BACTERICIDAL EFFECTS OF VARIOUS IRRIGATION SOLUTIONS AGAINST STAPHYLOCOCCUS AUREUS IN HUMAN ROOT CANAL

Year 2015, Volume: 49 Issue: 1, 19 - 26, 25.03.2015

Recai Zan , Gizem Kutlu İhsan Hubbezoglu , Zeynep Sumer , Tutku Tunc , Zuhal Mutlu

https://doi.org/10.17096/jiufd.70199

Abstract

Purpose: The aim of the present study was to evaluate and to compare the antibacterial effects of various irrigation solutions against Staphylococcus aureus (S. aureus) in human root canals.
Materials and Methods: 120 single-root mandibular premolar teeth were selected. The teeth were prepared and sterilized. S. aureus was incubated in the root canals and kept at 37°C for 24h. The infected root canals were divided into one positive (saline) and one negative (sodium hypochlorite) control, and four experimental groups [Ethylene-diaminetetra-aceticacid, Chlorhexidine Gluconate, Super-oxidized water(SPO), Aqueous ozone] (n=20). Flow rate of irrigation was applied with 5 mL/min flow rate for 3 min to ensure standardization among all study groups. Following the irrigation, paper points were placed in the root canals and then transferred in sterile eppendorf. Remaining bacteria were counted on blood agar plates and the data were analyzed using one-way ANOVA and Tukey’s test.
Results: Although there were statistically significant differences among SPO and other experimental groups (p<0.05), there was no statistically significant difference between SPO and NaOCl (p>0.05).
Conclusion: Super-oxidized water may be recommended as an alternative irrigation solution instead of NaOCl against S. aureus in root canals.

Keywords

Disinfection; irrigants; Staphylococcus aureus

Çeşitli İrrigasyon Solüsyonlarının İnsan Kök Kanalındaki Staphylococcus aureus’a Karşı Antibakteriyel Etkileri

Abstract

Amaç: Bu çalışmanın amacı, insan kök kanallarında Staphylococcus aureus (S. aureus)’a karşı çeşitli irrigasyon solüsyonlarının antibakteriyel etkilerini değerlendirmek ve karşılaştırmaktır.Gereç ve Yöntem: 120 tek kök mandibular premolar dişler seçildi. Dişler genişletildi ve sterilize edildi. S. aureus kök kanalı içinde inkübe edildi ve 24 saat boyunca 37°C’de tutuldu. Enfekte kök kanalları bir pozitif (serum fizyolojik) ve bir negatif (sodyum hipoklorit) kontrol ve dört deney grubuna (Etilen diamin tetra asetik asit, Chlorhexidine Gluconate, Super-oxidized water, Aqueous ozone) ayrıldı (n=20). İrrigasyon akış hızı, tüm çalışma grupları arasında standardizasyonu sağlamak amacıyla 5 mL/dakika akış hızıyla 3 dakika boyunca uygulandı. İrrigasyonu takiben kağıt koniler kök kanalı içine yerleştirildi ve daha sonra steril eppendorf tüplerine aktarıldı. Kalan bakteriler kanlı agar plakaları üzerinde sayıldı ve veriler tek yönlü ANOVA ve Tukey testi kullanılarak analiz edildi.Bulgular: Süper-okside su ile diğer deney grupları arasında istatistiksel olarak anlamlı fark olmasına rağmen (p<0,05), SPO ile NaOCl arasında istatistiksel olarak anlamlı bir fark bulunamamıştır (p>0,05). Sonuç: Süper-okside su, kök kanallarındaki S. aureus’a karşı NaOCl’ye alternatif bir irrigasyon solüsyonu olarak tavsiye edilebilir

Keywords

Dezenfeksiyon, irrigan, Staphylococcus aureus

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