Çizgili Yılan Balığının Plotosus lineatus (Thunberg, 1787) İskenderun Körfezi’nden (Doğu Akdeniz, Türkiye) Genetik Doğrulaması

Year 2024, Volume: 7 Issue: 1, 24 - 29, 10.07.2024

Cemal Turan , Ayşegül Ergenler , Funda Turan , Servet Ahmet Doğdu

https://doi.org/10.46384/jmsf.1449570

Abstract

Çizgili yılan balığı Plotosus lineatus'un Akdeniz'deki kayıtları morfolojik karakterlere dayanılarak belirlenmiştir. Bununla birlikte, morfolojik taksonomik belirlemeyi doğrulamak ve yanlış tanımlamayı önlemek için moleküler ve genetik analizlere ihtiyaç duyulmaktadır. Bu çalışmada, Akdeniz'deki Plotosus lineatus'un genetik doğrulaması için mitokondriyal sitokrom c oksidaz alt ünite I (COI) gen bölgesi kullanılmıştır. Elde edilen P. lineatus dizilerinin Kızıldeniz ve Akdeniz kayıtları ile %100 örtüştüğü tespit edilmiştir. Mevcut moleküler genetik çalışma ile P. lineatus'un Türk deniz sularındaki varlığını doğrulamaktadır. Ayrıca, bu çalışma P. lineatus'un Türkiye kıyılarında ve Akdeniz'de ilk genetik doğrulamasıdır.

Keywords

Plotosus lineatus, Genetik doğrulama, Yılan balığı, İstilacı tür, mtDNA COI

Genetic Confirmation of the Striped Eel Catfish Plotosus lineatus (Thunberg, 1787) from Iskenderun Bay (Eastern Mediterranean, Türkiye)

Abstract

The records of the striper eel catfish Plotosus lineatus have been assigned based on morphological characters in the Mediterranean Sea. However, molecular and genetic analyses are needed to confirm the morphological taxonomic determination and avoid misidentification. In this study, the mitochondrial cytochrome c oxidase subunit I (COI) gene region was used for genetic confirmation of Plotosus lineatus in the Mediterranean Sea. The obtained P. lineatus sequences were found to have a 100% identity match with the Red Sea and Mediterranean records. The present molecular genetic study confirms the presence of P. lineatus in Turkish marine waters. Also, this study is the first genetic confirmation of P. lineatus on the coast of Türkiye and in the Mediterranean Sea.

Keywords

Plotosus lineatus, Genetic confirmation, Eel catfish, Invasive species, mtDNA COI

Ethical Statement

Ethics committee approval is not necessary for this study.

