EGGSHELL DERIVED NANOHYDROXYAPATITE REINFORCED CHITOSAN CRYOGEL BIOCOMPOSITES FOR TISSUE ENGINEERING APPLICATIONS

Year 2017, Özel Sayı 1, 77 - 88, 20.10.2017

Didem Demir Seda Ceylan Fatma Öfkeli Duygu Şen Nimet Bölgen Karagülle

Abstract

Hydroxyapatite has a biocompatible, biodegradable and natural apatite characteristic to be used in biomedical applications such as bone tissue engineering. The objectives of this study were to synthesize hydroxyapatite from domestic waste eggshells which is utilized as pure calcium source; compare the properties of biosynthesized hydroxyapatite with commercially purchased hydroxyapatite; and produce biosynthesized hydroxyapatite reinforced chitosan cryogels for possible tissue engineering applications. Calcium oxide powders obtained after calcination of waste eggshells showed different particle sizes depending on calcination temperature. It was found that increased temperature of calcination led to the powders of smaller particle sizes. Structural changes at carbonate groups of calcined eggshell were determined by FTIR analysis. The effect of the biosynthesized hydroxyapatite on the morphology of chitosan cryogel biocomposites were determined. The changes in the chemical bond structure of the cryogels were analyzed by FTIR and swelling behavior of produced chitosan cryogels was determined by swelling ratio tests.

Keywords

Biosynthesis, eggshell, hydroxyapatite, chitosan, biocomposite scaffold

References

• 1. Zhou H, Lee J. Nanoscale hydroxyapatite particles for bone tissue engineering. Acta Biomaterialia. 2011 Apr; 7(7): 2769-81.
• 2. Brzezińska-Miecznikn J, Haberko K, Sitarz M, Bućko MM, Macherzyńska B. Hydroxyapatite from animal bones-Extraction and properties. Ceramics International. 2014 Dec; 41(3): 4841-46.
• 3. Rasool T, Ahmed SR, Ather I, Sadia M, Khan R, Jafri AR. Synthesis and Characterization of hydroxyapatite using egg-shell. 2015 Nov; 3: 1-6. (Digests ASME 2015 International Mechanical Engineering Congress and Exposition, Texas 2015).
• 4. Wu SC, Hsu HC, Hsu SK, Chang YC, Ho WF. Synthesis of hydroxyapatite from eggshell powders through ballmilling and heat treatment. Journal of Asian Ceramic Societies. 2015 Dec; 4(1): 85-90.
• 5. Mittal A, Teoti M, Soni RK, Mittal J. Applications of egg shell and egg shell membrane as adsorbents: A review. Journal of Molecular Liquids. Aug 2016; 223: 376-87.
• 6. Padmanabhan SK, Salvatore L, Gervaso F, Catalano M, Taurino A, Sannino A, Licciulli A. Synthesis and characterization of collagen scaffolds reinforced by eggshell derived hydroxyapatite for tissue engineering. Journal of Nanoscience and Nanotechnology. 2015 Sept; 14: 1-6.
• 7. Demir D, Öfkeli F, Ceylan S, Bölgen Karagülle N. Extraction and characterization of chitin and chitosan from blue crab and synthesis of chitosan cryogel scaffolds. Journal of the Turkish Chemical Society, Section A: Chemistry. 2016 Aug; 3(3): 131-44.
• 8. Witoon T. Characterization of calcium oxide derived from waste eggshell and its application as CO2 sorbent. Ceramics International. 2011 May; 37(8): 3291-98.
• 9. Choudhary R, Koppala S, Swamiappan S. Bioactivity studies of calcium magnesium silicate prepared from eggshell waste by sol-gel combustion synthesis. Journal of Asian Ceramic Societies. in press.
• 10. Ummartyotin S, Tangnorawich B. Utilization of eggshell waste as raw material for synthesis of hydroxyapatite. Colloid and Polymer Science. 2015 Jun; 293(9): 2477-83.
• 11. Mornanı EG, Mosayebıan P, Dorranıana D, Behzad K. Effect of calcination temperature on the size and optical properties of synthesized ZnO nanoparticles. Journal of Ovonic Research. 2016 Apr; 12(2): 75-80.
• 12. Gergely G, We´ber F, Luka´cs I, To´th AL, Horva´th ZE, Miha´ly J, Bala´zsi C. Preparation and characterization of hydroxyapatite from eggshell. Ceramics International. 2009 Oct; 36(2): 803-06.
• 13. Chaudhuri B, Mondal B, Modak DK, Pramanik K, Chaudhuri BK. Preparation and characterization of nanocrystalline hydroxyapatite from egg shell and K2HPO4 solution. Materials Letterr. 2013 Jan; 97: 148-50.
• 14. Arsad MSM, Lee PM, Hung LK, "Morphology and particle size analysis of hydroxyapatite micro- and nano-particles," pp. 1030-1034, December 2010 (Digests CSSR 2010 Malaysia, 2010).
• 15. Danilchenko SN, Kalinkevich OV, Pogorelov MV, Kalinkevich AN, Sklyar AM, Kalinichenko TG, Ilyashenko VY, Starikov VV, Bumeyster VI, Sikora VZ, Sukhodub LF, Mamalis AG, Lavrynenko SN, Ramsden JJ. Chitosan-hydroxyapatite composite biomaterials made by a one step co-precipitation method: preparation, characterization and in vivo tests. Journal of Chemical, Biological and Physical Sciences. 2009 Sept; 9(3): 119-26.
• 16. Manjubala I, Scheler S, Bossert J, Jandt KD. Mineralisation of chitosan scaffolds with nano-apatite formation by double diffusion technique. Acta Biomaterialia. 2005 Sept; 2(1): 75-84.