Bitki Gelişimini Tetikleyen Rizobakterilerin Uygulandığı Buğdayda (Triticum aestivum L.) Kuraklık Stresi ile İlişkili Bazı Genlerin İfade Seviyesinin Ölçülmesi

Year 2021, Volume: 11 Issue: 3, 2357 - 2370, 01.09.2021

Behcet İnal , Harun Bektaş , Mohsen Mırzapour , Serdar Altıntaş , Fatih Çığ , Mustafa Cengiz , Mehmet Sonkurt

https://doi.org/10.21597/jist.890272

Abstract

Ekmeklik buğday (Triticum aestivum L.) temel besin kaynağı olması ve tüm dünyada üretimi yapılabilen bir ürün olması nedeniyle sürdürülebilir tarım açısından en önemli bitki türlerinden biridir. Buğday bitkisinin genetik yapı olarak mısır, çeltik ve diğer tüm tarımsal ürünlerden daha kompleks bir yapıya sahip olması bu türün ıslahını zor ve uzun zaman alan bir süreç haline getirmektedir. Diğer taraftan verim değerlerinin istenilen noktalara getirilebilmesi buğdayın çevresel faktörlere verdiği tepkilerin anlaşılması ile mümkün olabilmektedir. Bu çalışmada da buğday ıslahında en sık karşılaşılan sorunlardan kuraklık ve hastalıklara karşı direncin ACC deaminaz etkisi gösteren PGPB (Plant Growth Promoting Bacteria) ile ilişkisi incelenmiştir. Çalışmamızda ACC deaminaz sentezleyen bakterilerin iki farklı ekmeklik buğday çeşidinde (Gerek 79, Bezostaja 1) ve kuraklık koşullarındaki etkisi incelenmiştir. Çalışma ile ACC deaminaz etkisi ile kuraklığa karşı dayanıklılık mekanizmasında rol alan bazı transkripsiyon faktörlerin ifade seviyeleri q-RT PCR ile ölçülmüştür. Ayrıca her
iki buğday genotipinde glutatyon redüktaz seviyesi ölçülerek genler ile olan ilişkisi ortaya konulmuştur. Çalışma sonucunda elde edilen veriler değişen etkinlik derecesine sahip olmakla birlikte PGPB bakterilerinin kuraklık stresinin olumsuz etkilerini azaltıcı etkiye sahip olduğu bulunmuştur.

Keywords

Kuraklık, buğday, ACC deaminaz, gen ifadesi, transkripsiyon faktör, glutatyon

Supporting Institution

Siirt Üniversitesi Bilimsel Araştırmalar Proje Birimi

Project Number

2017-SİÜZİR-63

Thanks

Bu çalışma Siirt Üniversitesi Bilimsel Araştımalar Proje (BAP) birimi tarafından 2017-SİÜZİR-63 nolu proje kapsamında desteklenmiştir.

Quantification of The Expression Level of Some Drought Stress-Related Genes in Wheat (Triticum aestivum L.) Treated With Plant Growth Promoting Rhizobacteria

Abstract

Bread wheat (Triticum aestivum L.) is one of the most important plant species in terms of sustainable
agriculture, as it is a basic food source and a product that can be produced all over the world. The fact that the wheat plant has a more complex genetic structure than corn, paddy and all other agricultural products makes the breeding of this species a difficult and time-consuming process. On the other hand, it is possible to bring the yield values to the desired points by understanding the reactions of wheat to environmental factors. In this study, the relationship of resistance to drought and diseases, which are the most common problems in wheat breeding, with PGPB (Plant Growth Promoting Bacteria), which has ACC deaminase effect, was investigated. In our study, the effect of ACC deaminase-synthesizing bacteria on two
different bread wheat varieties (Need 79, Bezostaja 1) and in drought conditions was investigated. In this study, expression levels of some transcription factors involved in drought resistance mechanism with ACC deaminase effect were measured by q-RT PCR. In addition, the glutathione reductase level was measured in both wheat genotypes and its relationship with the genes was revealed. Although the data obtained as a result of the study have varying degrees of activity, it has been found that PGPB bacteria have a reducing effect on the negative effects of drought stress.

