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Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells

Year 2022, Volume: 12 Issue: 1, 38 - 46, 01.06.2022

Yeliz Yalçın , İshak Özel Tekin , Rahime Seda Tığlı Aydın

Abstract

Tumor hypoxia is a restrictive factor for cancer treatment. Ozone therapy, used to decrease or prevent the hypoxia in tumor. In this
study, it is aimed to use ozonized oil nanoemulsions (OZNEs) to overcome the limitations of ozone therapy in cancer research. The
influence of OZNE against cancer cell lines is evaluated by Reactive Oxygen Species (ROS) and ɣH2AX assays. For both B-16
melanoma and OV-90 cell lines, ROS production levels increased due to the increase in OZNE doses (0.85% to 14.88% and 1.94%
to 58.58% for B-16 and OV-90 cell lines, respectively). 5-fold higher ɣH2AX cell positivity’s determined in OV-90 cells compared to
B-16 melanoma cells. OZNE treatment could provide a new effective method to damage cancer cells in the future.

Keywords

Ozonized oil nano ɣH2AX ROS B-16 melanoma OV-90 ovarian

Supporting Institution

Bülent Ecevit University Scientific Research Projects

Project Number

2016-39971044-02

References

  • Alarifi, S., Ali, D., Alkahtani, S., & Almeer, R. S. 2017. ROS-mediated apoptosis and genotoxicity induced by palladium nanoparticles in human skin malignant melanoma cells. Oxi. Medic. and Cell. Longe., 2017:1-10.http://doi.org/10.1155/2017/8439098.
  • Bassing, C. H., Suh, H., Ferguson, D. O., Chua, K. F., Manis, J., Eckersdorff, M., Alt, F. W. 2003. Histone H2AX: a dosage-dependent suppressor of oncogenic translocations and tumors. Cell Stem Cell, 114(3): 359-370. http://doi.org/10.1016/s0092-8674(03)00566-x.
  • Bayr, H. 2005. Reactive oxygen species. Crit. Care Medi., 33(12): S498-S501. doi:10.1097/01.CCM.0000186787.64500.12
  • Bhardwaj, M., Kim, N.-H., Paul, S., Jakhar, R., Han, J., Kang, S. C. 2016. 5-Hydroxy-7-methoxyflavone triggers mitochondrial-associated cell death via reactive oxygen species signaling in human colon carcinoma cells. PloS one, 11(4): e0154525. doi:10.1371/journal.pone.0154525.
  • Brizel, D. M., Scully, S. P., Harrelson, J. M., Layfield, L. J., Bean, J. M., Prosnitz, L. R., Dewhirst, M. 1996. Tumor oxygenation predicts for the likelihood of distant metastases in human soft tissue sarcoma. Cancer Res., 56(5): 941-943.
  • Clavo, B., Pérez, J. L., López, L., Suárez, G., Lloret, M., Rodríguez, V., Robaina, F. 2004. Ozone Therapy for Tumor Oxygenation: a Pilot Study. Evid Based Comp. Alter. Med., 1(1): 93-98. http:///doi.org/10.1093/ecam/neh009.
  • Gray, L. H., Conger, A. D., Ebert, M., Hornsey, S., Scott, O. 1953. The concentration of oxygen dissolved in tissues at the time of irradiation as a factor in radiotherapy. The Brit. J. Radio., 26(312): 638-648.
  • Jackson, S. P. 2002. Sensing and repairing DNA double-strand breaks. Carcinogenesiss, 23(5): 687-696. doi:10.1093/carcin/23.5.687.
  • Khan, I., Bahuguna, A., Kumar, P., Bajpai, V. K., Kang, S. C. 2018. In vitro and in vivo antitumor potential of carvacrol nanoemulsion against human lung adenocarcinoma A549 cells via mitochondrial mediated apoptosis. Sci.Rep.,8(1): 1-15.
  • Klestadt, D., Laval-Gilly, P., Foucaud, L. 2005. Influences of ozone exposure upon macrophage responsivity to N-formyl-methionyl-leucyl-phenylalanine: mobility and metabolic changes. Oxico. In Vitro, 19(2): 199-206.
  • Kuo, L. J., Yang, L.-X. 2008. γ-H2AX-a novel biomarker for DNA double-strand breaks. Int. Inst. Antica. Res., 22(3): 305-309. doi:10.3390/cancers12113288.
  • Nikitaki, Z., Pariset, E., Sudar, D., Costes, S. V., Georgakilas, A. G. 2020. In Situ Detection of Complex DNA Damage Using Microscopy: A Rough Road Ahead. Cancers, 12(11): 3288.
