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In vitro Biological Activities and Phytochemical Contents of Portulaca oleracea L. (Purslane)

Year 2021, Volume: 4 Issue: 1, 1 - 7, 02.08.2021

Abstract

P. oleracea is an annual herbaceous plant, belonging to the Portulaceae family. This plant is consumed as a vegetable in Turkey, and is grows spontaneously in wetlands, along streams and meadows. In this study, the antiradical, antimicrobial, anticancer activities and phytochemical contents of aerial parts extracts of P. oleracea were investigated. According to study results, P. oleracea aerial part extracts show very high anticancer activity against MCF-7, HCT-116, A2780 and PC-3 cancer cell lines, high antiradical activity against OH radicals, and effective antimicrobial activity against some microorganisms. Also, it was determined that P. oleracea aerial parts contain highly phytochemical compounds. In conclusion, it can be said that the P. oleracea aerial parts can be used for antiradical, antimicrobial, anticancer purposes.

Supporting Institution

TÜBİTAK

Project Number

115Z056

Thanks

The present study was supported by TUBITAK, under grant number 115Z056.

References

  • [1] Ç. Kaşar, “İzmir ve çevresindeki semizotu (Portulaca oleracea L.) genotiplerinin bazı morfolojik özellikleri ve moleküler karakterizasyonu” (In Turkish), Master Thesis, Ege University, Institute of Science, 2009.
  • [2] P.Z. Akdeniz, “Semizotunda (Portulaca oleracea L.) farklı saklama koşullarının kalite ve besin içeriği üzerine etkileri” (In Turkish), Master Thesis, Ege University, Institute of Science, 2007.
  • [3] A. Peksel, I. Arisan-Atac, R. Yanardag, “Antioxidant activities of aqueous extracts of purslane (Portulaca oleracea subsp. sativa L.)” Italian Journal of Food Science, 18, 295-308, 2006.
  • [4] Y.Y. Lim, E.P.L. Quah, “Antioxidant properties of different cultivars of Portulaca oleracea” Food Chemistry, 103, 734-740, 2007.
  • [5] S. Ercisli, I. Coruh, A. Gomez, M. Sengul, “Antioxidant and antibacterial activities of Portulaca oleracea L. grown wild in Turkey” Italian Journal of Food Science, 20, 533-542, 2008.
  • [6] Y.J. Cho, I.S. Ju, O.J. Kwon, S.S. Chun, B.J. An, J.H. Kim, “Biological and antimicrobial activity of Portulaca oleracea” Journal of the Korean Society for Applied Biological Chemistry, 51, 49-54, 2008.
  • [7] I. Oliveira, P. Valentao, R. Lopes, P.B. Andrade, A. Bento, J.A. Pereira, 2009. “Phytochemical characterization and radical scavenging activity of Portulaca oleracea L. leaves and stems” Microchemical Journal, 92, 129-134, 2009.
  • [8] S. Siriamornpun, M. Suttajit, “Microchemical components and antioxidant activity of different morphological plants of thai wild purslane (Portulaca oleracea)” Weed Science, 58, 182-188, 2010.
  • [9] M. Boga, I. Hacibekiroglu, U. Kolak, “Antioxidant and anticholinesterase activities of eleven edible plants” Pharmaceutical Biology, 49, 290-295, 2011.
