Comparison of the Efficacy of Enrofloxacin and Lactobacillus Plantarum Cell-Free Supernatant Treatments on Vaginitis in Ewes

Year 2022, Volume: 41 Issue: 2, 105 - 111, 31.12.2022

Barış Güner , İhsan Kısadere , Hakan Tavşanlı , Serpil Kahya Demirbilek , Abdulkadir Keskin

https://doi.org/10.30782/jrvm.1139354

Cited By: 1

Abstract

The aim of the study was to investigate the effect of different intravaginal treatment strategies on the vaginal discharge score, vaginal microbiota, bacterial and Enterobacteriaceae counts in nulliparous Merino ewes. All ewes (n=45) received intravaginal sponges containing 60 mg medroxyprogesterone acetate for 7 days and allocated into three equal groups (n=15). Sponges were injected Lactobacillus plantarum cell-free supernatant (SUPER), enrofloxacin (ENRO), or physiologic saline (CON) prior to sponge insertion. At sponge removal, 500 IU equine chorionic gonadotropin (eCG) were administered in all ewes. For the detection of vaginal microbiota, bacterial and Enterobactericeae counts, samples were collected prior to sponge insertion, at sponge withdrawal, and 48 h later after sponge withdrawal. Vaginal discharge score was not different in ENRO (2.26±0.18) and SUPER (2.20±0.14) compared to CON (2.46±0.16). The time-dependent alteration was significant for the mean bacterial and Enterobacteriaceae count in all groups (P<0.05). Bacterial counts were found to be lower in ENRO (5.50±0.17) than SUPER (6.31±0.19) and CON (6.07±0.15) at sponge removal (P<0.05). In addition, SUPER (3.74±0.21) and ENRO (3.49±0.27) had lower Enterobacteriaceae counts compared to CON (4.78±0.21) at sponge removal (P<0.01). The most frequently isolated bacteria species were Trueperella pyogenes (28.9%) and Escherichia coli (46.7%). In conclusion, treatment with enrofloxacin or cell-free supernatant decreased the Enterobacteriaceae counts in ewes. Comprehensive studies are needed to assess the effectiveness of lactic acid bacteria as an antibiotic-free treatment strategy on vaginitis in ewes that were synchronized with progesterone impregnated intravaginal sponge.

Keywords

Antibiotic, Bacterial count, Lactobacillus plantarum, Sheep, Vaginitis

Supporting Institution

YOKTUR

Project Number

YOKTUR

Thanks

The authors thank to Dr. Mehmet ÖZÜİÇLİ and Mehmet ÇAN for the his help in this study.

