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First isolation and characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) strain from respiratory tract of cattle in Turkey

Year 2022, Volume: 41 Issue: 2, 80 - 87, 31.12.2022

Gizem Aytoğu , Kadir Yeşilbağ , Eda Baldan Toker , Berfin Kadiroğlu , Özer Ateş , Pelin Tuncer-göktuna

https://doi.org/10.30782/jrvm.1082913

Abstract

Restriksiyon Parça Uzunluk Polimorfizmi (RFLP) tekniği kullanılarak tüm genomun analizinde Bovine Herpesviruses-1 (BoHV-1) suşlarını BoHV-1.1, 1.2a, 1.2b olmak üzere üç alt tipte sınıflandırılmaktadır. Bu alt tipler klinik bulgular göre de değerlendirilmektedir ancak bu yönde henüz net bir ilişkilendirme yapılmamıştır. Çoğunlukla BoHV-1.2b, genital hastalık bulgularıyla ilişkilendirilmektedir. Bu çalışmada solunum yolu hastalığından alınan nazal sürüntü örneğinde BoHV-1 izolatı elde edilmiştir. Bu çalışmada ayrıca, genomun UL44 (Glikoprotein C) bölgesini hedefleyen filogenetik analiz ve UL39 - US3 bölgelerini hedefleyen, yeni geliştirilen bir multipleks PCR analizini takiben Hind III enzimi kullanılarak uygulanan RFLP ile alt tiplendirme gerçekleştirilmiştir. Aynı zamanda izolasyon başarısı iki farklı devamlı hücre hattında karşılaştırılmıştır. SFT-R hücre hattı, BoHV-1 saha örneklerinin izolasyonunda MDBK hücre hattına nazaran daha duyarlı bulunmuştur. Elde edilen izolat (ID:8640), BoHV-1.2b olarak sınıflandırılırken, Cooper suşundan serolojik olarak ayırt edilemediği değerlendirildi. Mevcut çalışma, BoHV-1.2b'nin Türkiye'deki ilk izolasyonunu bildirmesinin yanı sıra klinik solunum yolu hastalığından nadir tespit edilen BoHV-1.2b izolasyonunu bildirmektedir. Sonuçlar ayrıca kolay ve hızlı alt tipleme için PCR tabanlı RFLP analizinin etkinliğini gösteren veriler içermektedir. Ancak BoHV-1 saha izolatlarının genetik çeşitliliğine bağlı olarak bu teknik üzerine daha fazla araştırma yapılmasına ihtiyaç duyulmaktadır.

Keywords

BoHV-1 subtyping molecular characterization restriction endonuclease analysis virus isolation

Supporting Institution

Bursa Uludağ University Research Fund (BUU-BAP); Turkish Scientific and Technological Research Council (TUBİTAK)

