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Lilium candidum L.’da In vitro Mikroçoğaltım ve Alkaloidler

Year 2016, Volume: 13 Issue: 3, 0 - 0, 19.09.2016

Sheida Daneshvar Royandazagh , Elif Ceren Pehlivan

Abstract

Bu çalışmada in vitro koşullarda mikroçoğaltım yöntemleriyle üretilen soğanlarda gelişimin gözlendiği dönemden itibaren 3 farklı dönemde (1., 2. ve 3. ay sonunda) soğancıklardan örnek alımı yapılmıştır. Örnek alınan soğancıkların ekstraksiyonu yapıldıktan sonra alkaloid (azotlu bileşik) profili GC-MS ile tanımlanmıştır ve in vivo’da yetişen soğanlarla karşılaştırarak mikroçoğaltım çalışmalarında in vivo koşullara en yakın örnek alım dönemi belirlenmiştir. Çalışmada L. candidum’un soğan pul yaprakları eksplant olarak kullanılmış ve rejenerasyon sonucu 3 farklı besin ortamından ve 3 farklı dönemde alınan soğancıklarda GC-MS analizi yapılmıştır.  Yapılan GC-MS analizi sonucunda toplam alkaloid yüzdesi kontrole (%13.11) en yakın örnek 2. besin ortamının (0.2 mg/L NAA + 0.4 mg/L TDZ) 3. örnek alımından (%7.42) elde edilmiştir. Belirlenen en yüksek yüzde alana sahip alkaloid (1H-Pyrrole, 2,5-dihydro-1-nitroso-) ise yine 2. besin ortamının 3. örnek alımında belirlenmiştir.

