Genetic variability of FABP4 c.328 G>A (rs110652478) polymorphism and its association with slaughter weight and carcass traits in Aberdeen Angus and Hereford bulls imported into Turkey

Year 2021, , 10 - 18, 19.08.2021

Sena Ardıclı , Hakan Üstüner , Öznur Arslan

https://doi.org/10.30782/jrvm.895372

Cited By: 1

Abstract

The study aimed to determine the genotypic distribution of the c.328 G>A (rs110652478) polymorphism in the bovine fatty acid-binding protein 4 (FABP4) gene and to determine its effect on slaughter weight in Aberdeen Angus and Hereford bulls imported into Turkey. A total of randomly selected 95 bulls were used in this study. Bulls were housed for fattening in semi-open pens for approximately nine months with the same management procedures. Genotyping of the FABP4 c.328 G>A (rs110652478) polymorphism, located in exon 3, was performed by the PCR-RFLP method. Allele and genotype frequencies were calculated by the standard procedures and the Hardy-Weinberg equilibrium was tested by comparing expected and observed genotype frequencies using chi-square and Fisher’s exact tests. Indices of genetic diversity were calculated based on the allelic frequencies. The least-squares method as applied in a GLM procedure was used to test the association between FABP4 c.328 G>A and phenotypic traits including slaughter weight, hot and cold carcass weight, chilling loss, dressing percentage, and carcass pH. Results revealed that the most frequent genotype was GG (49.18% and 52.94% for Angus and Herefords, respectively) and the minor allele frequency (A allele) was 0.32 in the total population. The genotype distribution was in agreement with Hardy Weinberg equilibrium (P>0.05). The moderately low levels of genetic variability observed in this study may be caused as a result of eventual inbreeding. PIC levels suggest that FABP4 c.328 G>A can be a mildly informative genetic marker for the tested population. Association analysis indicated that the effect of the FABP4 on slaughter weight was statistically significant (P<0.05) in the total population. However, this association was not substantiated in breed-specific analyzes. There was no significant effect of the marker on any remaining phenotypic traits. Consequently, it is important to emphasize that analysis of FABP4 c.328 G>A may provide valuable, but limited, information on imported beef cattle raised in Turkey conditions.