Project Number

OUAP(V)-2020/7; 119 O 571

References

  • Raaperi K, Orro T, Viltrop A. Epidemiology and control of bovine herpesvirus 1 infection in Europe. Vet J. 2014;201(3):249-256. doi:10.1016/j.tvjl.2014.05.040
  • Nandi S, Kumar M, Manohar M, Chauhan RS. Bovine herpes virus infections in cattle. Anim Health Res Rev. 2009;10(1):85-98. doi:10.1017/S1466252309990028
  • Sayers RG. Associations between exposure to bovine herpesvirus 1 (BoHV-1) and milk production, reproductive performance, and mortality in Irish dairy herds. J Dairy Sci. 2017;100(2):1340-1352. doi:10.3168/jds.2016-11113
  • Liang X, Chow B, Raggo C, Babiuk LA. Bovine herpesvirus 1 UL49.5 homolog gene encodes a novel viral envelope protein that forms a disulfide-linked complex with a second virion structural protein. J Virol. 1996;70(3).
  • Fulton RW, d’Offay JM, Eberle R, et al. Bovine herpesvirus-1: Evaluation of genetic diversity of subtypes derived from field strains of varied clinical syndromes and their relationship to vaccine strains. Vaccine. 2015;33(4):549-558. doi:10.1016/j.vaccine.2014.11.033
  • Miller JM, Whetstone CA, der Maaten MJ. Abortifacient property of bovine herpesvirus type 1 isolates that represent three subtypes determined by restriction endonuclease analysis of viral DNA. Am J Vet Res. 1991;52(3):458—461. http://europepmc.org/abstract/MED/1852144.
  • Rosado Spilki F, Augusto Esteves P, De Lima M, et al. Comparative pathogenicity of bovine herpesvirus 1 (BHV-1) subtypes 1 (BHV-1.1) and 2a (BHV-1.2a). Pesqui Vet Bras. 2004;24(1):43-49. doi:10.1590/s0100-736x2004000100010
  • Biswas S, Bandyopadhyay S, Dimri U, H. Patra P. Bovine herpesvirus-1 (BHV-1) - a re-emerging concern in livestock: A revisit to its biology, epidemiology, diagnosis, and prophylaxis. Vet Q. 2013;33(2):68-81. doi:10.1080/01652176.2013.799301
  • Gregersen JP, Pauli G, Ludwig H. Bovine Herpesvirus 1: Differentiation of IBR- and IPV-viruses and identification and functional role of their major immunogenic components. Arch Virol. 1985;84(1-2):91-103. doi:10.1007/BF01310556
  • d’Offay JM, Eberle R, Fulton RW, Kirkland PD. Complete genomic sequence and comparative analysis of four genital and respiratory isolates of bovine herpesvirus subtype 1.2b (BoHV-1.2b), including the prototype virus strain K22. Arch Virol. 2016;161(11):3269-3274. doi:10.1007/s00705-016-3026-1
  • Esteves PA, Dellagostin OA, Pinto LS, et al. Phylogenetic comparison of the carboxy-terminal region of glycoprotein C (gC) of bovine herpesviruses (BoHV) 1.1, 1.2 and 5 from South America (SA). Virus Res. 2008;131(1):16-22. doi:10.1016/j.virusres.2007.08.004
  • Vilc̆ek Š. Detection of the bovine herpesvirus-1 (BHV-1) genome by PCR. J Virol Methods. 1993;41(2):245-247. doi:10.1016/0166-0934(93)90132-B
  • Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95-98. doi:citeulike-article-id:691774
  • Maidana SS, Miño S, Apostolo RM, De Stefano GA, Romera SA. A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates. J Vet Diagnostic Investig. 2020;32(1):112-117. doi:10.1177/1040638719898692
  • Muylkens B, Thiry J, Kirten P, Schynts F, Thiry E. Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis. Vet Res. 2007;38(2):181-209. doi:10.1051/vetres:2006059
  • Kendrick J, Gillespie J, Mcentee K. Infectious Pustular Vulvovaginitis of Cattle. Cornell Vet. 1958;48(4):466-495. https://babel.hathitrust.org/cgi/pt?id=uc1.b4179384&view=1up&seq=466. Accessed June 16, 2020.
  • Ludwig H, Gregersen JP. Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis: BHV-1 infections. Rev sec tech Off int Epiz. 1986;5(4):869-878.
  • Whetstone CA, Miller JM. Two different strains of an alphaherpesvirus can establish latency in the same tissue of the host animal: evidence from bovine herpesvirus 1. Arch Virol. 1989;107(1-2):27-34. doi:10.1007/BF01313875
