Arı Sütü Takviyesinin Sıçan Akciğerinde Tüketici Egzersiz Kaynaklı Oksidatif Stres Üzerine Etkileri

Year 2021, Volume: 3 Issue: 2, 83 - 88, 31.12.2021

Tülay Özhan Bakır , Murat Bakır

Abstract

Bu çalışmanın amacı, Arı Sütü (AS)’nün, tüketici egzersize bağlı oksidatif hasar üzerindeki etkilerini araştırmaktı. Çalışmayı gerçekleştirmek için sıçanları 4 gruba ayırdık: Kontrol (K), AS tedavisi ile Kontrol (K+AS), kontrol diyeti ile tüketici egzersiz (K+TE) ve AS tedavisi ile tüketici egzersiz (AS+TE). K+AS ve AS+TE gruplarındaki sıçanlar 14 gün boyunca 100 mg/kg AS diyeti ile beslendi. Deneyin 14. Gününde K+TE ve AS+TE gruplarındaki sıçanlar, tüketici yüzme egzersizine tabi tutuldu. Bu çalışmada akciğer oksidatif stres indeksi [malondialdehit (MDA)] ve antioksidan savunma sistemleri [süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSHPx)] incelenmiştir. Lipid profillerini analiz etmek için kan örnekleri toplandı. AS tedavisi, toplam kolesterol (TK), düşük yoğunluklu lipoprotein (LDL) serum seviyelerini önemli ölçüde azalttı ve yüksek yoğunluklu lipoprotein (HDL) seviyesini önemli ölçüde arttırdı (P<0.05). AS takviyesi, akciğerde egzersize bağlı MDA seviyelerindeki artışları önemli ölçüde azalttı (P<0.05). AS, SOD aktivitelerinde egzersize bağlı artışı tersine çevirdi, ancak AS takviyesi CAT ve GSHPx aktivitelerini arttırdı. Sonuç olarak, Arı Sütü takviyesi akciğerdeki MDA seviyelerinin yükselmesini önleyebilir ve tüketici egzersiz sonrası akciğer antioksidan savunma sistemleri üzerinde olumlu bir etkiye sahip olabilir.

Keywords

Arı sütü, oksidatif stres, antioksidan enzimler, tüketici egzersiz

Effects of Royal Jelly Supplement on Exhaustive Exercise-Induced Oxidative Stress in Rat Lung

Abstract

The aim of this study was to investigate the effects of Royal Jelly (RJ) on exhaustive exercise-induced oxidative damage. To perform the study, we divided rats into four groups: control (C), control with RJ treatment (C+RJ), exhaustive exercise with control diet (C+E) and exhaustive exercise with RJ treatment (E+RJ). Rats in C+RJ and RJ+E groups were fed with 100 mg/kg RJ diet for 14 days. On the 14th day of the experiment, rats in the C+E and RJ+E groups were subjected to exhaustive swimming exercise. Lung oxidative stress indice [malondialdehyde (MDA)] and antioxidant defense systems [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx)] were investigated in this study. Blood samples were collected for analyzing lipid profiles. Treatment of RJ significantly reduced serum levels of total cholesterol, low-density lipoprotein (LDL), and increased high-density lipoprotein (HDL) level. Royal jelly treatment remarkably decreased exercise-induced increases of MDA levels in lung. RJ inverted the exercise-induced increment in SOD activities, but RJ supplementation increased CAT and GSHPx activities. RJ a result, RJ supplementation can prevent elevations of MDA levels in the lung and may have a positive effect on the lung antioxidant defense systems after exhaustive exercise.

