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Kabak Çekirdeği Yağının Kompleks Koaservasyon Yöntemi ile Mikroenkapsülasyonu

Year 2021, Volume: 11 Issue: 2, 91 - 97, 15.12.2021

Abstract

Cucurbitaceae familyasının alt türleri arasında yer alan kabak bitkisi, farklı türleri olan ve tohumları da tüketilen tarımsal bir üründür.
Zengin bir yağ asidi kaynağı olması nedeniyle kabak çekirdeği yağı sadece gıda endüstrisinde değil, ilaç ve kozmetik endüstrisi içinde
önem taşımaktadır. Bu çalışmamızda kabak çekirdeği yağı yanıt yüzey metodolojisi (YYM) ile oluşturulan bir deney setinde kompleks
koaservasyon yöntemi ile enkapsülasyonlanmıştır. YYM’de bağımsız değişkenler olarak sıcaklık (50,00 0C - 60,00 0C) ve pH (3,80 -
4,20), bağımlı değişken olarak ise deneysel olarak hesaplanan enkapsülasyon verimi (%EV) kullanılmıştır. Deney setindeki en yüksek
%EV = %79,63 olarak bulunmuş ve 55,00 0C ve 4,00 pH değerinde ulaşılmıştır. YYM için hesaplanan ANOVA sonuçlarında; R2 =
%99,44, uyum eksikliğinin p değeri = 0,11 bulunmuştur. Uyum eksikliğinin p değerinin (p>0,05) olması model ile verinin uyuştuğunu
göstermektedir. Her deney seti için enkapsüle edilen numuneler optik mikroskop ile karakterize edilmiştir. Çalışmamızın sonucunda
girdi değişkenleri ile cevap yanıtı arasındaki ilişki literatür yardımıyla değerlendirilmiştir