  • Fulton RW, d’Offay JM, Dubovi EJ, Eberle R. Bovine herpesvirus-1: Genetic diversity of field strains from cattle with respiratory disease, genital, fetal disease and systemic neonatal disease and their relationship to vaccine strains. Virus Res. 2016;223:115-121. doi:10.1016/j.virusres.2016.06.017
  • Edwards S, White H, Nixon P. A study of the predominant genotypes of bovid herpesvirus 1 found in the U.K. Vet Microbiol. 1990;22(2-3):213-223. doi:10.1016/0378-1135(90)90108-8
  • Bilge Dagalp S, Touraj, Farzani A, Dogan F, Alkan F, Ozkul A. Molecular and antigenic characterization of bovine herpesvirus type 1 (BoHV-1) strains from cattle with diverse clinical cases in Turkey. Trop Anim Heal Prod (. 2020;52:555–564. doi:10.1007/s11250-019-02042-6
  • Traesel CK, Silva MSE, Spilki FR, Weiblen R, Flores EF. Nucleotide sequencing and phylogenetic analysis of the 3’ region of glycoprotein C gene of South American bovine herpesviruses 1 and 5. Res Vet Sci. 2013;94(1):178-185. doi:10.1016/j.rvsc.2012.07.032
  • Zhou Y, Li X, Ren Y, et al. Phylogenetic analysis and characterization of bovine herpesvirus-1 in cattle of China, 2016–2019. Infect Genet Evol. June 2020:104416. doi:10.1016/j.meegid.2020.104416
  • D’Arce RCF, Almeida RS, Silva TC, et al. Restriction endonuclease and monoclonal antibody analysis of Brazilian isolates of bovine herpesviruses types 1 and 5. Vet Microbiol. 2002;88(4):315-324. doi:10.1016/S0378-1135(02)00126-8
  • Oliveira MT, Campos FS, Dias MM, et al. Detection of bovine herpesvirus 1 and 5 in semen from Brazilian bulls. Theriogenology. 2011;75(6):1139-1145. doi:10.1016/j.theriogenology.2010.11.025
  • Julian RS, Jaime J, Ramirez G, Vera V. Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Virol Sin. 2012;27(1):26-37. doi:10.1007/s12250-012-3221-5
  • Maidana SS, Morano CD, Cianfrini D, et al. Multiplex PCR followed by restriction length polymorphism analysis for the subtyping of bovine herpesvirus 5 isolates. BMC Vet Res. 2013;9:111. doi:10.1186/1746-6148-9-111
  • Maidana SS, Ladelfa MF, Pérez SE, et al. Characterization of BoHV-5 field strains circulation and report of transient specific subtype of bovine herpesvirus 5 in Argentina. BMC Vet Res. 2011;7:8. doi:10.1186/1746-6148-7-8
  • Ros C, Belák S. Studies of Genetic Relationships Between Bovine, Caprine, Cervine, and Rangiferine Alphaherpesviruses and Improved Molecular Methods for Virus Detection and Identification - PubMed. J Clin Microbiol. 1999;37(5):1247-1253. https://pubmed.ncbi.nlm.nih.gov/10203465/. Accessed June 25, 2020.
  • Aslan ME, Azkur AK, Gazyağcı S. Epidemiology and genetic characterization of BVDV, BHV-1, BHV-4, BHV-5 and Brucella spp. infections in cattle in Turkey. J Vet Med Sci. 2015;77(11):1371-1377. doi:10.1292/jvms.14-0657
  • Albayrak H, Tamer C, Ozan E, et al. Molecular identification and phylogeny of bovine herpesvirus-1 (BoHV-1) from cattle associated with respiratory disorders and death in Turkey. Med Weter. 2020;76(06):6393-2020. doi:10.21521/mw.6393
  • Varela APM, Holz CL, Cibulski SP, et al. Neutralizing antibodies to bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) and its subtypes. Vet Microbiol. 2010;142(3-4):254-260. doi:10.1016/j.vetmic.2009.10.016
  • Samrath D, Shakya S, Rawat N, Gilhare VR, Singh F. Isolation and adaptation of bovine herpes virus Type 1 in embryonated chicken eggs and in Madin-Darby bovine kidney cell line. Vet World. 2016;9(2):222-225. doi:10.14202/vetworld.2016.222-225
  • Matsuura K, Inoshima Y, Kameyama KI, Murakami K. Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species. Vitr Cell Dev Biol - Anim. 2011;47(7):459-463. doi:10.1007/s11626-011-9434-3

