PROTECTIVE EFFECT OF NIGELLA SATIVA OIL ON CARBOPLATIN INDUCED NEPHROTOXICITY IN RATS

Year 2020, , 236 - 243, 01.07.2020

Züleyha Erişgin , Melahat Atasever , Muserref Seyma Ceyhan Suna Omeroglu Kadir Cetinkaya

https://doi.org/10.18229/kocatepetip.581590

Abstract

OBJECTIVE: Carboplatin is a commonly used chemotherapeutic agent. Kidneys are an important organ affected by the adverse effects of chemotherapeutic agents. This study aimed to investigate the protective effect of Nigella sativa oil (NSO) against kidney damage due to carboplatin exposure.
MATERIAL AND METHODS: All animals (n=24 female wistar-albino rats) were divided into four groups; 4 ml/kg saline was intraperitoneally (i.p.) administered on day one and two in the first group. 4 ml/kg NSO on day one and 4 ml/kg saline on day two was i.p. administered in the second group.4 ml/kg saline on day one and 80 mg/kg carboplatin on day two was i.p. administered in the third group.4 ml/kg NSO on day oneand 80 mg/kg carboplatin on day two was i.p. administered inthe fourth group. Rats were sacrificed at the end of day two and renal tissues were fixed in neutral formalin. Histopathologicchanges and apoptotic index (AI) were evaluated.
RESULTS: While an increase was observed in the apoptotic index of carboplatin+saline group compared to the control group, no significant differences were found in the carboplatin+saline and carboplatin+NSO group. In the histopathological evaluation, degeneration in the proximal and distal tubular epithelium and glomerular capillary glomus bodies, congestion in the vascular formations between the tubules, increase in collagen fiber density in the tunica adventitia of intraglomerular, preglomerular, intertubular and vascular formations, and sporadic basal disintegration due to Periodic Acid Schiff (PAS) reaction were observed in the carboplatin+saline group. In the carboplatin+NSO group, degenerative changes in some areas of tubular structures continued while it was observed that glomerular structures were more regular. It was observed that sclerotic change was fewer in the carboplatin+NSO group than in the carboplatin+saline group. It drew attention that basal membranes were more regular in the carboplatin-nigella sativa oil group as a result of PAS reaction.
CONCLUSIONS: NSO, is used as a spice, may have a protective effect on carboplatin induced nephrotoxicity.

Keywords

Apoptosis, Carboplatin, Kidney, Nigella Sativa Oil, Rat

Supporting Institution

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Project Number

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Thanks

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SIÇANLARDA KARBOPLATİN İLE İNDÜKLENEN NEFROTOKSİSİTE ÜZERİNE NİGELLA SATİVA YAĞININ KORUYUCU ETKİLERİ

Abstract

AMAÇ: Karboplatin yaygın kullanılan bir kemoteröpatik ajandır. Böbrekler kemoteröpatiklerin yan etkilerinden etkilenen önemli organlardır. Bu çalışmanın amacı karboplatin kullanımına bağlı oluşan böbrek hasarına karşı Nigella sativa yağının (NSY) koruyucu etkisini araştırmaktır.
GEREÇ VE YÖNTEM: Tüm hayvanlar (n=24 dişi wistar-albino sıçan) 4 gruba bölündü; birinci grupta 4 ml/kg serum fizyolojik (SF) 1 ve 2.gün uygulandı. İkinci gruba ilk gün 4 ml/kg NSY ve 4 ml/kg SF 2. Gün i.p. verildi. Üçüncü gruba 1.gün 4 ml/kg SF ve 2.gün ise karboplatin 80 mg/kg i.p. uygulandı. Dördüncü gruba 1.gün NSY ve 2. gün ise karboplatin 80 mg/kg i.p. uygulandı. İkinci günün sonunda sıçanlar sakrifiye edildi ve böbrek dokuları nötral formalin ile fikse edildi. Histopatolojik değişiklikler ve apoptotik index (AI) değerlendirildi.
BULGULAR: Apoptotik indexte, karboplatin+SF grubunda kontrol grubuna göre artış görülürken, karboplatin+ NSY grubu ile anlamlı bir fark görülmemiştir. Histopatolojik değerlendirmede ise; Karboplatin+SF grubunda proksimal ve distal tubul epitelinde, glomerular kapiller yumaklarında dejenerasyon, tubuller arasında bulunan vasküler oluşumlarda konjesyon, intraglomerular, periglomerular, tubuller arası ve vasküler oluşumların tunika adventisyasında kollagen lif yoğunluğunda artış, Periyodik Asit Schiff (PAS) reaksiyonu sonucu yer yer basal membran bütünlüğünün bozulduğu görülmüştür. Karboplatin+ NSY verilen grupta ise bazı alanlardaki tubul yapılarında dejeneratif değişikliklerin devam ettiği görülürken glomerul yapılarının daha düzenli olduğu gözlemlenmiştir. Karboplatin+ NSY verilen grupta karboplatin+SF verilen gruba göre sklerotik değişimlerin daha az olduğu gözlendi. PAS reaksiyon sonucu karboplatin+ NSY verilen grupta basal membranların daha düzenli bir yapıda olduğu görüldü.
SONUÇ: Baharat olarak da kullanılan nigella sativanın karboplatin ile indüklenen nefrotoksisite üzerine koruyucu etkileri olabilir.

Keywords

Apoptozis, Böbrek, Karboplatin, Nigella Sativa Yağı, Sıçan

Project Number

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