Ev Yapımı Alkollü İçeceklerde Etanolün Kalitatif Tespitinde Raman Spektroskopisi ve Temel Bileşenler Analizi

Year 2024, Volume: 14 Issue: 2, 805 - 811, 01.06.2024

Güneş Açıkgöz

https://doi.org/10.21597/jist.1389267

Abstract

Etanol içeren alkollü ve gazlı içeceklerin fazla miktarda etanol tüketimi, muhakeme yeteneğinin bozulmasına, bilinç kaybına, komaya ve hatta ölüme neden olmaktadır. Çalışmada, Türkiye’ye özgü bir alkollü içecek olan Boğma Rakı’daki etanolün Raman Spektroskopisi ve Temel Bileşenler Analizi (PCA) metodu ile kalitatif analizi amaçlanmıştır. Farklı koşullarda üretimi yapıldığı bilinen Boğma Rakı numunelerinde (22 adet) bulunan etanole ait spesifik bantlar, Raman spektroskopisi kullanılarak incelendi. Elde edilen verilere PCA metodunun uygulanması için OriginPro 2022 programı kullanıldı. Çalışmada, 100-1600 1/cm aralığında etanole ait en belirgin bandın 883 1/cm’de olduğu gözlendi. Numunelerin sınıflandırılması amacıyla kullanılan PCA metodu sonucunda iki tane PCs (Principal Components) değeri elde edildi. Toplam PC1 değeri %95.22 ve toplam PC2 değeri ise %4.64 olarak elde edildi ve bu değerlerin toplamının veri setinin toplam varyansının %99.86'sını oluşturduğu görüldü. Elde edilen PC1 ve PC2 değerlerinin numunelerin etanol ile ilgili olduğu saptandı. Alkollü içeceklerin Raman spektroskopisi incelenmesinin ve elde edilen verilere kemometrik metotların uygulanması, alkollü içeceklerde bulunan etanolün tespitinde etkili olduğu görüldü. Çalışmada, Raman spektroskopisinin PCA metodu ile kombinasyonunun kaçak veya yasal olmayan yollarla üretilen alkollü içeceklerdeki bileşenlerin kontrolünde tarama yöntemi olarak kullanılabileceği sonucuna varıldı.

Keywords

Raman Spektroskopisi, Alkollü içecek, Temel Bileşenler Analizi

Ethical Statement

Çalışma insan veya hayvan incelemelerini kapsamadığından dolayı etik kurul alınmamıştır.

Supporting Institution

Bu araştırma kamu, ticari veya kar amacı gütmeyen sektörlerdeki herhangi bir finansman kuruluşundan özel bir hibe almamıştır.

Thanks

Çalışmada, Boğma Rakı numunelerinin toplanmasında her türlü katkı sunan merhum meslektaşımız Dr. Öğr. Üyesi Berna HAMAMCI'ya (06.02.2023) teşekkür ederim.

Raman Spectroscopy and Principal Components Analysis in The Qualitative Detection of Ethanol in Homemade Alcoholic Beverages

Abstract

Excessive consumption of alcoholic and carbonated beverages containing ethanol can lead to impaired judgment, loss of consciousness, coma and even death. The aim of the study was to qualitatively analyze the ethanol in Boğma Rakı samples, an alcoholic beverage specific to Turkey, using Raman Spectroscopy and Principal Components Analysis (PCA) method. Boğma Rakı samples (22 pieces), which are known to have been produced under different conditions, were examined with Raman spectroscopy to determine specific bands of ethanol. OriginPro 2022 program was used to apply the PCA method to the data obtained. In the study, it was observed that the most prominent band belonging to ethanol was at 883 1/cm in the range of 100-1600 1/cm. Two PCs (Principal Components) values were obtained as a result of the PCA method used to classify the samples. The total PC1 value was 95.22% and the total PC2 value was 4.64%, and it was seen that the sum of these values constituted 99.86% of the total variance of the data set. It was determined that the obtained PC1 and PC2 values were related to the ethanol of the samples. It has been shown that examining Raman spectroscopy of alcoholic beverages and applying chemometric methods to the data obtained is effective in detecting ethanol in alcoholic beverages. The study concluded that the combination of Raman spectroscopy with the PCA method can be used as a screening method in the control of components in legally or illegally produced alcoholic beverages.

Keywords

Raman spectroscopy, Alcoholic beverages, Principial Component Analysis

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