References

  • Abdel-Rahman, M. K., 2006. Effect of pumpkin seed (Cucurbita pepo L.) diets on benign prostatic hyperplasia (BPH): chemical and morphometric evaluation in rats. World J Chem, 1(1), 33-40.
  • Acharya, A. S., Prakash, A., Saxena, P., Nigam, A., 2013. Sampling: Why and how of it. Indian Journal of Medical Specialties, 4(2), 330-333, DOI: http://dx.doi.org/10.7713/ijms.2013.0032.
  • Aziz, S., Gill, J., Dutilleul, P., Neufeld, R., Kermasha, S., 2014. Microencapsulation of krill oil using complex coacervation. Journal of Microencapsulation, 31(8), 774-784, DOI: 10.3109/02652048.2014.932028.
  • Bayram O., Köksal E., Danaş F., Göde F., Erzengin Ö. U., 2019. A Statistical Investigation of the Effects of Different Surfactant Concentrations on the Capsule Size in the Encapsulation of Grapefruit (Citrus Paradisi Macf.) Peel Oil By Complex Coacervation Method. 2nd International Health Science And Life Congress, 24–27 April, Burdur, 486-497.
  • Caili, F. U., Huan, S., Quanhong, L. I., 2006. A review on pharmacological activities and utilization technologies of pumpkin. Plant foods for human nutrition, 61(2), 70-77, DOI: 10.1007/s11130-006-0016-6.
  • Evranuz, E. Ö., Arduzlar-Kağan, D., 2015. Winter Squash and Pumpkins. In Handbook of Vegetable Preservation and Processing, CRC Press, 692-711.
  • Gohari, A. A., Farhoosh, R., Haddad, K. M., 2011. Chemical composition and physicochemical properties of pumpkin seeds (Cucurbita pepo Subsp. pepo Var. Styriaka) grown in Iran. Journal Of Agricultural Science And Technology , 13, 1053-1063.
  • Hu, L., Zhang, J., Hu, Q., Gao, N., Wang, S., Sun, Y., Yang, X. 2016. Microencapsulation Of Brucea Javanica Oil: Characterization, Stability And Optimization Of Spray Drying Conditions. Journal of Drug Delivery Science and Technology, 36, 46-54, DOI: http://dx.doi.org/10.1016/j.jddst.2016.09.008
  • Jyothi, N. V. N., Prasanna, P. M., Sakarkar, S. N., Prabha, K. S., Ramaiah, P. S., Srawan, G. Y., 2010. Microencapsulation techniques, factors influencing encapsulation efficiency. Journal of microencapsulation, 27(3), 187-197, DOI: 10.3109/02652040903131301.
  • Köksal, E. 2016. Kompleks Koaservasyon Yöntemi İle E Vitamini İçeren Mikrokapsül Üretimi. Yüksek Lisans Tezi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, 69s.
  • Köksal, E., Bayram O., Danaş, F., Göde, F., Aktaş A. H., 2019. Shea Yağının Mikrokapsüllenmesi: Optimizasyon Ve Karakterizasyon Çalışması. 2. Uluslararası Sağlık Bilimleri Ve Yaşam Kongresi, 24-27 Nisan, Burdur, 737-748.
  • Kwak, H. S., 2014. Nano- and Microencapsulation for Foods. Wiley & Sons, 1-14, DOI: 10.1002/9781118292327.
  • Murkovic, M., Pfannhauser, W., 2000. Stability of pumpkin seed oil. European Journal of Lipid Science and Technology, 102(10), 607-611, https://doi.org/10.1002/1438-9312(200010)102:10<607::AID-EJLT607>3.0.CO;2-E
  • Myers, R. H., Montgomery, D. C., Anderson-Cook, C. M., 2016. Response surface methodology: process and product optimization using designed experiments. John Wiley & Sons.
  • Neđeral, S., Petrović, M., Vincek, D., Pukec, D., Škevin, D., Kraljić, K., Obranović, M., 2014. Variance of quality parameters and fatty acid composition in pumpkin seed oil during three crop seasons. Industrial Crops and Products, 60, 15-21, DOI: http://dx.doi.org/10.1016/j.indcrop.2014.05.044
  • Nesterenko, A., Alric, I., Silvestre, F., Durrieu, V., 2014. Comparative study of encapsulation of vitamins with native and modified soy protein. Food Hydrocolloids, 38, 172-179, DOI: http://dx.doi.org/10.1016/j.foodhyd.2013.12.011.
  • Ocak, B., 2012. Complex coacervation of collagen hydrolysate extracted from leather solid wastes and chitosan for controlled release of lavender oil. Journal of Environmental Management, 100, 22-28, DOI: 10.1016/j.jenvman.2012.01.026
  • Patrickab, K. E., Abbasa, S., Lva, Y., Ntsamacd, I. S. B., Zhanga, X. 2013. Microencapsulation By Complex Coacervation Of Fish Oil Using Gelatin/SDS/NaCMC. Pakistan Journal of Food Sciences, 23(1), 17-25.
  • Rajam, R., Anandharamakrishnan, C., 2015. Microencapsulation of Lactobacillus plantarum (MTCC 5422) with fructooligosaccharide as wall material by spray drying. LWT-Food Science and Technology, 60(2), 773-780, DOI: https://doi.org/10.1016/j.lwt.2014.09.062
  • Sharifi, F., Hadizadeh, F., Sadeghi, F., Hamed Mosavian, M. T., Zarei, C., 2016. Process Optimization, Physical Properties, and Environmental Stability of an α-Tocopherol Nanocapsule Preparation Using Complex Coacervation Method and Full Factorial Design. Chemical Engineering Communications, 203(1), 64-74, DOI: https://doi.org/10.1080/00986445.2014.973941
  • Shaddel, R., Hesari, J., Azadmard-Damichi, S., Hamishehkar, H., Fathi-Achachlouei, B., Huang, Q., 2018. Use of gelatin and gum Arabic for encapsulation of black raspberry anthocyanins by complex coacervation. International Journal of Biological Macromolecules, 107, 1800-1810, DOI: https://doi.org/10.1016/j.ijbiomac.2017.10.044
  • Stevenson, D. G., Eller, F. J., Wang, L., Jane, J. L., Wang, T., Inglett, G. E., 2007. Oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars. Journal of agricultural and food chemistry, 55(10), 4005-4013, https://doi.org/10.1021/jf0706979
  • Timilsena, Y. P., Akanbi, T. O., Khalid, N., Adhikari, B., Barrow, C. J., 2019. Complex coacervation: Principles, mechanisms and applications in microencapsulation. International journal of biological macromolecules, 121, 1276-1286, DOI: https://doi.org/10.1016/j.ijbiomac.2018.10.144
  • Veronezi, C. M., Jorge, N., 2012. Bioactive compounds in lipid fractions of pumpkin (Cucurbita sp) seeds for use in food. Journal of food science, 77(6), 653-657, https://doi.org/10.1111/j.1750-3841.2012.02736.x
  • Yadav, M., Jain, S., Tomar, R., Prasad, G. B. K. S., Yadav, H., 2010. Medicinal and biological potential of pumpkin: an updated review. Nutrition research reviews, 23(2), 184-190, DOI: https://doi.org/10.1017/S0954422410000107
  • Yanmaz, R., Düzeltir, B., 2003. Çekirdek kabağı yetiştiriciliği. Ekin Dergisi, 7(6), 22-24.
  • Zuidam, N. J., Shimoni, E., 2010. Overview of microencapsulates for use in food products or processes and methods to make them. Encapsulation technologies for active food ingredients and food processing, pp. 3-29, DOI: https://link.springer.com/chapter/10.1007%2F978-1-4419-1008-0_2