Keywords

Rhizobacteria, wheat, ACC deaminase, gene expression, transcription factor, glutathion

Project Number

2017-SİÜZİR-63

References

• Agarwal PK, Agarwal P, Reddy M, Sopory SK, 2006. Role of DREB transcription factors in abiotic and biotic stress tolerance in plants. Plant cell reports, 25:1263-1274.
• Ahuja I, de Vos RC, Bones AM, Hall RD, 2010. Plant molecular stress responses face climate change. Trends in plant science, 15:664-674.
• Alpaslan M, Gunes A, Inal A, 1998. Deneme Teknigi Ankara Universitesi Ziraat Fakultesi Yayinlari: 1501, Ders Kitabi, 423.
• Anbazhagan K, Bhatnagar-Mathur P, Vadez V, Dumbala SR, Kishor PK, Sharma KK, 2015. DREB1A overexpression in transgenic chickpea alters key traits influencing plant water budget across water regimes. Plant cell reports, 34:199-210.
• AYRANCI R, Bayram S, Soylu S, 2017. Ekmeklik buğday genotiplerinin verim ve fenolojik özelliklerinin tane doldurma dönemindeki kuraklık stresine tepkileri. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 26:112-118.
• Banerjee A, Roychoudhury A. 2017. Effect of salinity stress on growth and physiology of medicinal plants. In: Medicinal Plants and Environmental Challenges: Springer. p 177-188.
• Banerjee A, Roychoudhury A, 2018a. Abiotic stress, generation of reactive oxygen species, and their consequences: an overview. Revisiting the role of reactive oxygen species (ROS) in plants: ROS Boon or bane for plants:23-50.
• Banerjee A, Roychoudhury A, 2018b. The gymnastics of epigenomics in rice. Plant cell reports, 37:25-49.
• Basak BB, Biswas DR, 2010. Co-inoculation of potassium solubilizing and nitrogen fixing bacteria on solubilization of waste mica and their effect on growth promotion and nutrient acquisition by a forage crop. Biology and Fertility of Soils, 46:641-648.
• Belimov AA, Safronova VI, Sergeyeva TA, Egorova TN, Matveyeva VA, Tsyganov VE, Borisov AY, Tikhonovich IA, Kluge C, Preisfeld A, 2001. Characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase. Canadian Journal of Microbiology, 47:642-652.
• Berendsen RL, Vismans G, Yu K, Song Y, de Jonge R, Burgman WP, Burmølle M, Herschend J, Bakker PA, Pieterse CM, 2018. Disease-induced assemblage of a plant-beneficial bacterial consortium. The ISME journal, 12:1496-1507.
• Borrill P, Harrington SA, Uauy C, 2017. Genome-wide sequence and expression analysis of the NAC transcription factor family in polyploid wheat. G3: Genes, Genomes, Genetics, 7:3019-3029.
• Cakmak I, Marschner H, 1992. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant physiology, 98:1222-1227.
• Chang H-C, Tsai M-C, Wu S-S, Chang F, 2019. Regulation of ABI5 expression by ABF3 during salt stress responses in Arabidopsis thaliana. Botanical studies, 60:1-14.
• Chen J, Nolan TM, Ye H, Zhang M, Tong H, Xin P, Chu J, Chu C, Li Z, Yin Y, 2017. Arabidopsis WRKY46, WRKY54, and WRKY70 transcription factors are involved in brassinosteroid-regulated plant growth and drought responses. The Plant Cell, 29:1425-1439.
• Clark FE, 1965. Aerobic Spore‐Forming Bacteria. Methods of Soil Analysis: Part 2 Chemical and Microbiological Properties, 9:1473-1476.
• Çekiç CY, Güneş ATD, Kurağa Dayanikli Buğday (Triticum Aestivum L.) Islahinda Seleksiyon Kriteri Olabilecek Fizyolojik Parametrelerin Araştirilmasi. In: Ankara Üniversitesi Fen Bilimleri Enstitüsü Toprak Anabilim Dali.
• Diaz RJ, Rosenberg R, 2008. Spreading dead zones and consequences for marine ecosystems. Science, 321:926-929.
• Ding ZJ, Yan JY, Xu XY, Yu DQ, Li GX, Zhang SQ, Zheng SJ, 2014. Transcription factor WRKY 46 regulates osmotic stress responses and stomatal movement independently in A rabidopsis. The Plant Journal, 79:13-27.
• Etesami H, 2018. Can interaction between silicon and plant growth promoting rhizobacteria benefit in alleviating abiotic and biotic stresses in crop plants?. Agriculture, Ecosystems & Environment, 253:98-112.
• Eulgem T, Rushton PJ, Robatzek S, Somssich IE, 2000. The WRKY superfamily of plant transcription factors. Trends in Plant Science, 5:199-206.
