Evaluation of an Edibleİinsect (Locusta migratoria) as a Substrate for Microbial β-fructofuranosidase Production

Year 2023, Volume: 13 Issue: 1, 120 - 129, 01.03.2023

Özden Canlı Taşar , Gani Erhan Taşar

https://doi.org/10.21597/jist.1190049

Abstract

Rising population in the world causes reduction on present food resources. Investigators have
been looking for sustainable, nutritional and alternative food-stocks. Insects have been consumed
as snacks or as food supplement in many countries, but they are still not attractive food resource
worldwide. In the current study, β-fructofuranosidase enzyme production by Galactomyces
geotrichum TS61 (GenBank accession: MN749818) strain was investigated using an edible
insect (Locusta migratoria) as substrate. β-fructofuranosidase is a valuable enzyme in food
industry. Taguchi L16 design of experiment (DOE) was employed to achieve an effective
statistical optimization process, including three factors (concentration of locust powder,
concentration of sucrose and initial pH) with four levels. The optimized conditions were
determined as 40 g/L locust powder, 30 g/L sucrose and 6.0 pH. The analysis of variance results
showed that locust powder had more effect on the enzyme production than sucrose and pH. At
the end of the optimization process, approximately 4-fold higher β-fructofuranosidase production
(40.91 U/mL) was obtained when compared with unoptimized experimental run (9.91 U/mL).
Consequently, powdered insects may serve as an effective supplement for valuable enzyme
production in food industry.

Keywords

Locusta migratoria, insect, β-fructofuranosidase, Taguchi DOE

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