Microencapsulation of Pumpkin Seed Oil with Complex Coacervation Method

Year 2021, Volume: 11 Issue: 2, 91 - 97, 15.12.2021

Abstract

Pumpkin plant, which is among the subspecies of Cucurbitaceae family, is an agricultural product with different types and seeds
are consumed. Since it is a rich source of fatty acids, pumpkin seed oil is important not only in the food industry, but also in the
pharmaceutical and cosmetics industry. In this study, pumpkin seed oil was encapsulated with complex coacervation method in an
experiment set created with response surface methodology (RSM). Temperature (50.00 0C – 60.00 0C) and pH (3.80 – 4.20) were
used as independent variables in RSM, and experimentally calculated encapsulation efficiency (%EV) was used as dependent variable.
The highest EV% in the experimental set 79.63% and 55.00 0C and 4.00 pH values were reached. In ANOVA results calculated for
RSM; R2 = 99.44%, lack of fit p value = 0.11. The lack of fit p value (p>0.05) indicates that the model matches the data. The samples
encapsulated for each set of experiments were characterized by an optical microscope. As a result of our study, the relationship between
input variables and response was evaluated with the help of the literature.

References

  • Abdel-Rahman, M. K., 2006. Effect of pumpkin seed (Cucurbita pepo L.) diets on benign prostatic hyperplasia (BPH): chemical and morphometric evaluation in rats. World J Chem, 1(1), 33-40.
  • Acharya, A. S., Prakash, A., Saxena, P., Nigam, A., 2013. Sampling: Why and how of it. Indian Journal of Medical Specialties, 4(2), 330-333, DOI: http://dx.doi.org/10.7713/ijms.2013.0032.
  • Aziz, S., Gill, J., Dutilleul, P., Neufeld, R., Kermasha, S., 2014. Microencapsulation of krill oil using complex coacervation. Journal of Microencapsulation, 31(8), 774-784, DOI: 10.3109/02652048.2014.932028.
  • Bayram O., Köksal E., Danaş F., Göde F., Erzengin Ö. U., 2019. A Statistical Investigation of the Effects of Different Surfactant Concentrations on the Capsule Size in the Encapsulation of Grapefruit (Citrus Paradisi Macf.) Peel Oil By Complex Coacervation Method. 2nd International Health Science And Life Congress, 24–27 April, Burdur, 486-497.
  • Caili, F. U., Huan, S., Quanhong, L. I., 2006. A review on pharmacological activities and utilization technologies of pumpkin. Plant foods for human nutrition, 61(2), 70-77, DOI: 10.1007/s11130-006-0016-6.
  • Evranuz, E. Ö., Arduzlar-Kağan, D., 2015. Winter Squash and Pumpkins. In Handbook of Vegetable Preservation and Processing, CRC Press, 692-711.
  • Gohari, A. A., Farhoosh, R., Haddad, K. M., 2011. Chemical composition and physicochemical properties of pumpkin seeds (Cucurbita pepo Subsp. pepo Var. Styriaka) grown in Iran. Journal Of Agricultural Science And Technology , 13, 1053-1063.
  • Hu, L., Zhang, J., Hu, Q., Gao, N., Wang, S., Sun, Y., Yang, X. 2016. Microencapsulation Of Brucea Javanica Oil: Characterization, Stability And Optimization Of Spray Drying Conditions. Journal of Drug Delivery Science and Technology, 36, 46-54, DOI: http://dx.doi.org/10.1016/j.jddst.2016.09.008
  • Jyothi, N. V. N., Prasanna, P. M., Sakarkar, S. N., Prabha, K. S., Ramaiah, P. S., Srawan, G. Y., 2010. Microencapsulation techniques, factors influencing encapsulation efficiency. Journal of microencapsulation, 27(3), 187-197, DOI: 10.3109/02652040903131301.
  • Köksal, E. 2016. Kompleks Koaservasyon Yöntemi İle E Vitamini İçeren Mikrokapsül Üretimi. Yüksek Lisans Tezi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü, 69s.
  • Köksal, E., Bayram O., Danaş, F., Göde, F., Aktaş A. H., 2019. Shea Yağının Mikrokapsüllenmesi: Optimizasyon Ve Karakterizasyon Çalışması. 2. Uluslararası Sağlık Bilimleri Ve Yaşam Kongresi, 24-27 Nisan, Burdur, 737-748.
  • Kwak, H. S., 2014. Nano- and Microencapsulation for Foods. Wiley & Sons, 1-14, DOI: 10.1002/9781118292327.
