Preparation and Cytotoxicity of Coriandrum Sativum L. Oil Loaded Chitosan Nanoparticles

Year 2018, Volume: 5 Issue: 1, 179 - 190, 01.09.2017

Yasemin Budama Kilinc , Rabia Cakir Koc Zeynep Kaya

https://doi.org/10.18596/jotcsa.323798

Cited By: 3

Abstract

Coriandrum sativum L.oil has antibacterial,
antifungal and antimicrobial activity. This
study aims to prepare and characterize chitosan nanoparticles loaded with Coriandrum sativum L., commonly known as coriander oil, using
ionic gelation method and to determine their cytotoxicity in cell culture.
Tripolyphosphate (TPP) was used as a cross-linker in ionic gelation method. The
characterization of the chitosan nanoparticles loaded with coriander (Coriandrum sativum L.) oil was performed
with Zeta-Sizer, Fourier transform Infrared spectrophotometer (FT-IR), scanning
electron microscopy (SEM) and electron dispersive X-ray Spectroscopy (EDS). It
was shown that chitosan nanoparticles loaded with coriander oil have an average
size of 113.5 nm, zeta potential of 16 mV and a polydispersity
index of 0.378. SEM analysis showed that they have spherical
morphology and EDS showed the element composition. Furthermore,
cytotoxicity analysis on L929 fibroblast cells was performed using XTT method
and it was observed that chitosan particles loaded with coriander oil, coriander
oil and empty chitosan nanoparticles did not provoke significant toxicity on
cells. 

Keywords

chitosan, nanoparticle, Coriander (Coriandrum sativum L.), cell culture, cytotoxicity

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