Keywords

Royal jelly, oxidative stress, antioxidant enzymes, exhaustive exercise, lung

References

• Aydin, C., Ince, E., Koparan, S., Cangul, I. T., Naziroglu, M., & Ak, F. (2007). Protective effects of long term dietary restriction on swimming exercise‐induced oxidative stress in the liver, heart and kidney of rat. Cell Biochemistry and Function: Cellular biochemistry and its modulation by active agents or disease, 25(2), 129-137.
• Badalzadeh, R., Shaghaghi, M., Mohammadi, M., Dehghan, G., & Mohammadi, Z. (2014). The effect of cinnamon extract and long-term aerobic training on heart function, biochemical alterations and lipid profile following exhaustive exercise in male rats. Advanced pharmaceutical bulletin, 4(Suppl 2), 515.
• Booth, F. W., Roberts, C. K., & Laye, M. J. (2012). Lack of exercise is a major cause of chronic diseases. Comprehensive physiology, 2(2), 1143.
• Brites, F., Zago, V., Verona, J., Muzzio, M. L., Wikinski, R., & Schreier, L. (2006). HDL capacity to inhibit LDL oxidation in well-trained triathletes. Life sciences, 78(26), 3074-3081.
• Chenni, A., Yahia, D. A., Boukortt, F., Prost, J., Lacaille-Dubois, M., & Bouchenak, M. (2007). Effect of aqueous extract of Ajuga iva supplementation on plasma lipid profile and tissue antioxidant status in rats fed a high-cholesterol diet. Journal of ethnopharmacology, 109(2), 207-213.
• Conti, V., Izzo, V., Corbi, G., Russomanno, G., Manzo, V., De Lise, F., Di Donato, A., & Filippelli, A. (2016). Antioxidant supplementation in the treatment of aging-associated diseases. Frontiers in pharmacology, 7, 24.
• Droge, W. (2002). Free radicals in the physiological control of cell function. Physiological reviews, 82(1), 47-95.
• Ensign, W. Y., McNamara, D. J., & Fernandez, M. L. (2002). Exercise improves plasma lipid profiles and modifies lipoprotein composition in guinea pigs. The Journal of nutritional biochemistry, 13(12), 747-753.
• Harman, D. (2006). Free radical theory of aging: an update: increasing the functional life span. Annals of the New York academy of sciences, 1067(1), 10-21.
• Hosseinzadeh, H., Tabassi, S. A. S., Moghadam, N. M., Rashedinia, M., & Mehri, S. (2012). Antioxidant activity of Pistacia vera fruits, leaves and gum extracts. Iranian journal of pharmaceutical research: IJPR, 11(3), 879.
• Hu, F. B., Sigal, R. J., Rich-Edwards, J. W., Colditz, G. A., Solomon, C. G., Willett, W. C., Speizer, F. E., & Manson, J. E. (1999). Walking compared with vigorous physical activity and risk of type 2 diabetes in women: a prospective study. Jama, 282(15), 1433-1439.
• Huang, Q., Ma, S., Tominaga, T., Suzuki, K., & Liu, C. (2018). An 8-Week, Low carbohydrate, high fat, ketogenic diet enhanced exhaustive exercise capacity in mice Part 2: Effect on fatigue recovery, post-exercise biomarkers and anti-oxidation capacity. Nutrients, 10(10), 1339.
• Jen, C. J., Chan, H.-P., & Chen, H.-i. (2002). Chronic exercise improves endothelial calcium signaling and vasodilatation in hypercholesterolemic rabbit femoral artery. Arteriosclerosis, thrombosis, and vascular biology, 22(7), 1219-1224.
• Jones, D. P. (2006). Redefining oxidative stress. Antioxidants & redox signaling, 8(9-10), 1865-1879.
• Kawamura, T., & Muraoka, I. (2018). Exercise-induced oxidative stress and the effects of antioxidant intake from a physiological viewpoint. Antioxidants, 7(9), 119.
• Li, J., Feng, M., Begna, D., Fang, Y., & Zheng, A. (2010). Proteome comparison of hypopharyngeal gland development between Italian and royal jelly producing worker honeybees (Apis mellifera L.). Journal of proteome research, 9(12), 6578-6594.