  • Plasswilm, L., Tannapfel, A., Cordes, N., Demir, R., Hoeper, K., Bauer, J., Hoeper, J. 2000. Hypoxia-induced tumour cell migration in an in vivo chicken model. Pathobio., 68(3): 99-105. doi:10.1159/000055909.
  • Reczek, C. R., Chandel, N. S. 2015. ROS-dependent signal transduction. Curr. Op. Cell Bio., 33: 8-13. doi:10.1016/j.ceb.2014.09.010.
  • Rogakou, P, E., Pilch, D. R., Orr, A. H., Ivanova, V. S., Bonner, W. M. 1998. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J. Bio. Chem., 273(10): 5858-5868.
  • Serio, F., Pizzolante, G., Cozzolino, G., D’Alba, M., Bagordo, F., De Giorgi, M., De Donno, A. 2017. A new formulation based on ozonated sunflower seed oil: in vitro antibacterial and safety evaluation. Ozone: Sci. Eng., 39(3): 139-147.
  • Sfikas, A., Batsi, C., Tselikou, E., Vartholomatos, G., Monokrousos, N., Pappas, P., Gorgoulis, V. G. 2012. The canonical NF-κB pathway differentially protects normal and human tumor cells from ROS-induced DNA damage. Cell. Sign., 24(11): 2007-2023. doi:10.1016/j.cellsig.2012.06.010.
  • Simon, H.-U., Haj-Yehia, A., Levi-Schaffer, F. 2000. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis, 5(5): 415-418.
  • Simonetti, V., Quagliariello, V., Giustetto, P., Franzini, M., Iaffaioli, R. V. 2017. Association of ozone with 5-fluorouracil and cisplatin in regulation of human colon cancer cell viability: in vitro anti-inflammatory properties of ozone in colon cancer cells exposed to lipopolysaccharides. Evidence-Based Complementary Alternative Medicine, 2017.
  • Sonoda, Eiichiro, Hochegger, H., Saberi, A., Taniguchi, Y., Takeda, S. 2006. Differential usage of non-homologous end-joining and homologous recombination in double strand break repair. DNA Repair, 5(9-10): 1021-1029. doi:10.1016/j.dnarep.2006.05.022.
  • Stadtman, E. R., & Berlett, B. S. 1997. Reactive oxygen-mediated protein oxidation in aging and disease. Chem. Res. in Toxic., 10(5): 485-494.
  • Stiff, T., O’Driscoll, M., Rief, N., Iwabuchi, K., Löbrich, M., Jeggo, P. A. 2004. ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation. Cancer Res., 64(7): 2390-2396. doi:10.1158/0008-5472.CAN-03-3207
  • Stoker, G. 1902. Ozone in Chronic Middle-ear Deafness. The Lancet, 160(4131): 1187-1188.
  • Strickland, A. F., Perkins, W. S. 1995. Decolorization of continuous dyeing wastewater by ozonation. Text. Chem. Color., 27(5): 11-15.
  • Sweet, F., Kao, M., Lee, S., Hagar, W., Sweet, W. 1980. Ozone selectively inhibits growth of human cancer cells. Scie., 209(4459): 931-933. doi:10.1126/science.7403859 %J Science.
  • Tang, Z. m., Liu, Y. y., Ni, D. l., Zhou, J. j., Zhang, M., Zhao, P., Bu, W. 2020. Biodegradable Nanoprodrugs:“Delivering” ROS to Cancer Cells for Molecular Dynamic Therapy. Adv. Mat., 32(4): 1904011. doi:10.1002/adma.201904011.
  • Tığlı Aydın, R. S., Kazancı, F. 2018. Synthesis and characterization of ozonated oil nanoemulsions. J. Ame. Oil Chem. Soc., 95(11): 1385-1398. doi:10.1002/aocs.12150.
  • Travagli, V., Zanardi, I., Valacchi, G.,Bocci, V. 2010. Ozone and ozonated oils in skin diseases: A Review. 2010.
  • Van Meir, 1996. Hypoxia-mediated selection of cells with diminished apoptotic potential in solid tumours. Neurosurg., 39(4): 878-879.
  • Yalçın, Y., Tekin, I. O., Aydın, R. S. 2021. Influence of Ozonized Oil Nanoemulsions on B-16 Melanoma Cells: An in Vitro Study. J. Biomat. and Nanobiotech., (12): 49-56. doi:doi: 10.4236/jbnb.2021.124005.
  • Young, S., Marshall, R., Hill, R. 1988. Hypoxia induces DNA overreplication and enhances metastatic potential of murine tumor cells. Proce.Natio. Acad. Sci., 85(24): 9533-9537. http:doi.org/10.1073/pnas.85.24.9533.

