TİP 1 DİYABETES MELLİTUS OLUŞTURULAN SIÇANLARIN BEYNİNDE LEPTİN MİKTARI AZALMAKTADIR

Year 2019, Volume: 20 Issue: 2, 91 - 97, 29.04.2019

Erhan Şahin , Öykü Özcan Ezgi Bektur Cengiz Bayçu Ümide Özkay Demir , Özgür Devrim Can , Varol Şahintürk

https://doi.org/10.18229/kocatepetip.408118

Abstract

AMAÇ: Leptin hormonu, iştah ve vücut metabolizmasının düzenlenmesinde önemli görevler üstlenmekte ve başlıca yağ dokusunda sentezlenmektedir. Leptinin yağ dokuda sentezlendikten sonra koroid pleksus aracılığı ile beyne taşındığı bilinmektedir. Bu çalışmanın amacı Tip 1 diyabetes mellitus oluşturulan sıçanların beyninde leptin ifadesinin ve miktarının araştırılmasıdır.

GEREÇ VE YÖNTEM: Çalışmamızda toplam 14 adet yetişkin, erkek Wistar Albino sıçan 2 eşit gruba ayrıldı (n=7). Kontrol grubuna hiçbir uygulama yapılmadı. Diyabetes mellitus grubundaki hayvanlara ise tek doz (55 mg/kg) streptozotosin intraperitoneal olarak verildi ve kan glukoz seviyesi >280 mg/dL ölçüldüğünde Tip 1diyabetes mellitus geliştiği kabul edildi. Deney sonunda alınan beyin örnekleri %10’luk formaldehit ile fikse edildikten sonra rutin doku takip işleminin ardından alınan kesitlere leptin immünohistokimyasal boyaması uygulandı. Beyin dokularında western blot yöntemi ile leptin miktarına bakıldı.

BULGULAR: Tüm gruplara ait beyin kesitlerinde sadece koroid pleksusta leptin boyanması saptandı. Buna göre, leptin boyanmasının Tip 1 diyabetes mellitus geliştirilen sıçanlarda azaldığı saptandı. Western blot ile Tip 1 diyabetes mellitus grubunda leptin miktarının belirgin olarak azaldığı saptandı.

SONUÇ: Bu çalışmayla tokluk hissini oluşturmak üzere koroid pleksus üzerinden beyne geçen leptin hormonu ile diyabet arasında yakın bir ilişki olduğu ve bu hormonun diyabetle azaldığı gösterilmiştir. Bu çalışmadan yola çıkarak Tip 1 diyabet ve leptin hormonu ilişkisi derinlemesine irdelenmelidir.

Keywords

Diyabetes mellitus, beyin, leptin, koroid pleksus

LEPTIN QUANTITY DECREASES İN TYPE 1 DIABETES MELLITUS INDUCUED RATS BRAIN

Abstract

OBJECTIVE: Leptin hormone plays an important role in regulation of appetite and body metabolism and is mainly synthesized in fat tissue. It is known that leptin is transported to the brain via choroid plexus after synthesized in the fat tissue. The aim of this study is to investigate the amount and expression of leptin in the brain of rats with type 1 diabetes mellitus.

MATERIAL AND METHODS: A total of 14 adult male Wistar Albino rats were divided into 2 equal groups (n = 7). No application was made to the control group. A single dose (55 mg/kg) of streptozotocin was administered intraperitoneally to the animals of the diabetes mellitus group and it was considered that type 1 diabetes mellitus developed when blood glucose level > 280 mg/dL. Brain samples taken at the end of the experiment were fixed with %10 formaldehyde and then subjected to routine tissue monitoring followed by leptin immunohistochemical staining. The amount of leptin was determined by western blotting in brain tissues.

RESULTS: In all groups leptin staining was detected only in the choroid plexus of rat brains. According to this knowlodge, it was determined that leptin staining decreased in type 1 diabetes mellitus developed rats. The decreased leptin in the type 1 diabetes mellitus group was showed by western blot too.

CONCLUSIONS: Type 1 diabetes reduces the expression and amount of leptin in the choroid plexus.

Keywords

Diabetes mellitus, brain, leptin, choroid plexus

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