  • [10] M.A. Dkhil, A.E.A. Moniem, S. Al-Quraishy, R.A. Saleh, “Antioxidant effect of purslane (Portulaca oleracea) and its mechanism of action” Journal of Medicinal Plants Research, 5, 1584-1593, 2011.
  • [11] N. Erkan, “Antioxidant activity and phenolic compounds of fractions from Portulaca oleracea L.” Food Chemistry, 133, 775-781, 2012.
  • [12] M.K. Uddin, A.S. Juraimi, M.S. Hossain, M.A.U. Nahar, M.E. Ali, M.M. Rahman, “Purslane weed (Portulaca oleracea): A prospective plant source of nutrition, omega-3 fatty acid, and antioxidant attributes” The Scientific World Journal, Article ID 951019, 6 pages, 2014.
  • [13] M.A. Alam, A.S. Juraimi, M.Y. Rafii, A.A. Hamid, F. Aslani, M.M. Hasan, M.A.M. Zainudin, M.K. Uddin, “Evaluation of antioxidant compounds, antioxidant activities, and mineral composition of 13 collected pusrlane (Portulaca oleracea L.) accessions” Biomed Research International, Article ID 296063, 10 pages, 2014.
  • [14] Y. Li, Y. Hu, S. Shi, L. Jiang, “Evaluation of antioxidant and immuno-enhancing activities of purslane polysaccharides in gastric cancer rats” International Journal of Biological Macromolecules, 68, 113-116, 2014.
  • [15] R. Silva, I.S. Carvalho, “In vitro antioxidant activity, phenolic compounds and protective effect against DNA damage provided by leaves, stems and flowers of Portulaca oleracea (purslane)” Natural Product Communications, 9, 45-50, 2014.
  • [16] G. Karimi, A. Khoei, A. Omidi, M. Kalantari, J. Babaei, E. Taghiabadi, B.M. Razavi, “Protective effect of aqueous and ethanolic extracts of Portulaca oleracea against cisplatin induced nephrotoxicity” Iranian Journal of Basic Medical Sciences, 13, 31-35, 2010.
  • [17] G.C.S. Tan, K.M. Wong, G.Q.P. Wong, S.L. Yeo, S.K. Yeap, B.C. Yiap, H.Z. Chang, “In vitro cytotoxic and antiproliferative effects of Portulaca oleracea methanol extracts on breast, cervical, colon and nasopharyngeal cancerous cell lines” Sains Malaysiana, 42, 927-935, 2013.
  • [18] R. Zhao, X. Gao, Y. Cai, X. Shao, G. Jia, Y. Huang, X. Qin, J. Wang, X. Zheng, “Antitumor activity of Portulaca oleracea L. polysaccharides against cervical carcinoma in vitro and in vivo” Carbohydrate Polymers, 96, 376-383, 2013.
  • [19] S. Keser, “Antiradical activities and phytochemical compounds of firethorn (Pyracantha coccinea) fruit extracts” Natural Product Research, 28, 1789-1794, 2014.
  • [20] W. Brand-Williams, M.E. Cuvelier, C. Berset, “Use of a free radical method to evaluate antioxidant activity” LWT-Food Science and Technology, 28, 25-30, 1995.
  • [21] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, “Antioxidant activity applying an improved ABTS radical cation decolorization assay” Free Radical Biology and Medicine, 26, 1231-1237, 1999.
  • [22] B. Halliwell, J.M.C. Gutteridge, O. Aruoma, “The deoxyribose method: a simple test tube assay for determination of rate constants for reactions of hydroxyl radicals” Analytical Biochemistry, 165, 215-219, 1987.
  • [23] K. Slinkard, V.L. Singleton, “Total phenol analysis-automation and comparison with manual methods” American Journal of Enology and Viticulture, 28, 49-55, 1977.