References

  • Anonim. Dünya Gıda Dergisi. 2011. (http://www.dunyagida.com.tr/haber.php?nid=3356).
  • Berkov, S., Pavlov, A., Ilieva, M., Burrus, M., Popov, S. and Stanilova, M. 2005. CGC-MS of alkaloids in Leucojum aestivum plants and their in vitro cultures. Phytochem. Anal. 16: 98-103.
  • Bogdanova, Y., Stoeva, T., Yanev, S., Pandova, S., Molle, E., Burrus, M. and Stanilova, M. 2009. Influenceof plant origin on propagation capacity and alkaloid biosynthesis during long- term in vitro cultivation of Leucojum aestivum L. In Vitro Cell. Dev.Biol.-Plant. 45: 458-465.
  • Bogdanova, Y., Pandova, S., Yanev, S. and Stanilova, M. 2014. Biosynthesis of lycorine by in vitro cultures of Pancratium maritimum L. (Amaryllidaceae). Biotechnol. & Biotechnol. Eq. 23(1): 919-922.
  • Cheng, H.D., Jie, G.X., Gen, X.P. and Yong, P. 2013. Phytochemical and biological research of Fritillaria medicinal resources. Chinese Journal of Natural Medicines. 11(4): 330-344.
  • Cupakova, M., Rauova, D., Vitkova, Z., Herdova, P., Grancai, D. and Haladova, M. 2012. Determination of kaempferol in extracts from Lilium Candidum L. Liliaceae by means of liquid chromatography. Acta Fac. Pharm. Uni. Comen. LIX: 14-21.
  • Daneshvar-Royandazagh, S., Pehlivan, E.C., Teykin, E.E. ve Çiftçi, H.S. 2014. Lilium candidum L.’da In Vitro Mikroçoğaltım ile Kozmetik Sanayisine Ham Madde Temini. Turkish Journal of Agricultural and Natural Sciences. Special Issue (2): 1911-1916.
  • Eisenreichova, E., Haladova, M., Buckova, A., Ubik, K. and Uhrin, D. 1991. Derivatives of pyrroline in Lilium candidum L. Chem. Papers. 45(5): 709-711.
  • Eisenreichova, E., Haladova, M., Buckova, A., Tomko, J., Uhrin, D. and Ubik, K. 1992. A pyrroline pyrrolidine alkaloid from Lilium candidum bulbs. Phtochemistry. 31(3): 1084-1085.
  • Eisenreichová, E., Haladová, M., Mučaji, P. and Grančai, D. 2004. The study of constituents of Lilium candidum L. Acta Fac Pharm. Univ Comen. 5: 27-37.
  • Erdoğan, I., Şener, B. and Rahman, A. 2001. Etioline, a steroidal alkaloid from Lilium candidum L. Biochemical Systematics and Ecology. 29: 535-536.
  • Georgiev, V., Ivanov, I., Berkov, S. and Pavlov, A. 2011. Alkaloids biosynthesis by Pancratium maritimum L. shoots in liquid culture. Acta Physiol Plant. 33: 927-933.
  • Georgiev, V., Ivanov, I., Berkov, S., Ilieva, M., Georgiev, M., Gocheva, T. and Pavlov, A. 2012. Galanthamine production by Leucojum aestivum L. shoot culture in a modified bubble column bioreactor with internal sections. Eng. Life Sci. 12(5): 534-543.
  • Haladová, M., Eisenreichová, E., Bůčková, A., Tomko, J. and Uhrín, D. 1988. New nitrogen- containing compounds in Lilium candidum L. Collect. Czechoslov. Chem. Commun. 53: 157-160.
  • Haladova, M., Mucaji, P., Budesinsky, M., Vokac, K., Cvacka, J., Grancai, D. and Eisenreichova, E. 2011. Spirostanol saponins from the bulbs of Lilium candidum. Chemistry of Natural Compounds. 46(6): 1004-1005.
  • Ivanov, I., Georgiev, V., Georgiev, M., Ilieva, M. and Pavlov, A. 2011. Galanthamine and related alkaloids production by Leucojum aestivum L. shoot culture using a temporary immersion technology. Appl Biochem Biotechnol. 163(2): 268- 277.
  • Khadem, S. and Marles, R.J. 2012. Chromone and Flavonoid Alkaloids: Occurrence and Bioactivity. Molecules. 17(1): 191-206.
  • Khawar, K.M., Çöçü, S., Parmaksız, I., Sarıhan, E.O. and Özcan, S. 2005. Mass proliferation of Madonna Lily (Lilium candidum L.) under ın vitro conditions. Pakistan Journal of Botany. 37(2): 243-248.
  • Mammadov, R. 2014. Tohumlu Bitkilerde Sekonder Metabolitler. Nobel Akademik Yayıncılık. Ankara, Türkiye, 412 s.--
  • Mimaki, Y., Satou, T. Kuroda, M., Sashida, Y. and Hatakeyama, Y. 1999. Steroidal saponins from the bulbs of Lilium candidum. Phytochemistry. 51(4): 567-573.
  • Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant. 15(3): 473-497.
  • Ondra, P., Valka, I., Vicar, J., Sütlüpınar, N. and Simanek, V. 1995. Chromatographic determination of constituents of the genus Colchicum (Li!iaceae). Journal of Chromatography A. 704: 351-356.
  • Özuslu, E. and İskender, E. 2009. Geophytes of Sof Mountain (Gaziantep/Turkey). Biological Diversity and Conservation. 2(2): 78-84.
  • Pavlov, A., Berkov, S., Courot, E., Gocheva, T, Tuneva, D, Pandova, B, Georgiev, M, Georgiev, V, Yanev, S, Burrus, M. and Ilieva, M. 2007. Galanthamine production by Leucojum aestivum in vitro systems. Process Biochemistry. 42: 734-739.
  • Ptak, A., Tahchy, A.E., Skrzypek, E., Wójtowicz, T. and Mattar, D.L. 2013. Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus. Cent. Eur. J. Biol. 8(6): 591-599.
  • Sarıkaya, B.B., Somer, N.U. and Onur, M.A. 2012. Quantitative Determinations on Galanthus Rizehensis. Turk J. Pharm. Sci. 9(1):61-66.
  • Vachálková, A., Eisenreichová, E., Haladová, M., Mučaji, P., Jóžová, B. and Novotný, L. 2000. Potencial carcinogenic and inhibitory activity of compounds isolated from Lilium candidum L. Neoplasma. 47(5): 313-318.
  • Zayed, R., El-Shamy, H., Berkov, S., Bastida, J. and Codina, C. 2011. In vitro micropropagation and alkaloids of Hippeastrum Vittatum. In Vitro Cell. Dev. Biol.-Plant. 47: 695-701.

