Semen pH ve likefaksiyon süresi motilite ve morfoloji üzerinde etkili midir?

Year 2021, Volume: 16 Issue: 2, 172 - 177, 07.07.2021

Mehmet Kutlu Demirkol , Sefa Resim , Neslihan Temiz Mustafa Bilal Hamarat , Osman Barut

https://doi.org/10.17517/ksutfd.828863

Abstract

AMAÇ
Lİkefaksiyon süresi ve semen pH değerlerinin sperm fonksiyonları ile ilişkili olup olmadığı konusunda yeterli çalışma bulunmamaktadır. Bu nedenle, bu çalışmada pH ve lİkefaksiyon süresi ile ana semen parametreleri arasında bir ilişki olup olmadığı değerlendirilmesi hedeflendi.
YÖNTEM ve GEREÇ
Veriler normal ve anormal olarak gruplara ayrıldı ve pH ve likefaksiyon süreleri açısından karşılaştırıldı. Örnekler ayrıca semen pH değerine göre asidik (<7.2), normal (7.2-8.0) ve bazik (> 8.0) ve likefaksiyon süresine göre normal sıvılaştırma (≤30 dak.) ve yavaş sıvılaştırma (> 30 dak.) şeklinde gruplandırıldı, ve daha sonra konsantrasyon, motilite ve morfoloji bozuklukları açısından karşılaştırıldı.
BULGULAR
600 semen örneğinden 400'ü (% 66.7) anormal, 200'ü (% 33.3) normal olarak gruplandırıldı. Anormal grubun medyan pH ve sıvılaşma süresi normal gruptan istatistiksel olarak daha yüksekti (her ikisi de, p <0,01). Semen pH ve likefaksiyon süreleri astenoospermi, teratoospermi ve astenoteratospermi gruplarında anlamlı olarak daha yüksekti (tümü için p <0.01). Bazik ph (> 8.0) grubunun konsantrasyonu, progresif motilitesi ve morfolojisi hem asidik hem de normal ph gruplarından anlamlı olarak daha düşüktü (hepsi için p <0.01). Bunun aksine, sıvılaşma zaman gruplarının morfolojileri arasında sadece anlamlı fark vardı (p = 0.02).
SONUÇ
Anormal ve normal gruplarda semen pH ve likefaksiyon süresi değerleri anlamlı derecede farklıydı ve bu fark astenoospermi, teratospermi ve astenoteratospermisi olanlarda belirgindi.Bu ilişkileri daha iyi anlamak için kontrol grubu olarak kanıtlanmış fertil erkeklerle çalışmalar yapılmalıdır.

Keywords

Likefaksiyon süresi, morfoloji, motilite, semen pH

Is seminal pH and liquefaction time effective on motility and morphology?

Abstract

Objective
There are not enough reports about whether liquefaction time and semen pH values are related to sperm functions. Therefore, this study aimed to evaluate whether there is a relationship between pH and liquefaction time and major semen parameters.
Material and Methods
The data were divided into groups as normal and abnormal and compared in terms of pH and liquefaction times. The samples were also grouped according to semen pH as acidic (<7.2), normal ( 7.2-8.0) and basic (>8.0) and liquefaction time as normal liquefying (≤30 min.) and slow liquefying (> 30 min.), and then were compared in terms of the normal/abnormal status of concentration, motility and morphology.
Results
Of the 600 semen samples, 400 (66.7%) were grouped as abnormal and 200 (33.3%) as normal. The median pH and liquefaction time of the abnormal group were statistically higher than the normal group (both, p <0,01). The seminal pH and liquefaction times were significantly higher in asthenozoospermia, teratozoospermia and asthenoteratozoospermia groups (for all, p <0.01). The concentration, progressive motility and morphology of the basic ph (>8.0) group was significantly lower than both acidic and normal ph groups (for all, p <0.01). Unlike, there was only significant difference between the morphologies of liquefaction time groups (p=0.02).
Conclusion
The seminal pH and liquefaction time values in the abnormal and normal groups were significantly different and this difference was evident in those with asthenozoospermia, teratozoospermia and asthenoteratozoospermia. To understand these relationships better, studies with proven fertile men as a control group should be performed.

Keywords

Liquefaction time, morphology, motility, semen pH

References

• 1. World Health O. WHO laboratory manual for the examination and processing of human semen. 5th ed ed. Geneva: World Health Organization; 2010.
• 2. Amelar RD, Hotchkiss RS. THE SPLIT EJACULATE: ITS USE IN THE MANAGEMENT OF MALE INFERTILITY. Fertility and sterility. 1965;16:46-60.
• 3. Tauber PF, Propping D, Schumacher GFB, Zaneveld LJD. Biochemical Aspects of the Coagulation and Liquefaction of Human Semen. Journal of andrology. 1980;1(6):281-8.
• 4. Mandal A, Bhattacharyya AK. Some preliminary observations on the liquefaction of human semen. Andrologia. 1985;17(3):228-33.
• 5. Mandal A, Bhattacharyya AK. Biochemical Parameters of Slowly Liquefying Human Ejaculates. Archives of Andrology. 1988;20(2):141-5.
• 6. Bhushan S, Pandey RC, Singh SP, Pandey DN, Seth P. Some observations on human semen analysis. Indian journal of physiology and pharmacology. 1978;22(4):393-6.
• 7. World Health O. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 3rd ed. ed. Cambridge [England] ;: Published on behalf of the World Health Organization by Cambridge University Press; 1992.
• 8. Banjoko SO, Adeseolu FO. Seminal Plasma pH, Inorganic Phosphate, Total and Ionized Calcium Concentrations In The Assessment of Human Spermatozoa Function. Journal of clinical and diagnostic research : JCDR. 2013;7(11):2483-6.
• 9. LG H WC, QD H, TY H. Semen pH and sperm morphological changes in less, weak sperm disease clinical significance. Contemporary Medicine. 2013;19:2.
• 10. Haugen TB, Grotmol T. pH of human semen. Int J Androl. 1998;21(2):105-8.
• 11. Harraway C, Berger NG, Dubin NH. Semen pH in patients with normal versus abnormal sperm characteristics. American Journal of Obstetrics and Gynecology. 2000;182(5):1045-7.
• 12. Zhou J, Chen L, Li J, Li H, Hong Z, Xie M, et al. The Semen pH Affects Sperm Motility and Capacitation. PloS one. 2015;10(7):e0132974.