  • [24] D.O. Kim, O.K. Chun, Y.J. Kim, H.Y. Moon, C.Y. Lee, “Quantification of polyphenolics and their antioxidant capacity in fresh plums” Journal of Agricultural and Food Chemistry, 51, 6509-6515, 2003.
  • [25] O.U. Amaeze, G.A. Ayoola, M.O. Sofidiya, A.A. Adepoju-Bello, A.O. Adegoke, H.A.B. Coker, “Evaluation of antioxidant activity of Tetracarpidium conophorum (Mull. Arg) Hutch & Dalziel leaves” Oxidative Medicine and Cellular Longevity, Article ID 976701, 7 pages, 2011.
  • [26] Y.G. Zu, C.Y. Li, Y.J. Fu, C.J. Zhao, “Simultaneous determination of catechin, rutin, quercetin, kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaf by RP-HPLC with DAD” Journal of Pharmaceutical and Biomedical Analysis, 41, 714-719, 2006.
  • [27] W.W. Christie, “Gas chromatography and lipids” The Oil Press, Glasgow, 1992.
  • [28] D.I. Sanchez-Machado, J. Lopez-Hernandez, P. Paseiro-Losado, “High performance liquid chromatographic determination of alpha-tocopherol in macroalgae” Journal of Chromatography A, 976, 277-284, 2002.
  • [29] J. López-Cervantes, D.I. Sánchez-Machado, N.J. Ríos-Vázquez, “High performance liquid chromatography method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate” Journal of Chromatography A, 1105, 135-139, 2006.
  • [30] C.M. Collins, P.M. Lyne, “Microbiological methods” Buttermorths-Heinemann, London, England, 1989.
  • [31] T. Mosmann, “Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays” Journal of Immunological Methods, 65, 55-63, 1983.
  • [32] F. Denizot, R. Lang, “Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability” Journal of Immunological Methods, 89, 271-277, 1986.
  • [33] S.A. Petropoulos, A. Karkanis, A. Fernandes, L. Barros, I.C.F.R. Ferreira, G. Ntatsi, K. Petrotos, C. Lykas, E. Khah, “Chemical composition and yield of six genotypes of common purslane (Portulaca oleracea L.): An alternative source of omega-3 fatty acids” Plant Foods for Human Nutrition, 70, 420-426, 2015.
  • [34] M. Marrelli, B. Cristaldi, F. Menichini, F. Conforti, “Inhibitory effects of wild dietary plants on lipid peroxidation and on the proliferation of human cancer cells” Food and Chemical Toxicology, 86, 16-24, 2015.
  • [35] A.P. Simopoulos, H.A. Norman, J.E. Gillaspy, J.A. Duker, “Common purslane: a source of omega-3 fatty acids and antioxidants” Journal of the American College of Nutrition, 11, 374-382, 1992.
  • [36] A.P. Simopoulos, Jr. N. Salem, “Purslane: a terrestrial source of omega-3 fatty acids” The New England Journal of Medicine, 315, 833-833, 1986.
  • [37] T.R. Omara-Alwara, T. Mebrahtu, T.E. Prior, M.O. Ezekwe, “Omega-three fatty acids in purslane (Portulaca oleracea) tissues” Journal of the American Oil Chemists’ Society, 68, 198-199, 1991.
  • [38] E.S. Elkhayat, S.R.M. Ibrahim, M.A. Aziz, “Portulene, a new diterpene from Portulaca oleracea L.” Journal of Asian Natural Product Research, 10, 1039-1043, 2008.
Year 2021, Volume: 4 Issue: 1, 1 - 7, 02.08.2021

