Effects of vitamin E on calcium signaling and oxidative injury in neutrophils of patients with ischemia/reperfusion (surgical arthroscopy) under sevoflurane anesthesia

Year 2024, Volume: 16 Issue: 2, 1205 - 1213, 17.10.2024

Hacı Ömer Osmanlıoğlu , Lütfi Yavuz , Bilal Çiğ , Mustafa Nazıroğlu

https://doi.org/10.37212/jcnos.1569027

Abstract

Sevoflurane is an anesthetic, and it acts on oxidative activity by activating Ca2+ influx. In human neutrophils, oxidative stress activates the voltage-gated calcium channels (VGCC) and the TRPM2 channel; on the other hand, these channels are inhibited by 2-aminoethoxydiphenyl borate (2-APB) and verapamil plus diltiazem (V+D), respectively. Under sevoflurane anesthesia, surgical arthroscopy poses a significant risk to oxidative stress and Ca2+ influx-induced neutrophil infiltration and injury of patients. However, vitamin E may inhibit lipid peroxidation (LP) by upregulating reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) but downregulating TRPM2 and VGCC in the neutrophils of surgical arthroscopy patients. This topic was examined in the current study. We enrolled 20 patients in the current study, separating them into two primary groups: patients and patients plus vitamin E. Ten patients were divided into two groups: preoperative (N1) and postoperative (N2), both of which were not given vitamin E therapy. The remaining ten patients were given 300 IU of vitamin E two hours prior to their surgical arthroscopy (E1), and their blood was again drawn following the procedure (E2). Prior to fMLP stimulation, the isolated neutrophils from each of the four groups were cultured with 10 uM V+D and 100 uM 2-APB. In the neutrophils, there was an increase in intracellular free Ca2+ ([Ca2+]i) concentration and LP levels due to the downregulation of GSH and GSH-Px; however, following vitamin E treatment, GSH concertation and GSH-Px activity increased in the E2 group. While 2-APB and V+D treatment reduced the concentration of [Ca2+]i in the neutrophils, vitamin E administration had no effect on this measurement. In summary, vitamin E treatment mitigated the GSH and GSH-Px alterations induced by I/R damage, while TRPM2 and VGCC inhibition reduced the [Ca2+]i rise induced by I/R injury. One potential treatment approach for I/R-induced oxidative neutrophil damage is the suppression of TRPM2 and VGCC.

Keywords

Ischemia/reperfusion injury, Neutrophil, Sevoflurane, TRPM2 channel, Vitamin E

Ethical Statement

The Suleyman Demirel University in Isparta, Türkiye's local human ethics committee accepted the project

Supporting Institution

The Scientific Research Unit (BAP) of Suleyman Demirel University, Isparta, Türkiye provided financial assistance for this study (Project No: 2381-TU-10)

Project Number

2381-TU-10

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