Ecophysiological Study on Soil Cyanobacterium in Combination of pH and Salinity Conditions at Limited Irradiance

Year 2020, Volume: 2 Issue: 1, 34 - 45, 15.06.2020

Sarah Jorjani , Ehsan Etesami , Shadman Shokravi

Abstract

The aim of this research was to study ecophysiological cyanobacterium Hapalosiphon sp. FS56 collected from paddy fields of Golestan province in north of Iran. In this attempt, the purified strain was incubated in the BG011 culture medium under illumination (2 μmol quanta.m-2 .S-1) with pH 7.5 and 25 2 C temperature. In order to assess photosynthesis and morphological parameters such as growth rate, pigment and phycobilisome content, some treatments under the combination effects of pH (5,7,9), salinity condition (0, 0.25, 0.5% and 1%) at the limited irradiance (2 μmol quanta.m-2 .S-1) intensities were implemented.
The Results showed that Hapalosiphon sp. Fs56 had the highest growth rate under 0.5% salinity (g/l-1), and light-saturated photosynthetic rate peaked at this salinity concentration as well. This optimal growth rate obtained at pH 9 indicated that this soil cyanobacterium is more susceptible to alkaline conditions. It should be noted that this cyanobacterium showed changes for appearance and morphology under various environmental conditions pH and salinity.

Keywords

Cyanobacteria, Hapalosiphon, Salinity, Light, Acidity and alkalinity

Sınırlı Işınımda pH ve Tuzluluk Koşullarının Kombinasyonu ile Toprak Siyanobakteri Üzerinde Ekofizyolojik Bir Araştırma

Abstract

Bu araştırmanın amacı İran'ın kuzeyinde Golestan eyaletinin çeltik tarlalarından toplanan ekofizyolojik siyanobakteri Hapalosiphon sp. FS56 nın araştırılmasıdır. Bu denemede saflaştırılmış örnekleri, BG011 kültür ortamı pH 7.5 ve 25 ° C sıcaklık koşullarında aydınlatma altında (2 umol kuant.m-2.S-1) inkübe edildi. Fotosentez ve büyüme hızı, pigment ve fikobilizom içeriği gibi morfolojik parametreleri değerlendirmek için sınırlı ışınlamada koşullarında (2 µmol quanta.m-2.S-1); pH (5,7,9) ve farklı tuzluluk durumu (% 0,025,% 0,5 ve% 1) kombinasyonları uygulanarak etkileri incelenmiştir. Sonuçlar, Hapalosiphon sp. Fs56 nın % 0.5 tuzluluk (g / l-1) altında en yüksek büyüme oranına sahip olduğunu ve doygun ışık fotosentez oranında bu tuzluluk konsantrasyonunda en yüksek noktaya ulaştığını gösterdi. PH 9'da elde edilen bu optimal büyüme oranı, bu toprak siyanobakterisinin alkalin koşullarına daha duyarlı olduğunu göstermiştir. Bu siyanobakterinin, çeşitli çevresel pH ve tuzluluk koşulları altında görünümünde ve morfolojisinde değişiklikler gösterdiği fark edilmelidir.