Year 2022, Volume: 41 Issue: 2, 80 - 87, 31.12.2022

Gizem Aytoğu , Kadir Yeşilbağ , Eda Baldan Toker , Berfin Kadiroğlu , Özer Ateş , Pelin Tuncer-göktuna

https://doi.org/10.30782/jrvm.1082913

Abstract

Project Number

OUAP(V)-2020/7; 119 O 571

References

  • Raaperi K, Orro T, Viltrop A. Epidemiology and control of bovine herpesvirus 1 infection in Europe. Vet J. 2014;201(3):249-256. doi:10.1016/j.tvjl.2014.05.040
  • Nandi S, Kumar M, Manohar M, Chauhan RS. Bovine herpes virus infections in cattle. Anim Health Res Rev. 2009;10(1):85-98. doi:10.1017/S1466252309990028
  • Sayers RG. Associations between exposure to bovine herpesvirus 1 (BoHV-1) and milk production, reproductive performance, and mortality in Irish dairy herds. J Dairy Sci. 2017;100(2):1340-1352. doi:10.3168/jds.2016-11113
  • Liang X, Chow B, Raggo C, Babiuk LA. Bovine herpesvirus 1 UL49.5 homolog gene encodes a novel viral envelope protein that forms a disulfide-linked complex with a second virion structural protein. J Virol. 1996;70(3).
  • Fulton RW, d’Offay JM, Eberle R, et al. Bovine herpesvirus-1: Evaluation of genetic diversity of subtypes derived from field strains of varied clinical syndromes and their relationship to vaccine strains. Vaccine. 2015;33(4):549-558. doi:10.1016/j.vaccine.2014.11.033
  • Miller JM, Whetstone CA, der Maaten MJ. Abortifacient property of bovine herpesvirus type 1 isolates that represent three subtypes determined by restriction endonuclease analysis of viral DNA. Am J Vet Res. 1991;52(3):458—461. http://europepmc.org/abstract/MED/1852144.
  • Rosado Spilki F, Augusto Esteves P, De Lima M, et al. Comparative pathogenicity of bovine herpesvirus 1 (BHV-1) subtypes 1 (BHV-1.1) and 2a (BHV-1.2a). Pesqui Vet Bras. 2004;24(1):43-49. doi:10.1590/s0100-736x2004000100010
  • Biswas S, Bandyopadhyay S, Dimri U, H. Patra P. Bovine herpesvirus-1 (BHV-1) - a re-emerging concern in livestock: A revisit to its biology, epidemiology, diagnosis, and prophylaxis. Vet Q. 2013;33(2):68-81. doi:10.1080/01652176.2013.799301
  • Gregersen JP, Pauli G, Ludwig H. Bovine Herpesvirus 1: Differentiation of IBR- and IPV-viruses and identification and functional role of their major immunogenic components. Arch Virol. 1985;84(1-2):91-103. doi:10.1007/BF01310556
  • d’Offay JM, Eberle R, Fulton RW, Kirkland PD. Complete genomic sequence and comparative analysis of four genital and respiratory isolates of bovine herpesvirus subtype 1.2b (BoHV-1.2b), including the prototype virus strain K22. Arch Virol. 2016;161(11):3269-3274. doi:10.1007/s00705-016-3026-1
  • Esteves PA, Dellagostin OA, Pinto LS, et al. Phylogenetic comparison of the carboxy-terminal region of glycoprotein C (gC) of bovine herpesviruses (BoHV) 1.1, 1.2 and 5 from South America (SA). Virus Res. 2008;131(1):16-22. doi:10.1016/j.virusres.2007.08.004
  • Vilc̆ek Š. Detection of the bovine herpesvirus-1 (BHV-1) genome by PCR. J Virol Methods. 1993;41(2):245-247. doi:10.1016/0166-0934(93)90132-B
  • Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95-98. doi:citeulike-article-id:691774
  • Maidana SS, Miño S, Apostolo RM, De Stefano GA, Romera SA. A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates. J Vet Diagnostic Investig. 2020;32(1):112-117. doi:10.1177/1040638719898692
  • Muylkens B, Thiry J, Kirten P, Schynts F, Thiry E. Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis. Vet Res. 2007;38(2):181-209. doi:10.1051/vetres:2006059
  • Kendrick J, Gillespie J, Mcentee K. Infectious Pustular Vulvovaginitis of Cattle. Cornell Vet. 1958;48(4):466-495. https://babel.hathitrust.org/cgi/pt?id=uc1.b4179384&view=1up&seq=466. Accessed June 16, 2020.
  • Ludwig H, Gregersen JP. Infectious bovine rhinotracheitis/infectious pustular vulvovaginitis: BHV-1 infections. Rev sec tech Off int Epiz. 1986;5(4):869-878.
  • Whetstone CA, Miller JM. Two different strains of an alphaherpesvirus can establish latency in the same tissue of the host animal: evidence from bovine herpesvirus 1. Arch Virol. 1989;107(1-2):27-34. doi:10.1007/BF01313875
