Farklı Ülkelerdeki Loranthus europaeus'un Amino Asit Profili

Year 2023, Volume: 13 Issue: 4, 2743 - 2750, 01.12.2023

Haval Ali , Fikret Karataş , Dursun Özer , Sinan Saydam

https://doi.org/10.21597/jist.1246133

Abstract

Bu çalışmada, farklı ülkelerde yetişen Loranthus europaeus örneklerindeki aminoasit miktarları Yüksek Performanslı Sıvı Kromatografisi (HPLC) ile tayin edildi. Esansiyel olmayan aminoasitler bakımından, İran’da yetişen Loranthus europaeus, aspartik asit, serin, alanin, pirolin ve tirozin; Irak’ta yetişen, glutamik asit, asparajin, glisin, glutamin; Türkiye’de yetişen örneklerin ise sistein yönünden daha zengin olduğu bulunmuştur. Türkiye’de yetişen Loranthus europaeus örneklerinin asparajin, serin, glisin, glutamin, alanine, pirolin ve tirozin; Irakta yetişen aspartik asit, İran’da yetişen ise glutamik asit yönünden fakir oldukları söylenebilir.Toplam esansiyel amino asit açısından en zengin İran iken, en fakir Türkiye’de yetişen Loranthus europaeus örneklerinin olduğu belirlenmiştir. Toplam aminoasit miktarı en fazla Irak örneklerinde iken, en düşük olan ise Türkiye örneklerinde bulunmuştur. Esansiyel ve non-esansiyel aminoasit miktarlarının bölgeler arasında değişiklik göstermesi, coğrafi ve ekolojik farklılıklardan kaynaklandığı söylenebilir.

Keywords

Loranthus europaeus, Amino asitler, Toplam amino asit, HPLC

Amino Acids Profile of Loranthus europaeus from Different Countries

Abstract

In this study, the amounts of amino acids in Loranthus europaeus samples grown in different regions (Türkiye, Iran and Iraq) were analyzed by High Performance Liquid Chromatography (HPLC).The results indicate that, aspartic acid, serine, alanine, proline and tyrosine were found to be highest in samples grown in Iran, glutamic acid, asparagine, glycine, and glutamine were found in Loranthus europaeus samples grown in Iraq, and cysteine was found to be highest in samples grown in Türkiye. It can be said that Loranthus europaeus growing in Turkey is poor in terms of asparagine, serine, glycine, glutamine, alanine, proline and tyrosine, on the other hand aspartic acid found to be lowest in samples from Iraq and glutamic acid is the lowest samples from Iran. It was obtained that while Loranthus europaeus samples grown in Iran is the richest in terms of total essential amino acids, on the other hand samples grown in Türkiye is the poorest. Total amino acids content was highest in Iraqi samples and the lowest in Turkish samples. It can be said that the variation of essential and non-essential amino acids contents between regions could be due to geographical, and ecological differences.

