Inhibition of Pseudomonas aeruginosa Biofilm Formation and Quorum Sensing System by Extracts of Prunus avium Stalk

Year 2021, Volume: 33 Issue: 2, 187 - 194, 31.03.2021

Ayla Yıldız , Arhun Ali Balkan , Didem Berber , Barış Gökalsın , Cenk Sesal

https://doi.org/10.7240/jeps.741412

Abstract

Recently, misuse or overuse of antibiotics has led to the antibiotic resistance problem, a global healthcare problem. Most virulence factors and biofilm formation in Pseudomonas aeruginosa are controlled by quorum sensing (QS). The inhibition of QS system by inhibitor molecules has been suggested as a novel alternative antivirulence approach in which no need to kill the bacteria. In the present study, QS and biofilm inhibitory potentials of the methanol and acetone extracts of Prunus avium stalk against P. aeruginosa were evaluated. The extracts were tested at the concentrations of 240, 120, and 60 μg/ml. lasB-gfp, rhlA-gfp, pqsA-gfp biosensor strains and P. aeruginosa PAO1 were used to monitor QS and biofilm inhibition, respectively. Fluorescence and absorbance measurements were performed on Cytation 3 multimode microplate reader. QS inhibition ratios for las, rhl, and pqs systems and biofilm inhibition ratios of the acetone extracts were recorded as 70.43%, 47.25%, 76.31%, and 47.76% (±6,60) and of the methanol extracts as 74.96%, 40.10%, 71.89%, and 38.54% (±3,56) at a certain concentration of 240 μg/ml, respectively. As a result, anti-QS and anti-biofilm properties of acetone extracts were better than that of methanol extracts. Further investigations are needed to discover inhibitor compounds of P. avium and also their effects on human cells and then these compounds may be used in new drug discoveries.

Keywords

Pseudomonas aeruginosa, quorum sensing, biofilm, quorum quenching, Prunus avium stalk, sweet cherry

Prunus avium Sapından Elde Edilen Özütlerle Pseudomonas aeruginosa’ya ait Quorum Sensing Sisteminin ve Biyofilm Oluşumunun İnhibisyonu

Abstract

Öz
Son zamanlarda, antibiyotiklerin yanlış veya aşırı kullanımı, küresel bir sağlık sorunu olan antibiyotik direnci sorununa yol açmıştır. Pseudomonas aeruginosa'da çoğu virülans faktörü ve biyofilm oluşumu, quorum sensing (QS) ile kontrol edilir. QS sisteminin inhibitör moleküller tarafından inhibisyonu, bakterileri öldürmeye gerek olmayan yeni bir alternatif antivirulens yaklaşımı olarak önerilmiştir. Bu çalışmada, Prunus avium sapından elde edilen metanol ve aseton özütlerinin P. aeruginosa'ya karşı QSI (QS inhibitörleri) ve anti-biyofilm potansiyellerini değerlendirdik. Ekstraktlar 240, 120 ve 60 μg/ml'lik konsantrasyonlarda test edilmiştir. QS ve biyofilm inhibisyonunu izlemek için lasB-gfp, rhlA-gfp, ve pqsA biyosensör suşları ve P. aeruginosa PAO1 kullanıldı. Floresans ve absorbans ölçümleri Cytation 3 çok modlu mikroplaka okuyucu üzerinde gerçekleştirildi. 240 μg/ml konsantrasyonunda aseton özütlerinin las, rhl ve pqs sistemleri üzerine QS ve biyofilm inhibisyon oranları sırasıyla % 70.43, % 47.25, % 76.31 ve % 47.76 (± 6,60) ve metanol özütlerinin sırasıyla % 74.96, % 40.10, % 71.89 ve % 38.54 (± 3,56) olarak kaydedilmiştir. Sonuç olarak, aseton özütlerinin anti-QS ve anti-biyofilm özellikleri metanol özütlerinden daha başarılı olmuştur. P. avium’un inhibitör bileşikleri ve bu bileşiklerin insan hücreleri üzerindeki etkilerini keşfetmek için daha fazla araştırmaya ihtiyaç vardır ve daha sonra bu bileşikler yeni ilaç keşiflerinde kullanılabilir.

Keywords

Pseudomonas aeruginosa, quorum sensing, biofilm, quorum quenching, Prunus avium stalk, sweet cherry

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