  • Fulton RW, d’Offay JM, Dubovi EJ, Eberle R. Bovine herpesvirus-1: Genetic diversity of field strains from cattle with respiratory disease, genital, fetal disease and systemic neonatal disease and their relationship to vaccine strains. Virus Res. 2016;223:115-121. doi:10.1016/j.virusres.2016.06.017
  • Edwards S, White H, Nixon P. A study of the predominant genotypes of bovid herpesvirus 1 found in the U.K. Vet Microbiol. 1990;22(2-3):213-223. doi:10.1016/0378-1135(90)90108-8
  • Bilge Dagalp S, Touraj, Farzani A, Dogan F, Alkan F, Ozkul A. Molecular and antigenic characterization of bovine herpesvirus type 1 (BoHV-1) strains from cattle with diverse clinical cases in Turkey. Trop Anim Heal Prod (. 2020;52:555–564. doi:10.1007/s11250-019-02042-6
  • Traesel CK, Silva MSE, Spilki FR, Weiblen R, Flores EF. Nucleotide sequencing and phylogenetic analysis of the 3’ region of glycoprotein C gene of South American bovine herpesviruses 1 and 5. Res Vet Sci. 2013;94(1):178-185. doi:10.1016/j.rvsc.2012.07.032
  • Zhou Y, Li X, Ren Y, et al. Phylogenetic analysis and characterization of bovine herpesvirus-1 in cattle of China, 2016–2019. Infect Genet Evol. June 2020:104416. doi:10.1016/j.meegid.2020.104416
  • D’Arce RCF, Almeida RS, Silva TC, et al. Restriction endonuclease and monoclonal antibody analysis of Brazilian isolates of bovine herpesviruses types 1 and 5. Vet Microbiol. 2002;88(4):315-324. doi:10.1016/S0378-1135(02)00126-8
  • Oliveira MT, Campos FS, Dias MM, et al. Detection of bovine herpesvirus 1 and 5 in semen from Brazilian bulls. Theriogenology. 2011;75(6):1139-1145. doi:10.1016/j.theriogenology.2010.11.025
  • Julian RS, Jaime J, Ramirez G, Vera V. Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Virol Sin. 2012;27(1):26-37. doi:10.1007/s12250-012-3221-5
  • Maidana SS, Morano CD, Cianfrini D, et al. Multiplex PCR followed by restriction length polymorphism analysis for the subtyping of bovine herpesvirus 5 isolates. BMC Vet Res. 2013;9:111. doi:10.1186/1746-6148-9-111
  • Maidana SS, Ladelfa MF, Pérez SE, et al. Characterization of BoHV-5 field strains circulation and report of transient specific subtype of bovine herpesvirus 5 in Argentina. BMC Vet Res. 2011;7:8. doi:10.1186/1746-6148-7-8
  • Ros C, Belák S. Studies of Genetic Relationships Between Bovine, Caprine, Cervine, and Rangiferine Alphaherpesviruses and Improved Molecular Methods for Virus Detection and Identification - PubMed. J Clin Microbiol. 1999;37(5):1247-1253. https://pubmed.ncbi.nlm.nih.gov/10203465/. Accessed June 25, 2020.
  • Aslan ME, Azkur AK, Gazyağcı S. Epidemiology and genetic characterization of BVDV, BHV-1, BHV-4, BHV-5 and Brucella spp. infections in cattle in Turkey. J Vet Med Sci. 2015;77(11):1371-1377. doi:10.1292/jvms.14-0657
  • Albayrak H, Tamer C, Ozan E, et al. Molecular identification and phylogeny of bovine herpesvirus-1 (BoHV-1) from cattle associated with respiratory disorders and death in Turkey. Med Weter. 2020;76(06):6393-2020. doi:10.21521/mw.6393
  • Varela APM, Holz CL, Cibulski SP, et al. Neutralizing antibodies to bovine herpesvirus types 1 (BoHV-1) and 5 (BoHV-5) and its subtypes. Vet Microbiol. 2010;142(3-4):254-260. doi:10.1016/j.vetmic.2009.10.016
  • Samrath D, Shakya S, Rawat N, Gilhare VR, Singh F. Isolation and adaptation of bovine herpes virus Type 1 in embryonated chicken eggs and in Madin-Darby bovine kidney cell line. Vet World. 2016;9(2):222-225. doi:10.14202/vetworld.2016.222-225
  • Matsuura K, Inoshima Y, Kameyama KI, Murakami K. Establishment of a novel ovine kidney cell line for isolation and propagation of viruses infecting domestic cloven-hoofed animal species. Vitr Cell Dev Biol - Anim. 2011;47(7):459-463. doi:10.1007/s11626-011-9434-3
There are 34 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Research Articles
Authors