  • Murkovic, M., Pfannhauser, W., 2000. Stability of pumpkin seed oil. European Journal of Lipid Science and Technology, 102(10), 607-611, https://doi.org/10.1002/1438-9312(200010)102:10<607::AID-EJLT607>3.0.CO;2-E
  • Myers, R. H., Montgomery, D. C., Anderson-Cook, C. M., 2016. Response surface methodology: process and product optimization using designed experiments. John Wiley & Sons.
  • Neđeral, S., Petrović, M., Vincek, D., Pukec, D., Škevin, D., Kraljić, K., Obranović, M., 2014. Variance of quality parameters and fatty acid composition in pumpkin seed oil during three crop seasons. Industrial Crops and Products, 60, 15-21, DOI: http://dx.doi.org/10.1016/j.indcrop.2014.05.044
  • Nesterenko, A., Alric, I., Silvestre, F., Durrieu, V., 2014. Comparative study of encapsulation of vitamins with native and modified soy protein. Food Hydrocolloids, 38, 172-179, DOI: http://dx.doi.org/10.1016/j.foodhyd.2013.12.011.
  • Ocak, B., 2012. Complex coacervation of collagen hydrolysate extracted from leather solid wastes and chitosan for controlled release of lavender oil. Journal of Environmental Management, 100, 22-28, DOI: 10.1016/j.jenvman.2012.01.026
  • Patrickab, K. E., Abbasa, S., Lva, Y., Ntsamacd, I. S. B., Zhanga, X. 2013. Microencapsulation By Complex Coacervation Of Fish Oil Using Gelatin/SDS/NaCMC. Pakistan Journal of Food Sciences, 23(1), 17-25.
  • Rajam, R., Anandharamakrishnan, C., 2015. Microencapsulation of Lactobacillus plantarum (MTCC 5422) with fructooligosaccharide as wall material by spray drying. LWT-Food Science and Technology, 60(2), 773-780, DOI: https://doi.org/10.1016/j.lwt.2014.09.062
  • Sharifi, F., Hadizadeh, F., Sadeghi, F., Hamed Mosavian, M. T., Zarei, C., 2016. Process Optimization, Physical Properties, and Environmental Stability of an α-Tocopherol Nanocapsule Preparation Using Complex Coacervation Method and Full Factorial Design. Chemical Engineering Communications, 203(1), 64-74, DOI: https://doi.org/10.1080/00986445.2014.973941
  • Shaddel, R., Hesari, J., Azadmard-Damichi, S., Hamishehkar, H., Fathi-Achachlouei, B., Huang, Q., 2018. Use of gelatin and gum Arabic for encapsulation of black raspberry anthocyanins by complex coacervation. International Journal of Biological Macromolecules, 107, 1800-1810, DOI: https://doi.org/10.1016/j.ijbiomac.2017.10.044
  • Stevenson, D. G., Eller, F. J., Wang, L., Jane, J. L., Wang, T., Inglett, G. E., 2007. Oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars. Journal of agricultural and food chemistry, 55(10), 4005-4013, https://doi.org/10.1021/jf0706979
  • Timilsena, Y. P., Akanbi, T. O., Khalid, N., Adhikari, B., Barrow, C. J., 2019. Complex coacervation: Principles, mechanisms and applications in microencapsulation. International journal of biological macromolecules, 121, 1276-1286, DOI: https://doi.org/10.1016/j.ijbiomac.2018.10.144
  • Veronezi, C. M., Jorge, N., 2012. Bioactive compounds in lipid fractions of pumpkin (Cucurbita sp) seeds for use in food. Journal of food science, 77(6), 653-657, https://doi.org/10.1111/j.1750-3841.2012.02736.x
  • Yadav, M., Jain, S., Tomar, R., Prasad, G. B. K. S., Yadav, H., 2010. Medicinal and biological potential of pumpkin: an updated review. Nutrition research reviews, 23(2), 184-190, DOI: https://doi.org/10.1017/S0954422410000107
  • Yanmaz, R., Düzeltir, B., 2003. Çekirdek kabağı yetiştiriciliği. Ekin Dergisi, 7(6), 22-24.
  • Zuidam, N. J., Shimoni, E., 2010. Overview of microencapsulates for use in food products or processes and methods to make them. Encapsulation technologies for active food ingredients and food processing, pp. 3-29, DOI: https://link.springer.com/chapter/10.1007%2F978-1-4419-1008-0_2
There are 27 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Research Article
Authors

