DETERMINATION OF DNA DAMAGE INDUCED BY BISPHENOL A AND BISPHENOL S IN MCF7 CELL LINE

Year 2024, Volume: 48 Issue: 2, 552 - 558, 20.05.2024

Ekin Erdoğmuş , Seda Ipek Tekneci , Belma Gümüşel , Yalçın Duydu , Aylin Üstündağ

https://doi.org/10.33483/jfpau.1428739

Abstract

Objective: This study aimed to determine the DNA damage induced by Bisphenol A (BPA) and Bisphenol S (BPS) on MCF7 cell line.
Material and Method: DNA damage was determined by COMET assay in MCF7 cell line at 0.1, 0.5, 1, 5, 10 and 50 μM concentrations of BPA and BPS.
Result and Discussion: All BPA and BPS concentrations studied (0.1, 0.5, 1, 5, 10 and 50 μM) significantly induced DNA damage on MCF7 cell line compared with control (p<0.05). BPS significantly induced DNA damage more than BPA at the 3 highest concentrations studied (5, 10 and 50 μM) (p<0.05). This study shows that bisphenol derivatives can also cause DNA damage like BPA.

Keywords

Bisphenol A, bisphenol S, COMET assay, DNA damage, MCF7 cell line

Ethical Statement

The authors declare that ethics committee approval is not mandatory for this study.

Supporting Institution

Ankara University Scientific Research Projects Coordination Unit

Project Number

TDK-2022-2702

BİSFENOL A VE BİSFENOL S’NİN MCF7 HÜCRE HATTINDA NEDEN OLDUĞU DNA HASARININ ARAŞTIRILMASI

Abstract

Amaç: Bu çalışmada, Bisfenol A (BPA) ve Bisfenol S (BPS)'nin MCF7 hücre hattında neden olduğu DNA hasarının belirlenmesi amaçlanmıştır.
Gereç ve Yöntem: DNA hasarı BPA ve BPS’nin 0.1, 0.5, 1, 5, 10 ve 50 μM konsantrasyonlarda MCF7 hücre hattında COMET yöntemi ile belirlenmiştir.
Sonuç ve Tartışma: Çalışılan tüm BPA ve BPS konsantrasyonları (0.1, 0.5, 1, 5, 10 ve 50 μM) MCF7 hücre hattında kontrole kıyasla önemli ölçüde DNA hasarına neden olmuştur (p<0.05). BPS, çalışılan en yüksek 3 konsantrasyonda (5, 10 ve 50 μM) DNA hasarını BPA'dan daha fazla indüklemiştir (p<0.05). Bu çalışma bisfenol türevlerinin de BPA gibi DNA hasarına neden olabileceğini göstermektedir.

Keywords

Bisfenol A, bisfenol S, COMET testi, DNA hasarı, MCF7 hücre hattı

Project Number

TDK-2022-2702

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