Effectiveness of Propolis on Experimental Colitis Model In Rats

Year 2024, Volume: 7 Issue: 3, 158 - 164, 30.09.2024

Levent Bolat , Cem Kaan Parsak , Uğur Topal , Burak Yavuz , Emine Kılıç Bağır , Yusuf Döğüş , Ozlem Gorur , Gülşah Seydaoğlu , İsmail Cem Eray , Gürhan Sakman

https://doi.org/10.36516/jocass.1517421

Abstract

Aim: This study investigated the therapeutic potential of propolis in an experimental colitis model induced by acetic acid in female Wistar albino rats.
Materials and Methods: Thirty rats were divided into five groups: a control group and four experimental groups. Colitis was induced in the second, third, and fifth groups by rectal administration of 1 ml of 4% acetic acid. The third group received rectal propolis solution (50 mg/ml), while the fourth group was given only rectal propolis solution. The fifth group received 1 ml of olive oil rectally after the onset of colitis. Stool consistency and weight loss were monitored, and colon tissue samples were collected for microscopic and macroscopic evaluation. The levels of MDA, MPO, and caspase-3 in tissue, as well as TNF-α and IL-10 levels in blood samples, were examined.
Results: The group administered propolis showed a significant decrease in microscopic and macroscopic scores compared to the other experimental groups. The levels of MDA, MPO, and caspase-3 in the tissue, as well as TNF-α and IL-10 levels in blood samples, were significantly decreased in the propolis group compared to the other experimental groups. Weight loss and stool consistency also showed improvement in the propolis group compared to the other experimental groups.
Discussion: Propolis may have therapeutic effects in experimental colitis induced by acetic acid. The decrease in oxidative damage and inflammation seen in the propolis group indicates that it may be a useful therapeutic agent for colitis treatment.

Keywords

Acetic acids, Colitis, Ulcerative, Rats, Wistar, Bee products, Olive oil.

Ethical Statement

This study was conducted in accordance with ethical guidelines and received approval from the ethics committee of Çukurova University Animal Experiments Local Ethics Committee, dated 20.01.2022 with the decision number 1.