Year 2016, Volume: 13 Issue: 3, 0 - 0, 19.09.2016

Sheida Daneshvar Royandazagh , Elif Ceren Pehlivan

Abstract

References

  • Anonim. Dünya Gıda Dergisi. 2011. (http://www.dunyagida.com.tr/haber.php?nid=3356).
  • Berkov, S., Pavlov, A., Ilieva, M., Burrus, M., Popov, S. and Stanilova, M. 2005. CGC-MS of alkaloids in Leucojum aestivum plants and their in vitro cultures. Phytochem. Anal. 16: 98-103.
  • Bogdanova, Y., Stoeva, T., Yanev, S., Pandova, S., Molle, E., Burrus, M. and Stanilova, M. 2009. Influenceof plant origin on propagation capacity and alkaloid biosynthesis during long- term in vitro cultivation of Leucojum aestivum L. In Vitro Cell. Dev.Biol.-Plant. 45: 458-465.
  • Bogdanova, Y., Pandova, S., Yanev, S. and Stanilova, M. 2014. Biosynthesis of lycorine by in vitro cultures of Pancratium maritimum L. (Amaryllidaceae). Biotechnol. & Biotechnol. Eq. 23(1): 919-922.
  • Cheng, H.D., Jie, G.X., Gen, X.P. and Yong, P. 2013. Phytochemical and biological research of Fritillaria medicinal resources. Chinese Journal of Natural Medicines. 11(4): 330-344.
  • Cupakova, M., Rauova, D., Vitkova, Z., Herdova, P., Grancai, D. and Haladova, M. 2012. Determination of kaempferol in extracts from Lilium Candidum L. Liliaceae by means of liquid chromatography. Acta Fac. Pharm. Uni. Comen. LIX: 14-21.
  • Daneshvar-Royandazagh, S., Pehlivan, E.C., Teykin, E.E. ve Çiftçi, H.S. 2014. Lilium candidum L.’da In Vitro Mikroçoğaltım ile Kozmetik Sanayisine Ham Madde Temini. Turkish Journal of Agricultural and Natural Sciences. Special Issue (2): 1911-1916.
  • Eisenreichova, E., Haladova, M., Buckova, A., Ubik, K. and Uhrin, D. 1991. Derivatives of pyrroline in Lilium candidum L. Chem. Papers. 45(5): 709-711.
  • Eisenreichova, E., Haladova, M., Buckova, A., Tomko, J., Uhrin, D. and Ubik, K. 1992. A pyrroline pyrrolidine alkaloid from Lilium candidum bulbs. Phtochemistry. 31(3): 1084-1085.
  • Eisenreichová, E., Haladová, M., Mučaji, P. and Grančai, D. 2004. The study of constituents of Lilium candidum L. Acta Fac Pharm. Univ Comen. 5: 27-37.
  • Erdoğan, I., Şener, B. and Rahman, A. 2001. Etioline, a steroidal alkaloid from Lilium candidum L. Biochemical Systematics and Ecology. 29: 535-536.
  • Georgiev, V., Ivanov, I., Berkov, S. and Pavlov, A. 2011. Alkaloids biosynthesis by Pancratium maritimum L. shoots in liquid culture. Acta Physiol Plant. 33: 927-933.
  • Georgiev, V., Ivanov, I., Berkov, S., Ilieva, M., Georgiev, M., Gocheva, T. and Pavlov, A. 2012. Galanthamine production by Leucojum aestivum L. shoot culture in a modified bubble column bioreactor with internal sections. Eng. Life Sci. 12(5): 534-543.
  • Haladová, M., Eisenreichová, E., Bůčková, A., Tomko, J. and Uhrín, D. 1988. New nitrogen- containing compounds in Lilium candidum L. Collect. Czechoslov. Chem. Commun. 53: 157-160.
  • Haladova, M., Mucaji, P., Budesinsky, M., Vokac, K., Cvacka, J., Grancai, D. and Eisenreichova, E. 2011. Spirostanol saponins from the bulbs of Lilium candidum. Chemistry of Natural Compounds. 46(6): 1004-1005.
  • Ivanov, I., Georgiev, V., Georgiev, M., Ilieva, M. and Pavlov, A. 2011. Galanthamine and related alkaloids production by Leucojum aestivum L. shoot culture using a temporary immersion technology. Appl Biochem Biotechnol. 163(2): 268- 277.
  • Khadem, S. and Marles, R.J. 2012. Chromone and Flavonoid Alkaloids: Occurrence and Bioactivity. Molecules. 17(1): 191-206.
  • Khawar, K.M., Çöçü, S., Parmaksız, I., Sarıhan, E.O. and Özcan, S. 2005. Mass proliferation of Madonna Lily (Lilium candidum L.) under ın vitro conditions. Pakistan Journal of Botany. 37(2): 243-248.
  • Mammadov, R. 2014. Tohumlu Bitkilerde Sekonder Metabolitler. Nobel Akademik Yayıncılık. Ankara, Türkiye, 412 s.--
  • Mimaki, Y., Satou, T. Kuroda, M., Sashida, Y. and Hatakeyama, Y. 1999. Steroidal saponins from the bulbs of Lilium candidum. Phytochemistry. 51(4): 567-573.
  • Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant. 15(3): 473-497.
  • Ondra, P., Valka, I., Vicar, J., Sütlüpınar, N. and Simanek, V. 1995. Chromatographic determination of constituents of the genus Colchicum (Li!iaceae). Journal of Chromatography A. 704: 351-356.
  • Özuslu, E. and İskender, E. 2009. Geophytes of Sof Mountain (Gaziantep/Turkey). Biological Diversity and Conservation. 2(2): 78-84.
  • Pavlov, A., Berkov, S., Courot, E., Gocheva, T, Tuneva, D, Pandova, B, Georgiev, M, Georgiev, V, Yanev, S, Burrus, M. and Ilieva, M. 2007. Galanthamine production by Leucojum aestivum in vitro systems. Process Biochemistry. 42: 734-739.
  • Ptak, A., Tahchy, A.E., Skrzypek, E., Wójtowicz, T. and Mattar, D.L. 2013. Influence of auxins on somatic embryogenesis and alkaloid accumulation in Leucojum aestivum callus. Cent. Eur. J. Biol. 8(6): 591-599.
  • Sarıkaya, B.B., Somer, N.U. and Onur, M.A. 2012. Quantitative Determinations on Galanthus Rizehensis. Turk J. Pharm. Sci. 9(1):61-66.
  • Vachálková, A., Eisenreichová, E., Haladová, M., Mučaji, P., Jóžová, B. and Novotný, L. 2000. Potencial carcinogenic and inhibitory activity of compounds isolated from Lilium candidum L. Neoplasma. 47(5): 313-318.
  • Zayed, R., El-Shamy, H., Berkov, S., Bastida, J. and Codina, C. 2011. In vitro micropropagation and alkaloids of Hippeastrum Vittatum. In Vitro Cell. Dev. Biol.-Plant. 47: 695-701.
There are 28 citations in total.