Keywords

Beef cattle, FABP4, single nucleotide polymorphism, marker-assisted selection

References

• 1. Goddard M, Hayes B. Genomic selection. Journal of Animal breeding and Genetics 2007;124:323-330.
• 2. Ardicli S, Samli H, Alpay F, et al. Association of single nucleotide polymorphisms in the FABP4 gene with carcass characteristics and meat quality in Holstein bulls. Annals of Animal Science 2017;17:117-130.
• 3. Switonski M. Molecular genetics in beef cattle breeding–a review. Animal Science Papers and Reports 2002;20:7-18.
• 4. Casas E, Shackelford S, Keele J, et al. Detection of quantitative trait loci for growth and carcass composition in cattle. Journal of Animal Science 2003;81:2976-2983.
• 5. Michal J, Zhang Z, Gaskins C, et al. The bovine fatty acid binding protein 4 gene is significantly associated with marbling and subcutaneous fat depth in Wagyu x Limousin F2 crosses. Animal Genetics 2006;37:400-402.
• 6. Goszczynski DE, Papaleo-Mazzucco J, Ripoli MV, et al. Genetic variation in FABP4 and evaluation of its effects on beef cattle fat content. Animal biotechnology 2017;28:211-219.
• 7. Fortes MR, Curi RA, Chardulo LAL, et al. Bovine gene polymorphisms related to fat deposition and meat tenderness. Genetics and Molecular Biology 2009;32:75-82.
• 8. Shin S-C, Heo J-P, Chung E-R. Genetic variants of the FABP4 gene are associated with marbling scores and meat quality grades in Hanwoo (Korean cattle). Molecular biology reports 2012;39:5323-5330.
• 9. Ardicli S, Samli H, Vatansever B, et al. Comprehensive assessment of candidate genes associated with fattening performance in Holstein–Friesian bulls. Archives Animal Breeding 2019;62:9-32.
• 10. Maharani D, Jung Y, Jung W, et al. Association of five candidate genes with fatty acid composition in Korean cattle. Molecular biology reports 2012;39:6113-6121.
• 11. Cho S, Park T-S, Yoon D-H, et al. Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle. BMB reports 2008;41:29-34.
• 12. Lee SH, Van Der Werf J, Lee S, et al. Genetic polymorphisms of the bovine fatty acid binding protein 4 gene are significantly associated with marbling and carcass weight in Hanwoo (Korean Cattle). Animal genetics 2010;41:442-444.
• 13. Sambrook J, Russell DW. Isolation of High-molecular-weight DNA from Mammalian Cells Using Formamide. Cold Spring Harbor Protocols 2006;2006:pdb. prot3225.
• 14. Falconer DS, Mackay TFC. Introduction to quantitative genetics, Pearson Education Ltd, Harlow, England; 1996.
• 15. Botstein D, White RL, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American journal of human genetics 1980;32:314.
• 16. Nei M, Roychoudhury A. Sampling variances of heterozygosity and genetic distance. Genetics 1974;76:379-390.
• 17. Crow JF, Kimura M. Properties of a finite population. In: An introduction to population genetics theory. The Blackburn Press, Caldwell, New Jersey: 319-365; 1970.
• 18. Trakovická A, Moravčíková N, Kasarda R. Genetic polymorphisms of leptin and leptin receptor genes in relation with production and reproduction traits in cattle. Acta Biochimica Polonica 2013;60.
• 19. Ardicli S, Ustüner H, Arslan Ö, et al. Variability of CAPN1 g. 5709 C> G and MYF5 g. 1911 A> G Polymorphisms in Beef Cattle Imported from Brazil to Turkey. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi 2019;59:72-78.
• 20. Hulsegge I, Schoon M, Windig J, et al. Development of a genetic tool for determining breed purity of cattle. Livestock Science 2019;223:60-67.
• 21. Lee S-H, Cho Y-M, Kim H-C, et al. Association of microsatellite marker in FABP4 gene with marbling score and live weight in Hanwoo. Journal of Animal Science and Technology 2010;52:475-480.
• 22. Mizoshita K, Watanabe T, Hayashi H, et al. Quantitative trait loci analysis for growth and carcass traits in a half-sib family of purebred Japanese Black (Wagyu) cattle. Journal of Animal Science 2004;82:3415-3420.
• 23. Ayres D, Souza F, Mercadante M, et al. Evaluation of TFAM and FABP4 gene polymorphisms in three lines of Nellore cattle selected for growth. Genetics and Molecular Research 2010:2050-2059.
• 24. Ardicli S, Dincel D, Samli H, et al. Effects of polymorphisms at LEP, CAST, CAPN1, GHR, FABP4 and DGAT1 genes on fattening performance and carcass traits in Simmental bulls. Archives Animal Breeding 2017;60:61-70.
• 25. Cinar MU, Akyuz B, Kiyici JM, et al. Effects of GH-AluI and MYF5-TaqI polymorphisms on weaning weight and body measurements in Holstein young bulls. Kafkas Universitesi Veteriner Fakültesi Dergisi 2018;24:873-880.
• 26. Avilés C, Polvillo O, Peña F, et al. Associations between DGAT1, FABP4, LEP, RORC, and SCD1 gene polymorphisms and fat deposition in Spanish commercial beef. Journal of Animal Science 2013;91:4571-4577.
• 27. Barendse W, Bunch R, Thomas M, et al. A splice site single nucleotide polymorphism of the fatty acid binding protein 4 gene appears to be associated with intramuscular fat deposition in longissimus muscle in Australian cattle. Animal Genetics 2009;40:770-773.
• 28. Bartoň L, Bureš D, Kott T, et al. Associations of polymorphisms in bovine DGAT1, FABP4, FASN, and PPARGC1A genes with intramuscular fat content and the fatty acid composition of muscle and subcutaneous fat in Fleckvieh bulls. Meat Science 2016;114:18-23.
• 29. Thaller G, Kühn C, Winter A, et al. DGAT1, a new positional and functional candidate gene for intramuscular fat deposition in cattle. Animal Genetics 2003;34:354-357.
• 30. Meuwissen T, Hayes B, Goddard M. Genomic selection: A paradigm shift in animal breeding. Animal Frontiers 2016;6:6-14.