated Ca²⁺ signaling in the DBTRG cells. The [Ca²⁺]_i
concentration was lower in 5 µM group as compare to control group. Curcumin acted
important role on decrease of mitochondrial membrane potential and
intracellular ROS production in the cells. Moreover, curcumin treatment
markedly supported GSH concentration levels in the cells. 

In conclusion, it was firstly assessed the effects of different
doses of curcumin on TRPM2 mediated calcium signaling and interaction with
various apoptosis parameters in DBTRG glioblastoma cells.

Keywords

Curcumin, cell viability, Ca2+ signaling, TRPM2, DBTRG cells

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