BIOACTIVE COMPONENTS OF AVOCADO AND ITS HEALTH EFFECTS

Year 2022, Volume: 10 Issue: 1, 341 - 359, 23.03.2022

Lale Gümüştepe , Ebru Aydın , Gülcan Özkan

https://doi.org/10.21923/jesd.1005610

Cited By: 4

Abstract

Avocados belong to the Plantae kingdom, the Lauraceae family, the order Laurales, the genus Persea, and the species P. americana. It is a plant-derived food which is implicated in various health-promoting and disease preventive effects. Therefore, it is considered a superfood. Avocado fruit pulp and peel, leaves and seeds are rich in both nutritive, such as vitamins, dietary fibers, and mineral and bioactive compounds, polyphenols, carotenoids, tocopherols and sterols. While carotenoids and tocopherols are mostly found in the fruit pulp, polyphenols are widely present in the fruit pulp and peel, seeds and leaves. Avocado fruit's seed (16%) and peel (11%) are consisted of 21-30% of the fruit as a solid waste except in some varieties.
The rich bioactive compounds of avocado exhibited important biological activities in in vitro and in vivo studies, suggesting possible prevention against several diseases such as hypoglycemic, antihypertensive, antioxidant, obesity, hyperlipidemic, antimicrobial, antiprotozoal and antimycobacterial activity etc. Therefore, its effect on diseases has become a subject of interest in the literature. It was reported that the seed, peel and leaf of the fruit, especially from the avocado waste, have larvicidal, antifungal, antimicrobial, antioxidant, antiprotozoal, antidiabetic, antihypertensive, hypercholesterolemic and antimycobacterial activities such as stopping/slowing down many diseases. In this review, avocado tree; the bioactive components of the fruit pulp and peel, leaves and seeds and the effects of these components on health will be examined.

Keywords

Avocado, Bioactive Components, Metabolic Syndrome, By-Products.

AVOKADONUN BİYOAKTİF BİLEŞENLERİ VE SAĞLIK ÜZERİNE ETKİLERİ

Abstract

Avokado, Plantae krallığına, Lauraceae ailesine, Laurales takımına, Persea cinsine ve P. americana türüne ait bir bitkidir. İçeriğindeki zengin biyoaktif bileşenlerin ve besin öğelerinin sağlık üzerine yararlı etkileri olduğu bilinen ve süper gıda olarak kabul edilen bitkisel kaynaklı bir gıdadır. Avokado ağacının; meyvesinin eti ve kabuğu, yaprağı ve çekirdeği zengin biyoaktif bileşenler içermektedir. Meyve çekirdeği (%16) ve kabuğu (%11) bazı çeşitlerde istisnalar olmak üzere meyvenin %21-30’unu temsil eden miktarlarda katı atık oluşturmaktadır. Avokadoda en yaygın bulunan biyoaktif bileşenler polifenoller, karotenoitler, tokoferoller ve sterollerdir. Polifenoller meyve eti, kabuk, tohum ve yapraklarda; karotenoitler ve tokoferoller ise avokado meyvesinin etinde bulunmaktadır. Avokadonun zengin biyoaktif içeriğinin metabolik sendrom hastalıklarına, hipoglisemik, antihipertansif, antioksidan, obezite, hiperlipidemik, antimikrobiyal, antiprotozoal ve antimikobakteriyel vb. hastalıklara etkisi literatürde ilgi çeken bir konu haline gelmiştir. Özellikle avokado atıklarından çekirdeğinin, meyvesinin kabuğunun ve yaprağının larvisidal, antifungal, antimikrobiyal, antioksidan, antiprotozoal, antidiyabetik, antihipertansif, hiperkolesterolemik ve antimikobakteriyel aktiviteler gibi birçok hastalığı durdurucu/yavaşlatıcı etkisinin olduğu bildirilmiştir. Bu derlemede, avokado ağacının; meyvesinin eti ve kabuğunun, yaprağının ve çekirdeğinin biyoaktif bileşenleri ve bu bileşenlerin sağlık üzerine etkileri incelenecektir.

Keywords

Avokado, Biyoaktif Bileşenler, Metabolik Sendrom, Yan Ürünler.