Abstract

Project Number

115Z056

References

  • [1] Ç. Kaşar, “İzmir ve çevresindeki semizotu (Portulaca oleracea L.) genotiplerinin bazı morfolojik özellikleri ve moleküler karakterizasyonu” (In Turkish), Master Thesis, Ege University, Institute of Science, 2009.
  • [2] P.Z. Akdeniz, “Semizotunda (Portulaca oleracea L.) farklı saklama koşullarının kalite ve besin içeriği üzerine etkileri” (In Turkish), Master Thesis, Ege University, Institute of Science, 2007.
  • [3] A. Peksel, I. Arisan-Atac, R. Yanardag, “Antioxidant activities of aqueous extracts of purslane (Portulaca oleracea subsp. sativa L.)” Italian Journal of Food Science, 18, 295-308, 2006.
  • [4] Y.Y. Lim, E.P.L. Quah, “Antioxidant properties of different cultivars of Portulaca oleracea” Food Chemistry, 103, 734-740, 2007.
  • [5] S. Ercisli, I. Coruh, A. Gomez, M. Sengul, “Antioxidant and antibacterial activities of Portulaca oleracea L. grown wild in Turkey” Italian Journal of Food Science, 20, 533-542, 2008.
  • [6] Y.J. Cho, I.S. Ju, O.J. Kwon, S.S. Chun, B.J. An, J.H. Kim, “Biological and antimicrobial activity of Portulaca oleracea” Journal of the Korean Society for Applied Biological Chemistry, 51, 49-54, 2008.
  • [7] I. Oliveira, P. Valentao, R. Lopes, P.B. Andrade, A. Bento, J.A. Pereira, 2009. “Phytochemical characterization and radical scavenging activity of Portulaca oleracea L. leaves and stems” Microchemical Journal, 92, 129-134, 2009.
  • [8] S. Siriamornpun, M. Suttajit, “Microchemical components and antioxidant activity of different morphological plants of thai wild purslane (Portulaca oleracea)” Weed Science, 58, 182-188, 2010.
  • [9] M. Boga, I. Hacibekiroglu, U. Kolak, “Antioxidant and anticholinesterase activities of eleven edible plants” Pharmaceutical Biology, 49, 290-295, 2011.
  • [10] M.A. Dkhil, A.E.A. Moniem, S. Al-Quraishy, R.A. Saleh, “Antioxidant effect of purslane (Portulaca oleracea) and its mechanism of action” Journal of Medicinal Plants Research, 5, 1584-1593, 2011.
  • [11] N. Erkan, “Antioxidant activity and phenolic compounds of fractions from Portulaca oleracea L.” Food Chemistry, 133, 775-781, 2012.
  • [12] M.K. Uddin, A.S. Juraimi, M.S. Hossain, M.A.U. Nahar, M.E. Ali, M.M. Rahman, “Purslane weed (Portulaca oleracea): A prospective plant source of nutrition, omega-3 fatty acid, and antioxidant attributes” The Scientific World Journal, Article ID 951019, 6 pages, 2014.
  • [13] M.A. Alam, A.S. Juraimi, M.Y. Rafii, A.A. Hamid, F. Aslani, M.M. Hasan, M.A.M. Zainudin, M.K. Uddin, “Evaluation of antioxidant compounds, antioxidant activities, and mineral composition of 13 collected pusrlane (Portulaca oleracea L.) accessions” Biomed Research International, Article ID 296063, 10 pages, 2014.
  • [14] Y. Li, Y. Hu, S. Shi, L. Jiang, “Evaluation of antioxidant and immuno-enhancing activities of purslane polysaccharides in gastric cancer rats” International Journal of Biological Macromolecules, 68, 113-116, 2014.
  • [15] R. Silva, I.S. Carvalho, “In vitro antioxidant activity, phenolic compounds and protective effect against DNA damage provided by leaves, stems and flowers of Portulaca oleracea (purslane)” Natural Product Communications, 9, 45-50, 2014.
  • [16] G. Karimi, A. Khoei, A. Omidi, M. Kalantari, J. Babaei, E. Taghiabadi, B.M. Razavi, “Protective effect of aqueous and ethanolic extracts of Portulaca oleracea against cisplatin induced nephrotoxicity” Iranian Journal of Basic Medical Sciences, 13, 31-35, 2010.
  • [17] G.C.S. Tan, K.M. Wong, G.Q.P. Wong, S.L. Yeo, S.K. Yeap, B.C. Yiap, H.Z. Chang, “In vitro cytotoxic and antiproliferative effects of Portulaca oleracea methanol extracts on breast, cervical, colon and nasopharyngeal cancerous cell lines” Sains Malaysiana, 42, 927-935, 2013.
  • [18] R. Zhao, X. Gao, Y. Cai, X. Shao, G. Jia, Y. Huang, X. Qin, J. Wang, X. Zheng, “Antitumor activity of Portulaca oleracea L. polysaccharides against cervical carcinoma in vitro and in vivo” Carbohydrate Polymers, 96, 376-383, 2013.