Nano Ozon Yağının B-16 Melanoma ve OV-90 Over Hücrelerinde ROS Üretimi ve ɣH2AX Hücre Pozitifliğini Tetiklemesi

Year 2022, Volume: 12 Issue: 1, 38 - 46, 01.06.2022

Yeliz Yalçın , İshak Özel Tekin , Rahime Seda Tığlı Aydın

Abstract

Tumör hipoksisi, kanser tedavisinde tedaviyi kısıtlayan bir faktördür. Ozon terapi, tumor hipoksisini azaltmak ya da önlemek amaçlı
kullanılır. Bu çalışmada, kanser tedavisinde ozon terapinin kısıtlamalarının üstesinden gelmek için, ozon yağı nanoemülsiyonu
(OZNE) kullanımı amaçlanmaktadır. OZNE’nin kanser hücre hatlarına olan etkisi, Reaktif Oksijen Türevleri (ROS) ve ɣH2AX
testleri ile değerlendirilmiştir. B-16 melanoma ve OV-90 over hücre hatlarının her ikisinde de, ROS üretimi OZNE doz artışı ile
artmıştır (B-16 melanoma için %0.85’den %14.88’e, OV-90 over için %1.94’den %58.58’e), OV-90 over hücreleri ɣH2AX hücre
pozitifliği B-16 melanom ile kıyaslandığında 5 kat fazla artış göstermiştir. OZNE tedavisi gelecekte kanser hücrelerine hasar verecek
yeni etkili bir yöntem sağlayabilir.