References

• Abe, R., Ohtani, K., 2013. An Ethnobotanical Study of Medicinal Plants and Traditional Therapies on Batan Island, the Philippines. Journal of Ethnopharmacology 145 (2): 554–65.
• Adeboye, J. O., Fajonyomi, M.O., Makinde, J. M. ve Taiwo, O. B., 1999. A Preliminary study on the hypotensive activity of Persea americana leaf extracts in anaesthetized normotensive rats. Fitoterapia 70 (1): 15–20.
• Adeyemi, O. O., Okpo, S. O. ve Ogunti, O. O., 2002. Analgesic and anti-inflammatory effects of the aqueous extract of leaves of Persea americana Mill (Lauraceae). Fitoterapia 73 (5): 375–80.
• Sargin, A. S., 2015. Ethnobotanical survey of medicinal plants in Bozyazı district of Mersin, Turkey. Journal of Ethnopharmacology 173: 105–126.
• Alarcon-Aguilara, F. J., Roman-Ramos, R., Perez-Gutierrez, S., Aguilar-Contreras, A., Contreras-Weber, C. C. ve Flores-Saenz, J. L., 1998. Study of the anti-hyperglycemic effect of plants used as antidiabetics. Journal of Ethnopharmacology 61 (2): 101–10.
• Alhassan, A. J., Sule, M. S., Atiku, M. K., Wudil, A. M., Abubakar, H. ve Mohammed, S. A., 2012. Effects of aqueous avocado pear (Persea americana) seed extract on alloxan induced diabetes rats. Greener Journal of Medical Sciences 2 (1): 005–011.
• Alkhalaf, M. I., Alansari, W. S., Ibrahim, E. A. ve ELhalwagy, M.E.A., 2019. Anti-oxidant, anti-inflammatory and anti-cancer activities of avocado (Persea americana) fruit and seed extract. Journal of King Saud University - Science 31 (4): 1358–62.
• Almeida, A. P. D., Miranda, M.M.F.S., Simoni, I. C., Wigg, M. D., Lagrota, M. H.C. ve Costa, S. S., 1998. Flavonol Monoglycosides Isolated from the Antiviral Fractions of Persea americana (Lauraceae) Leaf Infusion. Phytotherapy Research 12 (8): 562–67.
• Anbar, D., 2014. Li̇paz Enzi̇mi̇ne Çeşi̇tli̇ Bi̇tki̇ Ekstreleri̇ni̇n Etki̇si̇. (Yüksek Lisans Tezi), Marmara Üniversitesi.
• Ant, A., Avcı, A., Genç, M., İnal, E., Tunçel, Ü. ve Şencan, Z., 2017. Avokado yaprağı ekstresinin larinks kanseri dokularında Adenozin Deaminaz (ADA) üzerine aktive edici etkisi.” Acta Oncologica Turcica 51 (2): 199–204.
• Antasionasti, I., Riyanto, S. ve Rohman, A., 2017. Antioxidant Activities and Phenolics Contents of Avocado (Persea americana Mill.) Peel in vitro. Research Journal of Medicinal Plants 11 (2): 55–61.
• Araújo, R. G., Rodriguez-Jasso, R. M., Ruiz, H. A., Govea-Salas, M., Pintado, M. E. ve Aguilar, C. N., 2020. Process optimization of microwave-assisted extraction of bioactive molecules from avocado seeds. Industrial Crops and Products 154 (May): 112623.
• Araújo, R. G., Rodriguez-Jasso, R. M., Ruiz, H. A., Pintado, M. M. E., and Aguilar, C. N., 2018. Avocado by-products: Nutritional and functional properties. Trends in Food Science and Technology 80: 51-60. Elsevier Ltd.
• Arukwe, U., Amadi, B.A., Duru, M.K.C., Agomou, E.N., Adindu, E. A., Odika, P.C., Lele, K.C., Egejuru, L. ve Anudike, J., 2012. Chemical Composition of Persea americana Leaf, Fruit and Seed. Ijrras 11 (2): 346–49.
• Barbosa-Martín, E., Chel-Guerrero, L., González-Mondragón, E. ve Betancur-Ancona, D., 2016. Chemical and technological properties of avocado (Persea americana Mill.) seed fibrous residues. Food and Bioproducts Processing 100: 457–63.
• Barut Uyar, B. ve Sürücüoğlu, M. S., 2010. Besi̇nlerdeki̇ Bi̇yoloji̇k Aktif Bi̇leşenler. J Nutr and Diet 38 (1–2): 69–76.
• Bayram, S., 2010. Avokado (Persea Americana Mill.). BATEM. 2010 Yılı Avokado Gelişim Raporu.
• Bayram, S., Tepe, S. ve Toker, R., 2016. Hasat periyodu boyunca Hass avokado çeşidinin meyvelerinde bazı fiziksel ve kimyasal değişimlerin belirlenmesi. Derim 33 (1): 14-26.
• Bouic, P. J. D., 2002. Sterols and Sterolins: new drugs for the immune system?. Drug Discovery Today 7 (14): 775–778.
• Boulton, A. J.M., Malik, R. A., Arezzo, J. C. ve Sosenko, J. M., 2004. Diabetic Somatic Neuropathies. Diabetes Care 27 (6): 1458–86.
• Brai, B. I.C., Adisa, R. A. ve Odetola, A. A., 2014. Hepatoprotective Properties of Aqueous Leaf Extract of Persea americana Mill. (Lauraceae) ‘Avocado’ Against CCL4-Induced Damage In Rats. African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM / African Networks on Ethnomedicines 11 (2): 237–44.
• Brai, B. I.C., Odetola A.A. ve Agomo, P.U., 2007. Hypoglycemic and Hypocholesterolemic Potential of Persea americana Leaf Extracts. Journal of Medicinal Food 10 (2): 356–60.
• Brovelli, E. A., Vallejos, J. A., Ikonte, C., Menon, G. R., Fast, D. J. ve Chandra, A., 2005. Compositions Containing Avocado Leaf Extract For Lowering Cholesterol Levels. 1 (19).
• Brufau, G., Canela, M. A. ve Rafecas, M., 2008. Phytosterols: Physiologic and Metabolic Aspects Related to Cholesterol-Lowering Properties. Nutrition Research 28 (4): 217–25.