  • [19] S. Keser, “Antiradical activities and phytochemical compounds of firethorn (Pyracantha coccinea) fruit extracts” Natural Product Research, 28, 1789-1794, 2014.
  • [20] W. Brand-Williams, M.E. Cuvelier, C. Berset, “Use of a free radical method to evaluate antioxidant activity” LWT-Food Science and Technology, 28, 25-30, 1995.
  • [21] R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans, “Antioxidant activity applying an improved ABTS radical cation decolorization assay” Free Radical Biology and Medicine, 26, 1231-1237, 1999.
  • [22] B. Halliwell, J.M.C. Gutteridge, O. Aruoma, “The deoxyribose method: a simple test tube assay for determination of rate constants for reactions of hydroxyl radicals” Analytical Biochemistry, 165, 215-219, 1987.
  • [23] K. Slinkard, V.L. Singleton, “Total phenol analysis-automation and comparison with manual methods” American Journal of Enology and Viticulture, 28, 49-55, 1977.
  • [24] D.O. Kim, O.K. Chun, Y.J. Kim, H.Y. Moon, C.Y. Lee, “Quantification of polyphenolics and their antioxidant capacity in fresh plums” Journal of Agricultural and Food Chemistry, 51, 6509-6515, 2003.
  • [25] O.U. Amaeze, G.A. Ayoola, M.O. Sofidiya, A.A. Adepoju-Bello, A.O. Adegoke, H.A.B. Coker, “Evaluation of antioxidant activity of Tetracarpidium conophorum (Mull. Arg) Hutch & Dalziel leaves” Oxidative Medicine and Cellular Longevity, Article ID 976701, 7 pages, 2011.
  • [26] Y.G. Zu, C.Y. Li, Y.J. Fu, C.J. Zhao, “Simultaneous determination of catechin, rutin, quercetin, kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaf by RP-HPLC with DAD” Journal of Pharmaceutical and Biomedical Analysis, 41, 714-719, 2006.
  • [27] W.W. Christie, “Gas chromatography and lipids” The Oil Press, Glasgow, 1992.
  • [28] D.I. Sanchez-Machado, J. Lopez-Hernandez, P. Paseiro-Losado, “High performance liquid chromatographic determination of alpha-tocopherol in macroalgae” Journal of Chromatography A, 976, 277-284, 2002.
  • [29] J. López-Cervantes, D.I. Sánchez-Machado, N.J. Ríos-Vázquez, “High performance liquid chromatography method for the simultaneous quantification of retinol, α-tocopherol, and cholesterol in shrimp waste hydrolysate” Journal of Chromatography A, 1105, 135-139, 2006.
  • [30] C.M. Collins, P.M. Lyne, “Microbiological methods” Buttermorths-Heinemann, London, England, 1989.
  • [31] T. Mosmann, “Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays” Journal of Immunological Methods, 65, 55-63, 1983.
  • [32] F. Denizot, R. Lang, “Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability” Journal of Immunological Methods, 89, 271-277, 1986.
  • [33] S.A. Petropoulos, A. Karkanis, A. Fernandes, L. Barros, I.C.F.R. Ferreira, G. Ntatsi, K. Petrotos, C. Lykas, E. Khah, “Chemical composition and yield of six genotypes of common purslane (Portulaca oleracea L.): An alternative source of omega-3 fatty acids” Plant Foods for Human Nutrition, 70, 420-426, 2015.
  • [34] M. Marrelli, B. Cristaldi, F. Menichini, F. Conforti, “Inhibitory effects of wild dietary plants on lipid peroxidation and on the proliferation of human cancer cells” Food and Chemical Toxicology, 86, 16-24, 2015.
  • [35] A.P. Simopoulos, H.A. Norman, J.E. Gillaspy, J.A. Duker, “Common purslane: a source of omega-3 fatty acids and antioxidants” Journal of the American College of Nutrition, 11, 374-382, 1992.
  • [36] A.P. Simopoulos, Jr. N. Salem, “Purslane: a terrestrial source of omega-3 fatty acids” The New England Journal of Medicine, 315, 833-833, 1986.
  • [37] T.R. Omara-Alwara, T. Mebrahtu, T.E. Prior, M.O. Ezekwe, “Omega-three fatty acids in purslane (Portulaca oleracea) tissues” Journal of the American Oil Chemists’ Society, 68, 198-199, 1991.
  • [38] E.S. Elkhayat, S.R.M. Ibrahim, M.A. Aziz, “Portulene, a new diterpene from Portulaca oleracea L.” Journal of Asian Natural Product Research, 10, 1039-1043, 2008.
There are 38 citations in total.