• Lin, W.-t., Yang, S.-c., Chen, K.-t., Huang, C.-c., & Lee, N.-y. (2005). Protective effects of L-Arginine on pulmonary oxidative stress and anti-oxidant defenses during exhaustive exercise in rats. Acta Pharmacologica Sinica, 26(8), 992-999.
• Liu, Z., Zhou, T., Ziegler, A. C., Dimitrion, P., & Zuo, L. (2017). Oxidative stress in neurodegenerative diseases: from molecular mechanisms to clinical applications. Oxidative medicine and cellular longevity, 2017.
• Malaguti, M., Angeloni, C., Garatachea, N., Baldini, M., Leoncini, E., Collado, P. S., Teti, G., Falconi, M., Gonzalez-Gallego, J., & Hrelia, S. (2009). Sulforaphane treatment protects skeletal muscle against damage induced by exhaustive exercise in rats. Journal of Applied Physiology, 107(4), 1028-1036.
• Maleki, V., Jafari-Vayghan, H., Saleh-Ghadimi, S., Adibian, M., Kheirouri, S., & Alizadeh, M. (2019). Effects of Royal jelly on metabolic variables in diabetes mellitus: A systematic review. Complementary therapies in medicine, 43, 20-27.
• Moore, S. C., Lee, I.-M., Weiderpass, E., Campbell, P. T., Sampson, J. N., Kitahara, C. M., Keadle, S. K., Arem, H., De Gonzalez, A. B., & Hartge, P. (2016). Association of leisure-time physical activity with risk of 26 types of cancer in 1.44 million adults. JAMA internal medicine, 176(6), 816-825.
• Ogura, S., & Shimosawa, T. (2014). Oxidative stress and organ damages. Current Hypertension Reports, 16(8), 452.
• Oláh, A., Németh, B. T., Mátyás, C., Horváth, E. M., Hidi, L., Birtalan, E., Kellermayer, D., Ruppert, M., Merkely, G., & Szabó, G. (2015). Cardiac effects of acute exhaustive exercise in a rat model. International journal of cardiology, 182, 258-266.
• Pinho, R. A., Andrades, M. E., Oliveira, M. R., Pirola, A. C., Zago, M. S., Silveira, P. C., Dal-Pizzol, F., & Moreira, J. C. F. (2006). Imbalance in SOD/CAT activities in rat skeletal muscles submitted to treadmill training exercise. Cell Biology International, 30(10), 848-853.
• Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., & Bitto, A. (2017). Oxidative stress: harms and benefits for human health. Oxidative medicine and cellular longevity, 2017.
• Popovic, L. M., Mitic, N. R., Radic, I., Miric, D., Kisic, B., Krdzic, B., & Djokic, T. (2012). The effect of exhaustive exercise on oxidative stress generation and antioxidant defense in guinea pigs. Advances in Clinical and Experimental Medicine, 21(3), 313-320.
• Prigol, M., Luchese, C., & Nogueira, C. W. (2009). Antioxidant effect of diphenyl diselenide on oxidative stress caused by acute physical exercise in skeletal muscle and lungs of mice. Cell Biochemistry and Function: Cellular biochemistry and its modulation by active agents or disease, 27(4), 216-222.
• Radak, Z., Zhao, Z., Koltai, E., Ohno, H., & Atalay, M. (2013). Oxygen consumption and usage during physical exercise: the balance between oxidative stress and ROS-dependent adaptive signaling. Antioxidants & redox signaling, 18(10), 1208-1246.
• Reddy, K. V., Kumar, T. C., Prasad, M., & Reddanna, P. (1998). Pulmonary lipid peroxidation and antioxidant defenses during exhaustive physical exercise: the role of vitamin E and selenium. Nutrition, 14(5), 448-451.
• Reimers, C. D., Knapp, G., & Reimers, A. K. (2012). Does physical activity increase life expectancy? A review of the literature. Journal of aging research, 2012.
• Sargazi, Z., Nikravesh, M. R., Jalali, M., Sadeghnia, H., Anbarkeh, F. R., & Mohammadzadeh, L. (2015). Gender-related differences in sensitivity to diazinon in gonads of adult rats and the protective effect of vitamin E. International Journal of Women's Health and Reproduction Sciences, 3(1), 40-47.
