The Journal of Faculty of Pharmacy of Ankara University is an international medium, an open access, peer-reviewed journal for the publication of original research reports, reviews and short communications in English or Turkish on relevant developments in pharmaceutical sciences., Proceeding of scientific meetings may be published as special issues of supplements to the journal. Additionally, it contains previous and forthcoming national and international scientific meetings with social activities in pharmaceutical areas.
Research Article
Year 2019,
Volume: 43 Issue: 3, 230 - 238, 08.09.2019
Abstract
Objective: World's ageing population continues to grow older
together with high percentage prevalence of Alzheimer's disease encourage
science to find herbs or biologic compounds which can be utilized for it
prophylaxis. Juglans regia L. is not simply an agricultural product, its
fruits, leaves, barks, stems, pericarps, and flowers are all utilized for
diversified medicinal usages. In the present investigation, we examined the
anticholinesterase and antioxidant capacities of the lyophilized aqueous
extracts of leaves from J. regia.
Material and Method: Phenolic contents and antioxidant
activity were assessed by Folin-Ciocalteu's, qualitative/quantitative DPPH and
TBA methods. Anticholinesterase activity was assessed by Ellman’s method.
Result and Discussion: The total phenolic content was
found 478.32 mg/g. The extract indicated antioxidant activity with DPPH test
(IC50: 123.66 μg/mL) and
TBA assay (IC50: 209.23 μg/mL).
Moreover, the extract of J. regia indicated remarkable inhibition towards
acetylcholinesterase (40.09%) and butyrylcholinesyterase (56.23%) enzymes.
These data propose that J. regia can be utilised a potency for pharmaceutical
products which get antioxidant and anticholinesterase activity.
References
- 1. Alzheimer’s Association. Alzheimer’s disease facts and figures. Alzheimer's & Dementia 2018, 14, 367–429.
- 2. Karch, S.; Broichhagen, J.; Schneider, J.; Böning, D.; Hartmann, S.; Schmid, B.; Tripa, P.; Palmisano, R.; Alzheimer, C.; Johnsson, K.; Huth, T. A new fluorogenic small-molecule labeling tool for surface diffusion analysis and advanced fluorescence imaging of β‑site amyloid precursor protein-cleaving enzyme 1 based on silicone rhodamine: SiR-BACE1. J Med Chem 2018, 61, 6121–6139.
- 3. Ustun, O.; Senol, F.S.; Kurkcuoglu, M.; Orhan, I.E.; Kartal, M.; Baser, K.H.C. Investigation on chemical composition, anticholinesterase and antioxidant activities of extracts and essential oils of Turkish Pinus species and pycnogenol. Industrial Crops and Products 2012, 38, 115–123.
- 4. Ferreira, A.; Proenc, C.; Serralheiro, M.L.M.; Araujo, M.E.M. The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. J Ethnopharmacol 2006, 108, 3–37.
- 5. Luz, D.A.; Pinheiro, A.M.; Silva, M.L.; Monteiroa, M.C.; Prediger, R.D.; Maiaa, C.S.F.; Andrad, E; Júniora, F. Ethnobotany, phytochemistry and neuropharmacological effects of Petiveria alliacea L. (Phytolaccaceae): A review. J Ethnopharmacol 2016, 185, 182–201.
- 6. Perry, E.K.; Pickering, A.T.; Wang, W.W.; Houghton, P; Perry, N.L. Medicinal Plants and Alzheimer's Disease: Integrating Ethnobotanical and Contemporary Scientific Evidence. The Journal of Alternative and Complementary Medicine 1998, 4, 419–428.
- 7. Sharafati-Chaleshtori, R.; Sharafati-Chaleshtori F.; Rafieian, M. Biological characterization of Iranian walnut (Juglans regia) leaves. Turk J Biol 2011, 35, 635–639.
- 8. Zhang, Z.; Liao, L.; Moore, J.; Wua, T.; Wang, Z. Antioxidant phenolic compounds from walnut kernels (Juglans regia L.). Food Chemistry 2009, 113(1), 160–165.
- 9. Carvalho, M.; Ferreira, P.J.; Mendes, V.S.; Silva, R.; Pereira, J.A.; Jerónimo, C.; Silva, B.M. Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. Food and Chemical Toxicology 2010, 48(1), 441–447.
- 10. Karakaya, S; Koca, M; Kilic, C.S.; Coskun, M. Antioxidant and anticholinesterase activities of Ferulago syriaca Boiss. and F. isaurica Peșmen growing in Turkey. Med Chem Res 2018, 27, 1843–1850.
- 11. Ellman, G.L.; Courtney, K.D.; Andresjr, V.; Featherstone, R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961, 7, 88–95.
- 12. Yerdelen, K.Ö.; Tosun, A. Synthesis, docking and biological evaluation of oxamide and fumaramide analogs as potential AChE and BuChE inhibitors. Med Chem Res 2015, 24, 588–602.