Details

Primary Language English
Subjects Chemical Engineering
Journal Section Articles
Authors

Fatma Keser 0000-0001-6870-0546

Mustafa Karatepe 0000-0001-6358-5913

Serhat Keser 0000-0002-9678-1053

Suat Tekin 0000-0002-2757-1802

İsmail Türkoğlu 0000-0001-7454-7605

Omer Kaygili 0000-0002-2321-1455

Ersin Demir 0000-0002-7676-5953

Prof. Dr. Ökkeş Yılmaz 0000-0002-8276-4498

Süleyman Sandal 0000-0002-8916-3329

Sevda Kırbag 0000-0002-4337-8236

Project Number 115Z056
Publication Date August 2, 2021
Submission Date April 17, 2021
Acceptance Date May 3, 2021
Published in Issue Year 2021 Volume: 4 Issue: 1

Cite

APA Keser, F., Karatepe, M., Keser, S., Tekin, S., et al. (2021). In vitro Biological Activities and Phytochemical Contents of Portulaca oleracea L. (Purslane). Journal of Physical Chemistry and Functional Materials, 4(1), 1-7.
AMA Keser F, Karatepe M, Keser S, Tekin S, Türkoğlu İ, Kaygili O, Demir E, Yılmaz PDÖ, Sandal S, Kırbag S. In vitro Biological Activities and Phytochemical Contents of Portulaca oleracea L. (Purslane). Journal of Physical Chemistry and Functional Materials. August 2021;4(1):1-7.
Chicago Keser, Fatma, Mustafa Karatepe, Serhat Keser, Suat Tekin, İsmail Türkoğlu, Omer Kaygili, Ersin Demir, Prof. Dr. Ökkeş Yılmaz, Süleyman Sandal, and Sevda Kırbag. “In Vitro Biological Activities and Phytochemical Contents of Portulaca Oleracea L. (Purslane)”. Journal of Physical Chemistry and Functional Materials 4, no. 1 (August 2021): 1-7.
EndNote Keser F, Karatepe M, Keser S, Tekin S, Türkoğlu İ, Kaygili O, Demir E, Yılmaz PDÖ, Sandal S, Kırbag S (August 1, 2021) In vitro Biological Activities and Phytochemical Contents of Portulaca oleracea L. (Purslane). Journal of Physical Chemistry and Functional Materials 4 1 1–7.
IEEE F. Keser, “In vitro Biological Activities and Phytochemical Contents of Portulaca oleracea L. (Purslane)”, Journal of Physical Chemistry and Functional Materials, vol. 4, no. 1, pp. 1–7, 2021.
ISNAD Keser, Fatma et al. “In Vitro Biological Activities and Phytochemical Contents of Portulaca Oleracea L. (Purslane)”. Journal of Physical Chemistry and Functional Materials 4/1 (August 2021), 1-7.
JAMA Keser F, Karatepe M, Keser S, Tekin S, Türkoğlu İ, Kaygili O, Demir E, Yılmaz PDÖ, Sandal S, Kırbag S. In vitro Biological Activities and Phytochemical Contents of Portulaca oleracea L. (Purslane). Journal of Physical Chemistry and Functional Materials. 2021;4:1–7.
MLA Keser, Fatma et al. “In Vitro Biological Activities and Phytochemical Contents of Portulaca Oleracea L. (Purslane)”. Journal of Physical Chemistry and Functional Materials, vol. 4, no. 1, 2021, pp. 1-7.
Vancouver Keser F, Karatepe M, Keser S, Tekin S, Türkoğlu İ, Kaygili O, Demir E, Yılmaz PDÖ, Sandal S, Kırbag S. In vitro Biological Activities and Phytochemical Contents of Portulaca oleracea L. (Purslane). Journal of Physical Chemistry and Functional Materials. 2021;4(1):1-7.