Propiltiourasil Etken Maddesine Sahip Bir Antitiroid İlaç Olan Propycil®’in Antibakteriyel ve Mutajenik Etkisinin Belirlenmesi

Year 2017, Volume: 20 Issue: 4, 350 - 354, 01.08.2017

Mehmet Arslan , Nurcan Erbil , Taygun Timoçin , Rima Çelik Mehmet Tahir Hüsunet , Hasan Basri İla

https://doi.org/10.18016/ksudobil.273803

Abstract

Bu çalışma antitiroid ilaç Propycil®’in
çeşitli test bakterileri üzerindeki antibakteriyel aktivitesinin belirlenmesi
ve potansiyel mutajenik etkisinin Ames / Salmonella / Mikrozom test sistemi ile
saptanması amacıyla yapılmıştır. Antibakteriyel aktivite testleri esnasında
test bakterisi olarak Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa
ATCC 9027, Bacillus megaterium DSM 32 ve klinik izolat olan Enterobacter
aerogenes, Escherichia coli, Klebsiella pneumoniae kullanılmıştır.  Mutajen etki çalışmalarında ise Ames Testi
tercih edilmiş olup, Salmonella typhimurium TA 98 ve TA 100 suşları
kullanılmıştır. Elde edilen bulgular neticesinde Propycil®’in test edilen
bakterilerinden sadece Klebsiella pneumoniae’ye karşı 13,35 mm ve Pseudomonas
aeruginosa ATCC 9027’ye karşı ise 15,46 mm inhibisyon zonu oluşturduğu tespit
edilmiştir. Ames testi sonucunda denenen dozların hiç biri TA 98 suşu üzerinde
çerçeve kayması mutasyonuna neden olmazken, TA 100 suşu üzerinde denenen en
yüksek doz hariç diğer tüm dozlar baz çifti değişimi mutasyonuna sebep olduğu
belirlenmiştir.

Keywords

Propycil®, antibakteriyel etki, mutajen etki, Salmonella typhimurium, Ames testi

Determination of the Antibacterial Activity and Mutagenic Effect of the Antithyroid Drug Propycil®, Propylthiouracil

Abstract

In this study, antibacterial activities of Propycil®
which is a antithyroid drug were examined for its antibacterial activity against
test bacteria and its potencial mutagenic activity on organism. Antibacterial
activity were tested against Staphylococcus
aureus ATCC 6538, Pseudomonas aeruginosa
ATCC 9027, Bacillus megaterium
DSM 32, and Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae which are clinic isolates. Potencial
mutagenic activity of Propycil® were tested in TA 98 and TA 100 strains of Salmonella typhimurium using Ames test.
Consequently, it was observed that Propycil® was caused inhbition zones on Klebsiella pneumoniae 13,35 mm ve Pseudomonas aeruginosa ATCC 9027 15,46
mm. In the Ames test, while none of the doses tested caused frame shift
mutations on TA 98 strains, all other doses, except for the highest dose, was
determined to cause a base pair substitution mutation change on TA 100 strains.

Keywords

Salmonella typhimurium, Propycil®, antibacterial, mutagen, Ames tests

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