APOPTOTIC AND CYTOTOXIC EFFECT OF ORIGANUM MINUTIFLORUM ON NB2A NEUROBLASTOMA CELLS

Year 2020, Volume: 44 Issue: 2, 281 - 292, 31.05.2020

İsmail Sari , Gonca Dönmez , Oktay Özkan , İşil Aydemir , Caner Özbey Mehmet İbrahim Tuğlu

https://doi.org/10.33483/jfpau.707277

Abstract

Objective: The aim of the present study is to investigate the cytotoxic and apoptotic effects of oregano oil from Origanum minutiflorum, which is an endemic medicinal plant in Turkey, on NB2a neuroblastoma cells.

Material and Method: Cell proliferation, apoptosis, and expression of inducible and endothelial nitric oxide synthase have been determined by MTT, TUNEL, and immunohistochemistry, respectively.

Result and Discussion: The cell viability was significantly decreased gradually in NB2a cells when the concentrations of both oregano oil and doxorubicin were increased. IC50 doses were found to be10.75 µl/ml for oregano oil and 5µM for doxorubicin. Furthermore, expression of inducible and endothelial nitric oxide synthase, also the number of apoptotic cells was significantly enhanced in NB2a cells after treatment with oregano oil and doxorubicin. There was a remarkable increase in inducible and endothelial nitric oxide synthase expression levels and in the number of apoptotic cells by the dual application of these agents. In addition, oregano oil enhanced the apoptotic effect of doxorubicin. In conclusion, our results indicated that oregano oil of O. minutiflorum has a cytotoxic and apoptotic effect on NB2a cells and these effects may be related to its enhancing effects on inducible and endothelial nitric oxide synthase levels.

Keywords

apoptosis, cytotoxicity, neuroblastoma, nitric oxide synthase, Origanum minutiflorum

Supporting Institution

No funding was received.

Thanks

The authors wish to thank Associate Prof. Dr. Sabri Erbaş, Agriculture Faculty, Suleyman Demirel University, Isparta, Turkey, for providing the plant material. The abstract of this work has been previously presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy and published in the Multidisciplinary Digital Publishing Institute Proceedings Journal, vol.1 number 10 (https://www.mdpi.com/2504-3900/1/10/1026).

ORIGANUM MINUTIFLORUM'UN NB2A NÖROBLASTOMA HÜCRELERİNE APOPTOTİK VE SİTOTOKSİK ETKİSİ

Abstract

Amaç: Bu çalışmanın amacı, Türkiye'de endemik bir tıbbi bitki olan Origanum minutiflorum'dan elde edien Oregano yağının NB2a nöroblastoma hücrelerine sitotoksik ve apoptotik etkilerini incelemektir.

Gereç ve Yöntem: Hücre proliferasyonu, apoptoz, İndüklenebilir ve endotelyal nitrik oksit sentazın ekspresyonu sırasıyla MTT, TUNEL ve immünohistokimya ile belirlendi.

Sonuç ve Tartışma: Hem Oregano yağı hem de doksorubisin derişimleri artırıldığında NB2a hücrelerinin canlılığı kademeli olarak azaldı. IC50 dozu Oregano yağı için 10.75 ul / ml doksorubisin için 5µM olarak belirlendi. Ayrıca, Oregano yağı ve doksorubisin ile muamele edildikten sonra NB2a hücrelerinde indüklenebilir ve endotelyal nitrik oksit sentazın ekspresyonu ve apoptotik hücre sayısı anlamlı olarak yükseldi. Bu ajanların ikili uygulaması ile indüklenebilir ve endotelyal nitrik oksit sentaz ekspresyon seviyelerinde ve apoptotik hücre sayısında dikkate değer bir artış olmuştur. Ek olarak, Oregano yağı, doksorubisinin apoptotik etkisini arttırmıştır. Sonuç olarak, bulgularımız O. minutiflorum’a ait Oregano yağının NB2a hücreleri üzerinde sitotoksik ve apoptotik bir etkiye sahip olduğuna ve bu etkilerin Oregano yağının indüklenebilir ve endotelyal nitrik oksit sentaz düzeylerini arttırıcı etkileri ile ilişkili olabileceğine işaret etmektedir.

Keywords

apoptoz, sitotoksisite, nöroblastom, nitrik oksit sentaz, Origanum minutiflorum

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