• Butt, A. J., Roberts, C. G., Seawright, A. A., Oelrichs, P. B., MacLeod, J. K., Liaw, T.Y.E., Kavallaris, M., Somers-Edgar, T.J., Lehrbach, G.M., Watts, C.K. VE Sutherland, R.L., 2006. A Novel Plant Toxin, Persin, with in vivo Activity in the Mammary Gland, Induces Bim-Dependent Apoptosis in Human Breast Cancer Cells. Molecular Cancer Therapeutics 5 (9): 2300–2309.
• Carpentier, S., Knaus, M. ve Suh, M., 2009. Associations between Lutein, Zeaxanthin, and Age-Related Macular Degeneration: An Overview. Critical Reviews in Food Science and Nutrition 49 (4): 313–26.
• Castro-López, C., Bautista-Hernández, I., González-Hernández, M. D., Martínez-Ávila, G. C.G., Rojas, R., Gutiérrez-Díez, A., Medina-Herrera, N. ve Aguirre-Arzola, V. E., 2019. Polyphenolic Profile and Antioxidant Activity of Leaf Purified Hydroalcoholic Extracts from Seven Mexican Persea Americana Cultivars. Molecules 24 (1).
• Cerda-Opazo, P., Gotteland, M., Oyarzun-Ampuero, F. A. ve Garcia, L., 2021. Design, development and evaluation of nanoemulsion containing avocado peel extract with anticancer potential: A novel biological active ingredient to enrich food. Food Hydrocolloids 111 (September 2020): 106370.
• Chávez, F., Aranda, M., García, A. ve Pastene, E., 2011. Antioxidant polyphenols extracted from Avocado epicarp (Persea americana var. Hass) inhibit Helicobacter Pylori urease. Boletin Latinoamericano y Del Caribe de Plantas Medicinales y Aromaticas 10 (3): 265–80.
• Cires, M. J., Navarrete, P., Pastene, E., Carrasco-Pozo, C., Valenzuela, R., Medina, D. A., Andriamihaja, M., Beaumont, M., Blachier, F. ve Gotteland, M., 2019. Protective Effect of an Avocado Peel Polyphenolic Extract Rich in Proanthocyanidins on the Alterations of Colonic Homeostasis Induced by a High-Protein Diet. Journal of Agricultural and Food Chemistry 67 (42): 11616–26.
• Colombo, R. ve Papetti, A., 2019. Avocado (Persea americana Mill.) by‐products and their impact: from bioactive compounds to biomass energy and sorbent material for removing contaminants. A review. International Journal of Food Science & Technology, 54(4), 943–951.
• D’Ambrosio, S. M., Han, C., Pan, L., Kinghorn, A. D. ve Ding, H., 2011. Aliphatic acetogenin constituents of avocado fruits inhibit human oral cancer cell proliferation by targeting the EGFR/RAS/RAF/MEK/ERK1/2 pathway. Biochemical and Biophysical Research Communications 409 (3): 465–69.
• Dabas, D., Shegog, R.M., Ziegler, G.R. ve Lambert, J.D., 2013. Avocado (Persea americana) Seed as a Source of Bioactive Phytochemicals. Current Pharmaceutical Design 19 (34): 6133–40.
• Dabas, D., Ziegler, G.R. and Lambert, J.D., 2019. Anti-Inflammatory Properties of a Colored Avocado Seed Extract. Advances in Food Technology and Nutritional Sciences - Open Journal 5 (1): 8–12.
• Demirkol, A., 1995. Avocado Growing in Turkey. Proceedings of The World Avocado Congress III, 451–56.
• Dike, I.P., Obembe, O.O. ve Adebiyi, F.E., 2012. Ethnobotanical survey for potential anti-malarial plants in south-western Nigeria. Journal of Ethnopharmacology 144 (3): 618–26.
• Ding, H., Chin, Y.W., Kinghorn, A.D. ve D’Ambrosio, S.M, 2007. Chemopreventive characteristics of avocado fruit. Seminars in Cancer Biology 17 (5): 386–94.
• Ding, H., Han, C., Guo, D., Chin, Y.W., Ding, Y., Kinghorn, A. D. ve D’Ambrosio, S.M., 2009. Selective Induction of Apoptosis of Human Oral Cancer Cell Lines by Avocado Extracts via a ROS-Mediated Mechanism. Nutrition and Cancer 61 (3): 348–56.
• Domergue, F., Helms, G. L., Prusky, D. ve Browse, J., 2000. Antifungal compounds from idioblast cells isolated from avocado fruits. Phytochemistry 54: 183–89.
• Domínguez, M. P., Araus, K., Bonert, P., Sánchez, F., San Miguel, G. ve Toledo, M., 2014. The Avocado and Its Waste: An Approach of Fuel Potential/Application. Environment, Energy and Climate Change II, 199–223.
• Dreher, M. L. ve Adrienne J. D., 2013. Hass Avocado Composition and Potential Health Effects. Critical Reviews in Food Science and Nutrition 53 (7): 738–50. Duarte, P. F., Chaves, M. A., Borges, C.D. ve Mendonça, C. R. B., 2016. Avocado: characteristics, health benefits, and uses. International News on Fats, Oils and Related Materials 28 (3): 28–32.
• Edem, D., Ekanem, I. ve Ebong, P., 2009. Effect of aqueous extracts of alligator pear seed (Persea americana Mill.) on blood glucose and histopathology of pancreas in alloxan-induced diabetic rats. Pakistan Journal of Pharmaceutical Sciences 22 (3): 272–76.
• Ejiofor, N. C., Ezeagu, I. E., Ayoola, M. ve Umera, E. A., 2018. Determination of the chemical composition of avocado (Persea americana) seed. Advances in Food Technology and Nutritional Sciences - Open Journal SE (2): S51–55.