References

• Ali, M., Saad, A., & Soliman, A. (2015). Expansion confirmation of the Indo-Pacific catfish, Plotosus lineatus (Thunberg, 1787),(Siluriformes: Plotosidae) into Syrian marine waters. American Journal of Biology and Life Sciences, 3(1), 7-11.
• Antil, S., Abraham, J. S., Sripoorna, S., Maurya, S., Dagar, J., Makhija, S., ... & Toteja, R. (2023). DNA barcoding, an effective tool for species identification: a review. Molecular Biology Reports, 50(1), 761-775. doi: 10.1007/s11033-022-08015-7
• Azzurro, E., & D’Amen, M. (2022). Climate change paves the way for a new inter‐ocean fish interchange. Frontiers in Ecology and the Environment, 20(10), 558-563. doi: 10.1002/fee.2459
• Azzurro, E., Smeraldo, S., & D'Amen, M. (2022). Spatio‐temporal dynamics of exotic fish species in the Mediterranean Sea: Over a century of invasion reconstructed. Global Change Biology, 28(21), 6268-6279. doi: 10.1111/gcb.16362
• Barathkumar, T. R., & Thangaraj, M. (2020). Phylogenetic observation in Ariidae, Bagridae and Plotosidae catfishes by COI gene sequence analysis. Notulae Scientia Biologicae, 12(4), 781-793. doi: 10.15835/nsb12410822
• Bariche, M., Torres, M., Smith, C., Sayar, N., Azzurro, E., Baker, R., & Bernardi, G. (2015). Red Sea fishes in the Mediterranean Sea: a preliminary investigation of a biological invasion using DNA barcoding. Journal of Biogeography, 42(12), 2363-2373. doi: 10.1111/jbi.12595
• Chaikin, S., De-Beer, G., Yitzhak, N., Stern, N., & Belmaker, J. (2023). The invasive silver-cheeked toadfish (Lagocephalus sceleratus) predominantly impacts the behavior of other non-indigenous species in the Eastern Mediterranean. Biological Invasions, 25(4), 983-990. doi: 10.1007/s10530-022-02972-7
• Dahruddin, H., Hutama, A., Busson, F., Sauri, S., Hanner, R., Keith, P., ... & Hubert, N. (2017). Revisiting the ichthyodiversity of Java and Bali through DNA barcodes: taxonomic coverage, identification accuracy, cryptic diversity and identification of exotic species. Molecular Ecology Resources, 17(2), 288-299. doi: 10.1111/1755-0998.12528
• Deidun, A., Maggio, T., Castriota, L., Falautano, M., & Franzitta, G. (2020). Genetic confirmation of the first Mediterranean record of Holacanthus africanus Cadenat, 1951. Journal of the Black Sea / Mediterranean Environment, 26 (1), 112-118.
• Dhar, B., & Ghosh, S. K. (2015). Genetic assessment of ornamental fish species from North East India. Gene, 555(2), 382-392. 10.1016/j.gene.2014.11.037
• Doğdu, S. A., & Turan, C. (2021). Authentication and Traceability of Pufferfish‎ Species Using DNA Sequencing. Pakistan Journal of Marine Sciences, 30(1), 1-11.
• Doğdu, S. A., & Turan, C. (2024). Biological and growth parameters of Plotosus lineatus in the Mediterranean Sea. PeerJ, 12, e16945. doi: 10.7717/peerj.16945
• Doğdu, S. A., Uyan, A., Uygur, N., Gürlek, M., Ergüden, D., & Turan, C. (2016). First record of the Indo-Pacific striped eel catfish, Plotosus lineatus (Thunberg, 1787) from Turkish marine waters. Natural and Engineering Sciences, 1(2), 25-32. doi: 10.28978/nesciences.286245
• Doğdu, S. A., Sakalli, U., Gürlek, M., & Turan, C. (2019). The first record of the Lesser amberjack Seriola fasciata (Bloch, 1793) in the Çevlik coast of Turkey, Eastern Mediterranean Sea. Biharean Biologist, 13(1), 55-57.
• Felsenstein, J. (1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39(4), 783-791. doi: 10.1111/j.1558-5646.1985.tb00420.x
• Fricke, R., Eschmeyer, W.N., Van der Laan, R., (2023). Eschmeyer's Catalog of Fishes: Genera, Species, References. (http://researcharchive.calacademy.org/research/ichthyology/ catalog/fishcatmain.asp). Electronic version accessed 20/12/2023.
• Froese, R. and Pauly, D. (2023). FishBase. World Wide Web electronic publication. www.fishbase.org, version (10/2023).
