DNA- Binding, Biological Activities, and Chemical Composition of Wild Growing Epilobium angustifolium L. Extracts from Canakkale, Turkey

Year 2017, Volume: 4 Issue: 3, 811 - 840, 03.08.2017

Aysema Sayık Ayşe Sergüzel Yusufoğlu , Leyla Açık , Gülen Türker , Betül Aydın , Leyla Arslan

https://doi.org/10.18596/jotcsa.319789

Cited By: 12

Abstract

The usage of Epilobium
angustifolium L. in folk medicine against prostate and skin diseases encouraged
us to investigate pBR322 DNA-binding ability, biological activities and
chemical composition of wild growing Epilobium
angustifolium L.’s methanolic, ethanolic and aqueous extracts from
Canakkale, Turkey. DNA-binding activity was studied by agarose gel
electrophoresis and chemical composition via GC, GC-MS, HPLC, LC-MS, ICP-MS and
AAS. Literature survey indicates no data on pBR322 plasmid DNA interaction of Epilobium species which is an important
criteria for being a potential anticancer agent. Antibacterial and antifungal
effects were tested against 11 bacterias and 3 fungis and compared with
ampicillin, chloramphenicol and ketokanozol by agar well diffusion techniques. Total
phenolic content was found by Folin-Ciocalteu method and antioxidant activity
was obtained by DPPH and Fe (II)-FZ (ferric-ferrozine)  methods. This plant is rich of flavonoids,  hormones, 
fatty and phenolic acids. Si, K, 
Mg, P and Mn were the main inorganic elements. Epilobium angustifolium L. with its high DNA-binding activity and
rich chemical composition can be a good potential anticancer, antimicrobial and
antioxidant prodrug. 

Keywords

DNA-Binding activity, pBR322 plasmid DNA-Binding, , Epilobium angustifolium L. extracts, cancer therapeutic, chemical analysis¬¬¬

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