A Facile HPLC-PDA Method for Simultaneous Determination of Paracetamol, Methyl Paraben, Sunset Yellow and Carmosine in Oral Suspensions

Year 2018, Volume: 5 Issue: 2, 763 - 774, 01.01.2018

Şule Dinç Zor , Özlem Aksu Dönmez

https://doi.org/10.18596/jotcsa.403497

Cited By: 2

Abstract

In the present study, a simple, fast
and accurate HPLC-PDA method was developed for the simultaneous determination
of paracetamol (PAR), methylparaben (MP), sunset yellow (SSY) and carmoisine
(CAR) in oral suspensions. The concentrations of colorants are less than with
respect to those of active ingredient and this variation makes process of
analysis toublesome. In the developed HPLC method, efficient chromatographic
separation was achieved using reversed phase C18 column (4.6 mm x 150 mm x 5 μm
particle size) and phosphate buffer solution (pH = 6.5)-acetonitrile mobile
phase with a flow rate of 1.6 mL/min in the gradient mode. The eluents were
monitored via a PDA detector at 300, 254 and 230 nm. The mean retention times of
SSY, PAR, CAR and MP were found to be 1.58, 2.15, 3.81 and 4.42, respectively.
The proposed method was validated in accordance with ICH guidelines and it was
seen that the method met all requirements in terms of linearity, precision,
accuracy and selectivity. The developed method was successfully applied for
simultaneous determination of the studied compounds in two commercial oral
suspension samples. 

Keywords

Paracetamol, methylparaben, sunset yellow, carmoisine HPLC-PDA determination

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