Sıçanlarda Etanol ile İndüklenen Gastrik Ülser Modelinde Idebenon ve Krisinin Koruyucu Etkileri

Year 2025, Volume: 5 Issue: 1, 30 - 37, 17.03.2025

Arzu Gezer , Ali Sefa Mendil , Gürsel Bedir , Hilal Üstündağ

https://doi.org/10.62425/jlasp.1561168

Abstract

Bu çalışmada, sıçanlarda etanol ile indüklenen gastrik ülsere karşı idebenon ve krisinin koruyucu etkileri araştırılmıştır. Yirmi sekiz Sprague-Dawley erkek sıçan dört gruba ayrılmıştır: 1 ml serum fizyolojik alan kontrol grubu, gastrik ülser indüklemek için etanol (ET) (5 ml/kg) alan ET grubu ve iki tedavi grubu; biri ET'yi takiben idebenon (200 mg/kg), diğeri ET'yi takiben krisin (100 mg/kg) alan gruplar. Tedaviler oral gavaj yoluyla uygulanmıştır. Gastrik mukozal hasarı ve tedavilerin koruyucu mekanizmasını değerlendirmek için, somatostatin ve aquaporin-1 düzeylerine odaklanan histopatolojik incelemeler ve immünohistokimyasal analizler gerçekleştirilmiştir. Histopatolojik incelemelerde, ET ile muamele edilen grupta şiddetli dejeneratif ve nekrotik değişiklikler gözlenmiştir. Sonuçlar, tedavi gruplarında gastrik mukozal hasarın ET grubuna kıyasla önemli ölçüde azaldığını ve nekrotik lezyonların şiddetinde anlamlı bir azalma olduğunu göstermiştir. Kantitatif analiz, tedavi gruplarında somatostatin ve aquaporin-1 ekspresyonunda anlamlı bir artış olduğunu ortaya koymuştur (P < 0,05), bu da artmış gastrik mukozal savunmayı göstermektedir. İdebenon ve krisinin, ET ile indüklenen gastrik ülserlerde histopatolojik hasarı önemli ölçüde azalttığı ve somatostatin ile aquaporin-1 ekspresyonunu artırdığı bulunmuştur. Nekrotik lezyonlardaki azalma ve gastrik mukozal savunma mekanizmalarının güçlenmesi, idebenon ve krisinin gastrik ülserlerin önlenmesi ve tedavisi için terapötik potansiyel sunabileceğini düşündürmektedir.

Keywords

Etil alkol, gastrik ülser, idebenon, krisin.

THE PROTECTIVE EFFECTS OF IDEBENONE AND CHRYSIN ON ETHANOL-INDUCED GASTRIC ULCER IN RATS

Abstract

Objective: In this study, the protective effects of idebenone and chrysin against ethanol-induced gastric ulcers in rats were investigated.
Material and methods: Twenty-eight Sprague-Dawley male rats were categorized into four groups: a control group receiving 1 ml saline, an ethanol (ET) group receiving ET (5 ml/kg) to induce gastric ulcers, and two treatment groups, one receiving ET followed by idebenone (200 mg/kg) and the other receiving ET followed by chrysin (100 mg/kg). Treatments were administered orally via gavage. Histopathological examinations and immunohistochemical analyses focusing on somatostatin and aquaporin-1 levels were performed to evaluate gastric mucosal damage and the protective mechanism of the treatments.
Results: In histopathological examinations, severe degenerative and necrotic changes were observed in the ET treated group. The results showed that gastric mucosal damage was significantly reduced in the treatment groups compared to the ET group and there was a significant reduction in the severity of necrotic lesions. Quantitative analysis revealed a significant increase in somatostatin and aquaporin-1 expression in the treatment groups (P < 0.05), indicating increased gastric mucosal defence.
Conclusion: Idebenone and chrysin were found to significantly mitigate histopathological damage and increase the expression of somatostatin and aquaporin-1 in ET-induced gastric ulcers. The reduction in necrotic lesions and enhancement of gastric mucosal defense mechanisms suggest that idebenone and chrysin may offer therapeutic potential for the prevention and treatment of gastric ulcers.

Keywords

Chrysin, ethyl alcohol, gastric ulcer, idebenone

Ethical Statement

Ethical approval was obtained from the Ataturk University Animal Experiments Local Ethics Committee under the decision dated February 26, 2024, and desicion number 2024/43.

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