The Study of Histopathologic Changes of Experimental Infection with Listonella (Vibrio) anguillarum in Rainbow Trout

Year 2019, , 59 - 66, 18.07.2019

Mustafa Ceylan Muhammed Duman Sevda Inan M. Ozgur Ozyıgıt , İzzet B. Satıcıoglu Soner Altun

https://doi.org/10.30782/uluvfd.423345

Cited By: 1

Abstract

The rainbow trout production increased more than 100% in the last decade and total rainbow trout production was shown as 107.013 tons

according to 2016 statics. According to Federation of European Aquaculture Producer, Turkey was determined as the biggest rainbow trout

producer into the European Countries in 2016. Together with high production capacity, a number of outbreaks were reported causing L.

anguillarum (Vibriosis). The pathogenesis of L. anguillarum which were experimentally infected in rainbow trout were examined during 15

days in our study. Spreading of agent that was injected with intraperitoneally into tissue and organs were studied by using histopathological

methods. The mortality rate of agent was determined above 70% and deaths were seen in 2-3th days of experiment. In addition of these, liver,

spleen, kidney and gills were determined as the most affected organs and tissues. In the present study was obtain for original pathological

findings of L. anguillarum.

Keywords

L. anguillarum, Vibriosis, Pathology, Rainbow trout

References

• 1. Austin B, Austin DA. Characteristics of the pathogens: Gram-negative bacteria. In: Bacterial fish pathogens: disease in farmed and wild fish. (Eds.: B. Austin. and D.A. Austin). Third (revised) edition. Praxis Publishing Ltd., pp. 102-118, New York, 1999.
• 2. Toranzo AE, Magariňos B, Romalde JL. A review of the main bacterial fish diseases in mariculture systems. Aquaculture, 246, 31-67, 2005.
• 3. Candan A. Çipura (Sparus aurata L.1758) Balıklarında Vibrio anguillarum Enfeksiyonu. Türk Mikrob Cem Derg, 23, 25-27, 1993.
• 4. Korun J. Kültürü yapılan Çipuralarda (Sparus aurata L.) görülen Listonella anguillarum enfeksiyonu üzerine bir çalışma, Ege Ün. Su Ürü Derg, 23: 259-263, 2006.
• 5. Krovacek K, Faris A, Mansson I. Cytotoxic and skin permeability factors produced by Vibrio anguillarum. Aquaculture, 67, 87-91, 1987.
• 6. Giorgetti G, Ceschia G. Vibriosis in rainbow trout, Salmo gairdneri Richardson, in fresh water in North-eastern Italy, J of Fish Dis, 5: 125-130, 1982.
• 7. Irie T, Watarai S, Iwasaki T, Kodama H. Binding of Vibrio anguillarum to neutral glycosphingolipids from intestinal mucosa of rainbow trout (Oncorhynchus mykiss), J of Vet Med Sc, 66: 205-208, 2004.
• 8. Hickey, Michael E.; Lee, Jung‐Lim. A comprehensive review of Vibrio (Listonella) anguillarum: ecology, pathology and prevention. Rev in Aqua, 10.3: 585-610, 2018.
• 9. Boesen HT, Pedersen K, Larsen JL, Koch C, Ellis AE. Vibrio anguillarum resistance to rainbow trout (Oncorhynchus mykiss) serum: Role of O- antigen structure of lipopolysaccharide, Infec and Immunity, 67: 294- 301, 1999.
• 10. McCarthy DH, Stevenson JP, Roberts MS. Vibriosis in rainbow trout. J of Wildlife Dis, 10: 2-7, 1974.
• 11. Avci H, Birincioğlu S, Çagirgan H. Morphologic and immunohistochemical investigations in rainbow trout (O. mykiss Walbaum, 1972) experimentally infected with Vibrio anguillarum, Revue Med Vet, 163 ,1 ,31- 39, 2012.
• 12. Engelsen AR, Sandlund N, Fiksdal IU, Bergh O. Immunohistochemistry of Atlantic cod larvae Gadus morhua experimentally challenged with Vibrio anguillarum. Dis Aquat Org 80:13–20, 2008.
• 13. Egidius M. Vibriosis: pathogenicity and pathology. A review, Aquaculture, 67: 15-28, 1987.
• 14. Ekici S, Diler Ö, Altun S. Kültürü yapılan balıklarda görülen Vibrio enfeksiyonu. Ulusal Su Ürünleri Sempozyumu. 1-4 Eylül 2005 Çanakkale 18 Mart Üniv., 50s. Çanakkale
• 15. Spanggaard B, Huber I, Nielsen T, Gram L. Proliferation and location of Vibrio anguillarum during infection of rainbow trout, Oncorhynchus mykiss (Walbaum), J of Fish Dis, 23: 423-427, 2000.
• 16. Laurencin FB, Germon E. Experimental infection of rainbow trout, Salmo gairdneri R., by dipping in suspension of Vibrio anguillarum: ways of bacterial penetration; influence of temperature and salinity, Aquaculture, 67: 203-272, 1987.