Keywords

Loranthus Europaeus, Amino acids, Total amino acid, HPLC

References

• Aidy A, Bahmani M, Pirhadi M, Kaviar VH, Karimi E, Abbasi N. 2022. Phytochemical Analysis and Antimicrobial Effect of Essential Oil and Extract of Loranthus europaeus Jacq. on Acinetobacter baumannii, Staphylococcus aureus, and Pseudomonas aeruginosa. Kafkas Univ. Vet. Fak. Derg., 28(2): 161–167.
• Al-Fartosy AJM, Al-Rikaby AKJ. 2007. Antioxidant Action of Monoterpene from Loranthus europaeus L. Seeds. Al Basra Journal for Agricultural Science 20: 322-336.
• Azevedo RA, Lea PJ. 2001. Lysine metabolism in higher plants. Amino Acids 20(3): 261-279.
• Bakar B, Çakmak M, Ibrahim MS, Özer D, Saydam S, Karatas F. 2021. Investigation of Amounts of vitamins. lycopene. and elements in the fruits of opuntia ficus-indica subjected to different pretreatments. Biological Trace Element Research 198(1): 315-323.
• Benabderrahim MA, Elfalleh W, Sarikurkcu C, Sarikurkcu RB. 2019. Biological activities and phytochemical composition of organs from Loranthus europaeus,” Ind. Crops Prod., 141: 111772.
• Çakmak M, Özer D, Karataş F, Saydam S. 2021. Combine Effect of Vitamin C and venlafaxine on the Amino Acid Content of Saccharomyces cerevisiae. European Journal of Applied Sciences 9(6): 137-153.
• Duan W, Huang Y, Xiao J, Zhang Y, Tang Y. 2020. Determination of free amino acids, organic acids, and nucleotides in 29 elegant spices. Food Science Nutrition 8(7): 3777–3792.
• Ferenc L, Stefan M, Arben M, Hyssen S. 2014. Handbook of the major forest pests in Southeast Europe, Pristina, pp.71.
• Forde BG, Lea JF, 2007. Glutamate in plants: metabolism, regulation, and signalling. Journal of Experimental Botany 58(9): 2339-2358.
• Griggs P. 1991. Mistletoe, myth, magic and medicine. The Biochemist 13: 3–4
• Haroun SA, Shukry WM, El-Sawy O. 2010. Effect of asparagine or glutamine on growth and metabolic changes in phaseolus vulgaris under in vitro conditions. Bioscience Research 7(1): 1-21.
• Harvala E, Exner J, Becker, H. 1984. Flavonoids of Loranthus europaeus. Journal of Natural Products, 47, 1054-1055.
• Heems D, Luck G, Fraudeau C, Vérette E. 1998. Fully automated precolumn derivatization, on-line dialysis and high- performance liquid chromatographic analysis of amino acids in food, beverages and feedstuff,” J. Chromatogr. A, 798(1–2): 9–17.
• Kalefetoğlu T, Ekmekçi Y. 2005. Bitkilerde kuraklık stresinin etkileri ve dayanıklılık. G.U. G.Ü. Fen Bilimleri Dergisi 18(4): 723-740.
• Kim HJ, Jang SH, Ryu JS, Lee JE, Kim YC, Lee MK, Jang TW, Lee SY, Nakamura H, Nishikata N, Mori M, Noguchi Y, Miyano H, Lee KY. 2015. The performance of a novel amino acid multivariate index fordetecting lung cancer: A case control study in Korea. Lung Cancer 90: 522–527.
• Lake AD, Novak P, Shipkova P, Aranibar N, Robertson DG, Reily MD, Lehman-McKeeman LD, Vaillancourt RR, Cherrington NJ. 2002. Branched chain amino acid metabolism profiles in progressive human nonalcoholic fatty liver disease. Amino Acids 47(3): 603–615.
• Lee DY, Kim EH. 2019. Therapeutic Effects of Amino Acids in Liver Diseases: Current Studies and Future Perspectives. Journal of Cancer Prevention 24(2): 72-78.
• Mendoza-Cozatl DG, Zhai Z, Jobe TO, Akmakjian GZ, Song WY, Limbo O, Russell MR, Kozlovskyy VI, Martinoia E, Vatamaniuk OK, Russell P, Schroeder JI. 2010. Tonoplast-localized Abc2 transporter mediates phytochelatin accumulation in vacuoles and confers cadmium tolerance. Journal of Biological Chemistry 285: 40416-40426.