- 13. Pereira, J.A.; Oliveira, I; Sousa, A.; Valentao, P.; Andrade, P.B.; Ferreira, I.C.F.R.; Ferreres, F.; Bento, A.; Seabra, R.; Estevinho, L. Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. Food and Chemical Toxicology 2007, 45, 2287–2295.
- 14. Almeida, I.F.; Fernandes, E.; Lima, J.L.F.C.; Costa, P.C.; Bahia, M.F. Walnut (Juglans regia) leaf extracts are strong scavengers of pro-oxidant reactive species. Food Chemistry 2008, 106, 1014–1020.
- 15. Amaral, J.S.; Seabra, R.M.; Andrade, P.B.; Valentao, P.; Pereira, J.A.; Ferreres, F. Phenolic profile in the quality control of walnut (Juglans regia L.) leaves. Food Chemistry 2004, 88, 373–379.
- 16. Santos, A.; Barros, L.; Calhelhaa, R.C.; Duenas, M.; Carvalho, A.M.; Santos-Buelga, C.; Ferreira, I.C.F.R. Leaves and decoction of Juglans regia L.: Different performances regarding bioactive compounds and in vitro antioxidant and antitumor effects. Industrial Crops and Products 2013, 51, 430–436.
- 17. Sytar, O.; Bruckova, K.; Hunkova, E.; Zivcak, M.; Kiessoun, K.; Brestic, M. The application of Muliplex flourimetric sensor for analysis flavonoids content in the medical herbs family Asteraceae, Lamiaceae, Rosaceae. Biological research 2015, 48(5):DOI: 10.1186/0717-6287-48-5.
- 18. Granato, D.; Shahidi, F.; Wrolstad, R.; Kilmartin, P.; Melton, L.D.; Hidalgo, F.J.; Miyashita, K.; Camp, J.; Alasalvar, C.; Ismail, A.B.; Elmore, S.; Birch, G.G.; Charalampopoulos, D.; Astley, S.B.; Pegg, R.; Zhou, P.; Finglas, P. Antioxidant activity, total phenolics and flavonoids contents: Should we ban in vitro screening methods?. Food Chem 2018, 264, 471–475.
Year 2019,
Volume: 43 Issue: 3, 230 - 238, 08.09.2019
Abstract
Amaç: Dünyanın
yaşlanan nüfusu, Alzheimer hastalığının yüksek oranda prevalansı ile birlikte
yaşlanmaya devam etmekte ve bilimi, profilakside kullanılabilecek bitkileri
veya biyolojik bileşikleri bulmaya teşvik etmektedir. Juglans regia L. basit
tarımsal bir ürün değildir, meyvelerinin, yapraklarının, kabuklarının,
gövdelerinin, perikarplarının ve çiçeklerinin farklı tıbbi kullanım alanları
bulunmaktadır. Bu çalışmada, J. regia'nın yapraklarının liyofilize sulu
ekstrelerinin antikolinesteraz ve antioksidan kapasitelerini inceledik.
Gereç ve Yöntem: Fenolik
içerik ve antioksidan aktivite Folin-Ciocalteu, kalitatif/kantitatif DPPH ve
TBA yöntemleri ile belirlenmiştir. Antikolinesteraz aktivite ise Ellman metodu
ile değerlendirilmiştir.
Sonuç ve Tartışma: Total fenolik içerik 478.32 mg/g olarak
bulunmuştur. Örnekler DPPH (123.66 μg/mL) ve TBA (209.23 μg/mL) testleri ile
antioksidan aktivite göstermiştir. Ayrıca, ceviz ekstresi, sırasıyla
asetilkolinesteraz (%40.09) ve butirilkolinesteraz (%56.23) enzimlerine karşı
önemli ölçüde inhibisyon göstermiştir. Bu veriler, J. regia'nın, antioksidan ve
antikolinesteraz aktivitesi alan farmasötik ürünler için bir potansiyel olarak
kullanabileceğini önermektedir.
References
- 1. Alzheimer’s Association. Alzheimer’s disease facts and figures. Alzheimer's & Dementia 2018, 14, 367–429.
- 2. Karch, S.; Broichhagen, J.; Schneider, J.; Böning, D.; Hartmann, S.; Schmid, B.; Tripa, P.; Palmisano, R.; Alzheimer, C.; Johnsson, K.; Huth, T. A new fluorogenic small-molecule labeling tool for surface diffusion analysis and advanced fluorescence imaging of β‑site amyloid precursor protein-cleaving enzyme 1 based on silicone rhodamine: SiR-BACE1. J Med Chem 2018, 61, 6121–6139.