Keywords

Ozon yağı nano ɣH2AX ROS B-16 melanoma OV-90 over

Project Number

2016-39971044-02

References

  • Alarifi, S., Ali, D., Alkahtani, S., & Almeer, R. S. 2017. ROS-mediated apoptosis and genotoxicity induced by palladium nanoparticles in human skin malignant melanoma cells. Oxi. Medic. and Cell. Longe., 2017:1-10.http://doi.org/10.1155/2017/8439098.
  • Bassing, C. H., Suh, H., Ferguson, D. O., Chua, K. F., Manis, J., Eckersdorff, M., Alt, F. W. 2003. Histone H2AX: a dosage-dependent suppressor of oncogenic translocations and tumors. Cell Stem Cell, 114(3): 359-370. http://doi.org/10.1016/s0092-8674(03)00566-x.
  • Bayr, H. 2005. Reactive oxygen species. Crit. Care Medi., 33(12): S498-S501. doi:10.1097/01.CCM.0000186787.64500.12
  • Bhardwaj, M., Kim, N.-H., Paul, S., Jakhar, R., Han, J., Kang, S. C. 2016. 5-Hydroxy-7-methoxyflavone triggers mitochondrial-associated cell death via reactive oxygen species signaling in human colon carcinoma cells. PloS one, 11(4): e0154525. doi:10.1371/journal.pone.0154525.
  • Brizel, D. M., Scully, S. P., Harrelson, J. M., Layfield, L. J., Bean, J. M., Prosnitz, L. R., Dewhirst, M. 1996. Tumor oxygenation predicts for the likelihood of distant metastases in human soft tissue sarcoma. Cancer Res., 56(5): 941-943.
  • Clavo, B., Pérez, J. L., López, L., Suárez, G., Lloret, M., Rodríguez, V., Robaina, F. 2004. Ozone Therapy for Tumor Oxygenation: a Pilot Study. Evid Based Comp. Alter. Med., 1(1): 93-98. http:///doi.org/10.1093/ecam/neh009.
  • Gray, L. H., Conger, A. D., Ebert, M., Hornsey, S., Scott, O. 1953. The concentration of oxygen dissolved in tissues at the time of irradiation as a factor in radiotherapy. The Brit. J. Radio., 26(312): 638-648.
  • Jackson, S. P. 2002. Sensing and repairing DNA double-strand breaks. Carcinogenesiss, 23(5): 687-696. doi:10.1093/carcin/23.5.687.
  • Khan, I., Bahuguna, A., Kumar, P., Bajpai, V. K., Kang, S. C. 2018. In vitro and in vivo antitumor potential of carvacrol nanoemulsion against human lung adenocarcinoma A549 cells via mitochondrial mediated apoptosis. Sci.Rep.,8(1): 1-15.
  • Klestadt, D., Laval-Gilly, P., Foucaud, L. 2005. Influences of ozone exposure upon macrophage responsivity to N-formyl-methionyl-leucyl-phenylalanine: mobility and metabolic changes. Oxico. In Vitro, 19(2): 199-206.
  • Kuo, L. J., Yang, L.-X. 2008. γ-H2AX-a novel biomarker for DNA double-strand breaks. Int. Inst. Antica. Res., 22(3): 305-309. doi:10.3390/cancers12113288.
  • Nikitaki, Z., Pariset, E., Sudar, D., Costes, S. V., Georgakilas, A. G. 2020. In Situ Detection of Complex DNA Damage Using Microscopy: A Rough Road Ahead. Cancers, 12(11): 3288.
  • Plasswilm, L., Tannapfel, A., Cordes, N., Demir, R., Hoeper, K., Bauer, J., Hoeper, J. 2000. Hypoxia-induced tumour cell migration in an in vivo chicken model. Pathobio., 68(3): 99-105. doi:10.1159/000055909.
  • Reczek, C. R., Chandel, N. S. 2015. ROS-dependent signal transduction. Curr. Op. Cell Bio., 33: 8-13. doi:10.1016/j.ceb.2014.09.010.
  • Rogakou, P, E., Pilch, D. R., Orr, A. H., Ivanova, V. S., Bonner, W. M. 1998. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J. Bio. Chem., 273(10): 5858-5868.
  • Serio, F., Pizzolante, G., Cozzolino, G., D’Alba, M., Bagordo, F., De Giorgi, M., De Donno, A. 2017. A new formulation based on ozonated sunflower seed oil: in vitro antibacterial and safety evaluation. Ozone: Sci. Eng., 39(3): 139-147.
  • Sfikas, A., Batsi, C., Tselikou, E., Vartholomatos, G., Monokrousos, N., Pappas, P., Gorgoulis, V. G. 2012. The canonical NF-κB pathway differentially protects normal and human tumor cells from ROS-induced DNA damage. Cell. Sign., 24(11): 2007-2023. doi:10.1016/j.cellsig.2012.06.010.
  • Simon, H.-U., Haj-Yehia, A., Levi-Schaffer, F. 2000. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis, 5(5): 415-418.
  • Simonetti, V., Quagliariello, V., Giustetto, P., Franzini, M., Iaffaioli, R. V. 2017. Association of ozone with 5-fluorouracil and cisplatin in regulation of human colon cancer cell viability: in vitro anti-inflammatory properties of ozone in colon cancer cells exposed to lipopolysaccharides. Evidence-Based Complementary Alternative Medicine, 2017.
  • Sonoda, Eiichiro, Hochegger, H., Saberi, A., Taniguchi, Y., Takeda, S. 2006. Differential usage of non-homologous end-joining and homologous recombination in double strand break repair. DNA Repair, 5(9-10): 1021-1029. doi:10.1016/j.dnarep.2006.05.022.
  • Stadtman, E. R., & Berlett, B. S. 1997. Reactive oxygen-mediated protein oxidation in aging and disease. Chem. Res. in Toxic., 10(5): 485-494.
  • Stiff, T., O’Driscoll, M., Rief, N., Iwabuchi, K., Löbrich, M., Jeggo, P. A. 2004. ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation. Cancer Res., 64(7): 2390-2396. doi:10.1158/0008-5472.CAN-03-3207
  • Stoker, G. 1902. Ozone in Chronic Middle-ear Deafness. The Lancet, 160(4131): 1187-1188.
  • Strickland, A. F., Perkins, W. S. 1995. Decolorization of continuous dyeing wastewater by ozonation. Text. Chem. Color., 27(5): 11-15.
  • Sweet, F., Kao, M., Lee, S., Hagar, W., Sweet, W. 1980. Ozone selectively inhibits growth of human cancer cells. Scie., 209(4459): 931-933. doi:10.1126/science.7403859 %J Science.
  • Tang, Z. m., Liu, Y. y., Ni, D. l., Zhou, J. j., Zhang, M., Zhao, P., Bu, W. 2020. Biodegradable Nanoprodrugs:“Delivering” ROS to Cancer Cells for Molecular Dynamic Therapy. Adv. Mat., 32(4): 1904011. doi:10.1002/adma.201904011.
  • Tığlı Aydın, R. S., Kazancı, F. 2018. Synthesis and characterization of ozonated oil nanoemulsions. J. Ame. Oil Chem. Soc., 95(11): 1385-1398. doi:10.1002/aocs.12150.
  • Travagli, V., Zanardi, I., Valacchi, G.,Bocci, V. 2010. Ozone and ozonated oils in skin diseases: A Review. 2010.
  • Van Meir, 1996. Hypoxia-mediated selection of cells with diminished apoptotic potential in solid tumours. Neurosurg., 39(4): 878-879.
  • Yalçın, Y., Tekin, I. O., Aydın, R. S. 2021. Influence of Ozonized Oil Nanoemulsions on B-16 Melanoma Cells: An in Vitro Study. J. Biomat. and Nanobiotech., (12): 49-56. doi:doi: 10.4236/jbnb.2021.124005.
  • Young, S., Marshall, R., Hill, R. 1988. Hypoxia induces DNA overreplication and enhances metastatic potential of murine tumor cells. Proce.Natio. Acad. Sci., 85(24): 9533-9537. http:doi.org/10.1073/pnas.85.24.9533.
There are 31 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Research Articles
Authors

