Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model

Mehmet Mustafa Demir; Volkan İpek; Hidayet Tutun; Harun Çınar; Leyla Elif Özgü Ayözger

doi:10.30782/jrvm.1217557

Research Article

Effects of Salvia Tomentosa Ointment on Dermal Wound Healing in a Rabbit Model

Year 2023, , 54 - 64, 31.12.2023

Mehmet Mustafa Demir , Volkan İpek , Hidayet Tutun , Harun Çınar , Leyla Elif Özgü Ayözger

https://doi.org/10.30782/jrvm.1217557

Abstract

This study aimed to evaluate the wound healing activity of an ointment containing the hydroethanolic extract of Salvia Tomentosa (S. tomentosa)
in a dermal wound model using rabbits. Ointments with concentrations of 2.5% (w/v) and 5% (w/v) were prepared using the hydroethanolic
extracts of S. tomentosa. An in vivo excisional wound model was embloyed with New Zealand rabbits (n = 35) to assess the wound healing
activity of these ointments. Rabbits were randomly divided into five experimental groups: negative (received no treatment), vehicle (received
only the ointment vehicle), positive control (treated with Fitokrem®), and those treated with 2.5% (w/v) and 5% (w/v) S. tomentosa ointments.
Wound diameters were measured using calipers on the 4th, 8th, and 14th days, and histopathological examinations were conducted on the 3rd,
7th, and 14th days of treatment. The total phenolic content of the S. tomentosa hydroethanolic extract was determined to be 61.52 ± 2.33 μg of
Gallic Acid Equivalents per mg of dry plant material. The total phenolic content and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity
of the hydroethanolic extract of Salvia tomentosa were determined spectrophotometrically. In terms of wound closure, both
2.5% and 5% S. tomentosa ointments demonstrated wound healing activity comparable to that of Fitokrem®. In conclusion, S. tomentosa exibits a
positive effect on dermal wound healing in rabbits.

Keywords

Rabbit, radical scavenging activity, Salvia tomentosa, total phenolic content, wound healing

Supporting Institution

Burdur Mehmet Akif Ersoy University, Scientific Research Project Office

Project Number

0644-YL-20

Thanks

This work was supported by Burdur Mehmet Akif Ersoy University, Scientific Research Project Office under Grant no. 0644-YL-20. We thank to Prof Dr Osman TUGAY for the herbarium number, and Dr Ümit ERDOĞAN for the ointment preparation and Kale Naturel for the extraction process.

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