• Eroğlu Özkan, E. ve Tavlı, Ö. F., 2020. Ülkemiz Kültür Bitkilerinden Persea americana Mill. (Avokado) ve Tıbbi Açıdan Değerlendirilmesi. Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi ve Folklorik Tıp Dergisi 10 (1): 28–36.
• Ezejiofor, A. N., Okorie, A. ve Orisakwe, O. E., 2013. Hypoglycaemic and Tissue-Protective Effects of the Aqueous Extract of Persea americana Seeds on Alloxan-Induced Albino Rats. Malaysian Journal of Medical Sciences 20 (5): 31–39.
• Falodun, A., Imieje, V., Erharuyi, O., Ahomafor, J., Melissa R. Jacob, M. R., Khan, S. I. ve Hamann, M. T., 2014. Evaluation of Three Medicinal Plant Extracts Against Plasmodium Falciparum and Selected Microganisms. African Journal of Traditional, Complementary and Alternative Medicines 11 (4): 142–46.
• FAO, 2018. http://www.fao.org/3/ca2320en/CA2320EN.pdf
• Figueroa, J. G., Borrás-Linares, I., Lozano-Sánchez, J. ve Segura-Carretero, A., 2018. Comprehensive identification of bioactive compounds of avocado peel by liquid chromatography coupled to ultra-high-definition accurate-mass Q-TOF. Food Chemistry 245 (November 2017): 707–16.
• Fitzmaurice, C., Dicker, D., Pain, A., Hamavid, H., Moradi-Lakeh, M., MacIntyre, M. F., Allen, C., Hansen, G., Woodbrook, R., … ve Wolfe, C., 2015. The Global Burden of Cancer 2013. JAMA Oncology 1 (4): 505–27.
• Fulgoni, V. L., Dreher, M. ve Davenport, A. J., 2013. Avocado Consumption Is Associated with Better Diet Quality and Nutrient Intake, and Lower Metabolic Syndrome Risk in US Adults: Results from the National Health and Nutrition Examination Survey (NHANES) 2001-2008. Nutrition Journal 12 (1): 1–6.
• García-Aguirre, K. K., Zepeda-Vallejo, L. G., Ramón-Gallegos, E., Alvárez-González, I. ve Madrigal-Bujaidar, E., 2008. Genotoxic and Cytotoxic Effects Produced by Acetogenins Obtained from Annona cherimolia Mill. Biological and Pharmaceutical Bulletin 31 (12): 2346–49.
• Gómez, F. S., Sánchez, S.P., Iradi, M.G.G., Azman, N.A.M. ve Almajano, M. P., 2014. Avocado seeds: extraction optimization and possible use as antioxidant in food. Antioxidants 3 (2): 439–54.
• Gross, J., Gabai, M., Lifshitz, A. ve Sklarz. B., 1973. Carotenoids in Pulp, Peel and Leaves of Persea americana. Phytochemistry 12 (9): 2259–63. Gutierrez, M.L. ve Villanueva, M., 2007. The avocado in the prehispanic time avocado in the prehispanic time. Proceeding VI World Avocado Congress, no. 978: 12–16.
• Guzmán-Rodríguez, J. J., López-Gómez, R., Suárez-Rodríguez, L. M., Salgado-Garciglia, R., Rodríguez-Zapata, L. C., Ochoa-Zarzosa, A. ve López-Meza, J. E., 2013. Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus. BioMed Research International 2013.
• Holst, B. ve Williamson, G., 2008. Nutrients and phytochemicals: from bioavailability to bioefficacy beyond antioxidants. Current Opinion in Biotechnology 19 (2): 73–82.
• Hozawa, A., Jacobs, D. R., Steffes, M. W., Gross, M. D., Steffen, L. M. ve Lee, D. H., 2007. Relationships of circulating carotenoid concentrations with several markers of inflammation, oxidative stress, and endothelial dysfunction: The Coronary Artery Risk Development in Young Adults (CARDIA)/Young Adult longitudinal Trends in Antioxidants (YALT) study. Clinical Chemistry 53 (3): 447–55.
• Ikpeme, E .V., Ekaluo, U.B., Udensi, O.U., Ekerette, E.E., 2014. Screening fresh and dried fruits of avocado pear (Persea americana) for antioxidant activities: An alternative for synthetic antioxidant. Journal of Life Sciences Research and Discovery 1 (April): 19–25.
• Imafidon, K. E. ve Amaechina, F.C., 2010. Effects of Aqueous Seed Extract of Persea americana Mill. (Avocado) on Blood Pressure and Lipid Profile in Hypertensive Rats. Advances in Biological Research 4 (2): 116–21.
• Irawati, N. A. V., 2015. Antihypertensive Effects of Avocado Leaf Extract (Persea americana Mill.). Majority 4: 44–48.
• Isaac, A. T., Ganiyu, O., Akinyemi, A. J., Ajani, R. A. ve Olanrewaju, B. O., 2014. Avocado pear fruits and leaves aqueous extracts inhibit α-amylase, α-glucosidase and snp induced lipid peroxidation–an insight into mechanisms involve in management of type 2 diabetes. International Journal of Applied and Natural Sciences (IJANS) 3 (5): 21–34.
• Jiménez-Arellanes, A., Luna-Herrera, J., Ruiz-Nicolás, R., Cornejo-Garrido, J., Tapia, A. ve Yépez-Mulia, L., 2013. Antiprotozoal and Antimycobacterial Activities of Persea americana Seeds. BMC Complementary and Alternative Medicine 13.
• Jiménez, P., García, P., Bustamante, A., Barriga, A. ve Robert, P., 2017. Thermal stability of oils added with avocado (Persea americana cv. Hass) or olive (Olea europaea cv. Arbequina) leaf extracts during the french potatoes frying. Food Chemistry 221: 123–29.
• Jimenez, P., Garcia, P., Quitral, V., Vasquez, K., Parra-Ruiz, C., Reyes-Farias, M., Garcia-Diaz, D. F., Robert, P., Encina, C. ve Soto-Covasich, J., 2020. Pulp, Leaf, Peel and Seed of Avocado Fruit: A Review of Bioactive Compounds and Healthy Benefits. Food Reviews International 00 (00): 1–37.