Keywords

Siyanobakteri, hapalasifon, tuzluluk, ışık, asidete ve baziklik, morfoloji, İran

References

• • Aiyer, R.S. (1965). Cmparative algological studies in rice fiels in Kerala State. Agri. Res. J. Kerala 3: 100-105
• • Roger, P.A., Kulasooriya, S.A. (1980). Blue-Green algae and rice. The international rice research inshtue.Los Banos.Laguna, Philipines
• • Shokravi Sh., Safaei M., Jorjani S. 2010. Studying of acclimation of the cyanobacterium Haplosiphon sp. FS 44 to the combination effects of pH and carbon dioxide concentration. Quart. J. Plant Sci. Res. 5(3): 31-42.
• • Vijaya, V. and Narayanaswamy, A. (2009). Blue light enhances the pigment synthesis in cyanobacterium Anabaena ambigua rao (nostacales). ARPN Jornal of Agicultural and Biological Science. VOL. 4, NO. PP36-43
• • Shokravi Shadman and Arian Satei (2007), Investigation of morphological potential of cyanobacteria for inoculation in paddy fields, Research Report, Research Deputy of Islamic Azad University, Gorgan Branch.
• • Veliente, E.F., Leganes, F. (1989). Regulatory effect of pH and incident irradiance on the levels of Nitrogenase activity in the cyanobacterum Nostoc. UAM 205- J. Plant Physiol. 135:623-627
• • Soltani N., Khavinejad, RA, Tabatabaei Yazdi, M., Shokravi, Sh., And Fernandes Valente, A. (2005), Investigation of Antimicrobial Properties and Physiology of Cyanobacteria in Extreme Environments, PhD Thesis, Department of Biology, Faculty of Science, Tarbiat Moallem University, Tehran.
• • Shokrovi, Sh. And emitter, a. (2005) Morphological characterization of cyanobacteria for inoculation in paddy fields, Research Report, Research Deputy of Islamic Azad University, Gorgan Branch.
• • Shokrovi, Sh. Soltani, n. Braided chl. (2008) Cyanobacteriology, First Chab, Islamic Azad University, Gorgan Branch.
• • Jorjani,S.,Shokravi,Sh.,Kouhsari,M.,Ahmadi,H.,Kaviani,A.(2012) Studying of morphological variations of soil cyanobacterium Hapalosiphon sp.FS56 to combination effects of pH and relatively limited carbon dioxide at the extreme limited irradiances.Journal on Plant Science Researches,Serial26,7th year,Number2,Summer 2012.
• • Soltani, N., Khavari-Nejad, R., TabatabaeiYazdi, M., Shokravi, Sh., Fernández-Valiente, E. (2005).
• • Safaei, M., Shokravi, Sh., Ulamai, M. Soltani, N. (2007) Evaluation of survival and growth and pigment status of cyanobacteria Fischerella sp. Under different salinity conditions, M.Sc., Islamic Azad University, Gorgan Branch
• • Amir Latifi, F., Shokravi, Sh., Ulama, M. (2007) Survey of survival and growth and pigment status of cyanobacteria Nostoc sp. In Different Conditions of Acidity and Alkalinity, M.Sc., Islamic Azad University, Gorgan Branch.
• • Etesami, E., Noroozi, M., Jorjani, S., 2020. Research on Surirella brebissonii as a biofuel alternative. Forestist 70(1): 19-27.
• • Kaushik, B.D. (1987). Laboratory methods for blue-green algae. Associated Publishing Company, New Delhi, India.
• • John, D.M., Whitton, B.W. & Brook, A.J. (2003). The Freshwater Algal Flora of the British Isles-Cambridge University Press.
• • Anagnostidis, K. & Komarek, J. (1990). Modern approaches to the classification of cyanobacteria. Stigonematales. Archieves for Hydrobiology sup l4, 224-286.
• • Prescott, G.W. (1962). Algae of the western great lake area. W.M.C. Brown Company Pub.
• • Desikachary, T.V. (1959). Cyanophyta. Indian council of agricultural research, monographs on Algae New Delhi, India.
• • Geitler, L. (1932). Cyanophyceae von Europa Kryptogamen flora Akademiche Verlagsgesellschaft. Leipzig.
• • Soltani, N., Khavari-Nejad, R., Tabatabaie, M., Shokravi, Sh., Valiente, E.F. (2006). Variation of Nitrogenase Activity, photosynthesis and pigmentation of cyanobacterium Fischerellaambigua strain FS18 under different irradiance and pH. World Microbiol.Biotechnol., 22 (6): 571-576.
• • Vincent, W.F., Howard-Williams, C. (2001). Life on snowball Earth –science 287: 2421.
• • Leganés, F. & Fernández-Valiente, E. (1991). The relationship between the availability of external CO2 and nitrogenase activity in the cyanobacterium Nostoc UAM205. Journal of Plant Physiology139, 135-139.
• • Poza-Carrión, C., Fernández-Valiente, E., Piñas, F.F. &Leganés, F. (2001) Acclimation to photosynthetic pigments and photosynthesis of the cyanobacteriumNostoc sp. strain UAM206 to combined fluctuations of irradiance, pH, and inorganic carbon availability.Journal of Plant Physiology158, 1455-14
• • Amir-latifi, F. (2007). 'Study of survival and growth and pigmentation status of Cyanobacterium Nostoc sp. in different situations of acidity and alkalinity.' M.Sc. thesis, Islamic Azad University, Gorgan Branch.
• • Shokravi, Sh., Soltani, N. And weaving, L. (2002) Developing Technology for Using Cyanobacteria as Biological Fertilizer in Paddy Fields, Supreme Research Council of the Presidential Institution (National Plan) Research Institute of Applied Science, Shahid Beheshti University.
• • Sasani, Z., Shokravi, SH, Khalilzadeh, R. Ostei, A. (2009) Microalgae feeding potential as bio-shields in agriculture under extreme carbon dioxide conditions caused by potential attacks, MSc Thesis, University. Islamic Azad Gorgan Branch.
• • Shakeri, Z, Shokravi, Sh, Mola'i Rad, A. Soltani, N. (2009). Evaluation of Extreme Acidic and Acidic Conditions Due to Chemical Bombardment in Protected Microalgae in Agricultural Land Master's Degree, Islamic Azad University.
• • Soltani N., Zarrini G., Ghasemi Y., Shokravi Sh., Baftechi L.( 2009). Characterization of soil cyanobacterium Fischerella sp. FS18 under NaCl stress. J. Biol. Sci. 7(6): 931—936.
• • Shokravi, Sh. (1999). A taxonomic study of cyanobacteria in paddy fields of Golestan province. Eighth Iranian Biological Conference. University of Tehran.
• • Shokravi, Sh., Safai, M. (1388). Ecophysiological study of Hapalosiphon sp. Cyanobacteria collected from paddy fields of Golestan province. Report of Research Project of Research Deputy, Islamic Azad University of Gorgan.
• • Schwarz R., and Forchhamer K. (2005). Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses Microbiology 151: 2503-2514.
• • Nisha R., Kaushik A., Kaushik C.P. (2007). Effect of indigenous cyanobacterial application on structural stability and productivity of and organically poor semi-arid soil. Geoderma 138:49-56.
• • Vakili, F., Shokravi, Sh., Ghourchi Beigi, K. And Sultani, n. (2006) Evaluation of Growth and Heterocyst Status in Fischerella Ambigua Cyanobacterium M.Sc., Department of Biology, Islamic Azad University, Gorgan Branch.
• • Perona E.; Abol, M.; Bonilla,I. and Mateol, P. (2003). Cyanobacterial diversity in Spanish river determined by means of isolation cultures. Morphological variability of isolates in relation to natural populations. Algological Studies 109 Cyanobacterial research 4 pp:475 486.
• • Shokravi, Sh., F. Amirlatifi, M. Safaie, et al. (2006). Some physiological responses of Nostoc sp. JAH 109 to the combined effects of limited irradiance, pH and DIC availability. Q. J. Plant Sci. Res. 1(3): 55-63.