• Galil, B. S., Marchini, A., & Occhipinti-Ambrogi, A. (2018). Mare Nostrum, mare quod invaditur the history of bioinvasions in the Mediterranean Sea. Histories of Bioinvasions in the Mediterranean, 21-49.
• Golani, D. (2002). The Indo-Pacific striped eel catfish, Plotosus lineatus (Thunberg, 1787),(Osteichtyes: Siluriformes), a new record from the Mediterranean. Scientia Marina, 66(3), 321-323. doi: 10.3989/scimar.2002.66n3321
• Golani, D., Azzurro, E., Dulčić, J., Massutí, E., & Orsi Relini, L. (2021). Atlas of exotic fishes in the Mediterranean Sea (2nd ed.) (365 p. CIESM Publishers). Monaco: CIESM Publishers.
• Goren, M., Stern, N., & Diamant, A. (2020). New records of Plotosus japonicus in the Red Sea and genetic indications for its presence throughout the Indo-Pacific (Osteichthyes: Plotosidae). Zoology in the Middle East, 66(2), 124-131. doi: 10.1080/09397140.2020.1729560
• Hall, T., Biosciences, I., & Carlsbad, C. J. G. B. B. (2011). BioEdit: an important software for molecular biology. GERF Bull Biosci, 2(1), 60-61.
• Hebert, P. D., Cywinska, A., Ball, S. L., & DeWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences, 270(1512), 313-321. doi: 10.1098/rspb.2002.2218
• Isaacs, R. B., & Hellberg, R. S. (2020). Authentication of red snapper (Lutjanus campechanus) fillets using a combination of real-time PCR and DNA barcoding. Food Control, 118, 107375. doi: 10.1016/j.foodcont.2020.107375
• Karan, S., Uyan, A., Doğdu, S. A., Gürlek, M., Ergüden, D., & Turan, C. (2019). Genetic confirmation of Red cornetfish, Fistularia petimba (Syngnathiformes: Fistularidae) occurrence in Turkish marine waters. FishTaxa-Journal of Fish Taxonomy, 4(3).
• Katsanevakis, S., Wallentinus, I., Zenetos, A., Leppäkoski, E., Çinar, M. E., Oztürk, B., ... & Cardoso, A. C. (2014). Impacts of invasive alien marine species on ecosystem services and biodiversity: a pan-European review. Aquatic Invasions 9: 391–423. doi: 10.3391/ai.2014.9.4.01
• Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547. doi: 10.1093/molbev/msy096
• Kundu, S., Pakrashi, A., Laskar, B. A., Rahaman, I., Tyagi, K., Kumar, V., & Chandra, K. (2019). DNA barcoding reveals distinct population of Plotosus canius (Siluriformes: Plotosidae) in Sundarbans waters. Mitochondrial DNA Part B, 4(1), 1167-1171. doi: 10.1080/23802359.2019.1591172
• Landi, M., Dimech, M., Arculeo, M., Biondo, G., Martins, R., Carneiro, M., ... & Costa, F. O. (2014). DNA barcoding for species assignment: the case of Mediterranean marine fishes. PLoS one, 9(9), e106135. doi: 10.1371/journal.pone.0106135
• Langeneck, J., Bakiu, R., Chalari, N., Chatzigeorgiou, G., Crocetta, F., Doğdu, S. A., ... & Zenetos, A. (2023). New records of introduced species in the Mediterranean Sea (November 2023). Mediterranean Marine Science, 24(3), 610-632. doi: 10.12681/mms.35840
• Liu, Y. H., & Zhang, M. H. (2016). The complete mitochondrial genome sequence of Plotosus japonicus (Siluriformes: Plotosidae) and phylogenetic studies of Siluriformes. Mitochondrial DNA Part A, 27(4), 2791-2792. doi: 10.3109/19401736.2015.1053069
• Mutlu, E., Meo, I. D., Miglietta, C., & Deval, M. C. (2023). Ecological Indicative Stressors of Native vs. Non-Native Fish in an Ultra-Oligotrophic Region of the Mediterranean Sea. Sustainability, 15(3), 2726. doi: 10.3390/su15032726
• Nakatani, M., Miya, M., Mabuchi, K., Saitoh, K., & Nishida, M. (2011). Evolutionary history of Otophysi (Teleostei), a major clade of the modern freshwater fishes: Pangaean origin and Mesozoic radiation. BMC Evolutionary Biology, 11, 1-25. doi: 10.1186/1471-2148-11-177
• Samani, N. K., Esa, Y., Amin, S. N., & Ikhsan, N. F. M. (2016). Phylogenetics and population genetics of Plotosus canius (Siluriformes: Plotosidae) from Malaysian coastal waters. PeerJ, 4, e1930. doi: 10.7717/peerj.1930
• Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Molecular cloning: a laboratory manual (No. Ed. 2). Cold spring harbor laboratory press.
• Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 74(12), 5463-5467. doi: 10.1073/pnas.74.12.5463
• Shirak, A., Dor, L., Seroussi, E., Ron, M., Hulata, G., & Golani, D. (2016). DNA barcoding of fish species from the Mediterranean coast of Israel. Mediterranean Marine Science, 17(2), 459-466. doi: 10.12681/mms.1384
• Temraz, T., & Ben Souissi, J. B. (2013). First record of striped eel catfish Plotosus lineatus (Thunberg, 1787) from Egyptian waters of the Mediterranean. Rapport de la Commission International pour l’Exploration Scientifique de la MerMediterrane, 40, 604.
• Tiralongo, F., Crocetta, F., Riginella, E., Lillo, A. O., Tondo, E., Macali, A., & Azzurro, E. (2020). Snapshot of rare, exotic and overlooked fish species in the Italian seas: A citizen science survey. Journal of Sea Research, 164, 101930. doi: 10.1016/j.seares.2020.101930
• Turan, C., & Doğdu, S. A. (2022). Preliminary Assessment of Invasive Lionfish Pterois miles Using Underwater Visual Census Method in the Northeastern Mediterranean. Croatian Journal of Fisheries: Ribarstvo, 80(1), 38-46. doi: 10.2478cjf-2022-0005
• Turan, C. and Doğdu, S.A. (2023). Mapping Abundance and Distribution of Indo-Pacific Striped Eel Catfish Plotosus lineatus in The Northeastern Mediterranean Sea. Ecological Life Sciences, 18(1):1-7. doi: 10.12739/NWSA.2023.18.1.5A0187
• Turan, C., Ergüden, D., Gürlek, M. (2016). Climate Change and Biodiversity Effects in Turkish Seas. Natural and Engineering Sciences, 1 (2), 15-24. doi: 10.28978/nesciences.286240
• Turan, C., Gürlek, M., Ergüden, D., Uyan, A., Karan, S., & Doğdu, S. A. (2017). Assessing DNA barcodes for identification of pufferfish species (Tetraodontidae) in Turkish marine waters. Natural and Engineering Sciences, 2(3), 55-66. doi: 10.28978/nesciences.369538
• Turan, C., Gürlek, M., Başusta, N., Uyan, A., Doğdu, S. A., & Karan, S. (2018). A checklist of the non-indigenous fishes in Turkish marine waters. Natural and Engineering Sciences, 3(3), 333-358. doi: 10.28978/nesciences.468995
• Turan C., Uyan A., Gürlek M., Doğdu S.A. 2020. DNA barcodes for identifications of two lionfish species Pterois miles(Bennett, 1828) and P. volitans (Linnaeus, 1758) in the Mediterranean. FishTaxa 16: 29-36
• Turan, C., Ayas, D., Doğdu, S. A., & Ergenler, A. (2022). Extension of the striped eel catfish Plotosus lineatus (Thunberg, 1787) from the eastern Mediterranean coast to the Mersin Bay on the western Mediterranean coast of Turkey. Natural and Engineering Sciences, 7(3), 240-247. doi: 10.28978/nesciences.1183740
• Vella, N., Vella, A., & Agius Darmanin, S. (2016). Morphological and genetic analyses of the first record of the Niger Hind, Cephalopholis nigri (Perciformes: Serranidae), in the Mediterranean Sea and of the African Hind, Cephalopholis taeniops, in Malta. Marine Biodiversity Records, 9(1), 1-5. doi: 10.1186/s41200-016-0101-y
• Ward, R. D., Zemlak, T. S., Innes, B. H., Last, P. R., & Hebert, P. D. (2005). DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462), 1847-1857. doi: 10.1098/rstb.2005.1716
• Watkins, H. V., Yan, H. F., Dunic, J. C., & Côté, I. M. (2021). Research biases create overrepresented “poster children” of marine invasion ecology. Conservation Letters, 14(3), e12802. doi: 10.1111/conl.12802
• Yağlioğlu, D., Doğdu, S. A., & Turan, C. (2023). First Morphological and Genetic Record and Confirmation of Korean Rockfish Sebastes schlegelii Hilgendorf, 1880 in the Black Sea Coast of Türkiye. Natural and Engineering Sciences, 8(3), 140-150. doi: 10.28978/nesciences.1363941