Acute Effect of Centrally Injected Nesfatin-1 on Some Blood Electrolytes and Metabolites in Rats

Year 2020, , 149 - 152, 30.12.2020

Gökçen Güvenç Bayram , Ebru Yalçın Ülger , Murat Yalçın

https://doi.org/10.30782/jrvm.735993

Abstract

Nesfatin-1 is a newly found food and water intake regulatory neuropeptide. Because it can regulate nutrition and thirst, nesfatin-1 may also have the potential to affect levels of blood electrolytes and metabolites. The current study was intended to resolve the acute influence of intracerebroventricularly injected nesfatin-1 on the levels of some blood electrolytes and metabolites in rats.
The experiments were conducted on Sprague Dawley male rats. Nesfatin-1 (200 pmol) or saline (5 μL) was given the rats intracerebroventricularly. Central nesfatin-1 treatment caused increases in the concentrations of blood glucose, lactate, hematocrit, and hemoglobin without changing the blood pH, creatine, Na, K, Ca, Cl, and HCO3 levels.
In conclusion, our findings show that the central nesfatin-1 could affect the concentrations of blood glucose, lactate, hematocrit, and hemoglobin without altering the blood electrolytes. This could be interpreted as the secondary effect of nesfatin-1 as a consequence of centrally injected nesfatin-1-evoked activation of sympathetic nerves.

Keywords

Nesfatin-1, Intracerebroventricular, Blood electrolytes, Blood metabolites, Hematocrit, Hemoglobin

References

• Schalla MA, Stengel A. Current Understanding of the Role of Nesfatin-1. J Endocr. Soc. 2018;2:1188-1206.
• Oh-I S, Shimizu H, Satoh T, et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature. 2006;443:709–712
• Yilmaz MS, Altinbas B, Guvenc G, et al. The role of centrally injected nesfatin-1 on cardiovascular regulation in normotensive and hypotensive rats. Auton. Neurosci. 2015;193:63-68.
• Aydin B, Guvenc G, Altinbas B, et al. Modulation of nesfatin-1-induced cardiovascular effects by the central cholinergic system. Neuropeptides 2018;70:9-15.
• Ciftci K, Guvenc G, Bas A, et al. Effect of central and peripheral ınjected nesfatin-1 on electrocardiography in rats. J. Res. Vet. Med. 2019;38:10-17.
• Ciftci K, Guvenc G, Kasikci E, et al. Centrally and peripherally injected nesfatin-1-evoked respiratory responses. Respir. Physiol. Neurobiol. 2019;267:6-11.
• Guvenc G, Altinbas B, Kasikci E, et al. Contingent role of phoenixin and nesfatin-1 on secretions of the male reproductive hormones. Andrologia. 2019;51:e13410.
• Stengel A, Goebel M, Yakubov I, et al. Identification and characterization of nesfatin-1 immunoreactivity in endocrine cell types of the rat gastric oxyntic mucosa. Endocrinology. 2009;150:232–238.
• Ramanjaneya M, Chen J, Brown JE, et al. Identification of nesfatin-1 in human and murine adipose tissue: a novel depot-specific adipokine with increased levels in obesity. Endocrinology. 2010;151:3169–3180.
• Gonzalez R, Tiwari A, Unniappan S. Pancreatic beta cells colocalize insulin and pronesfatin immunoreactivity in rodents. Biochem. Biophys. Res. Commun. 2009;381:643–648.
• Garcia-Galiano D, Pineda R, Ilhan T, et al. Cellular distribution, regulated expression, and functional role of the anorexigenic peptide, NUCB2/nesfatin-1 in the testis. Endocrinology. 2012;153:1959–1971.
• Gonzalez R, Shepperd E, Thiruppugazh V, et al. Nesfatin-1 regulates the hypothalamo-pituitary-ovarian axis of fish. Biol. Reprod. 2012;87:84.
• Kim J, Chung Y, Kim H, et al. The tissue distribution of nesfatin-1/NUCB2 in mouse. Dev. Reprod. 2014;18:301–309.
• Feijoo-Bandin S, Rodriguez-Penas D, Garcia-Rua V, et al. Nesfatin-1 in human and murine cardiomyocytes: synthesis, secretion, and mobilization of GLUT-4. Endocrinology. 2013;154:4757–4767.
• Atsuchi K, Asakawa A, Ushikai M, et al. Centrally administered nesfatin-1 inhibits feeding behaviour and gastroduodenal motility in mice. Neuroreport. 2010;21:1008–1011.
• Stengel A, Goebel M, Wang L, et al. Central nesfatin-1 reduces dark-phase food intake and gastric emptying in rats: differential role of corticotropin-releasing factor 2 receptor. Endocrinology. 2009;150:4911–4919.
• Yosten GL, Redlinger L, Samson WK. Evidence for a role of endogenous nesfatin-1 in the control of water drinking. J Neuroendocrinol. 2012;24:1078–1084.
• Konczol K, Pinter O, Ferenczi S, et al. Nesfatin-1 exerts long-term effect on food intake and body temperature. Int. J. Obes. (Lond.). 2012;36:1514–1521.
• Dore R, Levata L, Gachkar S, et al. The thermogenic effect of nesfatin-1 requires recruitment of the melanocortin system. J. Endocrinol. 2017;235:111–122.
• Paxinos G, Watson C. The rat brain in stereotaxic coordinates fourth edition. Academic Press, New York. 2005.
• Goebel-Stengel M, Wang L. Central and peripheral expression and distribution of NUCB2/nesfatin-1. Curr. Pharm. Des. 2013;19:6935-6940.
• Pan W, Hsuchou H, Kastin AJ. Nesfatin-1 crosses the blood–brain barrier without saturation. Peptides 2007;28:2223-2228.
• Kohno D, Nakata M, Maejima Y, et al. Nesfatin-1 neurons in paraventricular and supraoptic nuclei of the rat hypothalamus coexpress oxytocin and vasopressin and are activated by refeeding. Endocrinology. 2008;149:1295–1301.
• Schalla MA, Unniappan S, Lambrecht NWG, et al. NUCB2/nesfatin-1 - Inhibitory effects on food intake, body weight and metabolism. Peptides. 2020;27:170308.
• Yang M, Zhang Z, Wang C, et al. Nesfatin-1 action in the brain increases insulin sensitivity through Akt/AMPK/TORC2 pathway in diet-induced insulin resistance. Diabetes. 2012;61:1959-1968.
• Osaki A, Shimizu H. Peripheral administration of nesfatin-1 increases blood pressure in mice. Hypertens. Res. 2014;37:185-186.
• Tanida M, Gotoh H, Yamamoto N, et al., Hypothalamic nesfatin-1 stimulates sympathetic nerve activity via hypothalamic ERK signaling. Diabetes. 2015;64: 3725-3736.
• Yosten G., Samson WK. Nesfatin-1 exerts cardiovascular actions in brain: possible interaction with the central melanocortin system. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2009;297:R330-336.
• Carnagarin R, Matthews VB, Herat LY, Ho JK, Schlaich MP. Autonomic regulation of glucose homeostasis: a specific role for sympathetic nervous system activation. Curr. Diab. Rep. 2018;18:107.
• Frey GC, McCubbin JA, Dunn JM, et al. Plasma catecholamine and lactate relationship during graded exercise in men with spinal cord injury. Med. Sci. Sports Exerc. 1997;29:451-456.
• Stewart IB, McKenzie DC. The human spleen during physiological stress. Sports Med. 2002;32:361-369.