The effects of CAPE on oxidative stress and histopathological values in rats treated with subacute dichlorvos

Year 2018, Volume: 10 Issue: 1, 647 - 655, 26.04.2018

Gökhan Nur , Aysel Güven , Pınar Aksu Kılıçle

https://doi.org/10.37212/jcnos.418660

Cited By: 2

Abstract

This study was designed to investigate the protective effect of CAPE against dichlorvos induced hepatotoxicity in rats. 40 rats were used in the study, including 10 in each group. Corn oil of 5 mg/kg (solvent) was administered intragastrically to rats in the group 1, dichlorvos of 5 mg/kg was dissolved in corn oil and administered to rats in the group 2 via gavage, dichlorvos (5 mg/kg, via gavage route) and CAPE (10 μM/kg, intraperitoneally) were administered to rats of the group 3 with one-hour interval providing that CAPE treatment was started three days earlier than dichlorvos administration. The rats in the group 4 were administered with 10% solvent ethanol via intraperitoneally. All administrations continued for 15 days and blood of animals was drawn via intracardiac route after they were sacrificed by cervical dislocation under ketamine/xylazine anesthesia. Total antioxidant, total oxidant, and paraoxonase levels were measured from the obtained plasma samples. As a result of dichlorvos administration, paraoxonase activity and total antioxidant levels decreased; whereas, total oxidant levels increased. Histopathological analysis revealed that liver tissue appeared normal in control groups (1 and 4); on the other hand, there were degeneration, congestion, cellular infiltration, and necrotic areas in the group administered with dichlorvos. Even though frequency of lesions decreased, similar lesions were observed in the group with Dichlorvos+CAPE. Consequently, it was determined that while dichlorvos administration increased oxidative stress, CAPE administration had a protective potential increasing antioxidant capacity.

Keywords

Dichlorvos; CAPE; Hepatotoxicity; Oxidative stress; Subacute administration.

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