References

• 1. Abecia J, Forcada F, González-bulnes A. Hormonal control of reproduction in small ruminants. Anim Reprod Sci. 2012; 130: 173–179.
• 2. Suárez G, Zunino P, Carol H, et al. Changes in the aerobic vaginal bacterial mucous load and assessment of the susceptibility to antibiotics after treatment with intravaginal sponges in anestrous ewes. Small Rumin Res. 2006; 63: 39–43.
• 3. Manes J, Campero C, Hozbor F, et al. Vaginal histological changes after using intravaginal sponges for oestrous synchronization in anoestrous ewes. Reprod Domest Anim. 2015; 50: 270–274.
• 4. Guner B, Saat N. Comparison of pregnancy rates after short-term and long-term synchronization protocol in ewes- Pilot study. Erciyes Üniversitesi Vet Fak Derg. 2021; 13: 69–74.
• 5. Gatti M, Zunino P, Ungerfeld R. Changes in the aerobic vaginal bacterial mucous load after treatment with intravaginal sponges in anoestrous ewes: Effect of medroxiprogesterone acetate and antibiotic treatment use. Reprod Domest Anim. 2011; 46: 205–208.
• 6. Manes J, Ríos G, Andrea M, et al. Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa. Theriogenology. 2016; 85: 856–861.
• 7. Gatti M, Ungerfeld R. Intravaginal sponges to synchronize estrus decrease sexual attractiveness in ewes. Theriogenology. 2012; 78: 1796–1799.
• 8. Ojeda-Hernández F, del Moral-Ventura S, Capataz-Tafur J, et al. Vaginal microbiota in Pelibuey sheep treated with antimicrobials at the removal of intravaginal sponges impregnated with flurogestone acetate. Small Rumin Res. 2019; 170: 116–119.
• 9. Quereda JJ, García-Roselló E, Barba M, et al. Use of probiotics in intravaginal sponges in sheep: A pilot study. Animals. 2020; 10.
• 10. Mohammed K, Nabih A, Darwish G. Efficacy of anti-microbial agents on vaginal microorganisms and reproductive performance of synchronized estrus ewes. Asian Pacific J Reprod. 2017; 6: 121–127.
• 11. Manes J, Fiorentino MA, Martino SS, et al. Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle. Livest Sci. 2018; 208: 55–59.
• 12. Oliveira JK, Martins G, Esteves LV., et al. Changes in the vaginal flora of goats following a short-term protocol of oestrus induction and synchronisation with intravaginal sponges as well as their antimicrobial sensitivity. Small Rumin Res. 2013; 113: 162–166.
• 13. Viñoles C, Paganoni B, Milton JTB, et al. Pregnancy rate and prolificacy after artificial insemination in ewes following synchronisation with prostaglandin, sponges, or sponges with bactericide. Anim Prod Sci. 2011; 51: 565–569.
• 14. Penna B, Libonati H, Director A, et al. Progestin-impregnated intravaginal sponges for estrus induction and synchronization influences on goats vaginal flora and antimicrobial susceptibility. Anim Reprod Sci. 2013; 142: 71–74.
• 15. Romero T, Balado J, Althaus RL, et al. Short communication: Drug residues in goat milk after prophylactic use of antibiotics in intravaginal sponges for estrus synchronization. J Dairy Sci. 2016; 99: 141–145.
• 16. Berruga MI, Rodriguez A, Rubio R, et al. Short communication: Antibiotic residues in milk following the use of intravaginal sponges for estrus synchronization in dairy ewes. J Dairy Sci. 2008; 91: 3917–3921.
• 17. Pino A, Bartolo E, Caggia C, et al. Detection of vaginal lactobacilli as probiotic candidates. Sci Rep. 2019; 9: 1–10.
• 18. Chen CC, Lai CC, Huang HL, et al. Antimicrobial activity of lactobacillus species against carbapenem-resistant enterobacteriaceae. Front Microbiol. 2019; 10: 1–10.
• 19. Poppi LB, Rivaldi JD, Coutinho TS, et al. Effect of Lactobacillus sp. isolates supernatant on Escherichia coli O157: H7 enhances the role of organic acids production as a factor for pathogen control. Pesqui Vet Bras. 2015; 35: 353–359.
• 20. Dinev T, Beev G, Tzanova M, et al. Antimicrobial activity of lactobacillus plantarum against pathogenic and food spoilage microorganisms: A review. Bulg J Vet Med. 2018; 21: 253–268.
• 21. Athanassiadis B, Abbott P V., George N, et al. An in vitro study of the antimicrobial activity of some endodontic medicaments and their bases using an agar well diffusion assay. Aust Dent J. 2009; 54: 141–146.
• 22. Martinez-Ros P, Lozano M, Hernandez F, et al. Intravaginal device-type and treatment-length for ovine estrus synchronization modify vaginal mucus and microbiota and affect fertility. Animals. 2018; 8: 1–8.
• 23. Swelum AAA, Alowaimer AN, Abouheif MA. Use of fluorogestone acetate sponges or controlled internal drug release for estrus synchronization in ewes: Effects of hormonal profiles and reproductive performance. Theriogenology. 2015; 84: 498–503.
• 24. Ahern CP, Jennings JJ. The bacteriology of vaginal mucus and intravaginal sponges from sheep and the effect of coating sponges with antibacterial agents. Ir Vet J. 1976; 30: 111–117.
• 25. Martins G, Brandão FZ, Figueira L, et al. Prevalence and antimicrobial susceptibility of Staphylococci isolated from the vagina of healthy ewes. Rev Bras Ciência Veterinária. 2009; 16: 37–40.
• 26. Manes J, Fiorentino MA, Kaiser G, et al. Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes. Small Rumin Res. 2010; 94: 201–204.
• 27. Vasconcelos CO de P, Brandão FZ, Martins G, et al. Qualitative and quantitative analysis of bacteria from vaginitis associated with intravaginal implants in ewes following estrus synchronization. Ciência Rural. 2016; 46: 632–636.
• 28. Ungerfeld R, Rubianes E. Short term primings with different progestogen intravaginal devices (MAP, FGA and CIDR) for eCG-estrous induction in anestrus ewes. Small Rumin Res. 2002; 46: 63–66.
• 29. Manes J, Fiorentino MA, Hozbor F, et al. Changes in the aerobic vaginal bacteria load and antimicrobial susceptibility after different oestrous synchronisation treatments in goats. Anim Prod Sci. 2013; 53: 555–559.
• 30. Silva VF, Damasceno TEF, Souza NJD, et al. Microbiota cérvico-vaginal de ovelhas mestiças e sua susceptibilidade aos antibióticos. Pesqui Vet Bras. 2011; 31: 586–590.
• 31. Arrioja-Bretón D, Mani-López E, Palou E, et al. Antimicrobial activity and storage stability of cell-free supernatants from lactic acid bacteria and their applications with fresh beef. Food Control. 2020; 115: 107286.
• 32. Peter S, Gärtner MA, Michel G, et al. Influence of intrauterine administration of lactobacillus buchneri on reproductive performance and pro-inflammatory endometrial mRNA expression of cows with subclinical endometritis. Sci Rep. 2018; 8: 1–13.
• 33. Genís S, Sánchez-Chardi A, Bach À, et al. A combination of lactic acid bacteria regulates Escherichia coli infection and inflammation of the bovine endometrium. J Dairy Sci. 2017; 100: 479–492.