- 3. Ustun, O.; Senol, F.S.; Kurkcuoglu, M.; Orhan, I.E.; Kartal, M.; Baser, K.H.C. Investigation on chemical composition, anticholinesterase and antioxidant activities of extracts and essential oils of Turkish Pinus species and pycnogenol. Industrial Crops and Products 2012, 38, 115–123.
- 4. Ferreira, A.; Proenc, C.; Serralheiro, M.L.M.; Araujo, M.E.M. The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. J Ethnopharmacol 2006, 108, 3–37.
- 5. Luz, D.A.; Pinheiro, A.M.; Silva, M.L.; Monteiroa, M.C.; Prediger, R.D.; Maiaa, C.S.F.; Andrad, E; Júniora, F. Ethnobotany, phytochemistry and neuropharmacological effects of Petiveria alliacea L. (Phytolaccaceae): A review. J Ethnopharmacol 2016, 185, 182–201.
- 6. Perry, E.K.; Pickering, A.T.; Wang, W.W.; Houghton, P; Perry, N.L. Medicinal Plants and Alzheimer's Disease: Integrating Ethnobotanical and Contemporary Scientific Evidence. The Journal of Alternative and Complementary Medicine 1998, 4, 419–428.
- 7. Sharafati-Chaleshtori, R.; Sharafati-Chaleshtori F.; Rafieian, M. Biological characterization of Iranian walnut (Juglans regia) leaves. Turk J Biol 2011, 35, 635–639.
- 8. Zhang, Z.; Liao, L.; Moore, J.; Wua, T.; Wang, Z. Antioxidant phenolic compounds from walnut kernels (Juglans regia L.). Food Chemistry 2009, 113(1), 160–165.
- 9. Carvalho, M.; Ferreira, P.J.; Mendes, V.S.; Silva, R.; Pereira, J.A.; Jerónimo, C.; Silva, B.M. Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. Food and Chemical Toxicology 2010, 48(1), 441–447.
- 10. Karakaya, S; Koca, M; Kilic, C.S.; Coskun, M. Antioxidant and anticholinesterase activities of Ferulago syriaca Boiss. and F. isaurica Peșmen growing in Turkey. Med Chem Res 2018, 27, 1843–1850.
- 11. Ellman, G.L.; Courtney, K.D.; Andresjr, V.; Featherstone, R.M. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961, 7, 88–95.
- 12. Yerdelen, K.Ö.; Tosun, A. Synthesis, docking and biological evaluation of oxamide and fumaramide analogs as potential AChE and BuChE inhibitors. Med Chem Res 2015, 24, 588–602.
- 13. Pereira, J.A.; Oliveira, I; Sousa, A.; Valentao, P.; Andrade, P.B.; Ferreira, I.C.F.R.; Ferreres, F.; Bento, A.; Seabra, R.; Estevinho, L. Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. Food and Chemical Toxicology 2007, 45, 2287–2295.
- 14. Almeida, I.F.; Fernandes, E.; Lima, J.L.F.C.; Costa, P.C.; Bahia, M.F. Walnut (Juglans regia) leaf extracts are strong scavengers of pro-oxidant reactive species. Food Chemistry 2008, 106, 1014–1020.
- 15. Amaral, J.S.; Seabra, R.M.; Andrade, P.B.; Valentao, P.; Pereira, J.A.; Ferreres, F. Phenolic profile in the quality control of walnut (Juglans regia L.) leaves. Food Chemistry 2004, 88, 373–379.
- 16. Santos, A.; Barros, L.; Calhelhaa, R.C.; Duenas, M.; Carvalho, A.M.; Santos-Buelga, C.; Ferreira, I.C.F.R. Leaves and decoction of Juglans regia L.: Different performances regarding bioactive compounds and in vitro antioxidant and antitumor effects. Industrial Crops and Products 2013, 51, 430–436.
- 17. Sytar, O.; Bruckova, K.; Hunkova, E.; Zivcak, M.; Kiessoun, K.; Brestic, M. The application of Muliplex flourimetric sensor for analysis flavonoids content in the medical herbs family Asteraceae, Lamiaceae, Rosaceae. Biological research 2015, 48(5):DOI: 10.1186/0717-6287-48-5.
- 18. Granato, D.; Shahidi, F.; Wrolstad, R.; Kilmartin, P.; Melton, L.D.; Hidalgo, F.J.; Miyashita, K.; Camp, J.; Alasalvar, C.; Ismail, A.B.; Elmore, S.; Birch, G.G.; Charalampopoulos, D.; Astley, S.B.; Pegg, R.; Zhou, P.; Finglas, P. Antioxidant activity, total phenolics and flavonoids contents: Should we ban in vitro screening methods?. Food Chem 2018, 264, 471–475.
There are 18 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Pharmacology and Pharmaceutical Sciences |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 8, 2019 |
| Submission Date | February 21, 2019 |
| Acceptance Date | July 2, 2019 |
| Published in Issue | Year 2019 Volume: 43 Issue: 3 |