• Johnson, E. J., Maras, J. E., Rasmussen, H. M. ve Tucker, K. L., 2010. Intake of Lutein and Zeaxanthin Differ with Age, Sex, and Ethnicity. Journal of the American Dietetic Association 110 (9): 1357–62.
• Alonso-Castro, A.J., Maldonado-Miranda, J. J., Zarate-Martinez, A., Jacobo-Salcedo, M. D. R., Fernández-Galicia, C., Figueroa-Zuñiga, L. A., Rios-Reyes, N. A., Leo´n-Rubio, M. A., Medellı´n-Castillo, N. A., … ve Reyes-Munguia, A., 2012. Medicinal Plants Used in the Huasteca Potosina, México. Journal of Ethnopharmacology 143 (1): 292–98.
• Kawagishi, H., Fukumoto, Y., Hatakeyama, M., He, P., Arimoto, H., Matsuzawa, T., Arimoto, Y., Suganuma, H., Inakuma, T. … ve Sugiyama, K., 2001. Liver Injury Suppressing Compounds from Avocado (Persea americana). Journal of Agricultural and Food Chemistry 49 (5): 2215–21.
• Kendir, G. ve Köroğlu, A., 2018. Evaluation of avocado (Persea americana Mill.) leaves in terms of public health. Marmara Pharmaceutical Journal 22 (3): 347-356.
• Khalifa, N. S., Barakat, H. S., Elhallouty, S. ve Salem, D., 2013. Effect of the Water Extracts of Avocado Fruit and Cherimoya Leaf on Four Human Cancer Cell Lines and Vicia Faba Root Tip Cells. Journal of Agricultural Science 5 (7): 245–54.
• Koller, O.C., 1992. Abacaticultura. Porto Alegre: UFRGS. 138p.
• Kopec, R. E., Cooperstone, J. L., Schweiggert, R. M., Young, G. S., Harrison, E. H., Francis, D. M., Clinton, S. K. ve Schwartz, S. J., 2014. Avocado Consumption Enhances Human Postprandial Provitamin A Absorption and Conversion from a Novel High-β-Carotene Tomato Sauce and from Carrots. Journal of Nutrition 144 (8): 1158–66.
• Krinsky, N. I. ve Johnson, E. J., 2005. Carotenoid actions and their relation to health and disease. Molecular Aspects of Medicine 26 (6): 459–516.
• Kulkarni, P., Paul, R. ve Ganesh, N., 2010. In Vitro Evaluation of Genotoxicity of Avocado (Persea americana) Fruit and Leaf Extracts in Human Peripheral Lymphocytes. Journal of Environmental Science and Health - Part C Environmental Carcinogenesis and Ecotoxicology Reviews 28 (3): 172–87.
• Kumar, A., Kumarchandra, R., Rai, R. ve Sanjeev, G., 2017. Anticlastogenic, radiation antagonistic, and anti-inflammatory activities of Persea americana in Albino Wistar rat model. Research in Pharmaceutical Sciences 12 (6): 488–99.
• Kumar, B. ve Cumbal, L., 2016. UV-Vis, FTIR and antioxidant study of Persea americana (Avocado) leaf and fruit : A comparison. Revista de La Facultad de Ciencias Químicas, 14, 13–20.
• Larijani, L. V., Ghasemi, M., AbedianKenari, S. ve Naghshvar, F., 2014. Evaluating the Effect of Four Extracts of Avocado Fruit on Esophageal Squamous Carcinoma and Colon Adenocarcinoma Cell Lines in Comparison with Peripheral Blood Mononuclear Cells. Acta Medica Iranica 52 (3): 201–5.
• Lee, S. H., Heo, J. S., Lee, M. Y. ve Han, H. J., 2008. Effect of Dihydrotestosterone on Hydrogen Peroxide-Induced Apoptosis of Mouse Embryonic Stem Cells. Journal of Cellular Physiology 216 (1): 269–75.
• Leite, J. J. G., Brito, É. H. S., Cordeiro, R. A., Brilhante, R. S. N., Sidrim, J. J. C., Bertini, L. M., Morais, S. M. D. ve Rocha, M. F. G., 2009. Chemical composition, toxicity and larvicidal and antifungal activities of Persea americana (avocado) seed extracts. Revista Da Sociedade Brasileira de Medicina Tropical 42 (2): 110–13.
• Lima, C. R., Vasconcelos, C. F.B., Costa-Silva, J. H., Maranhão, C. A., Costa, J., Batista, T. M., Carneiro, E. M., Soares, L. A.L., Ferreira, F. ve Wanderley, A. G., 2012. Anti-diabetic activity of extract from Persea americana Mill. leaf via the activation of protein kinase B (PKB/Akt) in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology 141 (1): 517–25.
• Litz, R.E., Raharjo, S.H.T. ve Lim, M. G., 2007. Avocado. Transgenic Crops V, 167-187.
• López-Cobo, A., Gómez-Caravaca, A. M., Pasini, F., Caboni, M. F., Segura-Carretero, A. ve Fernández-Gutiérrez, A., 2016. HPLC-DAD-ESI-QTOF-MS and HPLC-FLD-MS as valuable tools for the determination of phenolic and other polar compounds in the edible part and by-products of avocado.” LWT - Food Science and Technology 73: 505–13.
• Lottenberg, A.M.P., Nunes, V. S., Nakandakare, E. R., Neves, M., Bernik, M., Santos, J. E., Quintão, E.C. R. ve Paulo, S., 2002. Plant sterol ester Efficiency on the Plasma Lipid Reduction in Moderate Hipercholesterolemic Subjects. Arq Bras Cardiol 79 (2): 139–42.
• Lu, Q. Y., Arteaga, J. R., Zhang, Q., Huerta, S., Go, V. L. W. ve Heber, D., 2005. Inhibition of prostate cancer cell growth by an avocado extract: role of lipid-soluble bioactive substances. Journal of Nutritional Biochemistry 16 (1): 23–30.
