Detection of Shiga-Toxin Producing E.coli (STEC), Enteropathogenic E.coli (EPEC) And Enterotoxigenic E.coli (ETEC) From Animals By M-PCR

Year 2019, Volume: 38 Issue: 2, 88 - 94, 18.12.2019

Esra Büyükcangaz Mohammed Khider Ayşin Şen Murat Cengiz , Songül Sonal

https://doi.org/10.30782/jrvm.624890

Cited By: 1

Abstract

This research investigated the presence of virulence
genes encoding F41, K99, eae, Stx1, Stx2 and STa and the antimicrobial resistance of
animal Escherichia coli (E. coli) isolates. Clinical isolates (n:233) were
evaluated from faecal samples of cattle, sheep, goats, horses, cats and dogs collected
between the years of 2010 to 2015 from Turkey.
Enterohaemorrahic E.coli (EHEC) O157:H7
was detected by using cefixime tellurite sorbitol MacConkey agar (CT-SMAC) and
Wellcollex E. coli (Remel®). The Kirby-Bauer disc diffusion test was performed to detect the resistance pattern
of the isolates to ampicillin, Amoxycillin/clavulanic acid, enrofloxacin,
ceftiofur, trimethoprim/sulfamethoxazole and tetracycline. The results showed
that 40% of the ruminant isolates were identified as Shiga-toxin producing Escherichia coli (STEC).  Enterotoxigenic E. coli (ETEC) was detected
in samples from cattle (0.9%) and sheep (12%). Enteropathogenic E.coli (EPEC) was detected in samples
from cattle (0.9%) and dogs (11.4%). EHEC O157:H7 was not detected any
of the isolates. Among all E. coli isolates that carried at least one
virulence gene, 8 (19%) were resistant to more than three antimicrobials, 7 (16.7%)
were resistant to at least one antimicrobial and 27 (64.3%) were susceptible to
all antimicrobials.

Keywords

Antimicrobial susceptibility, E. coli, Pcr, EHEC, EPEC, ETEC, STEC

Supporting Institution

Uludağ Üniversitesi

Project Number

KUAP (V) 2014/43

Thanks

This research was supported by Uludag University Scientific Research Projects KUAP (V) 2014/43 entitled ‘Determination of some virulence genes of Escherichia coli isolates from animals by multiplex PCR’ and a part of the doctorate thesis (2017) of Mohammed Khider entitled’ PCR Detection of some virulence genes in Escherichia coli isolates obtained from animal sources’.

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