Phenolic composition of bark tannin from Taurus Cedar (Cedrus libani)

Year 2017, Volume: 17 Issue: 4, 594 - 602, 20.12.2017

Oktay Gönültaş , Nur Sarıalan

https://doi.org/10.17475/kastorman.369041

Cited By: 1

Abstract

Aim of study: In this study, tannin extraction from waste
barks was performed which occur as a result of logging production of Taurus
cedar tree (Cedrus libani A.
Rich.)  that grows naturally in our
country. The phenolic composition of the cedar bark tannin was introduced by
analytical methods and FTIR analysis.

Area of study: Extraction of tannin from waste wood bark,
characterization of phenolic compound and determination of industrial use
possibilities.

Material and Methods: The bark sample of Taurus cedar from Adana
district was extracted from bark:water ratio of 1:8 (w/w) at 70 °C for 1 hour.
Extraction yield, pH and stiasny number values were determined at the extract
solution. Then, phenolic composition of the tannin was investigated by total
phenol content, butanol:HCl assay, DNS reducing sugar method, and FTIR
analysis.

Main results: As a result of extraction, tannin yield and
stiasny number value were determined 8.99 % and 28.85, respectively. The
results of total phenol content were 15.20 mg/g in ether phase and 27.77
aqueous phase. According to butanol-HCl assay, 7.61 mg/g proanthocyanidin
content was found and 41.77 mg/g reducing sugar power were assayed by DNS
reducing sugar method. FTIR spectra of the tannin was observed that
presence of the characteristic peak for condensed tannin at 1605 cm^-1.

Research highlights: Phenolic compound analysis methods and FTIR
analysis revealed that the the bark tannin of taurus cedar contained flavonoid
units, which are monomeric building units of condensed tannins.

Keywords

Taurus cedar, Bark, Tannin, FTIR

Toros Sediri (Cedrus libani) kabuk taneninin fenolik bileşimi

Abstract

Çalışmanın amacı: Bu
çalışma kapsamında ülkemizde doğal olarak yetişen Toros sediri (Cedrus libani A. Rich.) ağaçlarının
üretim kesimi sonucunda ortaya çıkan atık kabuklarından tanen ekstraksiyonu
gerçekleştirilmiştir. Sedir kabuk taneni fenolik bileşimi analitik yöntemler ve
FTIR analizi ile ortaya konulmuştur.

Çalışma alanı:
Atık ağaç kabuklarından tanen eldesi, ekstraktın fenolik bileşik
karakterizasyonu ve endüstriyel kullanım olanaklarının belirlenmesidir.

Materyal ve Yöntem: Adana bölgesinden temin edilen sedir kabuğu örnekleri, 1:8 kabuk çözücü
oranında, 70°C’de 1 saat sıcak suda ekstrakte edilmiştir. Ekstrakt
çözeltilerinde verim, pH ve stiasny sayısı değerleri belirlenmiştir. Ardından
elde edilen tanenin fenolik bileşimi toplam fenol tayini, bütanol:HCl yöntemi,
DNS indirgen şeker tayini ve FTIR analizi ile incelenmiştir.

Sonuçlar: Ekstraksiyon sonucunda % 8.99 verim ve 28.85 stiasny
sayısı değeri belirlenmiştir. Toplam fenol tayinini sonuçlarına göre eter fazı
15.02 mg/g ve su fazı 27.77 mg/g; bütanol HCl yöntemiyle 7.61 mg/g
proantosiyanidin ve DNS indirgen şeker tayini sonucunda 41.77 mg/g değerleri
elde edilmiştir. Tanen örneğinin FTIR spektrumu incelendiğinde kondanse
tanenler için karakteristik 1605 cm^-1 piki görülmektedir.

Araştırma vurguları: Fenolik
bileşik analiz metotları ve FTIR analizi ile toros sediri kabuk taneninin
kondanse tanen yapıtaşı monomerik flavonoid birimleri içerdiği ortaya
konulmuştur.

Keywords

Toros Sediri, Kabuk, Tanen, FTIR

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