Antifungal Activity of Extracts From the Ferulago Pauciradiata in Vitro Against Botrytis Cinerea Pers

Year 2023, Volume: 13 Issue: 4, 2467 - 2475, 01.12.2023

Sinem Karakuş , Tuba Nur Suyurdu , Ekrem Köksal , Cemalettin Alp

https://doi.org/10.21597/jist.1325958

Abstract

This is the first study to reveal the antifungal properties of Ferulago pauciradiata plant. In this context investigated the effects of methanol (FPM), ethanol (FPE), hexane (FPH) and water (FPW) extracts of the F. pauciradiata plant on the prevention of losses caused by gray mold (Botrytis cinerea Pers) in vitro. The effects of FPM, FPE, and FPH 10, 25, 50, 100, 300, 500, 1000, and 2000 µL doses were determined by mycelium growth, germ tube elongation, and spore germination in vitro. The water extract didn’t show antifungal activity against B. cinerea. Compared to the control, both FPM and FPH caused 100% inhibition at the dose of 2000 µL by suppressing mycelial growth due to dose increases, while FPE had a 97.3% effect on the same parameter at the dose of 2000 µL. While there was no elongation at the 2000 µL dose of FPM and FPH, there was an elongation of 8.4 µm at the same dose of FPM. In spore germination, 0% germination was observed in FPM and FPH 2000 µL dose, while 17.5% germination was observed in FPE. These results show that F. pauciradiata extracts, which are of biological origin and are not environmentally toxic, are a good alternative for use in the control of B. cinerea.

Keywords

Biocontrol, Antifungal effect, Gray mold, Plant extract

Thanks

The authors give special thanks to Prof. Dr. Ali Kandemir (Erzincan Binali Yıldırım University, Biology Department) for the identification of plant materials

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