Details

Journal Section Articles
Authors

Sheida Daneshvar Royandazagh

Elif Ceren Pehlivan This is me

Publication Date September 19, 2016
Submission Date April 1, 2016
Published in Issue Year 2016 Volume: 13 Issue: 3

Cite

APA Daneshvar Royandazagh, S., & Pehlivan, E. C. (2016). Lilium candidum L.’da In vitro Mikroçoğaltım ve Alkaloidler. Tekirdağ Ziraat Fakültesi Dergisi, 13(3).
AMA Daneshvar Royandazagh S, Pehlivan EC. Lilium candidum L.’da In vitro Mikroçoğaltım ve Alkaloidler. JOTAF. September 2016;13(3).
Chicago Daneshvar Royandazagh, Sheida, and Elif Ceren Pehlivan. “Lilium Candidum L.’da In Vitro Mikroçoğaltım Ve Alkaloidler”. Tekirdağ Ziraat Fakültesi Dergisi 13, no. 3 (September 2016).
EndNote Daneshvar Royandazagh S, Pehlivan EC (September 1, 2016) Lilium candidum L.’da In vitro Mikroçoğaltım ve Alkaloidler. Tekirdağ Ziraat Fakültesi Dergisi 13 3
IEEE S. Daneshvar Royandazagh and E. C. Pehlivan, “Lilium candidum L.’da In vitro Mikroçoğaltım ve Alkaloidler”, JOTAF, vol. 13, no. 3, 2016.
ISNAD Daneshvar Royandazagh, Sheida - Pehlivan, Elif Ceren. “Lilium Candidum L.’da In Vitro Mikroçoğaltım Ve Alkaloidler”. Tekirdağ Ziraat Fakültesi Dergisi 13/3 (September 2016).
JAMA Daneshvar Royandazagh S, Pehlivan EC. Lilium candidum L.’da In vitro Mikroçoğaltım ve Alkaloidler. JOTAF. 2016;13.
MLA Daneshvar Royandazagh, Sheida and Elif Ceren Pehlivan. “Lilium Candidum L.’da In Vitro Mikroçoğaltım Ve Alkaloidler”. Tekirdağ Ziraat Fakültesi Dergisi, vol. 13, no. 3, 2016.
Vancouver Daneshvar Royandazagh S, Pehlivan EC. Lilium candidum L.’da In vitro Mikroçoğaltım ve Alkaloidler. JOTAF. 2016;13(3).