Yeliz Yalçın 0000-0003-1816-4161

İshak Özel Tekin 0000-0002-9969-4254

Rahime Seda Tığlı Aydın 0000-0002-4184-5320

Project Number 2016-39971044-02
Publication Date June 1, 2022
Published in Issue Year 2022 Volume: 12 Issue: 1

Cite

APA Yalçın, Y., Tekin, İ. Ö., & Tığlı Aydın, R. . S. (2022). Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells. Karaelmas Fen Ve Mühendislik Dergisi, 12(1), 38-46. https://doi.org/10.7212/karaelmasfen.985125
AMA Yalçın Y, Tekin İÖ, Tığlı Aydın RS. Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells. Karaelmas Fen ve Mühendislik Dergisi. June 2022;12(1):38-46. doi:10.7212/karaelmasfen.985125
Chicago Yalçın, Yeliz, İshak Özel Tekin, and Rahime Seda Tığlı Aydın. “Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells”. Karaelmas Fen Ve Mühendislik Dergisi 12, no. 1 (June 2022): 38-46. https://doi.org/10.7212/karaelmasfen.985125.
EndNote Yalçın Y, Tekin İÖ, Tığlı Aydın RS (June 1, 2022) Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells. Karaelmas Fen ve Mühendislik Dergisi 12 1 38–46.
IEEE Y. Yalçın, İ. Ö. Tekin, and R. . S. Tığlı Aydın, “Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells”, Karaelmas Fen ve Mühendislik Dergisi, vol. 12, no. 1, pp. 38–46, 2022, doi: 10.7212/karaelmasfen.985125.
ISNAD Yalçın, Yeliz et al. “Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells”. Karaelmas Fen ve Mühendislik Dergisi 12/1 (June 2022), 38-46. https://doi.org/10.7212/karaelmasfen.985125.
JAMA Yalçın Y, Tekin İÖ, Tığlı Aydın RS. Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells. Karaelmas Fen ve Mühendislik Dergisi. 2022;12:38–46.
MLA Yalçın, Yeliz et al. “Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells”. Karaelmas Fen Ve Mühendislik Dergisi, vol. 12, no. 1, 2022, pp. 38-46, doi:10.7212/karaelmasfen.985125.
Vancouver Yalçın Y, Tekin İÖ, Tığlı Aydın RS. Nano Ozonized Oil Trigger ROS Production and ɣH2AX Cell Positivity of B-16 Melanoma and OV-90 Ovarian Cells. Karaelmas Fen ve Mühendislik Dergisi. 2022;12(1):38-46.
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