• Franco-Zorrilla JM, López-Vidriero I, Carrasco JL, Godoy M, Vera P, Solano R, 2014. DNA-binding specificities of plant transcription factors and their potential to define target genes. Proceedings of the National Academy of Sciences, 111:2367-2372.
• Gaguancela OA, Zúñiga LP, Arias AV, Halterman D, Flores FJ, Johansen IE, Wang A, Yamaji Y, Verchot J, 2016. The IRE1/bZIP60 pathway and bax inhibitor 1 suppress systemic accumulation of potyviruses and potexviruses in Arabidopsis and Nicotiana benthamiana plants. Molecular plant-microbe interactions, 29:750-766.
• Geda C, Repalli S, Dash G, Swain P, Rao G, 2019. Enhancement of Drought Tolerance in Rice through Introgression of Arabidopsis DREB1A through Transgenic Approach. Journal of Rice Research and Developments, 7:2.
• Glick B, Li J, Shah S, Penrose D, Moffatt B. 1999. ACC deaminase is central to the functioning of plant growth promoting rhizobacteria. In: Biology and Biotechnology of the Plant Hormone Ethylene II: Springer. p 293-298.
• Golldack D, Lüking I, Yang O, 2011. Plant tolerance to drought and salinity: stress regulating transcription factors and their functional significance in the cellular transcriptional network. Plant Cell Reports, 30:1383-1391.
• Gouda S, Kerry RG, Das G, Paramithiotis S, Shin H-S, Patra JK, 2018. Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture. Microbiological Research, 206:131-140.
• He M, Dijkstra FA, 2014. Drought effect on plant nitrogen and phosphorus: a meta‐analysis. New Phytologist, 204:924-931.
• Hobo T, Kowyama Y, Hattori T, 1999. A bZIP factor, TRAB1, interacts with VP1 and mediates abscisic acid-induced transcription. Proceedings of the national academy of sciences, 96:15348-15353.
• Jiang J, Ma S, Ye N, Jiang M, Cao J, Zhang J, 2017. WRKY transcription factors in plant responses to stresses. Journal of Integrative Plant Biology, 59:86-101.
• Jofuku KD, Den Boer B, Van Montagu M, Okamuro JK, 1994. Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. The Plant Cell, 6:1211-1225.
• Johnson RR, Wagner RL, Verhey SD, Walker-Simmons MK, 2002. The abscisic acid-responsive kinase PKABA1 interacts with a seed-specific abscisic acid response element-binding factor, TaABF, and phosphorylates TaABF peptide sequences. Plant Physiology, 130:837-846.
• Liu Q, Kasuga M, Sakuma Y, Abe H, Miura S, Yamaguchi-Shinozaki K, Shinozaki K, 1998. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought-and low-temperature-responsive gene expression, respectively, in Arabidopsis. The Plant Cell, 10:1391-1406.
• Ngumbi E, Kloepper J, 2016. Bacterial-mediated drought tolerance: current and future prospects. Applied Soil Ecology, 105:109-125.
• Niu X, Song L, Xiao Y, Ge W, 2018. Drought-tolerant plant growth-promoting rhizobacteria associated with foxtail millet in a semi-arid agroecosystem and their potential in alleviating drought stress. Frontiers in microbiology, 8:2580.
• Pang C-H, Wang B-S, 2010. Role of ascorbate peroxidase and glutathione reductase in ascorbate–glutathione cycle and stress tolerance in plants. Ascorbate-Glutathione Pathway and Stress Tolerance in Plants, 91-113.
• Pérez-de-Luque A, Tille S, Johnson I, Pascual-Pardo D, Ton J, Cameron DD, 2017. The interactive effects of arbuscular mycorrhiza and plant growth-promoting rhizobacteria synergistically enhance host plant defences against pathogens. Scientific Reports, 7:1-10.
• Qin Y, Tian Y, Liu X, 2015. A wheat salinity-induced WRKY transcription factor TaWRKY93 confers multiple abiotic stress tolerance in Arabidopsis thaliana. Biochemical and Biophysical Research Communications, 464:428-433.
• Qiu Y, Yu D, 2009. Over-expression of the stress-induced OsWRKY45 enhances disease resistance and drought tolerance in Arabidopsis. Environmental and Experimental Botany, 65:35-47.
• Rouphael Y, Cardarelli M, Schwarz D, Franken P, Colla G, Aroca R, 2012. Plant responses to drought stress, Plant Responses to Drought: From Morphological to Molecular Features Berlin (Germany): Springer:171-198.