Emel Moral 0000-0002-8158-5797

Okan Bayram 0000-0002-1748-9354

Elif Köksal 0000-0001-5131-3531

Fatma Danaş 0000-0002-2233-5018

Fethiye Göde 0000-0002-3008-1353

Publication Date December 15, 2021
Published in Issue Year 2021 Volume: 11 Issue: 2

Cite

APA Moral, E., Bayram, O., Köksal, E., Danaş, F., et al. (2021). Kabak Çekirdeği Yağının Kompleks Koaservasyon Yöntemi ile Mikroenkapsülasyonu. Karaelmas Fen Ve Mühendislik Dergisi, 11(2), 91-97. https://doi.org/10.7212/karaelmasfen.821902
AMA Moral E, Bayram O, Köksal E, Danaş F, Göde F. Kabak Çekirdeği Yağının Kompleks Koaservasyon Yöntemi ile Mikroenkapsülasyonu. Karaelmas Fen ve Mühendislik Dergisi. December 2021;11(2):91-97. doi:10.7212/karaelmasfen.821902
Chicago Moral, Emel, Okan Bayram, Elif Köksal, Fatma Danaş, and Fethiye Göde. “Kabak Çekirdeği Yağının Kompleks Koaservasyon Yöntemi Ile Mikroenkapsülasyonu”. Karaelmas Fen Ve Mühendislik Dergisi 11, no. 2 (December 2021): 91-97. https://doi.org/10.7212/karaelmasfen.821902.
EndNote Moral E, Bayram O, Köksal E, Danaş F, Göde F (December 1, 2021) Kabak Çekirdeği Yağının Kompleks Koaservasyon Yöntemi ile Mikroenkapsülasyonu. Karaelmas Fen ve Mühendislik Dergisi 11 2 91–97.
IEEE E. Moral, O. Bayram, E. Köksal, F. Danaş, and F. Göde, “Kabak Çekirdeği Yağının Kompleks Koaservasyon Yöntemi ile Mikroenkapsülasyonu”, Karaelmas Fen ve Mühendislik Dergisi, vol. 11, no. 2, pp. 91–97, 2021, doi: 10.7212/karaelmasfen.821902.
ISNAD Moral, Emel et al. “Kabak Çekirdeği Yağının Kompleks Koaservasyon Yöntemi Ile Mikroenkapsülasyonu”. Karaelmas Fen ve Mühendislik Dergisi 11/2 (December 2021), 91-97. https://doi.org/10.7212/karaelmasfen.821902.
JAMA Moral E, Bayram O, Köksal E, Danaş F, Göde F. Kabak Çekirdeği Yağının Kompleks Koaservasyon Yöntemi ile Mikroenkapsülasyonu. Karaelmas Fen ve Mühendislik Dergisi. 2021;11:91–97.
MLA Moral, Emel et al. “Kabak Çekirdeği Yağının Kompleks Koaservasyon Yöntemi Ile Mikroenkapsülasyonu”. Karaelmas Fen Ve Mühendislik Dergisi, vol. 11, no. 2, 2021, pp. 91-97, doi:10.7212/karaelmasfen.821902.
Vancouver Moral E, Bayram O, Köksal E, Danaş F, Göde F. Kabak Çekirdeği Yağının Kompleks Koaservasyon Yöntemi ile Mikroenkapsülasyonu. Karaelmas Fen ve Mühendislik Dergisi. 2021;11(2):91-7.