• Lu, Q. Y., Zhang, Y., Wang, Y., Wang, D., Lee, R. P., Gao, K., Byrns, R. ve Heber, D., 2009. California Hass Avocado: Profiling of Carotenoids, Tocopherol, Fatty Acid, and Fat Content during Maturation and from Different Growing Areas. Journal of Agricultural and Food Chemistry 57 (21): 10408–13.
• Lu, Y. C., Chang, H. S., Peng, C. F., Lin, C. H. ve Chen, I. S., 2012. Secondary metabolites from the unripe pulp of Persea Americana and their antimycobacterial activities. Food Chemistry 135 (4): 2904–9.
• Makopa, M., Mangiza, B., Banda, B., Mozirandi, W., Mombeshora, M. ve Mukanganyama, S., 2020. Antibacterial, Antifungal, and Antidiabetic Effects of Leaf Extracts from Persea americana Mill. (Lauraceae). Biochemistry Research International 2020.
• Pacheco, M. M. M., Gomez, R. L., Garciglia, R. S., Calderon, M. R. ve Muñoz, R. E. M., 2011. Folates and Persea americana Mill. (Avocado). Emirates Journal of Food and Agriculture 23 (3): 204–13.
• Melgar, B., Dias, M. I., Ciric, A., Sokovic, M., Garcia-Castello, E. M., Rodriguez-Lopez, A. D., Barros, L. ve Ferreira, I. C.R.F., 2018. Bioactive characterization of Persea americana Mill. by-Products: A rich source of inherent antioxidants. Industrial Crops and Products 111 (June 2017): 212–18.
• Molina-Garza, Z. J., Bazaldúa-Rodríguez, A. F., Quintanilla-Licea, R. ve Galaviz-Silva, L., 2014. Anti-Trypanosoma cruzi activity of 10 medicinal plants used in northeast Mexico. Acta Tropica 136 (1): 14–18.
• Monge-Rojas, R. ve Campos, H., 2011. Tocopherol and carotenoid content of foods commonly consumed in Costa Rica. Journal of Food Composition and Analysis 24 (2): 202–16.
• Monika, P. ve Geetha, A., 2015. The modulating effect of Persea americana fruit extract on the level of expression of fatty acid synthase complex, lipoprotein lipase, fibroblast growth factor-21 and leptin - A biochemical study in rats subjected to experimental hyperlipidemia and obesity. Phytomedicine 22 (10): 939–45.
• Murathan, Z. T. ve Kaya, A., 2020. Alanya Ekolojik Koşullarında Yetişen İki Farklı Avokado Çeşidinin Bazı Fitokimyasal İçerikleri Ile Antioksidan Aktivitelerinin İncelenmesi. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi 3 (6): 1435–40.
• Niogret, J., Epsky, N. D., Schnell, E. Q., Schnell, R. J., Heath, R. R., Meerow, A. W. ve Kendra, P. E., 2013. Analysis of Sesquiterpene Distributions in the Leaves, Branches, and Trunks of Avocado (Persea americana Mill.). American Journal of Plant Sciences 04 (04): 922–31.
• Oboh, G., Isaac, A. T., Akinyemi, A. J. ve Ajani, R. A., 2014. Inhibition of Key Enzymes Linked to Type 2 Diabetes and Sodium Nitroprusside Induced Lipid Peroxidation in Rats’ Pancreas by Phenolic Extracts of Avocado Pear Leaves and Fruit. International Journal of Biomedical Science 10 (3): 210–18.
• Oboh, G., Odubanjo, V. O., Bello, F., Ademosun, A. O., Oyeleye, S. I., Nwanna, E. E. ve Ademiluyi, A. O., 2016. Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro. Journal of Basic and Clinical Physiology and Pharmacology 27 (2): 131–40.
• Odo, C. E., Nwodo, O. F. C., Joshua, P. E., Ugwu, O. P.C. ve Okonkwo, C. C., 2013. Acute toxicity investigation and anti-diarrhoeal effect of the chloroform–methanol extract of the seeds of Persea americana in albino rats. Journal of Pharmacy Research 6 (3): 331–35.
• Odo, C. E., Nwodo, O. F. C., Joshua, P. E., Ugwu, O. P.C. ve Okonkwo, C. C., 2013. Acute toxicity investigation and anti-diarrhoeal effect of the chloroform–methanol extract of the leaves of Persea americana. Iranian Journal of Pharmaceutical Research 13 (2): 651–58.
• Ojewole, J., Kamadyaapa, D. R., Gondwe, M. M., Moodley, K. ve Musabayane, C. T., 2007. Cardiovascular effects of Persea americana Mill (Lauraceae) (avocado) aqueous leaf extract in experimental animals. Cardiovascular Journal of South Africa 18 (2): 69–76.
• Oluwole, F. S., Onasanwo, S. A. ve Olaleye, S. B., 2011. Effects of Aqueous and Methanolic Extracts of Persea americana Leaf (Avocado Pear) On Gastric Acid Secretion In Male Albino Rats. European Journal of Scientific Research 61 (4): 474–81.
• Ortega-Arellano, H. F., Jimenez-Del-Rio, M. ve Velez-Pardo, C., 2019. Neuroprotective Effects of Methanolic Extract of Avocado Persea americana (var. Colinred) Peel on Paraquat-Induced Locomotor Impairment, Lipid Peroxidation and Shortage of Life Span in Transgenic knockdown Parkin Drosophila melanogaster. Neurochemical Research 44 (8): 1986–98.
• Ortiz, M.A., Dorantes, A. L., Gallndez, M. J. ve Cárdenas, S. E., 2004. Effect of a Novel Oil Extraction Method on Avocado (Persea americana Mill) Pulp Microstructure. Plant Foods for Human Nutrition 59 (1): 11–14.
• Ovesnâ, Z., Vachálková, A. ve Horváthov, K., 2004. Taraxasterol and β-sitosterol: New naturally compounds with chemoprotective/ chemopreventive effects. NEOPLASMA 55 (6):407-413.
