Effect of Hydro-Priming on Seed Germination and Early Seedling Growth in Three Cucurbit Rootstock Cultivars under Salt and Osmotic Stresses

Year 2021, Volume: 2 Issue: 1, 1 - 5, 30.06.2021

Sıtkı Ermiş , Güleda Öktem , Zeynep Gökdaş , İbrahim Demir

Abstract

In this study, the effects of hydro-priming was assessed to enhance germination and seedling growth of three cucurbit rootstock cultivar (Nun 9075, Shintosa and Jumbo) seeds. Seeds were soaked at 25°C over 24 hours in the dark, hydro-primed and control seeds were then germinated at 100 mM NaCl, salt and -0.6 MPa Polyethylene glycol (PEG-6000), osmotic stress conditions. Germination percentages, mean germination time, root length, root fresh and dry weight, seedling fresh and dry weights were measured after 7 days-old seedlings. Results showed that hydro-priming increased germination percentages and seedling growth parameters under both stress conditions in all three cultivars. The findings suggested that hydro-priming can be an effective method to increase germination potential and seedling growth in rootstock cultivar seeds.

Keywords

Seed treatments, rootstock seed, cucurbit, hydro-priming, stress conditions

Hidropriming Uygulamasının Farklı Anaç Kabak Tohumlarında Tuz ve Ozmotik Stres Altında Çimlenme ve Erken Fide Gelişimi Üzerine Etkileri

Abstract

Bu araştırmada, farklı kabak anaç çeşitlerine ait tohumlarda (Nun 9075, Shintosa ve Jumbo) hidropriming uygulamalarının tohum çimlenmesi ve fide gelişimi üzerine etkileri araştırılmıştır. Tohumlar karanlık bir ortamda 25°C sıcaklıkta 24 saat boyunca suda bekletilmiştir. Hidropriming uygulanan tohumlar ve kontrol grupları daha sonra 100 mM NaCl, tuz ve -0.6 MPa Polietilen glikol (PEG-6000) ile sağlanan osmotik stres koşullarında çimlendirilmiştir. 7 gün sonra çimlenme yüzdeleri, ortalama çimlenme hızı, kök uzunluğu, yaş ve kuru sürgün ve kök ağırlıkları ölçülmüştür. Hidropriming uygulaması sonucunda, anaç kabak çeşitlerine ait tohumların çimlenme yüzdelerinin ve fide gelişim parametrelerinin her iki stres koşulunda arttığı görülmüştür. Elde edilen bulgular hidropriming uygulamasının, kabak anacı çeşitlerinin tohumlarında çimlenme potansiyelini ve fide gelişimini arttırmada etkili bir yöntem olabileceğini göstermiştir.

Keywords

Tohum uygulamaları, anaç tohumlar, kabakgiller, hydro-priming, stres koşulları

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