• Sies, H. (2015). Oxidative stress: a concept in redox biology and medicine. Redox biology, 4, 180-183.
• SIĞ, A. K., Özlem, Ö.-S., & Güney, M. (2019). Royal jelly: a natural therapeutic? Ortadoğu Tıp Dergisi, 11(3), 333-341.
• Silici, S., Ekmekcioglu, O., Eraslan, G., & Demirtas, A. (2009). Antioxidative effect of royal jelly in cisplatin-induced testes damage. Urology, 74(3), 545-551.
• Simioni, C., Zauli, G., Martelli, A. M., Vitale, M., Sacchetti, G., Gonelli, A., & Neri, L. M. (2018). Oxidative stress: role of physical exercise and antioxidant nutraceuticals in adulthood and aging. Oncotarget, 9(24), 17181.
• Stanton, T., Haluska, B. A., Leano, R., Marwick, T. H., & Investigators, C. (2014). Hemodynamic benefit of rest and exercise optimization of cardiac resynchronization therapy. Echocardiography, 31(8), 980-988.
• Suzuki, K. (2019). Chronic inflammation as an immunological abnormality and effectiveness of exercise. Biomolecules, 9(6), 223.
• Suzuki, K., Tominaga, T., Ruhee, R. T., & Ma, S. (2020). Characterization and modulation of systemic inflammatory response to exhaustive exercise in relation to oxidative stress. Antioxidants, 9(5), 401.
• Tan, B. L., Norhaizan, M. E., Huynh, K., Heshu, S. R., Yeap, S. K., Hazilawati, H., & Roselina, K. (2015). Water extract of brewers’ rice induces apoptosis in human colorectal cancer cells via activation of caspase-3 and caspase-8 and downregulates the Wnt/β-catenin downstream signaling pathway in brewers’ rice-treated rats with azoxymethane-induced colon carcinogenesis. BMC Complementary and Alternative Medicine, 15(1), 1-14.
• Taniyama, Y., & Griendling, K. K. (2003). Reactive oxygen species in the vasculature: molecular and cellular mechanisms. Hypertension, 42(6), 1075-1081.
• Taysi, S., Oztasan, N., Efe, H., Polat, M., Gumustekin, K., Siktar, E., Canakci, E., Akcay, F., Dane, S., & Gul, M. (2008). Endurance training attenuates the oxidative stress due to acute exhaustive exercise in rat liver. Acta Physiologica Hungarica, 95(4), 337-347.
• Thomas, D., & Marshall, K. (1988). Effects of repeated exhaustive exercise on myocardial subcellular membrane structures. International journal of sports medicine, 9(04), 257-260.
• VinÑa, J., Gomez‐Cabrera, M. C., Lloret, A., Marquez, R., Minana, J. B., Pallardó, F. V., & Sastre, J. (2000). Free radicals in exhaustive physical exercise: mechanism of production, and protection by antioxidants. IUBMB life, 50(4‐5), 271-277.
• Viuda‐Martos, M., Ruiz‐Navajas, Y., Fernández‐López, J., & Pérez‐Álvarez, J. (2008). Functional properties of honey, propolis, and royal jelly. Journal of food science, 73(9), R117-R124.
• Xiong, Y., Xiong, Y., Wang, Y., Zhao, Y., Li, Y., Ren, Y., Wang, R., Zhao, M., Hao, Y., & Liu, H. (2018). Exhaustive-exercise-induced oxidative stress alteration of erythrocyte oxygen release capacity. Canadian journal of physiology and pharmacology, 96(9), 953-962.
• Xu, J., & Li, Y. (2012). Effects of salidroside on exhaustive exercise‑induced oxidative stress in rats. Molecular medicine reports, 6(5), 1195-1198.
• Yada, K., Roberts, L. A., Oginome, N., & Suzuki, K. (2020). Effect of acacia polyphenol supplementation on exercise-induced oxidative stress in mice liver and skeletal muscle. Antioxidants, 9(1), 29.
• Yang, D. K., Lee, S.-J., Adam, G. O., & Kim, S.-J. (2020). Aralia continentalis kitagawa Extract Attenuates the Fatigue Induced by Exhaustive Exercise through Inhibition of Oxidative Stress. Antioxidants, 9(5), 379.
• Zhang, H., Liu, M., Zhang, Y., & Li, X. (2019). Trimetazidine attenuates exhaustive exercise-induced myocardial injury in rats via regulation of the Nrf2/NF-κB signaling pathway. Frontiers in pharmacology, 10, 175.