• 17. Hansen GH, Olafsen JA. Bacterial Colonization of Cod (Gadus morhua L.) and Halibut (Hippoglossus hippoglossus) Eggs in Marine Aquaculture. Appl Environ Microbiol. 55(6):1435–1446, 1989.
• 18. Austin B, Austin DA. Bacterial Fish Pathogens. (Eds.: B. Austin. and D.A. Austin). 5th ed., XXXIII, 652 p, 2012.
• 19. Ceylan M, and ALTUN S. Vibrio Anguillarum İle İnfekte Edilmiş Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) Hematolojik İncelemeler. Uludağ Üni Vet Fak Derg, 29(2), 2010.
• 20. De Kinkelin P, Michel C, Ghittino P. Précis de pathologie des poissons. Office International des Epizooties et Institut National de la Recherche Agronomique Editeurs. Paris. 348 pp, 1986.
• 21. Altun S, Diler Ö. Yersinia ruckeri ile infekte edilmiş gökkuşağı alabalıklarında (Oncorhynchus mykiss) hematolojik incelemeler. Turk J of Vet and Anim Sci, 23: 301-309, 1996.
• 22. Altun S, Adiloğlu AK, Kubilay A, Diler Ö, Delibaş N, Sütçü R. Immunogenic and Antigenic Profiles of Nine Lactococcus garvieae Strains from Different Rainbow Trout Farms. The Israeli J of Aqua, 59(2): 111-116, 2007.
• 23. Kubilay A, Altun S, Uluköy G, Ekici S, Diler Ö. Immunization of rainbow trout (Oncorhynchus mykiss) against Lactococcus garvieae using vaccine mixtures. The Israeli J of Aqua, 60(4), 265-270, 2008.
• 24. Zhaolan M, Dongsheng G, Yunxiang M, Xuhong Y, Yuxia Z, Peng X, Bin H. Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease. Chinese J of Ocea and Limno. 28(1), 55-61, 2010.
• 25. Egidius EC, Andersen K. Host-specific pathogenicity of strains of Vibrio anguillarum isolated from rainbow trout Salmo gairdneri Richardson and saithe Pollachius virens (L.), J of Fish Dis, 1: 45-50, 1978.
• 26. Tanrikul TT. Vibriosis as an epizootic disease of rainbow trout (Oncorhynchus mykiss) in Turkey, Pakistan J of Bio Sci, 10: 1733-1737, 2007.
• 27. Kanno T, Nakai T, Muroga K. Scanning electron microscopy on the skin surface of ayu Plecoglossus altivelis infected with Vibrio anguillarum, Dis Aquat Org, 8: 73-75, 1990.
• 28. Roed KH, Fevolden SE, Fjalestad KT. Disease resistance and immune characteristics in rainbow trout (Oncorhynchus mykiss) selected for lysozyme activity, Aquaculture, 209: 91-101, 2002.
• 29. Johnson KA, Amend DF. Efficacy of Vibrio anguillarum and Yersinia ruckeri bacterins applied by oral and anal intubation of salmonids, J of Fish Dis, 6: 473-476, 1983.
• 30. Ransom DP, Lannan CN, Rohovec JS, Fryer JL. Comprasion of histopathology caused by Vibrio anguillarum and Vibrio ordalii in three species of Pacific salmons. J Fish Dis, 7, 107-115, 1984.
• 31. Lönnström LG, Rahkonen R, Lunden T, Pasternack M, Koskela J, Gröndahl A. Protection, immune response and side-effects in European whitefish (Coregonus lavaretus L.) vaccinated against vibriosis and furunculosis. Aquaculture, 200, 271-284, 2001.
• 32. Cagirgan H. Vaccine development in sea bass fry (Dicentrarchus labrax L., 1758) against vibriosis. Europ. J Fish Aquat Sci., 21, 271-274, 2004.
• 33. Austin B, Austin DA. Enterobacteriaceae Representatives, in: Bact Fish Pathog, Springer International Publishing, Cham, pp. 323–396. doi:10.1007/978-3-319- 32674-0_6, 2016.
• 34. Hacking MA, Budd J. Vibrio infection in tropical fish in a freshwater aquarium, J of Wildlife Dis, 7: 273-280, 1971.
• 35. Horne MT, Richards RH, Roberts RJ, Smith PC. Peracute vibriosis in juvenile turbot Scophthalmus maximus, J of Fish Bio, 11: 355-361, 1977.
• 36. Jones M, Cockerill DJ, Birkbeck TH, Cox DI. Clinical infection of cod (Gadus morhua L.) in Scotland by Vibrio anguillarum-a case, Bull of the Euro Ass of Fish Path, 20: 125-128, 2000.
• 37. Lewis DH. Vibriosis in channel catfish, Ictalurus punctatus (Rafinesque), J of Fish Dis, 8: 539-545, 1985. 38. Roberts RJ. Fish Pathology, 3rd Ed., W.B. Saunders, Toronto, 2001.
• 39. Olsson JC, Joborn A, Westerdahl A, Blomberg L, Kjelleberg S, Conway PL. Is the turbot, Scophthalmus maximus (L), intestines a portal of entry for the fish pathogen Vibrio anguillarum, J of Fish Dis, 19: 225- 234, 1996.
• 40. Miyazaki T. A histological study of the response to challenge with vibriosis in ayu, Plecoglossus altivelis Temminck and Schlegel, vaccinated by immersion and injection with Vibrio anguillarum bacterin, J of Fish Dis, 10: 445-452, 1987.