Assessment of biomarkers indicating activation of the complement system in pregnant women with fetal growth restriction

Year 2024, Volume: 5 Issue: 2, 129 - 134, 30.04.2024

Fırat Ersan , Işıl Turan Bakırcı , Gülsen Şener , Nihal Çallıoğlu , Selçuk Atalay , Güray Tuna

https://doi.org/10.47582/jompac.1466260

Abstract

Aims: To compare serum levels of sC5b-9, C3, C4, C1-INH, and CH50, which are indicators of complement system activation and regulatory processes, in pregnant women with and without fetal growth restriction (FGR).
Methods: This study enrolled eighty-six women with gestational age between 24 and 36 weeks. Maternal blood samples were obtained from 43 patients diagnosed with FGR and 43 from healthy pregnancies. Serum complement levels were measured using commercially available ELISA kits according to the manufacturer’s instructions (SunRed, China).
Results: When the levels of complement activation biomarkers of pregnancies with FGR were compared with those of healthy pregnancies, the C1est level was significantly higher, C4 and CH50 levels were slightly lower, and Sc5b9 and C3 levels were similar.
Conclusion: While the exact role of complement activation in FGR remains fully elucidated, the elevated levels of C1-INH in women with FGR suggest a compensatory mechanism to mitigate thrombus formation and inflammation. This adaptive response may be a potential therapeutic target for improving placental function and pregnancy outcomes.

Keywords

Complement system, fetal growth restriction, pregnancy, placenta

Etal büyüme kısıtlılığı olan hamile kadınlarda kompleman sisteminin aktivasyonunu gösteren biyobelirteçlerin değerlendirilmesi

Abstract

Amaç Fetal büyüme kısıtlılığı (FGR) olan ve olmayan gebelerde kompleman sistemi aktivasyonu ve düzenleyici süreçlerin göstergeleri olan sC5b-9, C3, C4, C1-INH ve CH50 serum düzeylerini karşılaştırmak.

Çalışma Tasarımı. Bu çalışmaya gebelik yaşı 24 ila 36 hafta arasında olan seksen altı kadın dahil edilmiştir. Maternal kan örnekleri, FGR tanısı konmuş 43 hastadan ve 43 sağlıklı gebelikten elde edilmiştir. Serum kompleman düzeyleri ticari olarak temin edilebilen ELISA kitleri kullanılarak üreticinin talimatlarına göre ölçülmüştür (SunRed, Çin).

Sonuçlar. FGR'li gebelerin kompleman aktivasyon biyobelirteçlerinin seviyeleri sağlıklı gebelerinkiyle karşılaştırıldığında, C1est seviyesi anlamlı derecede yüksek, C4 ve CH50 seviyeleri hafif düşük ve Sc5b9 ve C3 seviyeleri benzerdi.

Sonuçlar. Sonuç olarak, FGR'de kompleman aktivasyonunun rolü tam olarak aydınlatılamamış olsa da, FGR'li kadınlarda yüksek C1-INH seviyeleri trombüs oluşumunu ve enflamasyonu azaltmak için telafi edici bir mekanizma olduğunu düşündürmektedir. Bu adaptif yanıt, plasental fonksiyonu ve gebelik sonuçlarını iyileştirmek için potansiyel bir terapötik hedef olabilir.

Keywords

Kompleman sistemi, Fetal büyüme kısıtlılığı, Gebelik, Plasenta

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