Kahramanmaraş Atmosferinde Bulunan Allerjen Fungusların Belirlenmesi

Year 2017, Volume: 12 Issue: 1, 31 - 34, 24.04.2017

Ekrem Kireçci , Gökhan Alagöz Sibel Dağlı

https://doi.org/10.17517/ksutfd.308453

Abstract

            Bu çalışmada, yerçekimine dayalı Petri-Plak
Metodu kullanılarak Kahramanmaraş ilinin atmosferinde bulunan mikrofungal
florası ve fungusların sebep olabilecekleri sağlık sorunları araştırılmıştır.
Atmosferdeki fungusların izolasyonu amacıyla Dichloran Rose Bengal Chloramphenicol
Agarlı Besiyerleri,
identifikasyon içinde Saboraud Dekstroz Agar kullanıldı. Üreme davranışları, koloni morfolojisi,
petri kutusunda alt ve üstten görünüşleri,
pigment oluşumu, spor
ve hifsel yapılarının mikroskobik incelenmesi gibi parametrelere göre tanımlama kriterleri dikkate
alınarak
identifikasyonları cins
düzeyinde gerçekleştirilmiştir. Örnekleme yaz mevsiminde 3 ay süreyle
yapılmıştır. Çalışma sonunda 10 ayrı cins tespit edildi. Fungus konsantrasyonu
en çok kuzey ve güney istasyonlarında görülmüş olup bunun nedeni olarak da bitki
örtüsü ve kirlilik düzeyi ile alakalı olduğunu söğleyebiliriz. Bunlardan Penicillum spp, Clodosporium spp,
Aspergillus spp. ve Alternaria spp, tüm örneklerde ve her istasyonda görülmüştür.
Potansiyel alerjen ve toksik olan bu fungusların ciddi sağlık sorunlara neden
olabileceği düşünülmektedir.

Keywords

Hava kaynaklı fungus, izolasyon, identifikasyon

Detection of Allergen Fungi in the Atmosphere of Kahramanmaraş

Abstract

  
         In
this study; based on the gravity Petri-mikrofungal flora in the atmosphere of
Kahramanmaras using the plate method and the health problems it can cause
fungus has been studied. The isolation of fungi in the atmosphere in order
Dichloran Rose Bengal Chloramphenicol Agar in Media, in the identification
Saboraud Dextrose Agar was used. Reproductive behavior, morphology, bottom and
top views in petri dishes, pigment formation, sports and considering defining
the criteria based on parameters such as microscopic examination of hifsel
structure identification was carried out at genus level. Sampling was carried
out in summer for 3 months. At the end of the study 10 different species were
identified. Fungi concentration has been observed in most northern and southern
station as it is because it is associated with the vegetation and pollution
levels can soyel. Of these, Penicillium
sp, Clodospori sp, Aspergillus sp. and Alterneri
sp. every month, and it has been seen at every station. Potential allergen
and is thought to be toxic, cause serious health problems of these fungi.

Keywords

Airborne fungi, isolation, identification

References

• 1. Brown RM, Larson DA, Bold HC. Airborne algae: their abudance and heterogeneity. Principles of Microbial Ecology 1966;143: 583-585. 2. Flannigan B, Mccabe EM, Mcgarry F. Allergenic and toxigenic microorganisms in houses, Journal of Applied Bacteriology Symposium Supplement 1991;70: 61-73. 3. Miller JD. Fungi as contaminants in indoor air. Athmospheric Environ 1992; 26A: 2163-72. 4.Burge HA. Airborne allergenic fungi. Classification, nomenclature, and distribution. Immunol Allergy Clin N Am 1989;9: 307-19. 5. Martin JP. Use of acid rose bengal and Streptomycin in the plate method for estimating soil fungi. Soil Sci 1950;69: 215-232. 6. Ellis MB, Ellis JP. Microfungi on land plants. An Identification handbook 1997. 7. David AS, Linda, LA, Sean PA. A mounting medium for use in Indoor Air Quality spore-trap analyses. Mycologia 2002; 94: 1087-1088. 8.Yoshida K, Ando M, Sakata T, Araki S. Environmental mycological studies on the causative agent of summer- type hypersentivity pneumonitis. J Allergy Clin Immunol 1988; 81: 475-83. 9.Güneser S, Atici A, Köksal, Yaman A. Mold allergy in Adana, Turkey. Allergol Immunopathol, 1994;22: 52-4. 10. Klich MA. Identification of Common Aspergillus Species. United States Department of Agriculture Agricultural Resorce Service, Southern Regional Research Center New Orleans, Louisiana USA. 2002. 11. Pitt JI. The genus Penicillium and its telemorphic States Eupenicillium and Talaromyces. Academic press. London 1979, 634 pp. 12.Samson RA, Pitt JI. Integration of Modern Taxonomic Methods for Penicillium and Aspergillus Classification. 4rd ed. Harwood Academic Publisers 2000. 13.Hasenekoğlu İ. Mikrofunguslar için Laboratuar Tekniği, Atatürk Üniversitesi, Kazım Karabekir Eğitim Fakültesi, 66, Erzurum 1990. 14.Hasenekoğlu İ. Toprak Mikrofungusları 1-7. Atatürk üniversitesi Yayınları No: 689. Kazım Karabekir Eğitim Fakültesi Yayınları No: 11 Erzurum 1991. 15. İmalı A, Yalçınkaya B, Koçak M, Koçer F. Çorum İli Atmosferinde Hava ile Taşınan Allerjen Funguslar. Elek Mikrob Derg 2008;3: 19-24. 16. Şen B, Asan A. Fungal flora in indoor and outdoor air of different residental houses in Tekirdağ City (Turkey): Seasonal distribution and relationship with climatic factors. Environ Monit Assess 2009;151: 209-219. 17. Özkara A, Ocak I, Korcan SE, Konuk M. Determination of fungal air spora in Afyonkarahisar, Turkey. Mycotaxon 2007;102: 199-202. 18. Agarwal MK, Shivpuri DN, Mukerji KG. Studies on the Allergenic Fungal Spores of the Delhi, India Metropolitan Area. J Allergy 1969; 44: 193-203. 19.Kaliner M, et al. Alerjik Rinit ve Astım. Gelisim Jama 1987;258: 2851- 2857. 20.Grath JJ, Wong WC, Cooley JD, Straus DC. Continually Measured Fungal Profiles in Sick Building Syndrome. Current Microbiol 1999; 38: 33- 36. 21. Chih P, Huey-jen S, Chia-Yin L. Characteristic of indoor and outdoor airborne fungi at suburban and urban homes in two Season. Sci Total Environ 2000; 253: 111-118. 22. Yang CS. Toxic Effects of Some Common Indoor Fungi. Enviros: The Healthy Building Newsletter 1994.