• Miflin BJ, Habash DZ. 2002. The role of glutamine synthetase and glutamate dehydrogenase in nitrogen assimilation and possibilities for improvement in the nitrogen utilization of crops. Journal of Experimental Botany 53(370): 979- 987.
• Mukhtar ZG, Özer D, Karataş F, Saydam S. 2022. Amino Acid Contents of Some Eggplant Species Grown in Different Region. Journal of the Institute of Science and Technology 12(2): 857-869.
• Olgun M, Budak Başçiftçi Z, Ayter G, Turan M, Aydın D, Şaban D, Sönmez AC, Koyuncu O. 2016. Potasyum Iyodür Uygulamasının Ekmeklik Buğday Çeşitlerinin Biyokimyasal Özellikleri Üzerine Etkisi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 11(2), 46-60.
• Öztürk YE, Gülümser E, Mut H, Çopur Doğrusöz M, Başaran U. 2020. Ökse Otu (Viscum album L.)’nun Yem Kalitesinin Belirlenmesi Türkiye Tarımsal Araştırmalar Dergisi 7(2): 201-206. doi: 10.19159/tutad.731121.
• Ros R, Bertomeu JM, Krueger S. 2014. Serine in plants: biosynthesis, metabolism, and functions. Trends in Plant Science 19(9): 564-569.
• Sadiq IS, Izuagie T, Shuaibu M, Dogoyaro AI, Garba A, Abubakar S. 2013. The Nutritional Evaluation and Medicinal Value of Date Palm (Phoenix dactylifera). International Journal of Modern Chemistry 4(3): 147-154.
• Sanchez FJ, Manzanares M, de Andres, EF, Tenorio JL, Ayerbe L. 1998. Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crops Research 59(3): 225-235.
• Sharquie KE, Noaimi AA, Saleh BA. 2016. Loranthus europaeus as an Alternative Medicine in Treatment of Acute Cutaneous Lesihmaniasis: Review Article. Journal of Cosmetics, Dermatological Sciences and Applications 6: 24-33.
• Sharquie KE, Noaimi AA, Saleh BM, Sharara ZA, Al-Salam WS. 2017. Topical 40% <i>Loranthus europaeus</i> Ointment as an Alternative Medicine in the Treatment of Acute Cutaneous Leishmaniasis versus Topical 25% Podophyllin Solution. Journal of Cosmetics, Dermatological Sciences and Applications 7(2): 148–163.
• Song Y, Xu C, Kuroki H, Liao YY, Tsunoda M. 2018. Recent trends in analytical methods for the determination of amino acids in biological samples. Journal of Pharmaceutical and Biomedical Analysis, 147: 35–49.
• Stoimenova A, Ivanov K, Obreshkova D, Saso L. 2013. Biotechnology in the production of pharmaceutical industry ingredients: Amino acids. Biotechnology & Biotechnological Equipment 27(2): 3620–3626.
• Wagner ML, Fernandez T, Alvarez E, Ricco RA, Hajos S, Gurni AA. 1996. Micromolecular and macromolecular comparison of Argentina mistletoe (Ligaria cuneifolia) and European mistletoe (Viscum album L.). Acta Farmaceutica Bonaerense 15(2): 99–108.
• Wu G. 2010. Functional amino acids in growth, reproduction, and health. Advances in Nutrition 1: 31-37.
• Wu G, Bazer FW, Burghardt RC, Johnson GA, Kim SW, Knabe DA, Li P, Li X, Mc Knight JR, Satterfield MC, Spencer TE. 2011. Proline and hydroxyproline metabolism: implications for animal and human nutrition. 40(4): 1053–1063.
• Xu JJ, Fang X, Li CY, Yang L, Chen XY. 2020. General and specialized tyrosine metabolism pathways in plants. aBIOTECH 1:97-105.
• Zemanova V, Pavlik M, Pavlikova D. 2017. Cadmium toxicity induced contrasting patterns of concentrations of free sarcosine, specific amino acids and selected microelements in two Noccaea species. Plos One 12(5): 1-17.
• Zhou W, Wang Y, Yang F, Dong Q, Wang H, Hu N. 2019. Rapid Determination of Amino Acids of Nitraria tangutorum Bobr. from the Qinghai-Tibet Plateau Using HPLC-FLD-MS/MS and a Highly Selective and Sensitive Pre-Column Derivatization Method. Molecules 24: 1665.