Antioxidative and Neurotherapeutic Effect of Ascorbic acid on Albino rats Induced with Traumatic Brain Injury

Year 2020, Volume: 12 Issue: 1, 922 - 936, 12.10.2020

Ibrahim Bulama , Nasiru Suleiman , Lawal Bilbis , Abdullahi Abbas , Nasiru Jinjiri , Yusuf Saidu , Mohamad Aris Mohd Moklas , Che Norma Mat Taib , Musa Chiroma

https://doi.org/10.37212/jcnos.800233

Abstract

Generation of reactive oxygen species (ROS) during traumatic brain injury (TBI) has been identified as an important factor that is responsible for disease progression and cell death, particularly in secondary injury process. Ascorbic acid (AA) is an exogenous antioxidant that can be used to quench ROS in neurodegeneration. Its antioxidant properties have been reported in some neurodegenerative conditions in rats. In the present study, we examined the neurotherapeutic effects of AA in TBI induced rats. Three groups of seven rats each were used for this study. Group I was induced with TBI and treated with AA (67.5 mg/kg orally), group II was traumatized but not treated (TNT) while group III was neither traumatized nor treated (NTNT). Treatment started 30 min after TBI and lasted for 21 days. Morris water maze (MWM), Elevated plus maze, and Open field test were carried out. Antioxidant enzymes [(Superoxide dismutase (SOD), Catalase (CAT) Glutathion peroxidase (GPx)] and their gene expression were analyzed. Malondialdehyde level, S100B concentration and histological studies were conducted. The treatment with AA improved learning and memory, locomotor function and decreased anxiety in the treated groups compared to group II. S100B was significantly (p<0.05) lowered in the treated group compared to the group II rats. Treatment with AA also decreases malondialdehyde level when compared to group II. There were increased SOD, CAT and GPx activities in the treated group when compared to the control group. These were in agreement with their gene expressions that are highly expressed in the same groups. Our results suggest that neuroprotective effects of AA in albino rats can be the result of reduced lipid peroxidation and enhanced antioxidant status which can be a good neurotherapeutic strategy.

Keywords

Ascorbic acid, , Neurotherapeutic, antioxidant, Neurological, Neurodegeneration, antioxidant,