• Saidi MN, Mergby D, Brini F, 2017. Identification and expression analysis of the NAC transcription factor family in durum wheat (Triticum turgidum L. ssp. durum). Plant Physiology and Biochemistry, 112:117-128.
• Sakuma Y, Maruyama K, Qin F, Osakabe Y, Shinozaki K, Yamaguchi-Shinozaki K, 2006. Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. Proceedings of the National Academy of Sciences, 103:18822-18827.
• Santoyo G, Moreno-Hagelsieb G, del Carmen Orozco-Mosqueda M, Glick BR, 2016. Plant growth-promoting bacterial endophytes. Microbiological Research, 183:92-99.
• Sardans J, Peñuelas J, 2012. The role of plants in the effects of global change on nutrient availability and stoichiometry in the plant-soil system. Plant Physiology, 160:1741-1761.
• Schmitt F-J, Renger G, Friedrich T, Kreslavski VD, Zharmukhamedov SK, Los DA, Kuznetsov VV, Allakhverdiev SI, 2014. Reactive oxygen species: re-evaluation of generation, monitoring and role in stress-signaling in phototrophic organisms. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1837:835-848.
• Shameer S, Prasad T, 2018. Plant growth promoting rhizobacteria for sustainable agricultural practices with special reference to biotic and abiotic stresses. Plant Growth Regulation, 84:603-615.
• Singh M, Awasthi A, Soni SK, Singh R, Verma RK, Kalra A, 2015. Complementarity among plant growth promoting traits in rhizospheric bacterial communities promotes plant growth. Scientific reports, 5:1-8.
• Song Y, Chen L, Zhang L, Yu D, 2010. Overexpression of OsWRKY72 gene interferes in the abscisic acid signal and auxin transport pathway of Arabidopsis. Journal of Biosciences, 35:459-471.
• Untergrasser A, Cutcutache I, Koressaar T, Ye J, Faircloth B, Remm M, Rozen S. 2012. Primer3–new capabilities and interfaces. Nucleic Acids Research. In.
• Verdonck O, Gabriels R, 1992. Reference method for the determination of physical properties of plant substrates. II. Reference method for the determination of chemical properties of plant substrates. Acta Horticulturae, 302:169-179.
• Vurukonda SSKP, Vardharajula S, Shrivastava M, SkZ A, 2016. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research, 184:13-24.
• Wang H, Wang H, Shao H, Tang X, 2016. Recent advances in utilizing transcription factors to improve plant abiotic stress tolerance by transgenic technology. Frontiers in Plant Science, 7:67.
• Wang W, Qiu X, Yang Y, Kim HS, Jia X, Yu H, Kwak S-S, 2019. Sweetpotato bZIP transcription factor IbABF4 confers tolerance to multiple abiotic stresses. Frontiers in Plant Science, 10:630.
• Wang X, Zeng J, Li Y, Rong X, Sun J, Sun T, Li M, Wang L, Feng Y, Chai R, 2015. Expression of TaWRKY44, a wheat WRKY gene, in transgenic tobacco confers multiple abiotic stress tolerances. Frontiers in Plant Science, 6:615.
• Wu X, Shiroto Y, Kishitani S, Ito Y, Toriyama K, 2009a. Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter. Plant Cell Reports, 28:21-30.
• Wu Y, Deng Z, Lai J, Zhang Y, Yang C, Yin B, Zhao Q, Zhang L, Li Y, Yang C, 2009b. Dual function of Arabidopsis ATAF1 in abiotic and biotic stress responses. Cell Research, 19:1279-1290.
• Xue G-P, Way HM, Richardson T, Drenth J, Joyce PA, McIntyre CL, 2011. Overexpression of TaNAC69 leads to enhanced transcript levels of stress up-regulated genes and dehydration tolerance in bread wheat. Molecular Plant, 4:697-712.
• Yao Y, Ni Z, Peng H, Sun F, Xin M, Sunkar R, Zhu J-K, Sun Q, 2010. Non-coding small RNAs responsive to abiotic stress in wheat (Triticum aestivum L.). Functional & Integrative Genomics, 10:187-190.
• Zhang C, Li C, Liu J, Lv Y, Yu C, Li H, Zhao T, Liu B, 2017. The OsABF1 transcription factor improves drought tolerance by activating the transcription of COR413-TM1 in rice. Journal of Experimental Botany, 68:4695-4707.
• Zipfel C, Oldroyd GE, 2017. Plant signalling in symbiosis and immunity. Nature, 543:328-336.