Supercritical Carbondioxide Extraction of Lavandula Officinalis (Lavender) and Hypericum Perforatum (Centaury) Plants Grown in Mersin Region: Investigation of Antioxidant and Antibacterial Activities of Extracts and Usage as Cosmetic Preservatives in Creams
Year 2018,
Volume: 5 Issue: 3, 1215 - 1220, 01.09.2018
Gokturk Avsar
,
Derya Yüksel
Fatih Mehmet Emen
,
Ruken Esra Demirdöğen
,
Tuncay Yeşilkaynak
,
Levent Kahrıman
Abstract
The extraction
of Lavandula Officinalis (lavender) and Hypericum Perforatum (centaury)
plants grown in Mersin region were extracted by supercritical carbondioxide
extraction system (P=100 bar, T=40o C). The chemical composition of
the lavender and centaury extracts were analysed by Gas Chromatography–Mass
Spectrograph (GC-MS). For antioxidant activity experiments, 1,1-diphenyl-2-pycrylhidrazine
(DPPH) radical was used in radical effect tests. For antimicrobial activity studies,
Bacillussubtilis, Klebsiellapneumoniae, Escherichiacoli,
Staphylococcusaureus, Pseudomonasaeruginosa and Streptococcuspneumoniae
with Nutrient Agar Broth (NA) and Eosin Methylene-blue lactose sucrose agar
(EMB) broth were used. For determining antimicrobial effect of plant extracts,
diffusion method was used. Antibacterial and antioxidant properties of the obtained
extracts were examined and have been determined that the resulting extracts
have significant antioxidant and antimicrobial effect. The extracts were also
used in cosmetic cream formulas as protectives. Effective results have also
been determined in antibacterial activity studies of creams after 6 months.
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Year 2018,
Volume: 5 Issue: 3, 1215 - 1220, 01.09.2018
Gokturk Avsar
,
Derya Yüksel
Fatih Mehmet Emen
,
Ruken Esra Demirdöğen
,
Tuncay Yeşilkaynak
,
Levent Kahrıman
References
- 1. Hammer KA, Carson CF, Riley TV. Antimicrobial activity of essential oils and other plant exracts. Jourmal of Applied Microbiology. 1999; (86): 985-90.
- 2. Prabuseenivasan S, Jayakumar M, Ignacimuthu S. In vitro antibacterial activity of some plant essential oils. BMC Complementary and Alternative Medicine. 2006; (6):39.
- 3. Wang L, Weller CL. Recent advances in extraction of nutraceuticals from plants. Trends in Food Science & Technology. 2006; (17): 300–12.
- 4. Zou Y, Lu Y, Wei D. Antioxidant Activity of a Flavonoid-Rich Extract of Hypericum perforatum L. In Vitro. J. Agric. Food Chem. 2004; (52): 5032-9.
- 5. Robu S, Aprotosoaie AC, Miron A, Cioanca O, Stanescu U, Hancianu M. In Vitro Antioxidant Activity of Ethanolic Extracts from Some Lavandula Species Culti Ated in Romania. Farmacia. 2012; (60): 3.
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- 9. Underwood E et al. Ecology of Microorganisms as It Affects the Pharmaceutical Industry. Pharmaceutical Microbiology. 6th ed. Oxford: Blackwell Science. 1998, 339-54.
- 10. Smart R and Spooner DF. Microbiological Spoilage in Pharmaceuticals and Cosmetics. J. Soc. Cosmet. Chem. 1972; (23): 721-37.