References

• Angeloni, C., Prata, C. Vieceli, F. Sega, D., Piperno, R. and Hrelia, S. 2015. Traumatic Brain Injury and NADPH Oxidase: A Deep Relationship. Oxidative Medicine and Cellular Longevity, 2015: 1-10
• Andre, M. A., Souza, L., Walz, R., and Dafre, A. 2014. Perspective on Molecular Biomarker of Oxidative Stress and Antioxidant Strategies in Traumatic Brain Injury. Biomedical. Research. International, 2014: 1-18.
• Bulama I., Bilbis, LS., Abbas, AY., Saidu, Y., Suleiman, N., Ibrahim, K. Cognitive impairment and the role of vitamin C, Dimethylsulfoxide and Alpha lipoic acid during Traumatic Brain Injury in Albino rats 2017. Journal of neurobehavioral sciences, 4(1) Pp.1-12
• Calero, C.I.; Vickers, E.; Cid, G.M.; Aguayo, L.G.; von Gersdorff, H.; Calvo, D.J.2011 Allosteric modulation of retinal GABA receptors by ascorbic acid. J. Neurosci., 31, 9672–9682
• Chen, Y., Huang, EK., Kuo, T., Miller, J., Chiang, Y and Hoffer, BJ. 2017 Impact of Traumatic Brain Injury on Dopaminergic Transmission Cell Transplantation, 26 (7) 1156-1168
• Chomczynski, P. and Mackey, K. 1985. Short technical report: Modification of the TRIZOL reagent procedure for isolation of RNA from Polysaccharide-and proteoglycan-rich sources. Biotechniques; 19(6): 42-45
• de Oliveira, I.J.L., de Souza, V.V., Motta, V. and Da-Silva, S.L. 2015. Effects of Oral Vitamin C Supplementation on Anxiety in Students: A Double-Blind, Randomized, Placebo-Controlled Trial. Pakistan Journal of Biological Sciences, 18(1) 11-18
• Dadas, A., Washington, J., Diaz-Arrastia, R., Janigro, D.(2018) Biomarkers in traumatic brain injury (TBI): A review. Neuropsychiatric Disease and Treatment 2018:14 2989–3000 Devi, SA., Vani, R., Subramanyam, M.V., Reddy, SS., Jeevaratnam, K. 2007 Intermittent hypobaric hypoxia-induced oxidative stress in rat erythrocytes: protective effects of vitamin E, vitamin C, and carnitine Cell Biochemisrty Function 2007, vol. 25, no.1, Pp.221-231
• Dos Santos, L.O., Caldas, G.G., Santos, C.R.O. & Junior, D.B. 2018 Traumatic brain injury in dogs and cats: a systematic review Veterinarni Medicina, vol. 63, no.8, Pp.345–357
• Domith, I., Socodato, R., Portugal, CC., Munis, AF., Duarte‐Silva, AT. & Paes‐de‐Carvalho, R. Journal of neurochemistry, 2018, vol.144, no.4, Pp.408-20.
• Donat, CK., Scott, G., Gentleman, MS and Magdalena S. 2017 Microglial activation in Traumatic brain injury Frontiers in Aging Neuroscience 9(206)
• Engwa, G. A. 2018. Free Radicals and the Role of Plant Phytochemicals as Antioxidants Against Oxidative Stress-Related Diseases. In Phytochemicals: Source of Antioxidants and Role in Disease Prevention, Toshiki Asao and Md Asaduzzaman Eds. London: IntechOpen Limited, Pp.5772-76719.
• Fetoui H, Garoui EM, Makni-ayadi F, Zeghal N 2008 Oxidative stress induced by lambda-cyhalothrin (LTC) in rat erythrocytes and brain: attenuation by vitamin C. EnvironToxicol Phar 26:225–231
• Foda, M., & Marmarou , A. 1994. A new model of diffuse brain injury in rats part II: Morphological characterization. J Neurosurg, 80: Pp301-13.
• Figueroa-Méndez, R.., Rivas-Arancibia, S. 2015 Vitamin C in Health and Disease: Its Role in the Metabolism of Cells and Redox State in the Brain. Front. Physiol., 23, 397
• Guney, E. M.F., Ceylan, A., Tektas, M. A. and Ergin, M. et al., 2014. Oxidative stress in children and adolescents with anxiety disorders. J. Affect. Disorders, 156, 62-66
• Hansen, S.N.; Tveden-Nyborg, P.; Lykkesfeldt, J. Does vitamin C deficiency affect cognitive development and function? Nutrients 2014, 6, 3818–3846
• Hendoui, N., Beigmohammadi, M.T., Mahmoodpoor, A., Ahmadi, A., Abdollahi, M. Hasanpour, M., Hadi, F., Khazaeipour, Z., Mousavi, S. And Mojtahedzadeh, M. 2013 Reliability of calcium-binding protein S100B measurement toward optimization of hyperosmolal therapy in traumatic brain injury European Review for Medical and Pharmacological Sciences; 17: 477 – 485
• Hole, P. S. Darley, R. L. and Tonks, A. 2011“Do reactive oxygen species play a role in myeloid leukemias?” Blood, 117 (22) pp. 5816–5826,
• Kocot J, Luchowska-Kocot D, Kiełczykowska M, Musik I, and Kurzepa J. 2017. Does vitamin C influence neurodegenerative diseases and psychiatric disorders?. Nutrients, vol.9 no.7, Pp.659
• Koza, L. and Linseman, DA (2019).Glutathione precursors shield the brain from trauma. Neural Regen Res, 14 (10)1701-1702
• Lawal, N., Hair-Bejo, M., Arshad, SS., Omar, A. and Ideris, A. (2017) "Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line", Advances in Virology,(2017)Pp1-9. https://doi.org/10.1155/2017/8359047
• Lifshittz, J., Kelley, B.J. and Povlishock, J.T.(2007). Perisomatic thalamic axotomy after diffuse traumatic brain injury is associated with atrophy rather than cell death. Journal Neuropathology and Experimental Neurology, 66:218-29.
• Livak, K. J. and Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods, 25(4), 402-408.
• Marmarou, A. (1994). A new model of diffuse brain injury in rats part II: Morphological characterization. Journal of Neurosurgery, 80 : 301-13
• McAllister, TW..2011 Neurobiological consequences of traumatic brain injury. Dialogues in clinical neuroscience vol.13, no.3, P. 287