Gizem Aytoğu 0000-0002-3411-081X

Kadir Yeşilbağ 0000-0003-1793-6879

Eda Baldan Toker 0000-0003-2468-3945

Berfin Kadiroğlu 0000-0001-5969-6127

Özer Ateş 0000-0001-7676-9033

Pelin Tuncer-göktuna 0000-0002-7468-0155

Project Number OUAP(V)-2020/7; 119 O 571
Publication Date December 31, 2022
Acceptance Date July 25, 2022
Published in Issue Year 2022 Volume: 41 Issue: 2

Cite

APA Aytoğu, G., Yeşilbağ, K., Toker, E. B., Kadiroğlu, B., et al. (2022). First isolation and characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) strain from respiratory tract of cattle in Turkey. Journal of Research in Veterinary Medicine, 41(2), 80-87. https://doi.org/10.30782/jrvm.1082913
AMA Aytoğu G, Yeşilbağ K, Toker EB, Kadiroğlu B, Ateş Ö, Tuncer-göktuna P. First isolation and characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) strain from respiratory tract of cattle in Turkey. J Res Vet Med. December 2022;41(2):80-87. doi:10.30782/jrvm.1082913
Chicago Aytoğu, Gizem, Kadir Yeşilbağ, Eda Baldan Toker, Berfin Kadiroğlu, Özer Ateş, and Pelin Tuncer-göktuna. “First Isolation and Characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) Strain from Respiratory Tract of Cattle in Turkey”. Journal of Research in Veterinary Medicine 41, no. 2 (December 2022): 80-87. https://doi.org/10.30782/jrvm.1082913.
EndNote Aytoğu G, Yeşilbağ K, Toker EB, Kadiroğlu B, Ateş Ö, Tuncer-göktuna P (December 1, 2022) First isolation and characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) strain from respiratory tract of cattle in Turkey. Journal of Research in Veterinary Medicine 41 2 80–87.
IEEE G. Aytoğu, K. Yeşilbağ, E. B. Toker, B. Kadiroğlu, Ö. Ateş, and P. Tuncer-göktuna, “First isolation and characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) strain from respiratory tract of cattle in Turkey”, J Res Vet Med, vol. 41, no. 2, pp. 80–87, 2022, doi: 10.30782/jrvm.1082913.
ISNAD Aytoğu, Gizem et al. “First Isolation and Characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) Strain from Respiratory Tract of Cattle in Turkey”. Journal of Research in Veterinary Medicine 41/2 (December 2022), 80-87. https://doi.org/10.30782/jrvm.1082913.
JAMA Aytoğu G, Yeşilbağ K, Toker EB, Kadiroğlu B, Ateş Ö, Tuncer-göktuna P. First isolation and characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) strain from respiratory tract of cattle in Turkey. J Res Vet Med. 2022;41:80–87.
MLA Aytoğu, Gizem et al. “First Isolation and Characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) Strain from Respiratory Tract of Cattle in Turkey”. Journal of Research in Veterinary Medicine, vol. 41, no. 2, 2022, pp. 80-87, doi:10.30782/jrvm.1082913.
Vancouver Aytoğu G, Yeşilbağ K, Toker EB, Kadiroğlu B, Ateş Ö, Tuncer-göktuna P. First isolation and characterization of Bovine Herpesvirus 1.2b (BoHV-1.2b ) strain from respiratory tract of cattle in Turkey. J Res Vet Med. 2022;41(2):80-7.
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