MAGNOLİA BARK EKSTRAKTI UYGULAMASININ YAŞLI FARE KALP FONKSİYON YETERSİZLİĞİNDEKİ İYİLEŞTİRİCİ ETKİLERİ

Year 2024, , 227 - 234, 29.04.2024

Simge Ünay , Irem Aktay , Belma Turan

https://doi.org/10.18229/kocatepetip.1302307

Abstract

AMAÇ: Manolya ağacı (Magnolia) ekstraktının ek besin olarak kullanılmasının memelilerde çeşitli organlar ve dokular üzerinde anti-oksidan etkiler gösterdiği çeşitli çalışmalarla gösterilmiş olmasına karşın, fizyolojik yaşlanma ile ilişkili olarak gözlenen kalp yetersizliklerinde koruyucu etkisi ile ilişkili bir çalışma mevcut değildir. Bu nedenle bu çalışmada, magnolol ve honokiol bileşenleri içeren manolya ağacı ekstratı kompleksinin (MAHO-C) yaşlı farelerin (24 aylık) yetersiz kalp fonksiyonu üzerinde pozitif etkileri olup olmadığının incelenmesi hedeflenmiştir.
GEREÇ VE YÖNTEM: Bir grup yaşlı farelere (erkek BALB/c) standart fare yeminin yanı sıra MAHO-C (400 mg/kg/gün, 12 hafta boyunca oral olarak) takviyesi yapılırken, ikinci grup yaşlı fareler sadece standart yem ile beslenmiştir. Beslenme süresi bitiminde, farelerden in situ elektrokardiyogram (EKG) ölçümleri ve izole edilen kalp dokularında oksidatif stres ve antioksidan sistem parametrelerini kapsayan biyokimyasal analizler yapılmıştır.
BULGULAR: MMAHO-C takviyesi alan yaşlı farelere ilişkin EKG’lerde yapılan analizlerde, takviye almamış yaşlı farelerle karşılaştırıldığında, P-dalgası süresi, QRS-kompleksi süresi ve QT-aralığı gibi önemli EKG parametrelerinde düşüşler ile kalp atım hızında artma gözlenmiş olup bu değişimlerin istatistiksel olarak anlamı düzeyde olduğu gözlenmiştir. Kalp dokusunda yapılan biyokimyasal analizler, MAHO-C takviyeli yaşlı fare kalp dokularında arilesteraz aktivitesinde ve toplam antioksidan seviyesinde istatistiksel olarak anlamlı seviyelerde artma gözlenmiştir. Ek olarak, ek takviye alan gruplarda almayanlarla karşılaştırıldığında, reaktif oksijen türlerinin (ROS) kaynağı olan mitokondriyal enzim süksinat dehidrogenazın aktivitesinde ve toplam oksidan seviyesinde oranında istatistiksel olarak azalmalar ile ATP/ADP oranında istatistiksel olarak artış gözlenmiştir.
SONUÇ: Verilerimiz, MAHO-C takviyesinin fizyolojik yaşlanma ile ilişki kalp yetersizliklerinde özellikle antioksidan savunma sistemini ve mitokondriyi etkileyerek kalbin hem elektriksel ve hem de biyokimyasal özellikleri üzerinde pozitif etkiler oluşturabildiğini göstermektedir.

Keywords

Antioksidant, Beslenme destekleri, Bifenolik bileşikleri, Kalp, Oksidatif stres

Supporting Institution

Lokman Hekim Üniversitesi Bilimsel Araştırma Projeleri

Project Number

202AP404-2022

AMELIORATIVE EFFECTS OF MAGNOLIA BARK EXTRACT CONSUMPTION IN ELDERLY MICE CARDIAC DYSFUNCTION

Abstract

OBJECTIVE: It has been shown that to use Magnolia tree bark extract as supplement nutrient provided an anti-oxidative effect in various mammalian tissues and organs by several studies, however, there is no data associated with its cardioprotective role in physiological aging-related heart disorders. Therefore, here, we aimed to investigate whether Magnolia tree bark extract, containing magnolol and honokiol components (MAHO-C), has positive effects on heart dysfunction from aged mice (24-month-old).
MATERIAL AND METHODS: One group of aged mice (male BALB/c) was fed with a standard mouse chow plus MAHO-C (400 mg/kg/day, for 12 weeks with intragastrical), while the second group of aged mice was fed only with the standard chow. Following the end of the supplementation period, in situ electrocardiogram (ECG) measurements and biochemical analyzes associated oxidative stress and antioxidant system parameters of the isolated heart tissues from all mice were performed.
RESULTS: When compared to the nonsupplemented aged mice, the analysis performed in the ECGs of the aged mice supplemented with MAHO-C showed statistically significant decreases in the durations of P-waves, QRS-complexes, and QT-intervals as well as increases in the heart rates. The biochemical analysis of the heart tissues showed that the activity of arylesterase and total antioxidant levels in the heart tissues of the aged mice supplemented with MAHO-C were significantly high compared to those of untreated aged mice. In addition, when compared to the nonsupplemented group, there were significant decreases in the activity of the mitochondrial enzyme succinate dehydrogenase, one of source of reactive oxygen species (ROS) and the total oxidant level with a significant increase in the ratio of ATP to ADP in the heart samples of the aged mice supplemented with MAHO-C.
CONCLUSIONS: Overall, our data suggest that MAHO-C supplementation provides significant positive effects on both electrical and biochemical properties of the insufficiently functioning hearts from the physiological aged mice, particularly through affecting the antioxidant defense system and mitochondria.

Keywords

Antioxidant, MAHO-C, Phenolic compounds, Aging, Oxidative stress

Project Number

202AP404-2022

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