• Mefford, IN., Oke, AF., Adams, RN. 1981 Regional distribution of ascorbate in human brain Brain Research, vol.212, no.1, Pp.223–226
• Millby, K., Oke, A., and Adams, RN. 1982 Detailed mapping of ascorbate distribution in rat brain Neuroscince letter, vol.28, no.82, Pp.169-174
• Mori, T., Tan, J., Arendash, G.W., Koyama, N., Nojima, Y. and Town, T. 2008. Over expression of human S100B exacerbates brain damage and peri infarct gliosis after permanent focal ischemia. Stroke, 39, 2114-2121
• Morris, R. (1984). Development of a Water-Maze procedure for studying Spatial learning in the rat, Journal of Neuroscience. Methods, 11: 47-60
• Moutinho, M., Codocedo, JF., Puntambekar, SS., Landreth, GE. 2019 Nuclear Receptors as Therapeutic Targets for Neurodegenerative Diseases: Lost in Translation. pharmacology and toxicology. vol. 6, no.59, Pp. 237-61.
• Muller MB, Zimmermann S, Sillaber I, Hagemeyer TP, Deussing JM, Timpl P, Kormann MS, Droste SK, Kuhn R, Reul JM, Holsboer F, Wurst W 2003 Limbic corticotropin-releasing hormone receptor 1 mediates anxiety-related behavior and hormonal adaptation to stress. Nat Neurosci 6:1100-1107.
• Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G. and Erlich H. 1986 Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. Cold Spring Harbor symposia on quantitative biology 51: 263-273
• Olajide, O.J.; Yawson, E.O.; Gbadamosi, I.T.; Arogundade, T.T.; Lambe, E.; Obasi, K.; Lawal, I.T.; Ibrahim, A.; Ogunrinola, K.Y. 2017 Ascorbic acid ameliorates behavioural deficits and neuropathological alterations in ratmodel of Alzheimer’s disease. Environ. Toxicol. Pharmacol. 50, 200–211
• Palmer, H.J. and Paulson, E.K. (1997). Reactive oxygen species and Antioxidants in Signal Transduction and Gene expression. Nutr.Rev.; 353 – 361
• Puty, B.; Maximino, C.; Brasil, A.; da Silva, W.L.; Gouveia, A., Jr.; Oliveira, K.R.; Batista Ede, J., Crespo-Lopez, M.E.; Rocha, F.A.; Herculano, A.M. (2014). Ascorbic acid protects against anxiogenic-like effect induced by methylmercury in zebrafish: Action on the serotonergic system. Zebrafish 11, 365–370
• Reali C, Scintu F, Pillai R, Donato R, Michetti F. and Sogos F. (2005). S100b counteracts effects of the neurotoxicant trimethyltin on astrocytes and microglia. Journal of Neuroscience Research, 81: 677-686
• Risdall, J.E. and Menson, D.K.(2011) Traumatic brain injury. Philos. Trans. R. Soc. Lond. B. Biological Science., 366,241-50.
• Saatman, K. E., Duhaime, A. C., Bullock, R., Maas, A. I., Valadka, A. and Manley, G. T. (2008). Classification of traumatic brain injury for targeted therapies. Journal of Neurotrauma, 25(7), 719-738.
• Shah, A.S., Yoon, G.., Hyun, O.K. and Myeong, O.K. Vitamin C neuroprotection against dose-dependent glutamate induced neurodegeneration in the postnatal brain (2015). Neurochemistry Research, 10 (2) 1-12
• Shahidi, S., Komaki, A., Mahmoodi, M., Atrvash, N. and Ghodrati, M. (2008). Ascorbic acid supplementation could affect passive avoidance learning and memory in rat, Brain Research Bulletin, 76(1-2), 109-13
• Sidaros, A., Engberg, A. W., Sidaros, K., Liptrot, M. G., Herning, M., Petersen, P. and Rostrup, E. (2007). Diffusion tensor imaging during recovery from severe traumatic brain injury and relation to clinical outcome: a longitudinal study. Brain, 131(2), 559-572.
• Sil, S.; Ghosh, T.; Gupta, P.; Ghosh, R.; Kabir, S.N.; Roy, A. 2016 Dual Role of Vitamin C on the NeuroinflammationMediated Neurodegeneration and Memory Impairments in Colchicine Induced Rat Model of Alzheimer Disease. J. Mol. Neurosci., 60, 421–435.
• Stanford, S.C. (2007).The open field test: reinventing the wheel Journal of psychopharmacology, vol.21, no.2, Pp.134-136
• Suleiman, N., Bilbis, L. S., Saidu, Y., Nasiru, J. I., Dallatu, M. K., Sahabi, S. M., Ngaski, A. A., Garba, B., Yakubu, A. S. and Bulama, I. 2015 Effects of Some Low molecular mass antioxidants in the Management of Traumatic brain in Albino rats International Journal of Current Research vol.7, no.7, Pp.18492-18499
• Suleiman, N., Bulama, I., Muhammad, NI., Aishat, DI., Balarabe, SA., Ngaski, AA., Buhari, S., Jimoh, AA., Abbas, AY., Saidu, Y. and Bilbis LS, 2018 Neurochemical Effects of Vitamins C, E and DMSO Combinations on Oxidative Stress Biomarkers and Severity of Ischemic Stroke in Wistar Rats, Archives in Neurology & Neuroscience, vol.1,no.2,
• Susana,G., Guzmán-Beltrán, S., Medina-Campos, ON., Pedraza-Chaverri, J. Curcumin pretreatment induces Nrf2 and an antioxidant response and prevents hemin-induced toxicity in primary cultures of cerebellar granule neurons of rats. Oxidative medicine and cellular longevity. 2013, vol.2013, no. 1, Pp
• Tonelli, C., Chio, II. & Tuveson, DA.2018 Transcriptional regulation by Nrf2 Antioxidants & redox signaling, vol.29, no.17, Pp.1727-45.
• Travica, N., Ried, K., Sali, A., Scholey, A., Hudson, I., Pipingas, A. 2017 Vitamin C Status and Cognitive Function Nutrients, vol.9, no.9, Pp.960. Walker, KR., &Tesco, G. (2013). Molecular mechanisms of cognitive dysfunction following traumatic brain injury. Frontiers in aging neuroscience, , vol.5, no.1, P.29.