• Owolabi, M. A., Coker, H. A. B. ve Jaja, S. I., 2010. Bioactivity of the phytoconstituents of the leaves of Persea americana. Journal of Medicinal Plants Research 4 (12): 1130–35.
• Owolabi, M. A., Jaja, S. I. ve Coker, H. A. B., 2005. Vasorelaxant action of aqueous extract of the leaves of Persea americana on isolated thoracic rat aorta. Fitoterapia 76 (6): 567–73.
• Pahua-Ramos, M. E., Ortiz-Moreno, A., Chamorro-Cevallos, G., Hernández-Navarro, M. D., Garduño-Siciliano, L., Necoechea-Mondragón, H. ve Hernández- Ortega, M., 2012. Hypolipidemic Effect of Avocado (Persea americana Mill.) Seed in a Hypercholesterolemic Mouse Model. Plant Foods for Human Nutrition 67 (1): 10–16.
• Plaza, L., Sánchez-Moreno, C., Pascual-Teresa, S. D., Ancos, B. D. ve Cano, M. P., 2009. Fatty Acids, Sterols, and Antioxidant Activity in Minimally Processed Avocados during Refrigerated Storage. Journal of Agricultural and Food Chemistry 57 (8): 3204–9.
• Polat Kose, L., Bingol, Z., Kaya, R., Goren, A. C., Akincioglu, H., Durmaz, L., Koksal, E., Alwasel, S. H. ve Gülçin, İ., 2020. Anticholinergic and antioxidant activities of avocado (Folium perseae) leaves–phytochemical content by LC-MS/MS analysis. International Journal of Food Properties 23 (1): 878–93.
• Poovarodom, S., Haruenkit, R., Vearasilp, S., Namiesnik, J., Cvikrová, M., Martincová, O., Ezra, A., Suhaj, M., Ruamsuke, P. ve Gorinstein, S., 2010. Comparative characterisation of durian, mango and avocado. International Journal of Food Science and Technology 45 (5): 921–29.
• Rajeshkumar, S. ve Rinitha, G,. 2018. Nanostructural sharacterization of antimicrobial and antioxidant copper nanoparticles synthesized using novel Persea americana seeds. OpenNano 3 (January): 18–27.
• Rao, U. S. M., 2017. Salutary Potential of Ethanolic Extract of Avocado Fruit on Anomalous Carbohydrate Metabolic Key Enzymes in Hepatic and Renal Tissues of Hyperglycaemic Albino Rats. Chinese Journal of Integrative Medicine, no. 20400: 1–7.
• Rao, U. S. M. ve Adinew, B., 2011. Remnant B-Cell-Stimulative and Anti-Oxidative Effects of Persea americana Fruit Extract Studied in Rats Introduced into Streptozotocin - Induced Hyperglycaemic State. African Journal of Traditional, Complementary and Alternative Medicines 8 (3): 210–17.
• Roberts, C. G., Gurisik, E., Biden, T. J., Sutherland, R. L. ve Butt, A. J., 2007. Synergistic sytotoxicity between tamoxifen and the plant toxin persin in human breast cancer cells is dependent on Bim expression and mediated by modulation of ceramide metabolism. Molecular Cancer Therapeutics 6 (10): 2777–85.
• Roberts, R. L., Green, J. ve Lewis, B., 2009. Lutein and zeaxanthin in eye and skin health. Clinics in Dermatology 27 (2): 195–201.
• Rodríguez-Carpena, J. G., Morcuende, D., Andrade, M. J., Kylli, P. ve Estevez, M., 2011. Avocado (Persea americana Mill.) Phenolics, In Vitro Antioxidant and Antimicrobial Activities, and Inhibition of Lipid and Protein Oxidation in Porcine Patties. Journal of Agricultural and Food Chemistry 59 (10): 5625–35.
• Rodríguez-García, C., Sánchez-Quesada, C. ve Gaforio, J. J., 2019. Dietary Flavonoids as Cancer Chemopreventive Agents: An Updated Review of Human Studies. Antioxidants 8 (5): 1–23.
• Rodriguez-Sanchez, D. G., Flores-García, M., Silva-Platas, C., Rizzo, S., Torre-Amione, G., Peña-Diaz, A. D. L., Hernández-Brenes, C. ve García-Rivas, G., 2014. Isolation and chemical identification of lipid derivatives from avocado (Persea americana) pulp with antiplatelet and antithrombotic activities. Food and Function 6 (1): 193–203.
• Rodríguez-Sánchez, D., Silva-Platas, C., Rojo, R. P., García, N., Cisneros-Zevallos, L., García-Rivas, G. ve Hernández-Brenes, C., 2013. Activity-guided identification of acetogenins as novel lipophilic antioxidants present in avocado pulp (Persea americana). Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 942–943: 37–45.
• Rosenblat, G., Meretski, S., Segal, J., Tarshis, M., Schroeder, A., Zanin-Zhorov, A., Lion, G., Ingber, A. ve Hochberg, M., 2011. Polyhydroxylated fatty alcohols derived from avocado suppress inflammatory response and provide non-sunscreen protection against UV-induced damage in skin cells. Archives of Dermatological Research 303 (4): 239–46.
• Rotta, E. M., Morais, D. R. D., Biondo, P. B. F., Santos, V. J. D., Matsushita, M. ve Visentainer, J. V., 2016. Use of avocado peel (Persea americana) in tea formulation: a functional product containing phenolic compounds with antioxidant activity. Acta Scientiarum - Technology 38 (1): 23–29.
• Salgado, J. M., Bin, C., Mansi, D. N. ve Souza, A., 2008. Effect of the hass avocado (Persea american Mill) on hipercolesterolemic rats. Ciência e Tecnologia de Alimentos 28 (4): 922–28.