Supporting Institution

Cukurova University Research Fund

Project Number

TTU-2022-14631

References

• 1.Molodecky NA, Soon IS, Rabi DM, et al. Increasing Incidence and Prevalence of the Inflammatory Bowel Diseases With Time, Based on Systematic Review. Gastroenterology 2012; 142:46-54.e42. https://doi.org/10.1053/j.gastro.2011.10.001
• 2.Flynn S, Eisenstein S. Inflammatory Bowel Disease Presentation and Diagnosis. Surgical Clinics of North America 2019; 99:1051-62. https://doi.org/10.1016/j.suc.2019.08.001
• 3.Kelsen JR, Russo P, Sullivan KE. Early-Onset Inflammatory Bowel Disease. Immunol Allergy Clin North Am 2019; 39:63-79. https://doi.org/10.1016/j.iac.2018.08.008
• 4.Ananthakrishnan AN. Epidemiology and risk factors for IBD. Nature Reviews Gastroenterology & Hepatology 2015; 12:205-17. https://doi.org/10.1038/nrgastro.2015.34
• 5.Burisch J, Munkholm P. The epidemiology of inflammatory bowel disease. Scandinavian Journal of Gastroenterology 2015; 50:942-51. https://doi.org/10.3109/00365521.2015.1014407
• 6.Mowat C, Cole A, Windsor A, et al. Guidelines for the management of inflammatory bowel disease in adults. Gut 2011; 60:571-607. https://doi.org/10.1136/gut.2010.224154
• 7.Rashidian A, Roohi P, Mehrzadi S, Ghannadi AR, Minaiyan M. Protective Effect of Ocimum basilicum Essential Oil Against Acetic Acid-Induced Colitis in Rats. Journal of Evidence-Based Complementary & Alternative Medicine 2016; 21:NP36-NP42. https://doi.org/10.1177/2156587215616550
• 8.Wang G, Xu B, Shi F, et al. Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response. Oxid Med Cell Longev 2019; 2019:7850324-. https://doi.org/10.1155/2019/7850324
• 9.Toreti VC, Sato HH, Pastore GM, Park YK. Recent progress of propolis for its biological and chemical compositions and its botanical origin. Evid Based Complement Alternat Med 2013; 2013:697390-. https://doi.org/10.1155/2013/697390
• 10.Bankova V, Dyulgerov A, Popov S, Marekov N. A GC/MS Study of the Propolis Phenolic Constituents. Zeitschrift für Naturforschung C 1987; 42:147-51. https://doi.org/10.1515/znc-1987-1-224
• 11.Krell R, Food, Nations AOotU. Value-added Products from Beekeeping: Food and Agriculture Organization of the United Nations, 1996.
• 12.Oruç N, Kutluana U, Sezak M, et al. Asetik asitle oluşturulan deneysel kolit modelinde leflunomid'in etkinliğinin araştırılması. The Turkish Journal of Academic Gastroenterology 2008; 7:67-72.
• 13.Ruiz-Hurtado PA, Garduño-Siciliano L, Domínguez-Verano P, et al. Propolis and Its Gastroprotective Effects on NSAID-Induced Gastric Ulcer Disease: A Systematic Review. Nutrients 2021; 13:3169. https://doi.org/10.3390/nu13093169
• 14.Krocko M, Čanigová M, Bezekova J, Lavová M, Haščík P, Ducková V. Effect of Nutrition with Propolis and Bee Pollen Supplements on Bacteria Colonization Pattern in Gastrointestinal Tract of Broiler Chickens. Animal Science and Biotechnologies 2012; 45:63-7.
• 15.Wang K, Jin X, Li Q, et al. Propolis from Different Geographic Origins Decreases Intestinal Inflammation and <i>Bacteroides</i> spp. Populations in a Model of DSS-Induced Colitis. Molecular Nutrition &amp; Food Research 2018; 62:1800080. https://doi.org/10.1002/mnfr.201800080
• 16.Khan MN, Lane ME, McCarron PA, Tambuwala MM. Caffeic acid phenethyl ester is protective in experimental ulcerative colitis via reduction in levels of pro-inflammatory mediators and enhancement of epithelial barrier function. Inflammopharmacology 2018; 26:561-9. https://doi.org/10.1007/s10787-017-0364-x
• 17.Gonçalves CCM, Hernandes L, Bersani-Amado CA, Franco SL, Silva JFdS, Natali MRM. Use of propolis hydroalcoholic extract to treat colitis experimentally induced in rats by 2,4,6-trinitrobenzenesulfonic Acid. Evid Based Complement Alternat Med 2013; 2013:853976-. https://doi.org/10.1155/2013/853976
• 18.Aslan A, Temiz M, Atik E, et al. Effectiveness of mesalamine and propolis in experimental colitis. Advances in Therapy 2007; 24:1085-97. https://doi.org/10.1007/BF02877715
• 19.Liu X, Wang JM. Iridoid glycosides fraction of Folium syringae leaves modulates NF-κB signal pathway and intestinal epithelial cells apoptosis in experimental colitis. PLoS One 2011; 6:e24740-e. https://doi.org/10.1371/journal.pone.0024740
• 20.Crespo I, San-Miguel B, Prause C, et al. Glutamine treatment attenuates endoplasmic reticulum stress and apoptosis in TNBS-induced colitis. PLoS One 2012; 7:e50407-e. https://doi.org/10.1371/journal.pone.0050407
• 21.Murad H, Ahmed O, Alqurashi T, Hussien M. Olmesartan medoxomil self-microemulsifying drug delivery system reverses apoptosis and improves cell adhesion in trinitrobenzene sulfonic acid-induced colitis in rats. Drug Deliv 2022; 29:2017-28. https://doi.org/10.1080/10717544.2022.2086939