• Salonen, R. M., Nyyssönen, K., Kaikkonen, J., Porkkala-Sarataho, E., Voutilainen, S., Rissanen, T. H., Tuomainen, T. P., Valkonen, V. P., Ristonmaa, U., … ve Lakka, H. M., 2003. Six-Year Effect of Combined Vitamin C and E Supplementation on Atherosclerotic Progression: The Antioxidant Supplementation in Atherosclerosis Prevention (ASAP) Study. Circulation 107 (7): 947–53.
• Savaş, N., Öğüt, S., Olgun, A. ve Güvenç, A., 2019. Avokadonun (Persea americana) Caenorhabditis elegans Termotoleransı Üzerine Etkilerinin İncelenmesi. Adnan Menderes Üniversitesi Sağlık Bilimleri Fakültesi Dergisi 4 (1): 30–36.
• Segovia, F. J., Corral-Pérez, J. J. ve Almajano, M. P., 2016. Avocado Seed: Modeling extraction of bioactive compounds.” Industrial Crops and Products 85: 213–20.
• Serra-Majem, L., Roman, B. ve Estruch, R., 2006. Scientific Evidence of Interventions Using the Mediterranean Diet: A Systematic Review. Nutrition Reviews 64 (2): 27-47.
• SIAP, 2015. Servicio de Información Agroalimentaria y Pesquera. http://infosiap.siap.gob. mx/aagricola_siap_gb/ientidad/index.jsp, Accessed date: 21 August 2017.
• Sıcak, Y., Çolak, Ö. F., İlhan, V., Sevindik, E. ve Alkan, N., 2013. Köyceğiz Yöresinde Halk Arasında Yaygın Olarak Kullanılan Bazı Tıbbi ve Aromatik Bitkiler. Journal of Anatolian Natural Sciences 4(2): 70-77.
• Soong, Y. Y. ve Barlow, P. J., 2004. Antioxidant activity and phenolic content of selected fruit seeds. Food Chemistry 88 (3): 411–17. Tan, C. X., 2019. Virgin avocado oil: An emerging source of functional fruit oil. Journal of Functional Foods 54 (October 2018): 381–92.
• TUIK, 2019. http://www.tuik.gov.tr (17.12.2019).
• Tremocoldi, M. A., Rosalen, P. L., Franchin, M., Massarioli, A. P., Denny, C., Daiuto, E. R., Paschoal, J. A. R., Melo, P. S. ve Alencar, S. M. D., 2018. Exploration of Avocado By-Products as Natural Sources of Bioactive Compounds. PLOS ONE.
• Uçak, S. ve Kızıltan, G., 2021. Akdeniz Diyeti ve Kanser. Başkent Üniversitesi Sağlık Bilimleri Fakültesi Dergisi 6 (2): 105–21.
• Utrera, M., Rodríguez-Carpena, J. G., Morcuende, D. ve Estévez, M., 2012. Formation of Lysine-Derived Oxidation Products and Loss of Tryptophan during Processing of Porcine Patties with Added Avocado Byproducts. Journal of Agricultural and Food Chemistry 60 (15): 3917–26.
• Vermerris, W. ve Nicholson, R., 2006. Families of phenolic compounds and means of classification: Phenolic compound biochemistry. Editörler: Vermerris, W., Nicholson, R., Springer, Netherlands, pp: 1-34.
• Vinha, A. F., Sousa, C., Soares, M. O. ve Barreira, S. V. P., 2020. Avocado and Its By-Products : Natural Sources of Nutrients, Phytochemical Compounds and Functional Properties. Agricultural and Food Science, 1, 82-96.
• Voutilainen, S., Nurmi, T., Mursu, J. ve Rissanen, T. H., 2006. Carotenoids and cardiovascular health. American Journal of Clinical Nutrition 83 (6): 1265–71.
• Wang, W., Bostic, T. R. ve Gu, L., 2010. Antioxidant capacities, procyanidins and pigments in avocados of different strains and cultivars. Food Chemistry 122 (4): 1193–98.
• Weremfo, A., Adulley, F. ve Adarkwah-Yiadom, M., 2020. Simultaneous Optimization of Microwave-Assisted Extraction of Phenolic Compounds and Antioxidant Activity of Avocado (Persea americana Mill.) Seeds Using Response Surface Methodology. Journal of Analytical Methods in Chemistry 2020.
• Widiyastuti, Y., Pratiwi, R., Riyanto, S. ve Wahyuono, S., 2018. Cytotoxic activity and apoptosis induction of avocado Persea americana Mill. seed extract on MCF-7 cancer cell line. Indonesian Journal of Biotechnology 23 (2): 61–67.
• WHO, 2017. World Health Organization, Cardiovascular Diseases. Son Erişim: Available at: http://www.who.int/ cardiovascular_diseases/en/ Accessed July 08, 2017.
• Yamassaki, F. T., Campestrini, L. H., Zawadzki-Baggio, S. F. ve Maurer, J. B. B., 2017. Avocado leaves: Influence of drying process, thermal incubation, and storage conditions on preservation of polyphenolic compounds and antioxidant activity. International Journal of Food Properties 20 (2): 2280–93.
• Yasir, M., Das, S. ve Kharya, M., 2010. The phytochemical and pharmacological profile of Persea americana Mill. Pharmacognosy Reviews 4 (7): 77–84.
• Yurt, M. ve Büyüktuncer Demirel, Z., 2017. Sağlıklı Beslenmede Avokadonun Yeri. Bes Diy Derg 45 (2): 161–70.
• Zafar, T. ve Sidhu, J. S., 2011. Avocado: Production, Quality, and Major Processed Products. Handbook of Vegetables and Vegetable Processing 1871: 525–43.
• Zakariya, U. A., Umar, U. A., Dambazau, S. M. ve Sulaiman, A., 2016. Comparative Hepatotoxic Effects of Aqueous and Phenolic Extracts of Avocado (Persea americana) Seed in Wistar Albino Rats. International Journal of Biochemistry Research & Review 10 (4): 1–6.